Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,222,596	0	G	A	40.0%	85.5 / 43.5	71	F13F (TTC→TTT)	ymgD	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (37/5);  new base A (0/28);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 8.49e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACA  >  NC_000913/1222446‑1222740                                                                                                                                                         |                                                                                                                                                   ggCAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCg                                                                                                                                                    <  1:1119107/151‑1 (MQ=255)   cAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGaa                                                                                                                                                  <  1:499226/151‑1 (MQ=255)    aaCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGaa                                                                                                                                                  >  1:638654/1‑150 (MQ=255)       gCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAaaac                                                                                                                                               >  1:354003/1‑150 (MQ=255)        cTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAaaaca                                                                                                                                              >  1:604177/1‑150 (MQ=255)            cattacccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑g                                                                                                                                       <  2:735462/151‑1 (MQ=255)               tacccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGTCCAGGTCATTGGAGCGAAAACAACCA‑‑GGcc                                                                                                                                    >  2:343271/1‑151 (MQ=255)                acccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGcc                                                                                                                                    <  1:467864/150‑1 (MQ=255)                    attaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGcc                                                                                                                                >  2:728378/1‑150 (MQ=255)                    attaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCa                                                                                                                               <  2:290471/151‑1 (MQ=255)                    attaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCa                                                                                                                               <  2:1012320/151‑1 (MQ=255)                        ccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTa                                                                                                                            >  2:675022/1‑150 (MQ=255)                              caaTTTCTGCAACCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCaa                                                                                                                      >  2:626595/1‑150 (MQ=255)                                aTTTCTGCAACCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAAtt                                                                                                                   >  1:65216/1‑151 (MQ=255)                                 ttccggaaaCCTGTGCGCTGTCAGTTTTTC‑CAATTTCTTTGGTTTCTGCGAAAGTAATATGGTTTTCTGCCGGAAACCCTTTTTAAA‑TTTTGAAGTCCCGCGCCCAGGTAATTGGTGCAAAAAAAAACA‑‑GGCCCGCCAGTAAAGCAAAtt                                                                                                                   <  1:374096/143‑1 (MQ=255)                                  ttCTGCAACCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAAtttt                                                                                                                 >  2:434735/1‑151 (MQ=255)                                    cTGCAACCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAAtttttt                                                                                                               >  2:949116/1‑151 (MQ=255)                                        aaCCTGTGCGTTGTCATTTTTTG‑CCATTTCGTTGGTTTCTGCGCAAGAAATATAGGTTTCTGACGGCAACCCTTTTTAAA‑TATTTTAGTCCCGCCCCAAGGTCTTTGGTGAAAAAACAAACG‑‑GGCCCGCCAGTAAAGCAAAttttt                                                                                                                <  1:525121/146‑1 (MQ=255)                                        aaCCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTtcatc                                                                                                           >  2:1114182/1‑151 (MQ=255)                                          ccggtgCGCTGTCATTTTTTG‑CTTTTTCGTTGGTTTCTGCGAAAGTAATATAGTTTTCTGACGACAAACCTTTTTAAA‑TTTTGTAGTCCGGCGCCAGGGTCTTTGGTGCAAAAAAAAACG‑‑GGCCCGCCAGTAAAGCAAATTTTTtcatca                                                                                                          <  2:1226515/147‑1 (MQ=255)                                               gctcTGTCTTTTTTTG‑CCTTTTTGTTGTTTTCTGCACAAGTAATATAGGTTTCGGACGGAAAACTTTTTTAAA‑TATTGTAGTCCTGCCCCCAGTTCATTGGTGAAAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTcatcatcat                                                                                                      <  2:113275/146‑1 (MQ=255)                                               gcgtTGTAATTTTTTC‑CCTTTTCTTTGGCTTCTGCGAAAGAAATATGGTTTTCGGACGGCAACCCTTTTTAAA‑TTTTGTAGTCCGGCCCCCAGTTCATTGGTGAAAAAAAAAACG‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCAtt                                                                                                     <  1:655545/150‑1 (MQ=255)                                                  ggtgtcgTTTTTGCCCATTTCGTTGCTTTCTGCGAAATTAATATAGGTATCAGACGAAAACCCTTTTAAAA‑TATTGAAGCCCGGCGCCATGGTCATTGTAACAAAAAAAAAAA‑‑GCCCCGCCAGTAAAGCAAATTTTTTCATCATCAtt                                                                                                     <  2:495878/142‑1 (MQ=255)                                                   tGTCTGTTTTTG‑CCTTTTCGTTGGTTTCTGCGAAAGTAATATAGGTTTCGGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCAAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCt                                                                                                  <  1:128875/149‑1 (MQ=255)                                                   tGTCATTTTTTG‑CCTTTTCGTTGGTTTCTGCGCAATTAATATAGGTTTCTGCCGGCAAACCTTTTTTAA‑TTTTGTAGTCCTGCCCCAAGGTCATTGGTGCAAAAAAAAACG‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTcc                                                                                                   <  1:446402/148‑1 (MQ=255)                                                     tCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTAtt                                                                                              >  2:954853/1‑151 (MQ=255)                                                        ttttttG‑CCGTTTGTTTGGTTTTTGCGCAGTAAATTTTTTTTTCTGGCTGCAACCTTTTTTTAA‑TTTTTCGGTCCTGCACCC‑GGTTCTTGG‑GCTATAAAAAACATGGGGCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTc                                                                                            <  2:1230395/149‑1 (MQ=255)                                                         tttttG‑CTATTTCGTTGGTTTCTGCGAAATTAATATAGGTTTCTGACGGCAAACCTTTTTTAA‑TATTGGAGTCCGCCCCCAAGTTTTTTGGTGAAAAAAAAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCa                                                                                           <  2:478712/150‑1 (MQ=255)                                                          ttttG‑CTTTTTCGTTGGCTTCTGCAAAAGTAATATAGGTTTCTGACGGCAACCCTTTTTTAA‑TATTGTAGTCCTGCGCCAAGGTCTTGGGTGAAAAAACAAACA‑‑GCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAt                                                                                          <  2:147395/150‑1 (MQ=255)                                                                ccATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTAcc                                                                                    >  2:697199/1‑151 (MQ=255)                                                                ccATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTAcc                                                                                    >  2:44400/1‑151 (MQ=255)                                                                 cATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTAccc                                                                                   >  2:581841/1‑151 (MQ=255)                                                                   tttCGTTGGTTTCTGCGCAAGTAATATAGTTTTCGGACGGAAAACTGTTTTAAA‑TTTTGTAGCCCTGCGCCCAGTTCATTGGTGCAAAAAAAAACG‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCa                                                                                  <  1:241501/150‑1 (MQ=255)                                                                   tttCGTTGGCTTCTGCGCAAGTAATATAGGTTTCGGACGGCAACCCTTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGGGAAAAAACAAACA‑‑GCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTAcca                                                                                   <  1:432685/149‑2 (MQ=255)                                                                        ttGGTTTCTGCGAAAGTAATATGGTTTTCTGACGGCAAACCTTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCTTTGGTGCAAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAtt                                                                             <  1:1168255/150‑1 (MQ=255)                                                                        ttGGCTTCCGCGAAAGTAATATAGTTTTGTGACGGCAACCCTTTTTAAA‑TATTGAAGCCCGGCCCCCAGGTCATTGGTGAAAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAt                                                                              <  1:697208/149‑1 (MQ=255)                                                                         tGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTg                                                                            >  2:551247/1‑150 (MQ=255)                                                                          gttttCTGCGCAAGTAATATGGGTTGCTGCCGGAAAACTGTTTTAAA‑TTTTGTAGTCCTGCGCCCAGTTTTTTGGTGCAAAAACAAACG‑‑GCCCCGCCAGTAAAGCAAATTTTTTCAACATCATTCCTCATTCAAGTTTACCCAGAATTg                                                                            <  2:1082188/146‑1 (MQ=255)                                                                          gttttCTGCACAAGTAATATAGGTTTCTGACGGCAACCCTTTTTTAA‑TATTGTAGTCCTGCCCCCAGTTCTTGGGTGAAAAAACAAACA‑‑GCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTg                                                                            <  1:1010474/146‑1 (MQ=255)                                                                          ggCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGc                                                                           >  2:712158/1‑150 (MQ=255)                                                                          ggCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGc                                                                           >  1:774836/1‑150 (MQ=255)                                                                                  cgcAAGAAATATGGTTTTTTGACGGCAACCTTTTTTAAA‑TATTTTGGTCCGCCCCCCAGTTCATGGGTGCAAAAAAAAACG‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGt                                                                  <  1:343285/151‑1 (MQ=255)                                                                                     aaGTATTAGAGTTTTCGGCGGGCAACCTTTTTTTAA‑TTTTGTAGTCCTGCCCCAAGTTCATTGGAGAAAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTAc                                                                <  2:639716/150‑1 (MQ=255)                                                                                     aaGTAATATGGGTTTCGGACGGCAACCCTTTTTTAA‑TATTGTAGCCCTGCGCCAAGTTCTTTGGTGCAAAAAAAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTa                                                                 <  1:85605/149‑1 (MQ=255)                                                                                     aaGTAATATAGGTTTCGGACGGCAACCCTTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCAAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTa                                                                 <  1:812605/149‑1 (MQ=255)                                                                                                gTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACgg                                                    >  1:21645/1‑151 (MQ=255)                                                                                                 tttCTGCCGTCAACCCTTTTTTAA‑TTTAGTAGCCCCGCCCCCAGGCTTTTGGTGAAAAAACAAACG‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACg                                                     <  1:1099375/149‑1 (MQ=255)                                                                                                  ttCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCTGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCCCCCGTACCGCCAATCACGGc                                                   >  2:290092/1‑150 (MQ=255)                                                                                                      gACGGCAACCTGTTTTAAA‑TTTTGTAGTCCTGCGCCCAGTTCTTGGGTGAAAAAACAAACG‑‑GCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCtt                                              <  1:1074459/151‑1 (MQ=255)                                                                                                       aCGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCttt                                             >  1:963961/1‑151 (MQ=255)                                                                                                          ggaaaCCTTTTTTAATTTTTGTAGCCCTGCCCCCAGTTCTTGGGGGAAAAAAAAACCG‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTa                                            <  1:84334/148‑1 (MQ=255)                                                                                                           cAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAAt                                          >  1:953735/1‑150 (MQ=255)                                                                                                            aacctttttttAA‑TATTGTACCCCGGCCCCCAGGTCTTTGGTGAAAAAAAAAACG‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCAc                                                 <  1:1186200/136‑1 (MQ=255)                                                                                                              aCCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGcc                                      >  1:678665/1‑151 (MQ=255)                                                                                                               ccGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGccc                                     >  1:1019000/1‑151 (MQ=255)                                                                                                                 gTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCt                                   >  2:95991/1‑151 (MQ=255)                                                                                                                  tttttAA‑TTTTGTAGCCCGGCCCCCAGGTCATTGGTGCAAAAAAAAACG‑‑GCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCt                                   <  1:338378/150‑1 (MQ=255)                                                                                                                  tttttAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCtt                                  >  2:482875/1‑151 (MQ=255)                                                                                                                   tttaAA‑TATTGTGGTCTTCCCCCCGGGTTATTGGTCAAAAAAAAAACG‑‑GCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCtt                                  <  1:1233165/150‑1 (MQ=255)                                                                                                                    tttAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCg                                >  1:1218155/1‑151 (MQ=255)                                                                                                                      tAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCg                                >  2:6971/1‑149 (MQ=255)                                                                                                                       aa‑TATTGTACCCCGCCCCCCAGCTCTTTGGCGAAAAAAAAAAGG‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAg                              <  1:728385/150‑1 (MQ=255)                                                                                                                                   ccTACGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCg                    >  2:269143/1‑149 (MQ=255)                                                                                                                                    cTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTc                 >  1:657740/1‑151 (MQ=255)                                                                                                                                      gcgcCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTc                 >  1:117707/1‑149 (MQ=255)                                                                                                                                        gcCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGa              >  2:646668/1‑150 (MQ=255)                                                                                                                                          ccAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACg           >  2:968537/1‑151 (MQ=255)                                                                                                                                               tCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCATGGCACCTTTAATCGCCCCTTCGAGGCCATTCCCGGTCAGTACGACa        >  2:447896/1‑149 (MQ=255)                                                                                                                                                 aTTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTg     >  1:895408/1‑150 (MQ=255)                                                                                                                                                  ttGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGAc   >  1:640057/1‑151 (MQ=255)                                                                                                                                                   tGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGAc   >  1:292142/1‑150 (MQ=255)                                                                                                                                                    ggTGCAAAAACAACCA‑‑GCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACa  <  1:832921/150‑1 (MQ=255)                                                                                                                                                         |                                                                                                                                                   GGCAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTG‑CCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAA‑TATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACA  >  NC_000913/1222446‑1222740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑