Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 736,382 T→C intergenic (+163/‑63) ybfC → / → ybfQ putative secreted protein/pseudogene, H repeat‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913736,3820TC99.0% 369.4 / NA 97intergenic (+163/‑63)ybfC/ybfQputative secreted protein/pseudogene, H repeat‑associated protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (45/51);  minor base A (0/1);  total (45/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGTCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTGTCAGAT  >  NC_000913/736234‑736528
                                                                                                                                                    |                                                                                                                                                  
aaaaCAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCt                                                                                                                                                  >  2:1187119/1‑151 (MQ=255)
aaaaCAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCt                                                                                                                                                  >  1:255350/1‑151 (MQ=255)
  aaCAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTaa                                                                                                                                                <  1:748183/151‑1 (MQ=255)
   aCAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTaaa                                                                                                                                               <  2:443833/151‑1 (MQ=255)
    cAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAg                                                                                                                                              <  1:524314/151‑1 (MQ=255)
     aaaTTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGc                                                                                                                                             <  1:914845/151‑1 (MQ=255)
     aaaTTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGc                                                                                                                                             >  1:314282/1‑151 (MQ=255)
         tAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGtt                                                                                                                                         >  2:881172/1‑151 (MQ=255)
         tAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGt                                                                                                                                          <  1:862953/150‑1 (MQ=255)
          aaTCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGttt                                                                                                                                        >  1:511868/1‑151 (MQ=255)
            tCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGttttt                                                                                                                                      >  2:1195425/1‑151 (MQ=255)
             cATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGTGAAAGATCACGTAACGCTTCTTTTTTGTACTAAATAATTCGAATTTTAAGTTGAACATTTGAGATCCTACCTATTGCCTCAAGCTGCTGTTc                                                                                                                                     <  2:657254/151‑1 (MQ=37)
             cATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTc                                                                                                                                     >  2:267740/1‑151 (MQ=255)
              aTTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTc                                                                                                                                     >  1:184157/1‑150 (MQ=255)
                  tgAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGc                                                                                                                                >  1:248519/1‑151 (MQ=255)
                    aaaaaTTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCtt                                                                                                                              <  1:75825/151‑1 (MQ=255)
                       aaTTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTtata                                                                                                                           <  1:823229/151‑1 (MQ=255)
                           atataACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATg                                                                                                                       <  1:203725/151‑1 (MQ=255)
                            tataACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATg                                                                                                                       >  2:30411/1‑150 (MQ=255)
                             ataACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGAt                                                                                                                     <  1:956194/151‑1 (MQ=255)
                             ataACGCATGGAATCAGTTATTATTTTGCGTTCCATTTGCAGGGATATATGACGTAACGCTACTTTTTTGTTCTAAGTAATTCGAATTTTAAGGTCAACAATTGGGATACTACTTATTCACTAAAGCTGTTTTTCATAGCTTATACAtttt                                                                                                                     <  2:822495/151‑4 (MQ=18)
                               aaCTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATc                                                                                                                    >  2:706844/1‑150 (MQ=255)
                               aaCTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCa                                                                                                                   <  1:192028/151‑1 (MQ=255)
                                aCTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCa                                                                                                                   >  1:10714/1‑150 (MQ=255)
                                 cTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCaa                                                                                                                  >  1:148764/1‑150 (MQ=255)
                                   cATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAAt                                                                                                                <  2:405437/150‑1 (MQ=255)
                                    aTGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATAc                                                                                                              >  2:764073/1‑151 (MQ=255)
                                      ggAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACt                                                                                                             <  1:1101978/150‑1 (MQ=255)
                                      ggAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACt                                                                                                             <  1:37639/150‑1 (MQ=255)
                                       gAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTcc                                                                                                           >  2:362176/1‑151 (MQ=255)
                                       gAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTcc                                                                                                           >  1:1155856/1‑151 (MQ=255)
                                           aGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTa                                                                                                        <  2:445000/150‑1 (MQ=255)
                                           aGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTa                                                                                                        >  2:1220754/1‑150 (MQ=255)
                                           aGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTa                                                                                                        <  1:925598/150‑1 (MQ=255)
                                           aGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTAc                                                                                                       >  1:950978/1‑151 (MQ=255)
                                             gtgtTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACat                                                                                                     >  1:70732/1‑151 (MQ=255)
                                               gtTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACata                                                                                                    <  1:270246/150‑1 (MQ=255)
                                                 ttATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACataaa                                                                                                  <  1:818959/150‑1 (MQ=255)
                                                  tATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACataaat                                                                                                 >  1:41493/1‑150 (MQ=255)
                                                   aTTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACataaata                                                                                                >  2:676749/1‑150 (MQ=255)
                                                    ttttACATTCGATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACataaataa                                                                                               <  2:255358/150‑1 (MQ=11)
                                                       tGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTg                                                                                           >  1:247590/1‑151 (MQ=255)
                                                         cGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGaa                                                                                         <  1:972477/151‑1 (MQ=255)
                                                           ttCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAAc                                                                                        >  1:11237/1‑150 (MQ=255)
                                                            tCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACa                                                                                       <  2:32310/150‑1 (MQ=255)
                                                               aTTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATg                                                                                   <  2:723078/151‑1 (MQ=255)
                                                                  tGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGa                                                                                 <  1:846770/150‑1 (MQ=255)
                                                                   gCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGaa                                                                                <  2:1097220/150‑1 (MQ=255)
                                                                          aaGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTaaaa                                                                         >  1:12522/1‑150 (MQ=255)
                                                                           aGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTaaaaaa                                                                       <  2:1244926/151‑1 (MQ=255)
                                                                           aGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTaaaaaa                                                                       <  1:997192/151‑1 (MQ=255)
                                                                             aTCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAAt                                                                      <  2:143110/150‑1 (MQ=255)
                                                                                    aaCGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGaa                                                              <  2:307529/151‑1 (MQ=255)
                                                                                    aaCGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGa                                                               >  2:153267/1‑150 (MQ=255)
                                                                                     aCGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAAc                                                             <  2:955270/151‑1 (MQ=255)
                                                                                     aCGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAAc                                                             >  1:718864/1‑151 (MQ=255)
                                                                                      cGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAAc                                                             >  1:553247/1‑150 (MQ=255)
                                                                                      cGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACa                                                            <  2:108669/151‑1 (MQ=255)
                                                                                      cGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACa                                                            <  1:359447/151‑1 (MQ=255)
                                                                                       gCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACat                                                           <  1:217192/151‑1 (MQ=255)
                                                                                        cTACTTTTCTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACat                                                           <  2:871712/150‑1 (MQ=255)
                                                                                          aCTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATAtt                                                        >  1:1018529/1‑151 (MQ=255)
                                                                                             tttttGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTc                                                      <  2:452012/150‑1 (MQ=255)
                                                                                              ttttGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCt                                                     >  2:206625/1‑150 (MQ=255)
                                                                                               tttGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTAt                                                   >  2:530323/1‑151 (MQ=255)
                                                                                                ttGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTAtt                                                  >  2:354084/1‑151 (MQ=255)
                                                                                                ttGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTAtt                                                  >  2:102307/1‑151 (MQ=255)
                                                                                                ttGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTAt                                                   <  2:153801/150‑1 (MQ=255)
                                                                                                  gTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTac                                                <  1:475363/151‑1 (MQ=255)
                                                                                                    aCTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTacac                                              >  2:1188384/1‑151 (MQ=255)
                                                                                                       aaaTAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCg                                           <  2:827582/151‑1 (MQ=255)
                                                                                                         ataatTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGAt                                         <  2:723842/151‑1 (MQ=255)
                                                                                                          taatTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGAtt                                        >  2:483981/1‑151 (MQ=255)
                                                                                                           aatTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGAtt                                        <  2:1011410/150‑1 (MQ=255)
                                                                                                           aatTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGAtt                                        >  2:564267/1‑150 (MQ=255)
                                                                                                            atTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTAc                                      <  1:723666/151‑1 (MQ=255)
                                                                                                             ttCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTAc                                      >  2:168069/1‑150 (MQ=255)
                                                                                                                gAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGAc                                  <  1:695948/151‑1 (MQ=255)
                                                                                                                gAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGAc                                  >  1:90072/1‑150 (MQ=255)
                                                                                                                 aaTTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAAGAAGGTGAACAAATCGAACTTAAAAAATTGATGGAACATATTTCTATTACGCCCGATTACAGACa                                 <  2:906049/151‑1 (MQ=255)
                                                                                                                  aTTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTAAATAAATAAGTTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACAACCGATTACAGACa                                 >  2:272704/1‑150 (MQ=255)
                                                                                                                   ttttAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACa                                 >  1:1216103/1‑149 (MQ=255)
                                                                                                                          ttCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGa                         >  1:381981/1‑150 (MQ=255)
                                                                                                                             aacaaTTGAGATACTACCTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAg                      >  1:120159/1‑150 (MQ=255)
                                                                                                                              acaaTTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAgtg                    <  2:8067/151‑1 (MQ=255)
                                                                                                                                 aTTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAgtggtg                 <  2:664763/151‑1 (MQ=255)
                                                                                                                                   tGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGc                <  2:165283/150‑1 (MQ=255)
                                                                                                                                     agaTACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATa             <  2:732344/151‑1 (MQ=255)
                                                                                                                                       aTACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATaaa           <  2:832921/151‑1 (MQ=255)
                                                                                                                                         actactTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAAtt         <  2:950976/151‑1 (MQ=255)
                                                                                                                                            actTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTg        <  2:841022/149‑1 (MQ=255)
                                                                                                                                            actTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTg        <  2:480038/149‑1 (MQ=255)
                                                                                                                                            actTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTGTc      >  2:434241/1‑151 (MQ=255)
                                                                                                                                            actTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTGTc      >  2:578024/1‑151 (MQ=255)
                                                                                                                                                aTTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTGTc      >  1:439858/1‑147 (MQ=255)
                                                                                                                                                aTTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTGTc      >  1:404401/1‑147 (MQ=255)
                                                                                                                                                aTTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTGTCAGat  <  2:504636/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGTCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTGTCAGAT  >  NC_000913/736234‑736528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: