Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,903,155 C→T G183S (GGC→AGC)  bglB ← cryptic phospho‑beta‑glucosidase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,903,1550CT98.3% 225.3 / NA 60G183S (GGC→AGC) bglBcryptic phospho‑beta‑glucosidase B
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (27/32);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACCAGCCCACCGAGAAGCATATTGCCGATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCCCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTC  >  NC_000913/3903008‑3903300
                                                                                                                                                   |                                                                                                                                                 
aaaCCAGCCCACCGAGAAGCATATTGCCGATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCg                                                                                                                                                 <  2:852667/150‑1 (MQ=255)
 aaCCAGCCCACCGAGAAGCATATTGCCGATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTg                                                                                                                                               <  2:676775/151‑1 (MQ=255)
 aaCCAGCCCACCGAGAAGCATATTGCCGATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTg                                                                                                                                               <  2:614136/151‑1 (MQ=255)
  aCCAGCCCACCGAGAAGCATATTGCCGATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTg                                                                                                                                               >  1:1240354/1‑150 (MQ=255)
   ccAGCCCACCGAGAAGCATATTGCCGATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGaa                                                                                                                                             <  1:481704/151‑1 (MQ=255)
   ccAGCCCACCGAGAAGCATATTGCCGATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGaa                                                                                                                                             <  2:395943/151‑1 (MQ=255)
    cAGCCCACCGAGAAGCATATTGCCGATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGaa                                                                                                                                             >  2:1088915/1‑150 (MQ=255)
      gCCCACCGAGAAGCATATTGCCGATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATgg                                                                                                                                          <  1:774088/151‑1 (MQ=255)
      gCCCACCGAGAAGCATATTGCCGATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATg                                                                                                                                           >  2:1063289/1‑150 (MQ=255)
         cACCGAGAAGCATATTGCCGATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGgcgc                                                                                                                                       >  1:1015984/1‑151 (MQ=255)
           ccGAGAAGCATATTGCCGATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCgt                                                                                                                                     <  2:729422/151‑1 (MQ=255)
              agaAGCATATTGCCGATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCgtgt                                                                                                                                   >  1:496221/1‑150 (MQ=255)
                aaGCATATTGCCGATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAAc                                                                                                                                >  2:1170985/1‑151 (MQ=255)
                  gCATATTGCCGATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGa                                                                                                                              <  2:258210/151‑1 (MQ=255)
                  gCATATTGCCGATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGa                                                                                                                              <  2:21150/151‑1 (MQ=255)
                      atTGCCGATTTTCGCTTCGGGGAGCAGGTTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAGCTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATg                                                                                                                          <  2:93552/151‑1 (MQ=255)
                       ttGCCGATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATg                                                                                                                          <  2:182715/150‑1 (MQ=255)
                            gATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGAtt                                                                                                                    >  2:1239280/1‑151 (MQ=255)
                               tttCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGATGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTc                                                                                                                  <  2:463649/150‑1 (MQ=255)
                                  cGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCAtt                                                                                                               >  1:1034818/1‑150 (MQ=255)
                                  cGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTGCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTa                                                                                                              <  2:412647/151‑1 (MQ=255)
                                   gCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTa                                                                                                              >  1:453024/1‑150 (MQ=255)
                                      tCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAAc                                                                                                           <  2:453012/150‑1 (MQ=255)
                                      tCGGGAAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTaaa                                                                                                            <  2:669047/149‑1 (MQ=255)
                                       cGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACg                                                                                                          >  2:63314/1‑150 (MQ=255)
                                              cAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCAt                                                                                                  >  1:967292/1‑151 (MQ=255)
                                                ggCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATaa                                                                                                <  1:16532/151‑1 (MQ=255)
                                                 gCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAAc                                                                                               <  2:973797/151‑1 (MQ=255)
                                                  cTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACg                                                                                              >  2:551630/1‑151 (MQ=255)
                                                     tGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGcc                                                                                            >  2:273387/1‑150 (MQ=255)
                                                        cAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACtt                                                                                        >  2:1062356/1‑151 (MQ=255)
                                                               tAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGtt                                                                                  <  2:511952/150‑1 (MQ=255)
                                                                aaCTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTgg                                                                                <  1:1152695/151‑1 (MQ=255)
                                                                aaCTGCCCGCGCACTGGCAACCAGTTAATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTgg                                                                                <  1:140501/151‑1 (MQ=255)
                                                                  cTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGt                                                                               >  1:815712/1‑150 (MQ=255)
                                                                       cgcgCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAg                                                                         <  1:568008/151‑1 (MQ=255)
                                                                                 cAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGt                                                               <  1:512824/151‑1 (MQ=255)
                                                                                   aCCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGc                                                             <  1:90726/151‑1 (MQ=255)
                                                                                    ccAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGc                                                             <  2:1015985/150‑1 (MQ=255)
                                                                                    ccAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCg                                                            <  1:148391/151‑1 (MQ=255)
                                                                                             ggtggATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATg                                                   <  1:866553/151‑1 (MQ=255)
                                                                                                ggATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTc                                                <  2:899452/151‑1 (MQ=255)
                                                                                                       cTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTg                                          >  1:128553/1‑150 (MQ=255)
                                                                                                       cTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGAAAAGACCGTGCGGGCGTAATGCTCGAAGTg                                          >  1:31184/1‑150 (MQ=255)
                                                                                                            aaaCTTCCGCCTCGCCACTCTCTTCTGACAGCCCCACGCCCGGGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGa                                     >  2:736681/1‑150 (MQ=255)
                                                                                                                  ccGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGc                              <  2:1082390/151‑1 (MQ=255)
                                                                                                                   cGCCTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGc                              >  2:497863/1‑150 (MQ=255)
                                                                                                                     ccTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCg                           <  1:198360/151‑1 (MQ=255)
                                                                                                                      cTCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCg                           >  1:1192655/1‑150 (MQ=255)
                                                                                                                       tCGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGAt                         >  1:793580/1‑151 (MQ=255)
                                                                                                                        cGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGAtt                        <  2:929028/151‑1 (MQ=255)
                                                                                                                        cGCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGAt                         <  2:851033/150‑1 (MQ=255)
                                                                                                                         gCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCTCGTGTAACGTCATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGGGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGAtt                        >  1:553175/1‑150 (MQ=255)
                                                                                                                         gCCACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTa                       <  1:882561/151‑1 (MQ=255)
                                                                                                                           cACTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGCAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTAGc                     >  1:237796/1‑151 (MQ=255)
                                                                                                                            aCTCTCTTCTGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTAGcc                    >  2:170946/1‑151 (MQ=255)
                                                                                                                                tcttctGCCAGCCCCACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTAGCCCAAc                >  1:530709/1‑151 (MQ=255)
                                                                                                                                             ccACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTAGCCCAACCGCCGTAGttttt   >  1:70501/1‑151 (MQ=255)
                                                                                                                                             ccACGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTAGCCCAACCGCCGTAGttttt   >  2:1029851/1‑151 (MQ=255)
                                                                                                                                               aCGCTCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTc  >  2:674668/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AAACCAGCCCACCGAGAAGCATATTGCCGATTTTCGCTTCGGGGAGCAGGCTATGACAGGCTTTAACTGCCCGCGCACTGGCAACCAGTTGATGGTGGATAGCCTGATAAACTTCCGCCTCGCCACTCTCTTCTGCCAGCCCCACGCCCGTGAATGGCGCGTGTAACGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTC  >  NC_000913/3903008‑3903300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑