Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,413,7350AT42.0% 56.5 / 37.4 50Y2* (TAT→TAAydaCDUF1187 family protein, Rac prophage; putative double‑strand break reduction protein
Reads supporting (aligned to +/- strand):  ref base A (4/25);  new base T (21/0);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 3.76e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGG  >  NC_000913/1413588‑1413885
                                                                                                                                                      |                                                                                                                                                           
cAGTTTTACTTTCTTCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGAGTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTtata                                                                                                                                                           <  2:51810/150‑1 (MQ=255)
 aGTTTTACTTTCTGCGCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTtttat                                                                                                                                                          >  2:536762/1‑149 (MQ=255)
       aCTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAac                                                                                                                                                    <  1:1048150/150‑1 (MQ=255)
           tCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGAAAATTTATTCATTAACACCTc                                                                                                                                              >  1:266547/1‑151 (MQ=255)
              gCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTTAATTTTTTTCATGAAACCTcc                                                                                                                                             >  2:645493/1‑149 (MQ=255)
              gCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCa                                                                                                                                            <  2:649203/150‑1 (MQ=255)
                tctctGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTTAATTTTTTCATTAGAACCTCCctt                                                                                                                                          >  2:687367/1‑150 (MQ=255)
                   ctGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCatta                                                                                                                                         <  1:794903/148‑1 (MQ=255)
                     ggAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCattattatt                                                                                                                                   <  1:513312/151‑1 (MQ=255)
                     ggAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCattattat                                                                                                                                    <  2:670075/150‑1 (MQ=255)
                            ccTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTg                                                                                                                           <  1:421651/151‑1 (MQ=255)
                             cTGCTTTTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAg                                                                                                                             <  2:874763/148‑1 (MQ=255)
                                 ttcttTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTACATGAACACCTCCCTtttttttt                                                                                                                                  >  1:817548/1‑140 (MQ=255)
                                 ttcttTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTAAAAAAAAACCTTCACTATTTTTTTCAATgggt                                                                                                                        >  1:697499/1‑147 (MQ=255)
                                     tttttACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAAATTTTTAAAATGAAAACCCCCCTTTTTTTTTCCGGGGGtgggtt                                                                                                                    >  1:904546/1‑145 (MQ=255)
                                     tttttACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGttt                                                                                                                  <  1:731829/150‑1 (MQ=255)
                                      ttttACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAAATTTTTTTATGAAAAACCCCCCTATTAttttttgtgggttgtgt                                                                                                                    >  2:1109947/1‑135 (MQ=255)
                                        ttACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTAtt                                                                                                               <  2:670095/150‑1 (MQ=255)
                                         tACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTATATGAACACCTCCCTTATTTTTTTCatcgggttgtttttt                                                                                                                 >  1:17719/1‑135 (MQ=255)
                                           ccTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTACATGTACACCCCCCTTATTTTTTTCAATGGGTTGtttttt                                                                                                               >  1:380391/1‑147 (MQ=255)
                                           ccTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCAt                                                                                                           <  1:1102343/151‑1 (MQ=255)
                                            cTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTATATTAAAAACCCCCTTTTTTTTTTCAGTGGGTCGttttttt                                                                                                              >  2:542734/1‑146 (MQ=255)
                                            cTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTAAATTAAAACCTCCCTTTTTATTTTCCGTGGGTCGtttttttc                                                                                                             >  1:1037128/1‑146 (MQ=255)
                                                 agCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTAATAAACACCTACCTTTTTTTTTTCATGTGGTTGTTTTTTTCcttctt                                                                                                        >  2:1073302/1‑149 (MQ=255)
                                                   cATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAg                                                                                                   <  1:2306/151‑1 (MQ=255)
                                                      tttttCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTg                                                                                                 <  1:444150/150‑1 (MQ=255)
                                                       ttttCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTAAAAAACACCCCCCTTTTTTTTTTCTGTGGTTTGTTTTTTTCCTTTTTTGAGt                                                                                                  >  1:1067449/1‑148 (MQ=255)
                                                           cGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGcttct                                                                                            <  1:772348/150‑1 (MQ=255)
                                                              aTTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTTAATTTTTTAATTAACACCCCCCTTTTTTTTTTCTGTGGTTTGTTTTTTTCATTTTTTTTGTGCtttttttt                                                                                        >  2:249596/1‑151 (MQ=255)
                                                              aTTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTTATTTTATTAATGAACACATCCCTTATTTTTTTCCTGGGGTTGGTTTTTTctttcttttggaggtttttttt                                                                                         >  2:616191/1‑137 (MQ=255)
                                                                tCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAAATTTTTATATGTAAAACCCCCTTATTTTTTTTATGGGGTTGGTTATTTCCTTTTTTCAGTGCTTCttttt                                                                                        >  1:364178/1‑149 (MQ=255)
                                                                   gATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATATTTTTATAAGAAAACACCATTTATTTTTTCTATGGTTTGTTTTATTCTACTTTCTAGTGCCTttttttttttt                                                                                   >  2:1046190/1‑146 (MQ=255)
                                                                    aTTTGGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATAAATAAAAACATCCATTTTTTTTTTCAGTGGGTGGGTTTTTTTATCTTTTGAGTGGTTGtttttttctt                                                                                   >  2:379832/1‑145 (MQ=255)
                                                                       tCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGGAATTTTTTAAAAGAAAACCCCCACTATTTTTTTCCATGGGTCGTTTTTTTCTTTTTTTTAGTGCGTttttttttctt                                                                                   >  2:753933/1‑142 (MQ=255)
                                                                          ttatttttcaTTTTGAATATCTTGTCCATTTAGTAGAAGTGCCACCTTCCTTTTCAATAGTGGCGCTAATTTTATACATGAACACCTCCATTATTATTTCCAGGGGTTCTTTTATTCCATCTTTCGATTGCTTCTTTTTCATTTccac                                                                               <  2:880934/139‑1 (MQ=255)
                                                                               ttAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAAcc                                                                       <  1:1022052/150‑1 (MQ=255)
                                                                                 aGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCgg                                                                     <  2:904546/150‑1 (MQ=255)
                                                                                 aGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGt                                                                    <  1:458344/151‑1 (MQ=255)
                                                                                   aTTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTCATTTTTTATAAATAAACCCCCACTTTTATTTTCCCTAGGTCGTTTTTTTCCTCTTTTGTCGGCTTTTTTTTTTCTTCCACCCTAAcccg                                                                     >  1:276474/1‑146 (MQ=255)
                                                                                    ttttGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCCCTTCCACCATAACCg                                                                      <  2:747797/146‑1 (MQ=255)
                                                                                      ttGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCgg                                                                <  1:981593/150‑1 (MQ=255)
                                                                                      ttGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCgg                                                                <  1:300861/150‑1 (MQ=255)
                                                                                                tGTCCAGTTAGTAGGAGGGCCACCTTCCTTTTCAATAGTGGCGGTGATTTTTTTATTTAATAACTCCCTTCTTTTTTTTTGTTGTTCGGTTTTTTCTTTTTTCCCATGCTTCTTTTTTTCTTTCTCCCTAATCGGCTTggggtgaccct                                                       >  2:962340/1‑141 (MQ=255)
                                                                                                 gTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATcc                                                     <  2:141344/150‑1 (MQ=255)
                                                                                                  tCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTACATGAACAACCCCACTATTTTTTTCAAGTGGTCGTTTTTTTTATCTTTTTTGTTCTTTTTTTTTTCTTTCACCCCAACCCGGTCGGGGTTCTCATcc                                                     >  2:205122/1‑149 (MQ=255)
                                                                                                  tCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTAAATTAACACCCCCCTTTTTATTTTCAGTGGGTCGTTTTTTTCCTCTTTCTTGGGCTTCTTTTTTTCTTTCCCCATAACCAGTTTGGGGTTCGCATcc                                                     >  1:925962/1‑149 (MQ=255)
                                                                                                            agGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTAAAAGAACACCCCCCCTTTTATTTTCAGTGGGTTGTTTTTTTCTTTTTTTGAGGGCTTTTTTTTTTTTTCCCCCCTAAACCGGTTGGGGGTTGCATCCCTTACACtt                                            >  1:1170449/1‑148 (MQ=255)
                                                                                                              gAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCt                                        <  2:328860/150‑1 (MQ=255)
                                                                                                                gTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCgg                                     <  1:502659/151‑1 (MQ=255)
                                                                                                                  gCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTa                                   <  2:855017/151‑1 (MQ=255)
                                                                                                                             tttCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGa                          <  2:774537/149‑1 (MQ=255)
                                                                                                                                        gCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCAcc             >  1:382050/1‑151 (MQ=255)
                                                                                                                                                      ataCATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATgg  <  1:760778/151‑1 (MQ=255)
                                                                                                                                                      ataCATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATg   <  2:704889/150‑1 (MQ=255)
                                                                                                                                                      ataCATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATg   <  1:1209361/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                           
CAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGG  >  NC_000913/1413588‑1413885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: