Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	43.3%	63.6 / 53.0	67	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (6/32);  new base T (29/0);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 3.66e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206289‑3206583                                                                                                                                                    |                                                                                                                                                       gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGa                                                                                                                                                    <  2:506147/151‑1 (MQ=255) gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGa                                                                                                                                                    <  1:716732/151‑1 (MQ=255)   aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑a                                                                                                                                                 <  2:297040/151‑1 (MQ=255)      tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGAC‑aaa                                                                                                                                               >  1:340013/1‑150 (MQ=255)       cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAAt                                                                                                                                              <  2:989546/150‑1 (MQ=255)        gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATATTTTCAGAC‑aaa                                                                                                                                               >  2:424006/1‑148 (MQ=255)          aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAGTC‑aaa                                                                                                                                               >  2:1129882/1‑146 (MQ=255)                 tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTg                                                                                                                                    <  1:1047993/150‑1 (MQ=255)                   ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑g                                                                                                                                <  1:509901/151‑1 (MQ=255)                     aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑Gtt                                                                                                                              <  1:975415/151‑1 (MQ=255)                      ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAGAA‑AAAAAAAAAAAATGGA‑ggt                                                                                                                              >  2:139059/1‑148 (MQ=255)                         ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCa                                                                                                                          <  1:311888/151‑1 (MQ=255)                          cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTTAGAC‑AAAAAAAAAAAATTGA‑GTTggc                                                                                                                           >  2:1174567/1‑147 (MQ=255)                          cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAGAA‑AAAAAAAAAAAATTGG‑GTTGGCat                                                                                                                         >  2:1161088/1‑151 (MQ=255)                           tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGA‑GAATTTTTTCCGAC‑AAAAACAAAAAATAGA‑GTTGGCat                                                                                                                         >  2:36797/1‑150 (MQ=255)                               ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCatgat                                                                                                                      <  2:1045171/149‑1 (MQ=255)                                tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGC‑GAATATTTTTAGAA‑AAATATAAAAAATTGA‑Gtt                                                                                                                              >  2:773692/1‑140 (MQ=255)                                 ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCAtgatga                                                                                                                    <  1:996673/149‑1 (MQ=255)                                 ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAg                                                                                                                   <  1:1142541/150‑1 (MQ=255)                                   cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGa                                                                                                                <  2:156203/151‑1 (MQ=255)                                   cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGa                                                                                                                <  1:905623/151‑1 (MQ=255)                                                  tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCCA‑AC‑AAAAAAAAAAAATGGA‑GGTGCCATGATGAGCGGAAGGAAAAAACaa                                                                                                   >  1:1151174/1‑149 (MQ=255)                                                  tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCaa                                                                                                   <  1:432479/149‑1 (MQ=255)                                                   aTTTTCGCGTTGATTCGCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCa                                                                                                    <  2:459335/147‑1 (MQ=255)                                                   aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAg                                                                                                <  2:714477/151‑1 (MQ=255)                                                     tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAGAC‑AAAAACAAAAAATGGA‑GTTTGCATGATGAGCGAAAGTAAAAAACaaaaag                                                                                               >  1:623945/1‑146 (MQ=255)                                                       tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAGAC‑AAAAACAAAAAATGGG‑GTTTGCCTGATGAGGGAAAGtaataaaaaaaaag                                                                                               >  1:1237284/1‑140 (MQ=255)                                                       tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGAC‑AAAAACAAAAAATGGA‑GTTGGCATGATGAGCGAAAGAAAAAAAAAACa                                                                                                 >  1:671965/1‑146 (MQ=255)                                                       tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                             <  1:478102/150‑1 (MQ=255)                                                       tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                             <  1:910887/150‑1 (MQ=255)                                                              gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                                     <  1:771948/151‑1 (MQ=255)                                                                ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGAA‑AAAAAAAAAAAATGGA‑GTTGCCATGATGAGCGAAAAAAAAAAACAAAAGGCAGTGAaaaa                                                                                     >  1:402488/1‑149 (MQ=255)                                                                  cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑TTATTTTTTCAAAC‑AAATAAAAAAAACGGA‑GTTGTCATTGTGTTCGAAAGCAAAAACGAACAAGCGGGGAaaaa                                                                                     >  1:996696/1‑147 (MQ=255)                                                                  cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTg                                                                                 <  1:970902/151‑1 (MQ=255)                                                                   aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGa                                                                                <  1:1012457/151‑1 (MQ=255)                                                                          gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGAC‑AAAAACAAAAAATGGA‑GTTGCCATGATGAGCGAAAGTAATAAACAAAAGGCAGGGAAAAAAtt                                                                                  >  1:516393/1‑142 (MQ=255)                                                                           ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GAAATTTTTCAAAC‑AAATACAAAAAAAGGA‑GGTTCCATGGTGAGGGGAAGGAAAAAAAAAAAAGCAGGGGAAAAATTGACAGAGAt                                                                         >  2:55060/1‑150 (MQ=255)                                                                             aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTg                                                                       <  2:1112745/150‑1 (MQ=255)                                                                               ttACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAGAC‑AAAAAAAAAAAAAGGA‑GGTGCCATGATGAGCGAAAAGAATAAAAAACAAGCAGTGAAAAAATTTACAGAGATTGttg                                                                    >  1:936747/1‑149 (MQ=255)                                                                                tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg                                                                    <  1:401596/150‑1 (MQ=255)                                                                                tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg                                                                    <  1:451141/150‑1 (MQ=255)                                                                                        cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGAC‑AAAAAAAAAAAATGGA‑GTTGGCATGATGAGCGAAAGGAATAAGCAAAAGGGAGTGAATAAGTTGACAGAGATTGGCGCTAACttt                                                            >  1:265025/1‑150 (MQ=255)                                                                                        cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACttt                                                            <  1:433106/150‑1 (MQ=255)                                                                                         aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAAAA‑AAAAAAAAAAAATGGA‑GGTTGCATGTTGAGCGGAAATAAAAAACAAAACGCAGTGAAAAAAATTACAgagaattgcgccaaattt                                                            >  2:493590/1‑135 (MQ=255)                                                                                         aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCACAC‑AAAAACAAAAAATTGA‑GGTGCCAAGAGGTGAGAAAGTAAAAAACAACAGGGGGGGAATAAGTTTACAGAGAATGACGCTTAAttt                                                            >  1:1126938/1‑149 (MQ=255)                                                                                          cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAGACAAAAAAAAAAAACGGGG‑GTTGCAAGGAGGGGGGAAAGAAAAAAAAAAAAGGAAG‑GAAAAAGATGTGAGAGAGTGTGGCTCACttttac                                                         >  2:949588/1‑148 (MQ=255)                                                                                                 aaaaaaaaTAAACCCACATTTTTAGTGGTATTTTGTGGCGGT‑GAATTTTTCCAAAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAATTTCACAGGGATTGTCGTTACTTTTACCGCCATg                                                   <  2:15239/150‑1 (MQ=255)                                                                                                 aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGa                                                  <  2:471435/151‑1 (MQ=255)                                                                                                       tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAAC‑AAAAACAAAAACTGGA‑GGTGGCATGATGAGCGGAAATAAAAAAAAACAAGCAGGGGATAAAGTGACAGAGAATGTTGCTAACTTTAACCCCCTGAttttt                                             >  1:526782/1‑148 (MQ=255)                                                                                                        ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGAATTTTTCAAAA‑AAAAACAAAAAACTGA‑GTTTGCATGGAGAGGGAAAAGAATAAAAAAAAGGCAATGAATAAGTTGAAAGAGATTTGCGCTAACTTTACCGCCATGAttttt                                             >  2:366481/1‑147 (MQ=255)                                                                                                          aaaaCCCCATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAA‑AAATACAAAACCTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTAccc                                         <  1:1155034/151‑1 (MQ=255)                                                                                                          aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGAC‑AAAAACAAAAAATGGA‑GTTGCCATGATGGGCGAAAAGAATAAACAAAAAGCAGGGAATAAATTGACAGAGAATGTCGCTAACTTTTACGCCACGATTTTTAccc                                         >  1:481554/1‑151 (MQ=255)                                                                                                          aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAACTTTTTCTGAC‑AAAAACAAAAACTTGAGGTGGTCAT‑ATGAGCGAAAGTAATAAGCAACAAGCAGGGCATTAAATGACAGAGAATTTCGCCAACATTTCCTCCCTGtttt                                               >  2:873189/1‑145 (MQ=255)                                                                                                             aCCACATTTTTAGTGGTAGTTTGTGGCGGC‑GTATTTTTTCCGAC‑AAATACAAAAAATGGG‑GGTGCCCTTATGAGAGGAAGAAAAAAACAACAGGCAGTGAAAAAATTGACAGAGATTGTCGCTTACTTTTCCCCCCTGATTTTTAccc                                         >  1:719140/1‑148 (MQ=255)                                                                                                                    ttttAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCAAC‑AAATACAAAAAATGGA‑GTTGGCCTGATGAGGGAGAGGAATAAAAAACAGGCAGGGAATAAATTGAAAGAGATTGTGGCTAACTTTTCCCCCCAGATTTTTAACCCaaaggc                                  >  1:87915/1‑144 (MQ=255)                                                                                                                       tAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCGAC‑AAAAACAAAAACTTGG‑GTTGCCCTGATGAGCGAAAGGAATAAACAACAAGCAGGGAATAAGTTGACAGAGATTGTTGCTAACTTTACCGCCCTGATTTTTACCCCAATGcc                                  >  1:915978/1‑145 (MQ=255)                                                                                                                           ggTAGTTTGTGGCGGT‑GAATTTTTTCAGAC‑AAAAAAAAAAAATGGA‑GTTGCCATGATGAGCGAAAAGAATAAACAACAAGCAGTGAAAAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCCAAAGCCCGATGACggg                        >  1:393147/1‑149 (MQ=255)                                                                                                                           ggTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                        <  1:372971/151‑1 (MQ=255)                                                                                                                            gTAGTTTGTGGCGGT‑GAATTTTTTCCAAC‑AAATAAAAAAAACGGA‑GGTGGCATGATGAGCGAAAGAAATAAGAAACAGGCAGAGAATAAGTTGACAGAGAATGTCGCTAACTTTACCCCCATGATTTTTACCCGAATGCCTGAAGACgtgg                       >  2:186084/1‑151 (MQ=255)                                                                                                                                    gggCGGT‑AAATTTTTCAAAAC‑AAATACAAAAACTGGG‑GTTGCAATGAAGAGCAAAATTAATAACAACCAGGCTGTGAATAAGTTGACAAAGATTGTCGCAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGtata                 <  2:566579/148‑1 (MQ=255)                                                                                                                                             aaTTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGGGAAAGTAATAATCAACGGGGAGTGAAAAAGTAGACAGAGTTTGTGTCTAACTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa         <  1:228582/149‑1 (MQ=255)                                                                                                                                             aaTTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc        >  1:1079845/1‑150 (MQ=255)                                                                                                                                             aaTTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc        >  1:658622/1‑150 (MQ=255)                                                                                                                                              aTTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc        >  2:42574/1‑149 (MQ=255)                                                                                                                                               ttttcCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTGGACAGATATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAg      <  2:174269/150‑1 (MQ=255)                                                                                                                                                   tCCTGAC‑AAATACAAAAACTGGA‑GTTGCCATGAAGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGCGATTGTCGCTAACTTTACCGCCAAGATTTCTACCGGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc     >  2:288434/1‑147 (MQ=255)                                                                                                                                                   tCCCGAC‑AAATACACAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  >  2:18847/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                       GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAC‑AAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206289‑3206583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑