Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,695,585 (G)7→8 intergenic (‑42/‑216) purL ← / → mltF phosphoribosylformyl‑glycineamide synthetase/membrane‑bound lytic transglycosylase F, murein hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,695,5781.G94.5% 253.6 / 8.0 73intergenic (‑35/‑223)purL/mltFphosphoribosylformyl‑glycineamide synthetase/membrane‑bound lytic transglycosylase F, murein hydrolase
Reads supporting (aligned to +/- strand):  ref base . (2/2);  new base G (33/36);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01

TACTCGGCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCC  >  NC_000913/2695434‑2695723
                                                                                                                                                 |                                                                                                                                                   
tACTCGGCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑‑ggggg                                                                                                                                              <  1:212790/150‑1 (MQ=255)
 aCTCGGCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑‑gggggg                                                                                                                                             <  1:811586/150‑1 (MQ=255)
    cGGCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑‑GGGGGGGgaa                                                                                                                                         <  1:702897/151‑1 (MQ=255)
      gCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑‑GGGGGGGgaaac                                                                                                                                       >  1:953244/1‑149 (MQ=255)
       cGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACg                                                                                                                                       >  1:884328/1‑151 (MQ=255)
          aaaTATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACggg                                                                                                                                     >  1:366749/1‑150 (MQ=255)
          aaaTATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACggg                                                                                                                                     <  2:370459/150‑1 (MQ=255)
          aaaTATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACggg                                                                                                                                     <  1:1200082/150‑1 (MQ=255)
           aaTATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCg                                                                                                                                   <  1:1157414/151‑1 (MQ=255)
                    aaCCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATaa                                                                                                                          <  1:215628/151‑1 (MQ=255)
                       cGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATaa                                                                                                                          >  2:373271/1‑148 (MQ=255)
                       cGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATaa                                                                                                                          >  1:804252/1‑148 (MQ=255)
                       cGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGa                                                                                                                       <  1:1048575/151‑1 (MQ=255)
                             ccTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATGCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCt                                                                                                                   <  2:40789/149‑1 (MQ=255)
                              cTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGAt                                                                                                                >  1:1208570/1‑151 (MQ=255)
                              cTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGAt                                                                                                                >  2:95539/1‑151 (MQ=255)
                                ggCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGAt                                                                                                                <  1:156805/149‑1 (MQ=255)
                                ggCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATgc                                                                                                              <  1:1001730/151‑1 (MQ=255)
                                   aGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCt                                                                                                           <  1:1014711/151‑1 (MQ=255)
                                          aaaCGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATACAGAATCCGATGCGCTGACGaaa                                                                                                    <  2:326339/151‑1 (MQ=255)
                                          aaaCGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGaaa                                                                                                    <  1:262291/151‑1 (MQ=255)
                                           aaCGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAAc                                                                                                   <  2:547225/151‑1 (MQ=255)
                                           aaCGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAAc                                                                                                   >  1:415707/1‑151 (MQ=255)
                                              gTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGt                                                                                                >  2:967212/1‑151 (MQ=255)
                                                gCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGtt                                                                                               <  2:1015062/150‑1 (MQ=255)
                                                 ccAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGttt                                                                                              <  2:771393/151‑1 (MQ=255)
                                                  cagcagTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGc                                                                                            <  2:736801/151‑1 (MQ=255)
                                                   agcagTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCg                                                                                           <  1:1113798/151‑1 (MQ=255)
                                                      agTTTGTTGAATCGGAATGCCGGCAGTGCAGGCGAACCCCGCAGAATTTCCAACATAAGTCTCCCGTCTTTCAAGCTTTCGGTGGTCCCAAGGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGt                                                                                          <  2:730337/150‑1 (MQ=255)
                                                          tgttgATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAAt                                                                                    >  2:121093/1‑151 (MQ=255)
                                                           gttgATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATa                                                                                   <  1:983640/151‑1 (MQ=255)
                                                              gATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTTCCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATaaa                                                                                 <  1:274947/150‑1 (MQ=255)
                                                              gATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATaaaa                                                                                <  2:782411/151‑1 (MQ=255)
                                                                ttCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATc                                                                              >  1:262701/1‑151 (MQ=255)
                                                                 tCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATc                                                                              >  1:524988/1‑150 (MQ=255)
                                                                     aaTGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCAt                                                                         >  2:22669/1‑151 (MQ=255)
                                                                       tGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCg                                                                       >  1:1221980/1‑151 (MQ=255)
                                                                        gCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCg                                                                       <  1:1855/150‑1 (MQ=255)
                                                                        gCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGt                                                                      >  2:1228309/1‑151 (MQ=255)
                                                                         ccGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTg                                                                     >  2:77143/1‑151 (MQ=255)
                                                                            aCAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAAt                                                                  >  1:210827/1‑151 (MQ=255)
                                                                                     gCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAAcgc                                                         >  1:868799/1‑151 (MQ=255)
                                                                                        aaCCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGt                                                       >  1:651864/1‑150 (MQ=255)
                                                                                           cACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTgccg                                                   >  2:197368/1‑151 (MQ=255)
                                                                                           cACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTgccg                                                   >  1:569036/1‑151 (MQ=255)
                                                                                           cACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTgccg                                                   >  1:412044/1‑151 (MQ=255)
                                                                                                  aaTTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATgg                                            <  2:855554/151‑1 (MQ=255)
                                                                                                  aaTTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATgg                                            >  1:237736/1‑151 (MQ=255)
                                                                                                       ccATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCt                                          <  2:821405/148‑1 (MQ=255)
                                                                                                       ccATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGta                                       <  2:50590/151‑1 (MQ=255)
                                                                                                        catcatAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGtaa                                      <  2:717832/151‑1 (MQ=255)
                                                                                                         atcatAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGtaat                                     <  2:405127/151‑1 (MQ=255)
                                                                                                              aaGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGt                                 <  1:347968/150‑1 (MQ=255)
                                                                                                                 tctctcGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGcc                             >  2:701213/1‑151 (MQ=255)
                                                                                                                     tcGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCt                         >  2:397865/1‑151 (MQ=255)
                                                                                                                      cGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCAt                           <  2:953241/148‑1 (MQ=255)
                                                                                                                       gTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTg                        >  1:367995/1‑151 (MQ=255)
                                                                                                                          ttctAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGgcgc                    >  1:99013/1‑151 (MQ=255)
                                                                                                                           tctAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGgcg                     <  2:750653/149‑1 (MQ=255)
                                                                                                                             tAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAgg                 >  2:277777/1‑151 (MQ=255)
                                                                                                                             tAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAgg                 >  2:991912/1‑151 (MQ=255)
                                                                                                                             tAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAgg                 >  2:1197627/1‑151 (MQ=255)
                                                                                                                              aaGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCa                   <  1:916829/148‑1 (MQ=255)
                                                                                                                              aaGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAg                  <  1:95538/149‑1 (MQ=255)
                                                                                                                              aaGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGt                >  2:1049942/1‑151 (MQ=255)
                                                                                                                                gCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGttt              <  2:845670/151‑1 (MQ=255)
                                                                                                                                  tttCGTAGTACCCAA‑‑GGGGCGGGAAACGGGCGTCATTGTAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTa             >  2:1214521/1‑150 (MQ=255)
                                                                                                                                      ggTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCa         >  1:1131844/1‑150 (MQ=255)
                                                                                                                                       gtgtACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCaaa       <  2:804245/151‑1 (MQ=255)
                                                                                                                                        tgtACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCaa        >  1:1188953/1‑149 (MQ=255)
                                                                                                                                        tgtACCCAA‑‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCaaaa      <  2:1022227/150‑1 (MQ=255)
                                                                                                                                         gtACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAAt     <  2:224406/151‑1 (MQ=255)
                                                                                                                                         gtACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAAt     <  1:1148963/151‑1 (MQ=255)
                                                                                                                                           aCCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATg    <  2:1156322/150‑1 (MQ=255)
                                                                                                                                            cccAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGcc  >  2:595571/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TACTCGGCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCC  >  NC_000913/2695434‑2695723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: