Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	132,486	C→T	A291V (GCA→GTA)	acnB →	aconitate hydratase 2; aconitase B; 2‑methyl‑cis‑aconitate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	132,486	0	C	T	100.0%	231.4 / NA	69	A291V (GCA→GTA)	acnB	aconitate hydratase 2; aconitase B; 2‑methyl‑cis‑aconitate hydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/30);  total (39/30)

GGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGC  >  NC_000913/132340‑132632                                                                                                                                                   |                                                                                                                                                   gggTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACt                                                                                                                                                 >  1:1169007/1‑150 (MQ=255)  ggTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTg                                                                                                                                                >  1:590296/1‑150 (MQ=255)      cttcGCGTAAATCCGACACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTCAAAATGCACCCATCTTCCTTAACACGCTGGAAGACCCGGgcgctttcccga                                                                                                                                            >  2:382113/1‑142 (MQ=255)       ttcGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGGAGACGCGGGTGTACTGCCAATc                                                                                                                                          >  1:1238537/1‑151 (MQ=255)         cgcgTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGGGTACTGCCAATCg                                                                                                                                         >  1:49648/1‑150 (MQ=255)         cgcgTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGGGTAATGCCAAttg                                                                                                                                         >  2:871450/1‑148 (MQ=255)          tcgTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATc                                                                                                                                          <  2:221972/147‑1 (MQ=255)           cgTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGGGTAATGCCAATCg                                                                                                                                         >  2:58357/1‑148 (MQ=255)               aaTCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGa                                                                                                                                  <  2:32295/151‑1 (MQ=255)                      cACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGGGTACTGCCAATCGAAGTTCGCGtctc                                                                                                                            >  2:30138/1‑150 (MQ=255)                         tAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTaa                                                                                                                         >  1:889312/1‑150 (MQ=255)                          aaCTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTaa                                                                                                                         <  2:730627/149‑1 (MQ=255)                           aCTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCCTCTTCTTTAACCCGGTGGAAGAAGCGGGTGTAATGCCAATCGCAGTCGACGTCTCTAACCt                                                                                                                      >  1:1059911/1‑151 (MQ=255)                           aCTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCt                                                                                                                      <  1:1016632/151‑1 (MQ=255)                             tCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGGGTACTGCCAATCGAAGTCGACGGCTCTTAcc                                                                                                                       >  2:472490/1‑148 (MQ=255)                                  tCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATg                                                                                                               >  1:9883/1‑151 (MQ=255)                                             tGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGGGTACTGCCAATCGAAGTCGACGTCTCTAAACTGAACATGGGCGACGTGAt                                                                                                    >  1:176969/1‑151 (MQ=255)                                                gCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGa                                                                                                 <  2:973308/151‑1 (MQ=255)                                                  gatgatATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACg                                                                                               <  2:246326/151‑1 (MQ=255)                                                      atatTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGGCGTCTCTAACCTTAACATGGGCGACGGGATTGACGttt                                                                                            >  1:833876/1‑150 (MQ=255)                                                               aTGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGGGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACa                                                                                   >  1:967418/1‑150 (MQ=255)                                                                    ccGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGGGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACCTGATTGACGTTTACCCGTACAAAGGTg                                                                             >  1:783048/1‑151 (MQ=255)                                                                      gAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGa                                                                            >  1:72239/1‑150 (MQ=255)                                                                       aaCAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGa                                                                            >  1:938850/1‑149 (MQ=255)                                                                       aaCAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAg                                                                          <  1:505605/151‑1 (MQ=255)                                                                           aaCGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCg                                                                      <  2:630529/151‑1 (MQ=255)                                                                           aaCGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGGGTACTGCCAATCGAAGTCGACGTCTCTAAACTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGGGAAGTGCg                                                                      >  1:443523/1‑151 (MQ=255)                                                                                ggcggTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc                                                                 <  1:650408/151‑1 (MQ=255)                                                                                ggcggTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGGGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGGGAAGTGCGTAAcc                                                                 >  1:507785/1‑151 (MQ=255)                                                                                  cggTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCa                                                                >  1:288549/1‑150 (MQ=255)                                                                                  cggTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCAc                                                               <  1:408796/151‑1 (MQ=255)                                                                                   ggtggtTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACg                                                              <  1:1100448/151‑1 (MQ=255)                                                                                    gtggtTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGa                                                             <  1:1136737/151‑1 (MQ=255)                                                                                     tggtTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGCTGGAAGACGCGGGGGTACTTCCAATCGAAGTCGCCGTCTCTAACCTGAACATGGGCGTCGTGGTTGACGTTTCCCCGCACAAAGGAGAAGTGCGGAACCa                                                                >  1:182224/1‑147 (MQ=255)                                                                                      ggtTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGaa                                                            <  1:545842/150‑1 (MQ=255)                                                                                         ttGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAAcc                                                         <  1:466383/150‑1 (MQ=255)                                                                                         ttGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAAcc                                                         >  2:792862/1‑150 (MQ=255)                                                                                          tgtgCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCg                                                        <  2:1169024/150‑1 (MQ=255)                                                                                               cTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTCACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGaa                                                   <  2:1059919/150‑1 (MQ=255)                                                                                                 cggcggTAAAATTGCCCCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGGGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAAct                                                 <  2:343279/150‑1 (MQ=255)                                                                                                   gcggTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAActgct                                              <  2:978496/151‑1 (MQ=255)                                                                                                    cggTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAActgctg                                             >  2:360181/1‑151 (MQ=255)                                                                                                          aaTTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGAcc                                       >  2:292488/1‑151 (MQ=255)                                                                                                           aTTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCTATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCt                                      <  1:557412/151‑1 (MQ=255)                                                                                                           aTTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGGGGCGTGCGTAACCACCAAACCGGCGAACTGCTTGAGAcc                                       >  2:734593/1‑150 (MQ=255)                                                                                                            ttGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCtt                                     >  1:1069265/1‑151 (MQ=255)                                                                                                             tGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCtt                                     <  1:917286/150‑1 (MQ=255)                                                                                                               cACCCATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGTCGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTc                                    <  2:436701/149‑1 (MQ=255)                                                                                                                  ccATCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACt                               >  2:1131367/1‑151 (MQ=255)                                                                                                                    aTCTTCTTTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTg                              >  1:649832/1‑150 (MQ=255)                                                                                                                        tcttTAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGaaaa                          <  2:1219252/150‑1 (MQ=255)                                                                                                                          tttAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCg                       >  1:203227/1‑151 (MQ=255)                                                                                                                          tttAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCg                       >  2:271642/1‑151 (MQ=255)                                                                                                                           ttAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCg                       >  1:1188999/1‑150 (MQ=255)                                                                                                                            tAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGAc                     >  1:1048920/1‑151 (MQ=255)                                                                                                                            tAACACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGGGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGa                      >  2:655929/1‑150 (MQ=255)                                                                                                                             aaCACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGAc                     <  1:515890/150‑1 (MQ=255)                                                                                                                             aaCACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACg                    >  2:562077/1‑151 (MQ=255)                                                                                                                             aaCACGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACg                    >  2:67334/1‑151 (MQ=255)                                                                                                                              acacGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGt                   >  1:446628/1‑151 (MQ=255)                                                                                                                                acGATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTg                  >  2:975177/1‑150 (MQ=255)                                                                                                                                  gATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCtg               <  2:833870/151‑1 (MQ=255)                                                                                                                                  gATGGAAGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCAAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCtt               <  1:759151/151‑2 (MQ=255)                                                                                                                                       aaGACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCtgattg           >  1:385852/1‑150 (MQ=255)                                                                                                                                         gACGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATtgatg        <  2:572624/151‑1 (MQ=255)                                                                                                                                          aCGCGGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATtgatga       <  2:176981/151‑1 (MQ=255)                                                                                                                                           cgcgGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATtgatga       >  2:728428/1‑150 (MQ=255)                                                                                                                                           cgcgGGTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGaa      <  2:200968/151‑1 (MQ=255)                                                                                                                                              gggTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAgtg   <  1:9807/151‑1 (MQ=255)                                                                                                                                               ggTGTACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAgtgc  <  1:1088329/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGC  >  NC_000913/132340‑132632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑