Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,270,861	A→G	V330A (GTG→GCG)	chaA ←	calcium/sodium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,270,861	0	A	G	100.0%	207.6 / NA	55	V330A (GTG→GCG)	chaA	calcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/26);  total (29/26)

CCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTG  >  NC_000913/1270716‑1271003                                                                                                                                                  |                                                                                                                                               ccccGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATc                                                                                                                                           <  2:77343/151‑1 (MQ=255)   ccGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATcac                                                                                                                                         >  2:653676/1‑151 (MQ=255)   ccGATGACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACTTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATcac                                                                                                                                         <  2:1009245/151‑1 (MQ=255)     gATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATcacc                                                                                                                                        >  2:27173/1‑150 (MQ=255)     gATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATcacc                                                                                                                                        >  1:638208/1‑150 (MQ=255)     gATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAAAGGCCGCCATcacc                                                                                                                                        >  1:628968/1‑150 (MQ=255)      aTTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATcaccac                                                                                                                                      >  1:1065648/1‑151 (MQ=255)              cATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTg                                                                                                                              >  1:403016/1‑151 (MQ=255)               aTCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTg                                                                                                                              >  2:387843/1‑150 (MQ=255)                tCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGgc                                                                                                                            >  2:227858/1‑151 (MQ=255)                tCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGgc                                                                                                                            >  1:291896/1‑151 (MQ=255)                 cGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGgcg                                                                                                                           >  2:534190/1‑151 (MQ=255)                  ggaggaGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGgcgc                                                                                                                          <  1:95933/151‑1 (MQ=255)                  ggaggaGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGgcgc                                                                                                                          >  1:699631/1‑151 (MQ=255)                              aCTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCaa                                                                                                               >  1:1167664/1‑150 (MQ=255)                              aCTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCaa                                                                                                               >  1:729342/1‑150 (MQ=255)                                 gTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAAc                                                                                                             <  2:403004/149‑1 (MQ=255)                                      gCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAAtt                                                                                                      <  1:368308/151‑1 (MQ=255)                                       cAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAAtt                                                                                                      >  2:43596/1‑150 (MQ=255)                                       cAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTc                                                                                                     <  2:54821/151‑1 (MQ=255)                                       cAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTc                                                                                                     <  1:251918/151‑1 (MQ=255)                                        aaaTATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCg                                                                                                    >  1:390581/1‑151 (MQ=255)                                        aaaTATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCg                                                                                                    <  1:784403/151‑1 (MQ=255)                                            atCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTAc                                                                                                >  1:901758/1‑151 (MQ=255)                                               gTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCg                                                                                             >  1:126953/1‑151 (MQ=255)                                               gTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCg                                                                                             <  2:495233/151‑1 (MQ=255)                                                tcatcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGt                                                                                            >  2:1046350/1‑151 (MQ=255)                                                 catcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGt                                                                                            <  1:1209622/150‑1 (MQ=255)                                                     aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATa                                                                                        >  1:1096771/1‑150 (MQ=255)                                                       aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAg                                                                                     <  2:364246/151‑1 (MQ=255)                                                        tAGGCGGCAAACAGTGCCAGATGCGCTGCGGCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCATCACCATTTCTGGCGAACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAgg                                                                                    >  2:645799/1‑151 (MQ=255)                                                                  aCAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGt                                                                          >  2:288226/1‑151 (MQ=255)                                                                              gcgctgcgcCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTa                                                               <  1:462504/150‑1 (MQ=255)                                                                               cgctgcgcCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACg                                                             <  1:653692/151‑1 (MQ=255)                                                                                gctgcgcCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACg                                                             <  2:636511/150‑1 (MQ=255)                                                                                   gcgcCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTaa                                                         <  2:126954/151‑1 (MQ=255)                                                                                    cgcCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAg                                                        <  1:225760/151‑1 (MQ=255)                                                                                        aTTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGaa                                                    <  1:862308/151‑1 (MQ=255)                                                                                                      tACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAacac                                      >  2:1089215/1‑151 (MQ=255)                                                                                                      tACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAacac                                      >  2:1076301/1‑151 (MQ=255)                                                                                                       aCGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACt                                     >  2:418292/1‑151 (MQ=255)                                                                                                              gTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCa                               <  1:462803/150‑1 (MQ=255)                                                                                                                ggAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCaaa                             <  1:897682/150‑1 (MQ=255)                                                                                                                   gaAGGAGATTTGGCACAGCACTAAAGAGGCCGCCACCACCACCGTTTCTGGCGCGCCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATGAGCCTGACGACAGGTACGGTAATCGAAATGGTTGCTAACACGGAACCaaa                             <  2:683161/147‑1 (MQ=255)                                                                                                                     aGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAAc                         >  1:440568/1‑149 (MQ=255)                                                                                                                      ggAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAAc                         >  1:1053172/1‑148 (MQ=255)                                                                                                                      ggAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGa                      <  2:631163/151‑1 (MQ=255)                                                                                                                        agaTATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGAtt                    <  1:528783/151‑1 (MQ=255)                                                                                                                         gaTATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTc                   <  1:269119/151‑1 (MQ=255)                                                                                                                            atGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATc                >  1:143682/1‑151 (MQ=255)                                                                                                                                   aGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCt         >  2:75234/1‑151 (MQ=255)                                                                                                                                       cTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGaa      <  1:534212/150‑1 (MQ=255)                                                                                                                                         aaaGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAAc     <  2:6842/149‑1 (MQ=255)                                                                                                                                         aaaGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCt   <  2:699623/151‑1 (MQ=255)                                                                                                                                          aaGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTATCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTg  >  1:241843/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                               CCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTG  >  NC_000913/1270716‑1271003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑