Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,114,622	A→G	S320P (TCC→CCC)	glpX ←	fructose 1,6‑bisphosphatase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,114,622	0	A	G	100.0%	280.9 / NA	72	S320P (TCC→CCC)	glpX	fructose 1,6‑bisphosphatase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/32);  total (40/32)

TTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTGCGCGCCATA  >  NC_000913/4114474‑4114767                                                                                                                                                     |                                                                                                                                                  tttttCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCt                                                                                                                                                 >  1:956160/1‑151 (MQ=255)  ttttCTCCTGTTGTGGGTGAATTGCCTTATCTTCGGTCTCCGAACAAAACAATTCCAGTACCGATGGACTGGAAAGCTCAATCGATCAAATCAATCAGAGGATGAGCAACTGCATTTCCGAGTCTTTGCGATCCAGATAGTGGATGGGCTg                                                                                                                                                >  2:912477/1‑151 (MQ=255)   tttCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGAATGGAAGGCTCAATCGATCAAATCAATCAGAGGATGCGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCt                                                                                                                                                 >  2:760528/1‑149 (MQ=255)    ttCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGa                                                                                                                                               <  2:809500/150‑1 (MQ=255)    ttCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGa                                                                                                                                               <  1:610553/150‑1 (MQ=255)    ttCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGa                                                                                                                                               <  2:567445/150‑1 (MQ=255)        ccTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAAt                                                                                                                                             <  1:884052/148‑1 (MQ=255)        ccTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATgc                                                                                                                                           <  2:220959/150‑1 (MQ=255)        ccTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATg                                                                                                                                            <  2:264635/149‑1 (MQ=255)           gTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATgcggcg                                                                                                                                       <  2:814400/151‑1 (MQ=255)               tgattgaCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATg                                                                                                                                   >  2:853996/1‑151 (MQ=255)                gattgaCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATgg                                                                                                                                  >  1:484544/1‑151 (MQ=255)                   tgaCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGc                                                                                                                               >  1:940992/1‑151 (MQ=255)                    gaCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGc                                                                                                                               <  1:642248/150‑1 (MQ=255)                     aCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCg                                                                                                                              >  1:40866/1‑150 (MQ=255)                     aCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGt                                                                                                                             <  2:35415/151‑1 (MQ=255)                          ccTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACtt                                                                                                                        >  1:549256/1‑151 (MQ=255)                                cTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCcgcg                                                                                                                  >  1:38454/1‑151 (MQ=255)                                  tCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCcgc                                                                                                                   <  2:583381/148‑1 (MQ=255)                                   cGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGAt                                                                                                               >  1:705014/1‑151 (MQ=255)                                      tctcCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATc                                                                                                              >  2:44512/1‑149 (MQ=255)                                      tctcCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATc                                                                                                              >  1:1004152/1‑149 (MQ=255)                                       ctcCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcagc                                                                                                           >  2:464400/1‑151 (MQ=255)                                         ccGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcagca                                                                                                          <  1:778539/150‑1 (MQ=255)                                         ccGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcagc                                                                                                           >  2:490670/1‑149 (MQ=255)                                         ccGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcagc                                                                                                           >  1:803294/1‑149 (MQ=255)                                         ccGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcag                                                                                                            <  2:141234/148‑1 (MQ=255)                                          agAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGAGGATGGGCTGAATGCGGCGCATGGTGCGTGACTTGCCGCGGATcagc                                                                                                           >  2:570778/2‑148 (MQ=255)                                                aaaaaaTTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCg                                                                                                  >  2:1109866/1‑151 (MQ=255)                                                        ccAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTcg                                                                                           <  2:303289/150‑1 (MQ=255)                                                          aGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTcgcg                                                                                         >  1:698548/1‑150 (MQ=255)                                                          aGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTcgcg                                                                                         >  2:584317/1‑150 (MQ=255)                                                          aGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGa                                                                                        <  1:158483/151‑1 (MQ=255)                                                             cccGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGatat                                                                                     <  2:692134/151‑1 (MQ=255)                                                             cccGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGata                                                                                      <  2:1126264/150‑1 (MQ=255)                                                                gAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGc                                                                                  >  1:153085/1‑151 (MQ=255)                                                                  aGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCt                                                                                >  2:587334/1‑151 (MQ=255)                                                                  aGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCt                                                                                <  1:100274/151‑1 (MQ=255)                                                                  aGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCt                                                                                >  1:729743/1‑151 (MQ=255)                                                                        ggAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCgg                                                                          <  1:627926/151‑1 (MQ=255)                                                                        ggAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCgg                                                                          <  2:596004/151‑1 (MQ=255)                                                                         gAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGAGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGc                                                                         >  2:586508/1‑151 (MQ=255)                                                                          aaGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCt                                                                        >  2:512639/1‑151 (MQ=255)                                                                                cAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGc                                                                   <  2:700552/150‑1 (MQ=255)                                                                                  aTCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCtt                                                                <  1:964209/151‑1 (MQ=255)                                                                                     gATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCca                                                             <  2:528251/151‑1 (MQ=255)                                                                                      atcaaatcaaTCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTcc                                                              >  2:609301/1‑149 (MQ=255)                                                                                          aatcaatcAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCcagc                                                           >  2:644252/1‑148 (MQ=255)                                                                                           atcaatcaGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCcagc                                                           >  1:723832/1‑147 (MQ=255)                                                                                              aatcaGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGc                                                    >  1:693090/1‑151 (MQ=255)                                                                                                tcaGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCt                                                  >  2:584688/1‑151 (MQ=255)                                                                                                 caGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCtt                                                 <  2:240932/151‑1 (MQ=255)                                                                                                 caGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCtt                                                 >  2:753809/1‑151 (MQ=255)                                                                                                    agGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTgg                                              >  1:718795/1‑151 (MQ=255)                                                                                                      gATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTa                                            <  1:874714/151‑1 (MQ=255)                                                                                                        tgtgCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAAt                                          >  2:1070125/1‑151 (MQ=255)                                                                                                         gtgCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAAt                                          <  2:1118384/150‑1 (MQ=255)                                                                                                           gCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATAcc                                       >  2:860495/1‑151 (MQ=255)                                                                                                               cTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGt                                    >  2:646606/1‑150 (MQ=255)                                                                                                                  cATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCa                                <  2:1232226/151‑1 (MQ=255)                                                                                                                   aTTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCag                               <  1:1191760/151‑1 (MQ=255)                                                                                                                     ttCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCaga                              <  2:565731/150‑1 (MQ=255)                                                                                                                     ttCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCaga                              <  1:760538/150‑1 (MQ=255)                                                                                                                      tCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCag                               >  1:1183813/1‑148 (MQ=255)                                                                                                                            tCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATg                       >  1:1222493/1‑150 (MQ=255)                                                                                                                                tGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGtt                  >  2:346347/1‑151 (MQ=255)                                                                                                                                  cGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTAt                >  1:686142/1‑151 (MQ=255)                                                                                                                                     tCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTACTACCGGTGGCAGAGAAGATGACGTTAt                >  2:165277/1‑148 (MQ=255)                                                                                                                                      ccAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGc             <  2:77099/150‑1 (MQ=255)                                                                                                                                       cAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTg           >  2:1228963/1‑151 (MQ=255)                                                                                                                                          aTAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTgcgc        <  1:544247/151‑1 (MQ=255)                                                                                                                                            aGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTgcgcgc      <  1:1000034/151‑1 (MQ=255)                                                                                                                                               ggatgggCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTGCGCGCCat   >  1:200329/1‑151 (MQ=255)                                                                                                                                                gatgggCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTGCGCGCCata  <  2:522071/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  TTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTGCGCGCCATA  >  NC_000913/4114474‑4114767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑