Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	483,535	C→T	G290E (GGG→GAG)	acrB ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	483,535	0	C	T	100.0%	201.1 / NA	52	G290E (GGG→GAG)	acrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/29);  total (23/29)

GAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCA  >  NC_000913/483392‑483675                                                                                                                                                |                                                                                                                                             gAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGAcc                                                                                                                                       <  1:1215774/151‑1 (MQ=255)  aaTAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGAcct                                                                                                                                      <  2:209947/151‑2 (MQ=255)    tAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCgg                                                                                                                                     >  2:661996/1‑150 (MQ=255)     agagATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGaa                                                                                                                                   <  2:1103775/151‑1 (MQ=255)        gaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGcc                                                                                                                                <  2:460487/151‑1 (MQ=255)           tttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCgg                                                                                                                              <  2:375193/150‑1 (MQ=255)              cACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTgg                                                                                                                          <  2:776357/151‑1 (MQ=255)              cACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTgg                                                                                                                          >  1:352740/1‑151 (MQ=255)              cACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTg                                                                                                                           <  2:1061611/150‑1 (MQ=255)                 gAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCg                                                                                                                       >  2:759027/1‑151 (MQ=255)                   aCGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGtt                                                                                                                     <  1:531530/151‑1 (MQ=255)                     ggCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTaa                                                                                                                   <  2:1088959/151‑1 (MQ=255)                        gtggtgTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAAct                                                                                                                >  1:966583/1‑151 (MQ=255)                              tCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCg                                                                                                           >  2:928246/1‑150 (MQ=255)                               cGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCgat                                                                                                         <  1:800311/151‑1 (MQ=255)                                gTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCgatg                                                                                                        <  1:941423/151‑1 (MQ=255)                                       tAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGt                                                                                                 >  2:576315/1‑151 (MQ=255)                                        aaaCAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGt                                                                                                 >  2:65573/1‑150 (MQ=255)                                        aaaCAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGt                                                                                                 >  1:67427/1‑150 (MQ=255)                                        aaaCAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTa                                                                                                <  1:1036189/151‑1 (MQ=255)                                           cAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGGCCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGtt                                                                                             <  2:732717/151‑1 (MQ=255)                                            aaTTTTCAGACCCGACGGGAAGAACGGTTCCATGTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCTTTTGCACCGGTCGGCAGCTTGATCTCCAGTCCGCAGGCGGGGTCGCCGTTACCCTCCGCGACGCTGTcccaatt                                                                                             <  2:1138687/150‑7 (MQ=255)                                             aTTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTtct                                                                                           <  2:521978/151‑1 (MQ=255)                                              ttttCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTtctc                                                                                          >  1:92271/1‑151 (MQ=255)                                               tttCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTtctc                                                                                          <  2:1112266/150‑1 (MQ=255)                                                  cAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTcacc                                                                                       >  2:764326/1‑150 (MQ=255)                                                           cGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTc                                                                              <  1:707981/150‑1 (MQ=255)                                                            gggAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCaa                                                                            <  2:67775/151‑1 (MQ=255)                                                             ggAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAAt                                                                           >  2:938502/1‑151 (MQ=255)                                                              gaagaaCGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATc                                                                          <  2:55043/151‑1 (MQ=255)                                                                 gaaCGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTc                                                                       <  1:816278/151‑1 (MQ=255)                                                                   aCGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTcgc                                                                     >  2:154056/1‑151 (MQ=255)                                                                      gTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGAc                                                                  >  2:899999/1‑151 (MQ=255)                                                                         ccATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTc                                                               <  1:939817/151‑1 (MQ=255)                                                                             cTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACgc                                                           >  1:630360/1‑151 (MQ=255)                                                                               tCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACgca                                                          <  1:734608/150‑1 (MQ=255)                                                                                cGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACgcagc                                                        >  1:424033/1‑151 (MQ=255)                                                                                   cAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACgcagcagc                                                     <  2:135953/151‑1 (MQ=255)                                                                                        cAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCAcgc                                                 <  2:53037/150‑1 (MQ=255)                                                                                              ggATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAAcc                                          >  1:303938/1‑151 (MQ=255)                                                                                               gATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAAcc                                          >  1:662634/1‑150 (MQ=255)                                                                                                    ccGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTg                                    >  2:1202584/1‑151 (MQ=255)                                                                                                         gCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCa                               <  1:640857/151‑1 (MQ=255)                                                                                                               tCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTc                          >  1:307945/1‑150 (MQ=255)                                                                                                                      cgTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGAt                  >  2:858423/1‑151 (MQ=255)                                                                                                                       gTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGAtt                 >  1:466780/1‑151 (MQ=255)                                                                                                                           gCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTg              <  1:728949/150‑1 (MQ=255)                                                                                                                             aCCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCg           <  2:1073891/151‑1 (MQ=255)                                                                                                                               cGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCGCGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCg           >  1:926739/1‑149 (MQ=255)                                                                                                                                   cGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAActct     >  1:35071/1‑151 (MQ=255)                                                                                                                                    gCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCtt    <  2:206101/151‑1 (MQ=255)                                                                                                                                      cAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCa  <  2:1103474/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                             GAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCA  >  NC_000913/483392‑483675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑