Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,106,405	T→C	intergenic (+85/‑64)	cpxP → / → fieF	inhibitor of the cpx response; periplasmic adaptor protein/ferrous iron and zinc transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,106,405	0	T	C	100.0%	278.5 / NA	74	intergenic (+85/‑64)	cpxP/fieF	inhibitor of the cpx response; periplasmic adaptor protein/ferrous iron and zinc transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/39);  total (35/39)

GACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTGATTAA  >  NC_000913/4106257‑4106548                                                                                                                                                     |                                                                                                                                                gacgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCtgccgc                                                                                                                                               <  2:1062403/151‑1 (MQ=255)   cgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCtt                                                                                                                                             <  2:935516/151‑1 (MQ=255)   cgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCtt                                                                                                                                             >  2:88394/1‑151 (MQ=255)      aaTGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAAt                                                                                                                                          <  2:278864/151‑1 (MQ=255)       aTGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATg                                                                                                                                         <  1:892029/151‑1 (MQ=255)            aaaaaaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTg                                                                                                                                    <  1:824410/151‑1 (MQ=255)             aaaaaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGc                                                                                                                                   >  1:542909/1‑151 (MQ=255)              aaaaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCa                                                                                                                                  <  2:225587/151‑1 (MQ=255)               aaaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGc                                                                                                                                   >  1:824334/1‑149 (MQ=255)                 aGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAgat                                                                                                                               <  1:617453/151‑1 (MQ=255)                    tCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAgatgat                                                                                                                            >  1:928235/1‑151 (MQ=255)                       tCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTg                                                                                                                         >  1:143967/1‑151 (MQ=255)                          ttGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCtt                                                                                                                      >  1:474601/1‑151 (MQ=255)                           tGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTc                                                                                                                     >  1:345783/1‑151 (MQ=255)                                 tATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTtat                                                                                                               >  1:612969/1‑151 (MQ=255)                                 tATTGAGCAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTCGACATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTta                                                                                                                >  2:1211190/1‑150 (MQ=255)                                  aTTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTtat                                                                                                               <  1:230629/150‑1 (MQ=255)                                  aTTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTtat                                                                                                               <  1:162337/150‑1 (MQ=255)                                  aTTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTtat                                                                                                               >  1:62204/1‑150 (MQ=255)                                  aTTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTtat                                                                                                               <  1:334804/150‑1 (MQ=255)                                  aTTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTta                                                                                                                <  1:300231/149‑1 (MQ=255)                                     gAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACt                                                                                                            <  1:986731/150‑1 (MQ=255)                                      agtagtAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACt                                                                                                            >  2:656688/1‑149 (MQ=255)                                      agtagtAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTa                                                                                                           <  2:327895/150‑1 (MQ=255)                                           tagCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTc                                                                                                      >  2:239758/1‑150 (MQ=255)                                            agCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCa                                                                                                     <  1:1064393/150‑1 (MQ=255)                                            agCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAg                                                                                                    <  2:759829/151‑1 (MQ=255)                                             gCAACTCACGTTCCCAGTTGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCACCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGCTTTGCTTCCGTTATACTAGCGTCAg                                                                                                    >  2:195699/1‑150 (MQ=255)                                               aaCTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTg                                                                                                 >  1:656249/1‑151 (MQ=255)                                                 cTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACCCCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGGTATACTAGCGTCAGttta                                                                                                <  2:598620/150‑3 (MQ=255)                                                    aCGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGc                                                                                            >  1:521230/1‑151 (MQ=255)                                                       ttCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCggg                                                                                         >  1:1186739/1‑151 (MQ=255)                                                        tCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCggg                                                                                         <  2:513046/150‑1 (MQ=255)                                                         cccAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCggg                                                                                         >  1:1181647/1‑149 (MQ=255)                                                         cccAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCggg                                                                                         >  1:693659/1‑149 (MQ=255)                                                         cccAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGa                                                                                        <  2:133140/150‑1 (MQ=255)                                                          ccAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGt                                                                                      >  2:599227/1‑151 (MQ=255)                                                           cAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGt                                                                                      >  1:10487/1‑150 (MQ=255)                                                           cAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTa                                                                                     <  1:567514/151‑1 (MQ=255)                                                            agtagtAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTAt                                                                                    <  2:582927/151‑1 (MQ=255)                                                                  aaaCCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATg                                                                               >  1:367573/1‑150 (MQ=255)                                                                  aaaCCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGa                                                                              <  1:600379/151‑1 (MQ=255)                                                                    aCCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGaat                                                                            <  1:378898/151‑1 (MQ=255)                                                                       cTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGaatca                                                                          >  1:792778/1‑150 (MQ=255)                                                                        tGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGaatcaat                                                                        >  1:292744/1‑151 (MQ=255)                                                                           tttCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCt                                                                      <  2:907028/150‑1 (MQ=255)                                                                            ttCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCtt                                                                     >  1:692546/1‑150 (MQ=255)                                                                              ccTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATg                                                                  <  2:878046/151‑1 (MQ=255)                                                                                  gCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACg                                                              >  1:179930/1‑151 (MQ=255)                                                                                       aGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTgg                                                         <  2:1079445/151‑1 (MQ=255)                                                                                        gACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGt                                                        >  1:1043321/1‑151 (MQ=255)                                                                                         acacCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTc                                                       >  1:650213/1‑151 (MQ=255)                                                                                              aTCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCg                                                  <  1:812161/151‑1 (MQ=255)                                                                                                    gTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGggcg                                              <  1:1211175/149‑1 (MQ=255)                                                                                                        tCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGgcggcg                                           <  1:330799/148‑1 (MQ=255)                                                                                                           cccACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATtgct                                     <  2:7832/151‑1 (MQ=255)                                                                                                           cccACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATtgct                                     >  1:691611/1‑151 (MQ=255)                                                                                                           accACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATtgct                                     >  2:1232292/2‑151 (MQ=255)                                                                                                                  gCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACgg                              <  1:499025/151‑1 (MQ=255)                                                                                                                  gCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACg                               <  2:47410/150‑1 (MQ=255)                                                                                                                        gtggTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTTCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGAt                          <  2:924/147‑1 (MQ=255)                                                                                                                             tttttttATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTc                    <  2:162748/150‑1 (MQ=255)                                                                                                                             tttttttATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTc                    <  1:776216/150‑1 (MQ=255)                                                                                                                              ttttttATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCgc                  >  1:66558/1‑151 (MQ=255)                                                                                                                              ttttttATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCgc                  >  2:319990/1‑151 (MQ=255)                                                                                                                                  ttATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCgctgct              >  2:707669/1‑151 (MQ=255)                                                                                                                                   tATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCgctgct              >  1:179097/1‑150 (MQ=255)                                                                                                                                    aTCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTa             <  1:862538/150‑1 (MQ=255)                                                                                                                                    aTCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTa             <  2:193118/150‑1 (MQ=255)                                                                                                                                    aTCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTAt            >  1:607316/1‑151 (MQ=255)                                                                                                                                    aTCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTAt            >  1:672368/1‑151 (MQ=255)                                                                                                                                      ccTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTAtt           <  2:897701/150‑1 (MQ=255)                                                                                                                                         cAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCt        <  2:928237/150‑1 (MQ=255)                                                                                                                                             ttGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTGAtt    <  2:961216/150‑1 (MQ=255)                                                                                                                                               gCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTGATTaa  <  1:1196688/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                GACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTGATTAA  >  NC_000913/4106257‑4106548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑