Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,903,6920CA43.6% 140.2 / 79.9 101intergenic (+318/+1055)ygcE/queEputative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base C (8/49);  new base A (43/1);  total (51/50)
Fisher's exact test for biased strand distribution p-value = 4.24e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 101 total aligned reads displayed.

GGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTA  >  NC_000913/2903552‑2903838
                                                                                                                                              |                                                                                                                                                          
ggAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑g                                                                                                                                                 <  1:240219/151‑1 (MQ=255)
   atatTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCt                                                                                                                                              <  1:674088/151‑1 (MQ=255)
             cacTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATTTAAAAACAATCAATA‑TCCTC‑aaaaa‑aaa                                                                                                                                   >  2:827913/1‑150 (MQ=255)
                tCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTTTTAAAACACCCATTT‑ACCTC‑aaaaa‑aaaaa                                                                                                                                 >  1:1172882/1‑147 (MQ=255)
                 cGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑Caa                                                                                                                             <  2:437375/151‑1 (MQ=255)
                 cGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑Caa                                                                                                                             <  2:226714/151‑1 (MQ=255)
                  gATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑Caaa                                                                                                                            <  2:329038/151‑1 (MQ=255)
                      tCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTTTAAAAACACCCATTA‑AACTC‑aaaaa‑aaaaa‑aaaaa                                                                                                                           >  1:975864/1‑147 (MQ=255)
                       cTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATTTAAAAACACTCATTA‑GCCTC‑aaaaa‑aaaaa‑aaaa                                                                                                                            >  1:977190/1‑146 (MQ=255)
                       cTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAAACACTCATTA‑ACCTC‑aaaaa‑aaaaa‑aaaa                                                                                                                            >  1:410730/1‑146 (MQ=255)
                         ttAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTcc                                                                                                                       <  1:13172/149‑1 (MQ=255)
                           aGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑CAATCTACAAACAACCCTTC‑GCCTC‑CAAAC‑CAAGA‑AAAACtctcac                                                                                                                     >  2:174109/1‑144 (MQ=255)
                                 aaaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTGTTAAAAAACACAATA‑TCCTC‑AAAAA‑AAAAA‑AAAACCTTCAA‑Atttt‑tt                                                                                                            >  2:125568/1‑150 (MQ=255)
                                  aaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATTTAAAAACACTCACTT‑TCCTC‑CAAAA‑AAaaa‑caaacattctcaattttt                                                                                                              >  1:432422/1‑136 (MQ=255)
                                    gACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACtt                                                                                                        <  1:943434/151‑1 (MQ=255)
                                      cAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTTTAAAAACACTCATTA‑AGCTC‑AAAAA‑AAAAA‑CAAACATCCAG‑Atttt‑tttttt                                                                                                        >  1:1136117/1‑145 (MQ=255)
                                      aaaTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATTTAAAAACAACCATTA‑TCCTC‑AAAAA‑AAAAA‑AAAAATTTCAG‑Attt                                                                                                                >  2:187570/2‑142 (MQ=255)
                                       aaTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAAc                                                                                                     <  1:119367/151‑1 (MQ=255)
                                          aCGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCaa                                                                                                  <  2:503469/151‑1 (MQ=255)
                                           cGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAAt                                                                                                 <  2:338652/151‑1 (MQ=255)
                                             aaTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTGTAAAAACACCACTTA‑AACTC‑AAAAA‑AAAAA‑AAAACATCCAC‑AATTT‑TTTCTTaa                                                                                                      >  1:880586/1‑144 (MQ=255)
                                             aaTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTGTAAAAACAATCCATA‑TGCTC‑AAAAA‑AAAAA‑AGAACTTCTAC‑ATTTATTTACTTAAcaa                                                                                                   >  1:503760/1‑146 (MQ=255)
                                             aaTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATTTAAAAAAAATCCTTA‑ACCTC‑AAAAC‑AAAAA‑CAAACATTCAG‑ATTTA‑TTAAtttatca                                                                                                   >  2:427009/1‑142 (MQ=255)
                                                cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATTTTAAAACACCCATTT‑ACCTC‑CAAAA‑AAAAA‑AAAAAATCCAG‑Atttt‑tttttttaacaataaa                                                                                              >  1:952854/1‑135 (MQ=255)
                                                  cACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGG‑TTAT‑TAAAACAAATCTTTT‑ATCCC‑AAAAC‑AAAAA‑GAAACTACCTG‑CCTTA‑TTAATTAAACacataat                                                                                             >  1:407358/1‑142 (MQ=255)
                                                  cACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTTTAAAAACAATCATTA‑TCCTC‑AAAAA‑AAAAA‑AAAACCTCCAG‑ATTTT‑TTACTTTAACaaataaaaa                                                                                           >  2:53892/1‑143 (MQ=255)
                                                   aCTTTATTAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTGTTAAAAAAAACATTATGCACC‑AAAAA‑AAAAA‑AAAACTTCCAA‑ATTTA‑‑TACATTAACAATGAaaaaa                                                                                          >  1:1144509/1‑149 (MQ=255)
                                                    cTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑CTATGTAAAACCAATCCTTA‑GGCCC‑CAAAA‑AAAGA‑AAAACCTCCAC‑ATTTT‑TTTCTTTACCaaaaaaaaa                                                                                           >  1:52989/1‑141 (MQ=255)
                                                       tATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCTTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGc                                                                                      <  1:1107102/150‑1 (MQ=255)
                                                        aTCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCtt                                                                                    <  1:827919/151‑1 (MQ=255)
                                                        aTCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCtt                                                                                    <  1:824104/151‑1 (MQ=255)
                                                          cAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTGTTAAAAAAATCAATA‑AACTC‑AAAAA‑AAAAA‑AAAACTTTCAA‑ATTTT‑TTATTTAAACaaaaaaaaaaaaaatta                                                                                   >  2:358546/1‑135 (MQ=255)
                                                           aaTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTGTAAAAACACTCATTA‑TCCTC‑CAAAA‑AAAAA‑AAAAATTCCAG‑ATTTA‑TTATTTAAACAAAAAAAAAAAAACTTaa                                                                                  >  1:286826/1‑150 (MQ=255)
                                                           aaTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTaaa                                                                                 <  2:602155/151‑1 (MQ=255)
                                                            aTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTaaaa                                                                                <  1:707515/151‑1 (MQ=255)
                                                              tttCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATTTTAAACTACACATTT‑AACTC‑AAAAA‑AAAAA‑AAAAAATCCAC‑ATTTA‑TTAATTAAACAAAAAATAAAAAACATaaaaa                                                                               >  2:1019739/1‑150 (MQ=255)
                                                                tCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAat                                                                             <  1:1152827/150‑1 (MQ=255)
                                                                    ttAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATATTAAAACAAACAATA‑ACATC‑AAAAC‑AAAAA‑AAAACATCCCA‑GTTTT‑TTATTTTATCAAAAAATAAAAAACTTcaaaaa                                                                              >  2:886083/1‑145 (MQ=255)
                                                                        aGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTa                                                                  <  2:372474/151‑1 (MQ=255)
                                                                         gTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTAt                                                                 <  1:582174/151‑1 (MQ=255)
                                                                          tATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTTTTAAAAAAACCATTA‑ACCTC‑AAAAA‑AAAGA‑AAAAAATTCAG‑ATTTT‑TTAATTTAACAAAAAAAAAAAAAACTAAAAAAAATAA‑TG‑TTAtt                                                                >  2:137201/1‑151 (MQ=255)
                                                                          tATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATATTAAAACACTCCTTT‑ATCCC‑CAAAC‑AAAAA‑CAAACCATCCC‑ATTTA‑TTACTTTACCAAAAAATAACAAACTTTAAAAAAATAC‑Ta‑ttatt                                                                >  2:261204/1‑151 (MQ=255)
                                                                           aTGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTTTTAAAACAATCATTA‑AACTCAAAAAA‑AAAAA‑AAAATTTCCAA‑ATTTT‑TAATTTAACAAAT‑AATAAAAAAATTAAAAAAAATAA‑TT‑TTAt                                                                 >  1:65952/1‑149 (MQ=255)
                                                                           aTGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTAtt                                                                <  1:1039385/150‑1 (MQ=255)
                                                                            tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAAACACCCATTA‑GCCTC‑AAAAA‑AAAGA‑AAAAATTCCAG‑ATTTT‑TTTTTTTAACAAAGAATAAAAAACTTTAAAAAAATAA‑TG‑Ttttttt                                                              >  1:807763/1‑149 (MQ=255)
                                                                             gAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTTTAAAAACACCCATTA‑ACCTC‑AAAAA‑AAAAA‑AAAAAATCCCG‑ATTTT‑TTATTTAAACAAAAAATAAAAAACTTAAAAAAAAAAA‑TT‑TTAtttt                                                              >  1:118926/1‑148 (MQ=255)
                                                                             gAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTGTAAAAACACCCATTT‑TCCTC‑ACAAC‑AAAAA‑AAAAATTTCCG‑ATTTT‑TTTTTTTAACAAATAAAAAAAAAATTAAAAAAAATAA‑TG‑Ttttt                                                                >  2:444369/1‑148 (MQ=255)
                                                                             gAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCt                                                              <  2:89069/150‑1 (MQ=255)
                                                                              aaCTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATTTAAAAACACCCAATA‑ACCTC‑CAAACAAAAAA‑AAAAATTCCA‑‑ATTTT‑TTATTTAAACAAAAAAAAAAAAACTTAAAAAAAATAA‑TG‑Ttttt                                                                >  1:14610/1‑147 (MQ=255)
                                                                              aaCTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTaa                                                            <  2:122615/151‑1 (MQ=255)
                                                                               aCTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTaaa                                                           <  2:560827/151‑1 (MQ=255)
                                                                                cTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTGTAAAAACAACCATTA‑TCCTC‑AAAAA‑AAAAA‑CAAACATTCCG‑AATTA‑TTAATTAAACAATGAATAAAAAAATTAAAAAAAATAA‑TG‑TTAtttt                                                              >  2:409355/1‑145 (MQ=255)
                                                                                 tGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTTTAAAAACACTCCTTA‑ACCTC‑AAAAA‑AAAAA‑AAAAAATCCAGAATTTT‑TTATTTAA‑CAATAAATAAAAAAATTAAAAAAAATAA‑TT‑TTAtttt                                                              >  1:607386/1‑144 (MQ=255)
                                                                                 tGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTGTAAAAACACCCATTT‑ACCTC‑CAAAA‑AAAAA‑AAAACATCCCG‑AATTT‑TTTATTTACCAAAGAAAAAAAAACCTaaaaaaaaaaattttttttt                                                                >  1:593133/1‑132 (MQ=255)
                                                                                 tGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTGTAAAAACACCCATTA‑ACCCC‑AAAAA‑AAAAA‑AAAAAATCCAG‑ATTTT‑TTATTTAACCCAAAAATAAAAAACTTAAAAAAAATAA‑TG‑TTAtttttaa                                                           >  1:219396/1‑144 (MQ=255)
                                                                                 tGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTGTTTTAAAACACACCTTA‑TTTTC‑AAAAC‑AAAAA‑AAAAAATCCCA‑ATTTA‑TTACTTAAACAAAAAATAAAAAAATTTAAAAAAATAC‑TG‑TTAtttttta                                                           >  2:1234717/1‑144 (MQ=255)
                                                                                  ggATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGa                                                         <  2:648503/150‑1 (MQ=255)
                                                                                  ggATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGa                                                         <  1:642308/150‑1 (MQ=255)
                                                                                          ttATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGT‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAAt                                                   <  1:290275/148‑1 (MQ=255)
                                                                                          ttATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGaaaaaa                                                    <  1:187558/147‑1 (MQ=255)
                                                                                          ttATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAAt                                                   <  2:642217/148‑1 (MQ=255)
                                                                                          ttATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAAAAACCCATTT‑AGCTC‑AAAAA‑AAAAA‑AAAACATCCCG‑ATTTA‑TTATTTTAACAATGAAAAAAAAAAATAAAAAAAAAAA‑TA‑TTTTTTTTAaaaaaaaaat                                                  >  1:502816/1‑147 (MQ=255)
                                                                                           taatCAGAAGAAGTATTCATCAACATCGTATAGAGGC‑GG‑TTATATAAAAACACGCATTA‑GCATC‑AAAAC‑AAAGA‑CAACCATCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGGTTAAAACATATAC‑TG‑GTATTCTAAATgaaaaa                                                    <  2:917178/143‑1 (MQ=255)
                                                                                           tATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GGTTTTTGAAAAACCCTCCATTA‑TCTCA‑AAAAC‑AAAAC‑CAAAATTCC‑G‑AATTT‑TTTGTTAAACAAACAATAACAACGCTAAAAAAAATACTTG‑TTTTTTAAAAGaaaaataaa                                                 >  1:701085/1‑146 (MQ=255)
                                                                                           tATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATTTAAAAAAACACAATT‑ACCCC‑AAAAC‑AAAAA‑AAAAAATCCAG‑ATTTA‑TTATTTAACCAATGAATAAAAAACATAAAAAAAAAAA‑TG‑TTATTTTTAAGAAAAAAATaa                                                >  2:73275/1‑150 (MQ=255)
                                                                                            aTTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACa                                              <  1:354150/151‑1 (MQ=255)
                                                                                            aTTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAAACACCCATTT‑ACCCC‑CAAAA‑AAAAA‑CAAAACTCCCA‑ATTTT‑TTATTTAAACAAAAAAAAAAAAAATTAAAAAAAATAA‑TT‑TTTTTTTAAAAAAAAAAATaa                                                >  1:40346/1‑149 (MQ=255)
                                                                                              tCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTTTAAAAACACCCATTA‑TCCTC‑AAAAA‑AAAAA‑AAAAAATTCAC‑ATTTT‑TTTATTTACCAAAGAAAAAAAAACTTAAAAAAAATAA‑TT‑TTTTTTTTAAAAAAAAAATaaaa                                              >  1:441156/1‑147 (MQ=255)
                                                                                              tCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAg                                             <  1:120577/150‑1 (MQ=255)
                                                                                               cATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTGTAAAAACAACCAATA‑TCCCC‑ACAAA‑AAAAA‑AAAACATCCCG‑AATTT‑TTAATTTAAAAAAAAAAAAAAAAACTAAAAAAAAAAA‑TG‑TTATTTTTAAAAAAAAATaaaaa                                              >  2:583273/1‑146 (MQ=255)
                                                                                               cATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTATAAAAACACTCCTTT‑GTCTC‑CAAAA‑AAAAA‑AAAACTTCCCG‑AATTA‑TTATTTAAACAATGAAAAAAAAGACTAAAAAAAAAAC‑TGTTTTTTTTAAAAAAAAAATTAAAAgg                                            >  1:305606/1‑151 (MQ=255)
                                                                                               cATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGc                                           <  1:795232/151‑1 (MQ=255)
                                                                                                   aGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTa                                       <  2:258516/151‑1 (MQ=255)
                                                                                                    gAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTTTGTAAAAACACCCATTT‑ACCTC‑AAAAA‑AAAAA‑AAAAATTTCAG‑ATTTT‑TTACTTAAACAAAGAATAAAAAACTTAAAAAAAATAA‑TG‑TTTTTTTTAAAAAAAAAATAAAAAGCATTa                                       >  1:196528/1‑150 (MQ=255)
                                                                                                     aaCTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAAt                                     <  2:1138651/151‑1 (MQ=255)
                                                                                                        tATTCATCAACAGCGTATAGAGGC‑GG‑TTTTTTAAAAACACCCAATT‑TCTTC‑AAAAC‑AAAAA‑CAAAATTTCCG‑ATTTT‑TTATTTTAAAAAACAATAAAAAACTTAAAAAAAAAAA‑TT‑TTATTTTTTAAAAAAAAATAAAAAGCAATaaat                                    >  2:113846/1‑147 (MQ=255)
                                                                                                         aTTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCa                                  <  1:372611/150‑1 (MQ=255)
                                                                                                          ttCATCAACAGCGTATAGAGGC‑GG‑TTATTTAAAAACACCCCTTA‑TACTC‑AAAAC‑AAAAA‑AAAAAATCCCG‑ATTTT‑TTTATTTAACAAAGAAAAAAAAACTTAAAAAAAAAAT‑TC‑TTTTTTTAAAAAAAAAATaaaaaacaataaattaa                                  >  2:1191511/1‑135 (MQ=255)
                                                                                                          ttCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAAACACCCATTA‑ACCTC‑AAAAA‑AAAAA‑AAAAATTCCCG‑AATTT‑TTAATTTAACAAAGAAAAAAAAACTTAAAAAATAAAA‑TG‑TTATTTTTAAAAAAAAAATAAAAAGCAATAATTTaa                                 >  1:715227/1‑150 (MQ=255)
                                                                                                             atcaACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATAtt                             <  2:613449/151‑1 (MQ=255)
                                                                                                              tcaACAGCGTATAGAGGC‑GG‑GTTTGTAAAAACACCCCTTA‑ACCCC‑CAAAA‑AAAAA‑AAAACCTTCCG‑ATTTT‑TTAATTAAACCACGAAAAAAAAACCTAAAAAAAAAAA‑TG‑TTTTTTTTAAAAAAAAAATAAAAAGAAATAATTTAATAtt                             >  2:311141/1‑150 (MQ=255)
                                                                                                                aaCAGCGTATAGAGGC‑GG‑TTATTTTAAAACAATCATTT‑TCCTA‑CAAAC‑AAAAACCAAATTTCCA‑‑ATTTA‑TTTACTAACACAAGAATAACAAACTTTAAAAAAAAAA‑TT‑TTTTTTTTTAGAAAAAAATAAAAAGCATTTATTTAATAttta                           >  2:982012/1‑148 (MQ=255)
                                                                                                                 aCAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTaaaa                         <  2:327094/151‑1 (MQ=255)
                                                                                                                     cGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaat                     <  2:1068867/151‑1 (MQ=255)
                                                                                                                        ataGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaataa                   <  2:788242/150‑1 (MQ=255)
                                                                                                                        ataGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaataa                   <  1:64934/150‑1 (MQ=255)
                                                                                                                           gagGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGa               <  2:948390/151‑1 (MQ=255)
                                                                                                                            agGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑TTTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGa               <  2:2759/150‑1 (MQ=255)
                                                                                                                            agGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGa               <  2:31783/150‑1 (MQ=255)
                                                                                                                              gC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGAtt             >  1:651881/1‑150 (MQ=255)
                                                                                                                              gC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTa            <  1:327880/151‑1 (MQ=255)
                                                                                                                               c‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaa           <  2:869398/151‑1 (MQ=255)
                                                                                                                                 gg‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaaa          <  2:17678/151‑1 (MQ=255)
                                                                                                                                  g‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaaaa         <  2:607362/151‑1 (MQ=255)
                                                                                                                                  g‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaaaa         <  2:593111/151‑1 (MQ=255)
                                                                                                                                  g‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaaa          >  1:1202531/1‑150 (MQ=255)
                                                                                                                                        gTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTc     <  1:427022/150‑1 (MQ=255)
                                                                                                                                        gTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCt    >  1:628810/1‑151 (MQ=255)
                                                                                                                                          aaaaCCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAGCAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTcat   <  2:958685/150‑3 (MQ=255)
                                                                                                                                          aaaaCCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTa  <  1:982275/151‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                          
GGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGC‑GG‑TTATGTAAAACCACTCATTA‑GCCTC‑AAAAC‑AAAGA‑CAAACTTCCAG‑ATTTA‑TTACTTAACCAATGAATAACAAGCTTAAAAAATATAC‑TG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTA  >  NC_000913/2903552‑2903838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑