Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	339,842	T→C	intergenic (+99/‑323)	yahG → / → yahI	DUF1116 family protein/carbamate kinase‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	339,842	0	T	C	100.0%	55.2 / NA	19	intergenic (+99/‑323)	yahG/yahI	DUF1116 family protein/carbamate kinase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/7);  total (12/7)

GCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAAT  >  NC_000913/339697‑339887                                                                                                                                                  |                                              gCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCAc                                            <  1:950407/149‑1 (MQ=255)   ttCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGccaccact                                        >  2:1244805/1‑149 (MQ=255)   ttCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAAGCCAGATAAGCGTAGCGCATCAGGCATTTGccaccact                                        >  2:785343/1‑149 (MQ=255)    tCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGccaccact                                        >  2:106214/1‑148 (MQ=255)     cGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGc                                      >  1:925652/1‑151 (MQ=255)      gagaAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGcc                                     >  2:97287/1‑151 (MQ=255)      gagaAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGcc                                     >  1:677117/1‑151 (MQ=255)       agaAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCg                                    <  1:982354/151‑1 (MQ=255)               aTTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATgcgg                             >  2:556408/1‑150 (MQ=255)                  cTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTg                         >  2:725569/1‑151 (MQ=255)                   ttGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTgg                        >  1:705652/1‑150 (MQ=255)                   ttGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTgg                        >  2:1104483/1‑150 (MQ=255)                    ttgCTGGTGCGAACGTTACGGATTCTGACCTACATGTTCATGCCGCATGCGGCGTGCACGCCTTATCAGGCCAACGAAAGGAGACAGATCGGGGAGGCCTGAAAAACGCAGAGCAAAAAGCACGAGCCACCACTGCCGGAtcctg                             >  2:812067/3‑141 (MQ=255)                    tGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTg                         <  2:1182060/149‑1 (MQ=255)                              gAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGGCGCCTTATcc             >  1:279653/1‑151 (MQ=255)                              gAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATcc             <  1:1097284/151‑1 (MQ=255)                               aaCGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACCCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGAAGCCTTATCCg            >  1:724032/1‑151 (MQ=255)                                 cGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGc          <  2:140789/151‑1 (MQ=255)                                  gTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAAAGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGAAGCCTTATCCCGcc         >  2:720678/1‑151 (MQ=14)                                    tACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCt        <  2:1145868/150‑1 (MQ=255)                                         cGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTGCCACCACTGCCGGGTGCGGCGTGGACGCCCTATCCGGCCTACGagg  >  2:309771/1‑149 (MQ=11)                                         cGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGagt  <  2:753742/151‑3 (MQ=255)                                                                                                                                                  |                                              GCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAAT  >  NC_000913/339697‑339887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑