Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,128,316 A→G V27A (GTT→GCT)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,3160AG98.8% 320.7 / NA 84V27A (GTT→GCT) metJtranscriptional repressor, S‑adenosylmethionine‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (39/44);  minor base C (0/1);  total (39/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGT  >  NC_000913/4128168‑4128462
                                                                                                                                                    |                                                                                                                                                  
aTCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGTAATGGAAGcc                                                                                                                                                  <  2:927872/151‑1 (MQ=255)
 tCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCg                                                                                                                                                 >  1:591971/1‑151 (MQ=255)
  cGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCg                                                                                                                                                 <  2:161824/150‑1 (MQ=255)
  cGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGt                                                                                                                                                <  2:488732/151‑1 (MQ=255)
    gCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTa                                                                                                                                               >  2:270727/1‑150 (MQ=255)
     catcatCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAAt                                                                                                                                             <  2:402511/151‑1 (MQ=255)
     catcatCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAAt                                                                                                                                             >  1:497509/1‑151 (MQ=255)
     catcatCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAAt                                                                                                                                             <  1:426797/151‑1 (MQ=255)
         atcCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCttt                                                                                                                                         >  1:287154/1‑151 (MQ=255)
            cGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTg                                                                                                                                      <  2:478427/151‑1 (MQ=255)
               cAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACt                                                                                                                                   >  1:1041383/1‑151 (MQ=255)
                aaaGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACtt                                                                                                                                  >  2:694956/1‑151 (MQ=255)
                aaaGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACtt                                                                                                                                  >  1:1001188/1‑151 (MQ=255)
                    gTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTc                                                                                                                              <  1:1016443/151‑1 (MQ=255)
                         ccGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCActct                                                                                                                         >  1:318244/1‑151 (MQ=255)
                          cGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACtctt                                                                                                                        >  2:818829/1‑151 (MQ=255)
                           ggTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACtcttc                                                                                                                       <  2:740293/151‑1 (MQ=255)
                           ggTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACtcttc                                                                                                                       >  1:1093342/1‑151 (MQ=255)
                               aaGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGATGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGc                                                                                                                   >  1:898544/1‑151 (MQ=255)
                                aGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGcc                                                                                                                  >  2:505299/1‑151 (MQ=255)
                                         gAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAg                                                                                                          <  2:102423/150‑1 (MQ=255)
                                         gAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGc                                                                                                         <  1:751057/151‑1 (MQ=255)
                                              gCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATg                                                                                                    <  2:214783/151‑1 (MQ=255)
                                                ttCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATgg                                                                                                   >  1:965048/1‑150 (MQ=255)
                                                ttCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATgg                                                                                                   <  2:965046/150‑1 (MQ=255)
                                                 tCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATggg                                                                                                  <  2:952306/150‑1 (MQ=255)
                                                       agcagcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTGACTCTTCTTGCCGTGCTCAGCGTATGGGCTGatat                                                                                           <  1:263742/151‑1 (MQ=255)
                                                         cagcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGatatat                                                                                         <  2:1103938/151‑1 (MQ=255)
                                                          agcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATAtt                                                                                        <  2:414467/151‑1 (MQ=255)
                                                          agcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATAtt                                                                                        <  1:1173777/151‑1 (MQ=255)
                                                           gcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTc                                                                                       >  1:431053/1‑151 (MQ=255)
                                                           gcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTc                                                                                       >  2:475304/1‑151 (MQ=255)
                                                           gcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTc                                                                                       <  2:106702/151‑1 (MQ=255)
                                                              cGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTcgcc                                                                                    <  2:762245/151‑1 (MQ=255)
                                                              cGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTcg                                                                                      <  1:88412/149‑1 (MQ=255)
                                                                cTGTTGGGAGCGTGACGCGGGTTGTTAACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGATCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTagccgc                                                                                  >  2:1016416/1‑151 (MQ=255)
                                                                  gTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCt                                                                                 <  1:69050/150‑1 (MQ=255)
                                                                    tGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTcc                                                                               <  1:807102/150‑1 (MQ=255)
                                                                     ggTAGCGTGACGCAGGTTTTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCAt                                                                             <  2:480396/151‑1 (MQ=255)
                                                                     ggTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCa                                                                              <  1:1101372/150‑1 (MQ=255)
                                                                      gTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCAtt                                                                            <  2:268006/151‑1 (MQ=255)
                                                                         gCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTc                                                                           >  1:260362/1‑149 (MQ=255)
                                                                         gCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAg                                                                         <  2:588029/151‑1 (MQ=255)
                                                                             gACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCAt                                                                     <  1:1051455/151‑1 (MQ=255)
                                                                                  aGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCAt                                                                     >  2:44270/1‑146 (MQ=255)
                                                                                       gttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACtt                                                            <  2:1174564/150‑1 (MQ=255)
                                                                                         tCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTaa                                                          <  1:1114379/150‑1 (MQ=255)
                                                                                           aCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATcc                                                       >  2:1215380/1‑151 (MQ=255)
                                                                                               gacgacGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTTATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCctct                                                    <  2:1246204/150‑1 (MQ=255)
                                                                                               gacgacGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCctct                                                    <  1:62638/150‑1 (MQ=255)
                                                                                                  gacgCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGt                                                >  1:641614/1‑151 (MQ=255)
                                                                                                   acgCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTc                                               >  2:811047/1‑151 (MQ=255)
                                                                                                     gcgtgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCa                                              >  1:361897/1‑150 (MQ=255)
                                                                                                     gcgtgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCa                                              >  1:311652/1‑150 (MQ=255)
                                                                                                       gtgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATa                                           <  1:270720/151‑1 (MQ=255)
                                                                                                        tgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATa                                           >  2:570856/1‑150 (MQ=255)
                                                                                                         gcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATaa                                          <  2:1117853/150‑1 (MQ=255)
                                                                                                         gcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATaa                                          >  2:1035342/1‑150 (MQ=255)
                                                                                                         gcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATaa                                          >  2:1044691/1‑150 (MQ=255)
                                                                                                          cgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGCCTTGTTCACTCTTCTTGCCGTGCTCAGCGAATGGGCTGCTATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAAt                                        >  2:61670/1‑151 (MQ=255)
                                                                                                             tCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTg                                      <  1:114555/150‑1 (MQ=255)
                                                                                                                 cGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagacc                                 >  2:1042132/1‑151 (MQ=255)
                                                                                                                   gTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccag                               <  1:581743/151‑1 (MQ=255)
                                                                                                                     gAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccagac                             >  1:504637/1‑151 (MQ=255)
                                                                                                                      aGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccagacc                            >  2:464762/1‑151 (MQ=255)
                                                                                                                      aGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccagacc                            <  2:397287/151‑1 (MQ=255)
                                                                                                                        ttttttgAACACCTGGAGAGGATTGGAAGCCGTAATCTTTTGGACGTGTTCACTCTTCTTGCCGTGCTCAGCGTCTGGGCTGATATATTCGCCGCTCCATTCAGCCATGATATACTTAATCTTCTTCGTCACTAAATTGagatcagac                             >  2:958821/3‑148 (MQ=255)
                                                                                                                           ttttAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACcacac                        >  1:45899/1‑150 (MQ=255)
                                                                                                                            tttAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACcacac                        >  2:165381/1‑149 (MQ=255)
                                                                                                                              tAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGtt                    >  1:373424/1‑151 (MQ=255)
                                                                                                                               aaCACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGtt                    <  1:532050/150‑1 (MQ=255)
                                                                                                                                 cacCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAt                 <  2:712260/151‑1 (MQ=255)
                                                                                                                                  acCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAtg                >  2:1087294/1‑151 (MQ=255)
                                                                                                                                   ccTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAtg                <  2:1071384/150‑1 (MQ=255)
                                                                                                                                      tAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAtgtg              <  1:142800/149‑1 (MQ=255)
                                                                                                                                       aaGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTggg            <  2:109093/150‑1 (MQ=255)
                                                                                                                                       aaGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGt           >  1:846277/1‑151 (MQ=255)
                                                                                                                                         gagGAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTAc         <  2:341054/151‑1 (MQ=255)
                                                                                                                                          agGAATGGAACCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGCCCACACAGTTGATGTGGGTACt        <  2:761877/151‑1 (MQ=255)
                                                                                                                                            gAATGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTg       >  2:509667/1‑150 (MQ=255)
                                                                                                                                               tGGAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCGTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCg   >  1:698764/1‑151 (MQ=255)
                                                                                                                                                ggAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCg   <  2:898541/150‑1 (MQ=255)
                                                                                                                                                ggAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCg   <  2:1092301/150‑1 (MQ=255)
                                                                                                                                                ggAAGCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGt  >  1:874571/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGT  >  NC_000913/4128168‑4128462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑