Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 89,556 A→G intergenic (+524/‑78) cra → / → mraZ transcriptional repressor‑activator for carbon metabolism/RsmH methytransferase inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091389,5560AG98.5% 238.4 / ‑3.9 68intergenic (+524/‑78)cra/mraZtranscriptional repressor‑activator for carbon metabolism/RsmH methytransferase inhibitor
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (36/31);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-01

TTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTATCG  >  NC_000913/89410‑89701
                                                                                                                                                  |                                                                                                                                                  
tttAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCaa                                                                                                                                                 <  1:251763/150‑1 (MQ=255)
tttAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCa                                                                                                                                                  >  1:284624/1‑149 (MQ=255)
  tAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGc                                                                                                                                               <  1:244374/150‑1 (MQ=255)
    aaaCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCCAGCttt                                                                                                                                            >  1:1145444/1‑151 (MQ=255)
           gatgaCGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCa                                                                                                                                      <  2:790257/150‑1 (MQ=255)
           gatgaCGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAg                                                                                                                                     <  2:1141083/151‑1 (MQ=255)
                cGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCg                                                                                                                                >  1:724925/1‑151 (MQ=255)
                    gAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTaaa                                                                                                                            <  2:782134/151‑1 (MQ=255)
                              ttCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCCAGCTTTTCCTCCACTCCGTAAACTCCttt                                                                                                                     >  2:662008/1‑148 (MQ=255)
                               tCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTTCTCAGCTCCGTAAAATCCTTTCAGt                                                                                                                 >  2:707268/1‑151 (MQ=255)
                                   aCAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTggg                                                                                                              >  1:1038006/1‑150 (MQ=255)
                                     aGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTggg                                                                                                              >  2:784446/1‑148 (MQ=255)
                                      gCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAAt                                                                                                          <  1:822690/151‑1 (MQ=255)
                                       cTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAAtt                                                                                                         >  2:1101720/1‑151 (MQ=255)
                                          ttCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCCCCGTAAACTCCTTTCAGTGGGGAATtgtg                                                                                                      >  1:1216460/1‑151 (MQ=255)
                                           tCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAACCTCCTTTCAGTGGGAAATtgtg                                                                                                      <  2:217357/150‑1 (MQ=255)
                                            cATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTggg                                                                                                    <  1:831276/151‑1 (MQ=255)
                                            cATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTggg                                                                                                    >  1:47431/1‑151 (MQ=255)
                                               aaCGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTTAACTCCTTTCCGTGGGAAATTgggggg                                                                                                   >  1:835584/1‑149 (MQ=255)
                                               aaCGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGc                                                                                                 <  2:925485/150‑1 (MQ=255)
                                                aCGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTgggg                                                                                                   >  1:238213/1‑148 (MQ=255)
                                                aCGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAAATCCTTTCAGTGGGAAATTGTGGGGCa                                                                                                >  1:773462/1‑150 (MQ=255)
                                                 cGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCaaa                                                                                              <  1:103277/151‑1 (MQ=255)
                                                   ttAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGACTTGTGACTGGCTTGGGAAGCTTTTCCTCCGCCCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCaaa                                                                                              >  2:47969/1‑149 (MQ=255)
                                                   ttAATTGGCTTCGCACGTTGGACGTAAAATAAACAGCGCTGATATTAGCCGAAAGCATCGGGTTTTTTACCTCGGTATGCCTTGTGACTAGCTTGGCAAGCTTTTCCGCAGCTCCGTAAACTCCTTTCAGAGGGAAATTTTAGGGCaaaa                                                                                             <  2:646727/150‑2 (MQ=255)
                                                     aaTTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAgggg                                                                                          >  1:56940/1‑151 (MQ=255)
                                                     aaTTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTgg                                                                                          <  1:525382/151‑1 (MQ=255)
                                                        ttGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTggg                                                                                         >  1:759005/1‑149 (MQ=255)
                                                        ttGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTggg                                                                                         >  2:527485/1‑149 (MQ=255)
                                                        ttGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGa                                                                                        <  1:988136/150‑1 (MQ=255)
                                                         tGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGGAATTGTGGGGCAAAGTGGGAAt                                                                                      >  2:239481/1‑151 (MQ=255)
                                                           cTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGGAATTGTGGGGCAAAGTGGGAAt                                                                                      >  1:990643/1‑149 (MQ=255)
                                                                   gTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAg                                                                            <  1:785803/151‑1 (MQ=255)
                                                                      ggACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCCAGCTTTTCCCCAGCTCCGTAAACTCCCTTTCGTGGGGAAGTGTGGGGCCAAGGTGGGAGAAAgggggagg                                                                            >  2:170076/1‑145 (MQ=255)
                                                                      ggACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCt                                                                         <  1:1180703/151‑1 (MQ=255)
                                                                      ggACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGGGAGGCt                                                                         >  2:966192/1‑151 (MQ=255)
                                                                      ggACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGGGGGAATAAGGGGTGAGGc                                                                          >  1:142331/1‑150 (MQ=255)
                                                                        aCGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGGGGGGATAAGGGGTGAGGCTgg                                                                       >  2:286661/1‑151 (MQ=255)
                                                                        aCGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGATTTTACTCAGCTCCGGAAAATCCTTTCAGTGGGAAATTGTGGGGTAACGTGGGAATAAGGGGGGAggtt                                                                         >  1:446430/1‑147 (MQ=255)
                                                                          gTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCa                                                                     <  2:42231/151‑1 (MQ=255)
                                                                            aaaaTAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCCGTGGGGAATTGTGGGGCAAAGTGGGAATAAAGGGGGAGGCTGGcct                                                                    >  1:957118/1‑148 (MQ=255)
                                                                            aaaaTAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCAt                                                                    <  2:48094/150‑1 (MQ=255)
                                                                                  aacaacGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGGAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCgg                                                             >  2:420867/1‑151 (MQ=255)
                                                                                  aacaacGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCgg                                                             <  2:53188/151‑1 (MQ=255)
                                                                                  aacaacGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTAGCAAGGTTTTCCTCAGCCCCGTAAAATCCTTTCAGGGGGAAATTGTGGGGCAAAGTGGGGATAAGGGGTGAAGCTGTCATTTTcctg                                                             >  2:684214/1‑149 (MQ=255)
                                                                                    caacGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCgggg                                                           <  1:1180944/151‑1 (MQ=255)
                                                                                           gATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCaa                                                      <  2:835578/149‑1 (MQ=255)
                                                                                              atTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTa                                                 <  1:816643/151‑1 (MQ=255)
                                                                                               ttAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTa                                                 <  2:626098/150‑1 (MQ=255)
                                                                                               ttAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAACGTTTCCTCAGGTCCGAAAACTCCTTTCAGTGGGCAATTGTGGGGCCAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGTGCAACGtt                                                  >  1:410910/1‑149 (MQ=255)
                                                                                                 aGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGt                                               <  1:143028/150‑1 (MQ=255)
                                                                                                  gCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAAATCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTc                                              >  1:134734/1‑150 (MQ=255)
                                                                                                       aaaCATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGGAATTGTGGGGCAAAGGGGGGATAAGGGGGGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAAtctc                                        >  2:224423/1‑151 (MQ=255)
                                                                                                         aCATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGa                                      <  1:1191576/151‑1 (MQ=255)
                                                                                                          cATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGa                                      >  1:1023405/1‑150 (MQ=255)
                                                                                                          cATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGGGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGGGAGGCTGGCATGTTCCGGGGGGCAACGTTAGTCAATCTCg                                       >  2:996550/1‑149 (MQ=255)
                                                                                                                  tttttACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAg                              <  1:115455/150‑1 (MQ=255)
                                                                                                                           cGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCCGCGGGGAATTTTGGGGCAAAGAGGGGATAAGGGGTGAGGCTGGCATGTTCCGGGGGGCAACGTTAGTCAATTTCGACAGCAAAGGGCGCTTATc                    >  1:435676/1‑151 (MQ=255)
                                                                                                                           cGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTAt                     <  2:296701/150‑1 (MQ=255)
                                                                                                                           cGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATc                    <  1:799552/151‑1 (MQ=255)
                                                                                                                               aTGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGt                 <  1:618071/150‑1 (MQ=255)
                                                                                                                                     tgtgACTGGCTTGGGAAGCTTTTCCTCAGCTCCGGAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTTAATATCGACAGCAAAGGGCGCTTATCAGGGCCTAcc          >  2:1156254/1‑151 (MQ=255)
                                                                                                                                         aCTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGtt      <  1:848457/151‑1 (MQ=255)
                                                                                                                                          cTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGtt      >  2:63259/1‑150 (MQ=255)
                                                                                                                                            ggCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTa     <  1:967088/149‑1 (MQ=255)
                                                                                                                                            ggCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTAt    <  2:421354/150‑1 (MQ=255)
                                                                                                                                            ggCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTATc   >  2:1027310/1‑151 (MQ=255)
                                                                                                                                             gCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTATCg  <  2:1145467/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTATCG  >  NC_000913/89410‑89701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: