Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,686	0	G	T	42.1%	43.4 / 18.4	57	intergenic (+40/+25)	yegJ/yegK	uncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (20/13);  new base T (8/16);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 6.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

GATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCG  >  NC_000913/2151538‑2151835                                                                                                                                                          |                                                                                                                                                        gATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑ttgtt                                                                                                                                                       <  1:311389/151‑1 (MQ=255)     tataCGCGCGGGGATAAAATTTACGGCGGTTATAACAATTATTCTTTTTTTTTTACCTATCCAAAAAAAGAAGACGACGAGCTAAAAGCAAAA‑ATAGTTTGCTTATCAGCCGCTATTTTTTTTTTTTTAAATGGGGGG‑TTG‑AA‑ttttt                                                                                                                                                       >  2:1048446/1‑148 (MQ=255)      ataCGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTCtt                                                                                                                                                  <  2:992500/151‑1 (MQ=255)             cgGGGATAAAATTTACGGCGGTTTTTCCATTTATCCCTTTTTTTTCGCCCATCCCAAAAAAGAAAGCGACGAGCTACAAGCAAAA‑CTTGTTTTCTCATCAGCCGATA‑TTTTTTTTTTTTAAAATGGGGG‑GTG‑GA‑ATTTTGtttttctaccc                                                                                                                                           >  1:1133451/1‑144 (MQ=255)             cgGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTCTTCAACCGc                                                                                                                                           <  1:778075/151‑1 (MQ=255)                      aaTTTACGGCGTTTATACCATTGATCCTTTATTGGTCCCCTATCAAAAAGAAGAAGCCGACGAGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTA‑AA‑TTGTTGTCTTCAACCGCCATGAGaaa                                                                                                                                  <  2:161064/151‑1 (MQ=255)                            cggcggTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑GTGTAA‑TTTTTTTTTTTAACccccctaggaa                                                                                                                                   >  1:867005/1‑138 (MQ=255)                                 gTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACT‑CTGcc                                                                                                                      >  1:745436/1‑151 (MQ=255)                                  ttATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TGG‑GA‑ATTTTTTCTTTAACCGACATGAGaaaatatatcttccc                                                                                                                      >  2:354279/1‑139 (MQ=255)                                       ccATTGTTCTTTTATTGGTCCCTTATCAAAAAAAAGAAGCCGACGAGCTAAGAGAAAAA‑TTAGTTCGCTAATCAGCCTTTA‑TATTTTTTTTTTAAACGGGGGG‑TTG‑AA‑TTGTTGTTTTCAACCGCCATGAGAAACTCACT‑CTGCCcaca                                                                                                                  <  1:1689/149‑1 (MQ=255)                                       caaTTGATCTTTTATTGGTCCCCTATCAAAAAGAAGAAGCCGACGAGTTAAGAGAAAAA‑TTATTTCGTTAATCAGCCTTTA‑TTTTTATTGTTAAAACTGGCGT‑TTA‑AA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACT‑CTGCCcaca                                                                                                                  <  1:810563/147‑1 (MQ=255)                                          ttGATCTTTTATTGGTCCCCTACCAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAA‑TTAGTCCGCTAATCAGCCTTTA‑TATTTATTTTTTAACCGGGCGG‑TTG‑AA‑TTTTTGTCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAgc                                                                                                                <  2:418414/148‑1 (MQ=255)                                          ttGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTTTTCAACCGCCATGAGAAAATCACT‑CTGCCCACACCGcc                                                                                                             >  1:463126/1‑151 (MQ=255)                                               ccTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTT‑AA‑TTTTTTTTTTCAAATGCCATGAAAAAATCACTCCTCCCcacacgac                                                                                                              >  2:352487/1‑142 (MQ=255)                                               ccTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑ATG‑AA‑TTGTTGTCTTCAACCGCCCTGAGAAAATCAAT‑CTGACCCCAACGcc                                                                                                             >  2:56044/1‑146 (MQ=255)                                                 tttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTTTTTTCTTTAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGcc                                                                                                       >  2:237572/1‑150 (MQ=255)                                                  tttttgtcccccTATCAAAAAAAAAAAGCCGA‑GAACAAAAAAAAAAATTTAGTTCCTAAACCACCCTTTT‑TTTTTTTTGTTAAAACGGGCGG‑TTA‑AA‑TTGTTGTCTCAAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCa                                                                                                      <  2:448919/140‑1 (MQ=255)                                                         tCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTTTTCAAACGCCATGAGGAAATCACT‑CTGCCCACAGCGCCAGTGGCCAAGtttt                                                                                               >  2:527434/1‑150 (MQ=255)                                                               accaaaaaGAAGAAGCCGACGGGCTAAGAGAAAAA‑TTATTTCGCTAATCAGCCTTTA‑TTTTTTTTGTTTAAACGGGGGT‑TTA‑AT‑TTTTTTTCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTa                                                                                              <  2:157232/141‑1 (MQ=255)                                                                   aaaaGAAGACGCCGACGAGATAAGAGAAAAA‑TTAGTTCGCTAATCAGCTTTTA‑TATTTATTGTTTAATCGGGGGT‑TTA‑AA‑TTTTTGTCTCAATCCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTatcat                                                                                          <  1:1064280/145‑1 (MQ=255)                                                                     aagaagaagCCGACGAGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGt                                                                                   >  1:741982/1‑150 (MQ=255)                                                                      agaagaagCCGACGAGCAAAGAGAAAAA‑TTAGTCCGCTAATCAGCCTTTA‑TTTTTTTTGTTAAAACTGGCGG‑TTA‑AA‑TTTTTGTCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTAc                                                                                 <  1:390174/151‑1 (MQ=255)                                                                       gaagaagCCGACGAGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTTTCTTCAACCCCCATGAGAAACTCACT‑CTGCCCCCAGCGCCAGTGCCAACGTTTTTTCATCATCCGTAc                                                                                 >  2:1123228/1‑150 (MQ=255)                                                                        aaaaagCGGACAACCAAAGAAAAAAA‑TTAGTTCGTTAATCGGCCTTTT‑TTTTTTTTTTTTAACCGGGCGT‑TTG‑AT‑TTGTTGTTTCAAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACg                                                                                <  1:1202647/147‑1 (MQ=255)                                                                           aagCCGACGAGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAAAGTTTTATCATCATCCGTACGCTCg                                                                            >  1:1177057/1‑151 (MQ=255)                                                                               cgacgaGCAAAAAGAAAAT‑TTAGTCCGATAACCACCCTTTA‑TTTTTATTTTTTAACCGGGGGG‑TTA‑AT‑TTGTTGTCTAAAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGttt                                                                         <  2:163288/150‑1 (MQ=255)                                                                                gacgaGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTCTTTAACCGCCCTGAGAAACTCACT‑CTTCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGgtt                                                                         >  2:602563/1‑149 (MQ=255)                                                                                    aGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTTTTGTCTTCAACCCCCATTAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCCAAGTTTTTTTATCATCCCTACGCTCGTTTaacccc                                                                   >  2:784681/1‑146 (MQ=255)                                                                                        aagaaaaaaa‑TTATTCCGTTAATCGGCCTTTA‑TTTTTATT‑TTTAAACCGGGGGTTTA‑AA‑TTTTTGTCTTAAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑c                                                              <  2:1218371/145‑1 (MQ=255)                                                                                        aaaagaaaaa‑TTATTCCGTTAACCAGCCTTTA‑TTTTTTTTTTTTAAACGGGGGG‑TTA‑AT‑TTTTTTTCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCgg                                                                 <  1:411395/143‑1 (MQ=255)                                                                                              aaaa‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGAc                                                         >  1:90207/1‑150 (MQ=255)                                                                                                   ttAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTT‑AA‑TTGTTGTCTTCAACCGCCATGAGAAAATCACT‑CTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGGCaaaa                                                     >  1:875294/1‑150 (MQ=255)                                                                                                    tAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTT‑AA‑TTTTTGTCTTCAACCGCCCTGAGAAACTCACT‑CTTCCCACAGCGCCAGTGCCCACGTTTTTTCATCCTCCGTACCCCCGTTTACCGCCGGG‑CTGGACaaaaa                                                    >  2:503750/1‑150 (MQ=255)                                                                                                    tAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTCTTCAAACGCCATGAGAAAATCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACaaaaa                                                    >  1:634580/1‑150 (MQ=255)                                                                                                         cGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑ATGTTGTCTTCAACCGCCCTGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑GTGGACAAAAACCGCtt                                              >  1:663603/1‑151 (MQ=255)                                                                                                            tAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTTTTTTCTTCAACCGCCATTAGAAACTCACT‑CTTCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCCTACGCTCGTTTACCGCCGGG‑CTGGACAAAAAATGCttt                                             >  2:214835/1‑149 (MQ=255)                                                                                                            tAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCttt                                             >  1:542179/1‑149 (MQ=255)                                                                                                             aaTCAGCCTTTA‑TATTTTTTGTTTAAACGGGCGT‑TTG‑AT‑TTGTTGTCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTaa                                           <  1:719035/150‑1 (MQ=255)                                                                                                              accacccGTTA‑TATTTTTTTTTAATCCGGGGGG‑TTA‑AA‑TTTTTCTTTACAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACaaa                                                      <  2:826752/133‑1 (MQ=255)                                                                                                                  gctgTTA‑TTTTTTTTGTTAAACCTGGCGT‑TTG‑AT‑TTTTTGTCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACa                                         <  2:147269/144‑1 (MQ=255)                                                                                                                   ctgTTA‑TTTTTATTTTTAATACGGGTGT‑TTG‑AC‑TGTTTGTCTAAACCCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAAtt                                      <  1:501729/147‑1 (MQ=255)                                                                                                                   ccGTTA‑TATTTATTGTTTAAACTGGCGG‑TTT‑GA‑TTGTTGTCTTCAACCGCCCTGAGAAACTCACT‑CTTCCCACAGCGCCAGTGCCAACGGTTTTTCATCATCCGTACGCCCGTTTACCCCCGGG‑CTGGACAAAAACTGCTTTAACaaat                                      >  2:799239/1‑147 (MQ=255)                                                                                                                    cGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTCTTCAAACGCCATGAGAAACTCACT‑TTGCCCCCAGCGCCAGTGCCAACGATTTATCATCATCCCTACGCTCCATTAACGCCGGG‑CTGGACAAAAACTGCTTTAACAATTc                                     >  1:37877/1‑149 (MQ=255)                                                                                                                       ta‑taTTTTTTTTTAAAACGGGGGT‑TTG‑AT‑TTTTTTTCTTCACCCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGt                                 <  1:1098967/150‑1 (MQ=255)                                                                                                                        ttatttttttttttAAACCGGGGG‑TTT‑AATTTTTTTTCTCCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAgg                                  <  2:1131376/146‑1 (MQ=255)                                                                                                                          tatttattGTTTAAACTGGCGG‑CTT‑AA‑ATGTTGTCTTCAAACGCCATGAGAAACTCACT‑CTTCCCCCAGCGCCCGTGCCAACGGTTTATTATCATCCCTACCCCCGTTTACCCCCGGGCCGGGACAAAAA‑TGCTTTTACAAATTAGGtaa                               >  2:688300/1‑150 (MQ=255)                                                                                                                            tttatgTTTTAAACGGGGGT‑TTA‑AA‑TTTTTGTCTCCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGt                                 <  1:567110/146‑1 (MQ=255)                                                                                                                             ttttttgttAAACCGGGGG‑TTT‑AATTTTTTGTCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGtaat                              <  1:979304/142‑1 (MQ=255)                                                                                                                             ttattGTTTAAACTGGCGG‑TTT‑AA‑TTGTTGTCTTTAACCGCCATGAGAAAATCACC‑CTGCCCACAGCGCCAGTGCCAACGGTTTATCATCATTCGTACGCTCGGTTACCGCCGGG‑CTGGACAAAAAATGCTTTAACAAATCAGGgaataa                            >  2:1191398/1‑150 (MQ=255)                                                                                                                                ttGTTTAAACTGGCGG‑TTT‑AA‑TTGTTGTCTTCAACCCCCCTGAGGAACTCACT‑CTGCCCACAGCGCCAGTGGCAACGTTTTATCATCATCCGTACGCCCGTTTACCGCCGGG‑CTGGACAAAAAATGCTTTAAAAATTTCGGTAATAAATc                         >  2:501629/1‑150 (MQ=255)                                                                                                                                ttGTTAAACCTGGCGG‑TTG‑AT‑TTTTTGTCTCCACCCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAAt                          <  2:875288/149‑1 (MQ=255)                                                                                                                                   ttaagccgggCTG‑TTG‑TT‑TTTTTGTCTACAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAg                      <  2:336412/142‑1 (MQ=255)                                                                                                                                      aaccggggtg‑TTA‑AA‑TTGTTGTCTACAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgtt                    <  2:942964/140‑1 (MQ=255)                                                                                                                                      aaaCTGGCGG‑TTG‑AT‑TTTTTGTCTTCACCCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAg                      <  2:951208/147‑1 (MQ=255)                                                                                                                                           ggggg‑TTG‑AA‑TTGTTGTCTCCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttgtt                 <  1:987052/144‑1 (MQ=255)                                                                                                                                           ggggg‑TTA‑AG‑TTGTGTTCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTg             <  2:1133473/148‑1 (MQ=255)                                                                                                                                              gg‑TTG‑AT‑TTGTTTTCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGt          <  1:976749/151‑1 (MQ=255)                                                                                                                                                   ttaatttgtttTCTTAACCCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGtt         <  2:349079/145‑1 (MQ=255)                                                                                                                                                   g‑AA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgct      >  1:344754/1‑151 (MQ=255)                                                                                                                                                      atttgtttTTTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGCCAAAAACTGCTTTAACAATTCAGGTAATAAATCGATTTGTTCCTGCGTtgct      <  2:1204948/148‑1 (MQ=255)                                                                                                                                                      a‑TTGTTGTCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgctgc    >  1:453174/1‑151 (MQ=255)                                                                                                                                                         tgttgtCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTgccg  >  1:1170847/1‑151 (MQ=255)                                                                                                                                                         tgttgtCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTgccg  >  1:749620/1‑151 (MQ=255)                                                                                                                                                          gttttCTTATACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCTTtgct      <  2:666277/146‑1 (MQ=255)                                                                                                                                                          |                                                                                                                                                        GATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAA‑TTAGTTCGCTAATCAGCCGTTA‑TATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACT‑CTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCG  >  NC_000913/2151538‑2151835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑