Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 134,458 T→C V24A (GTC→GCC)  yacL → UPF0231 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913134,4580TC100.0% 267.4 / NA 75V24A (GTC→GCC) yacLUPF0231 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/35);  total (40/35)

TCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGAT  >  NC_000913/134313‑134605
                                                                                                                                                 |                                                                                                                                                   
tcCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGc                                                                                                                                                >  1:809240/1‑151 (MQ=255)
 ccGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCggg                                                                                                                                                 <  1:527809/149‑1 (MQ=255)
 ccGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCgg                                                                                                                                                  <  1:561834/148‑1 (MQ=255)
      aaGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGt                                                                                                                                          <  2:1085949/151‑1 (MQ=255)
      aaGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGt                                                                                                                                          >  2:773294/1‑151 (MQ=255)
      aaGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCAATGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGt                                                                                                                                          <  1:199079/151‑1 (MQ=255)
        ggCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGttt                                                                                                                                        <  1:674306/151‑1 (MQ=255)
         gCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGTGATATTACCGGAGTGGTAAAGGTGCGAATGTCCATGGGGCATGAAGTGGCCTGGCACTg                                                                                                                                            <  2:658372/146‑1 (MQ=255)
           tGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAAt                                                                                                                                     >  1:166165/1‑151 (MQ=255)
           tGCGATAATTACATCAATGGCGCAATGCGATTTAGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAGAGGAGCGTATGTCCATGGGGCATGAAGAGGCCGGCCACTGGTTTaa                                                                                                                                      >  2:872362/1‑150 (MQ=255)
            gCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATg                                                                                                                                    <  1:759260/151‑1 (MQ=255)
             cGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATg                                                                                                                                    >  2:1015529/1‑150 (MQ=255)
             cGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATg                                                                                                                                    >  2:1175134/1‑150 (MQ=255)
             cGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGa                                                                                                                                   <  1:1209640/151‑1 (MQ=255)
                    tACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTg                                                                                                                            >  2:731407/1‑151 (MQ=255)
                     aCATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTg                                                                                                                            >  1:233262/1‑150 (MQ=255)
                       aTCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTgaaa                                                                                                                         <  1:187550/151‑1 (MQ=255)
                         cAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTgaaag                                                                                                                        <  2:945790/150‑1 (MQ=255)
                                 cAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTgg                                                                                                               <  1:1075280/151‑1 (MQ=255)
                                   aTGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGcc                                                                                                             >  2:889020/1‑151 (MQ=255)
                                     gCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCctt                                                                                                           >  1:192834/1‑151 (MQ=255)
                                     gCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCct                                                                                                            <  2:1124593/150‑1 (MQ=255)
                                      cGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCcttg                                                                                                          >  2:1203636/1‑151 (MQ=255)
                                        aTTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCcttgct                                                                                                        >  1:480187/1‑151 (MQ=255)
                                        aTTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCcttgct                                                                                                        >  1:688488/1‑151 (MQ=255)
                                           tCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCcttgcttg                                                                                                      <  2:54588/150‑1 (MQ=255)
                                            cGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTtgat                                                                                                    <  2:527233/151‑1 (MQ=255)
                                            cGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTtga                                                                                                     <  1:482184/150‑1 (MQ=255)
                                            cGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGCGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGCGAAAGAAAACCTGGCCTTGCTtgat                                                                                                    >  2:135660/1‑151 (MQ=255)
                                             ggTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTtgatg                                                                                                   >  2:605509/1‑151 (MQ=255)
                                                gCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGaa                                                                                                 <  1:1098000/150‑1 (MQ=255)
                                                 cATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAg                                                                                                >  2:596425/1‑150 (MQ=255)
                                                  aTTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTg                                                                                              >  1:652184/1‑151 (MQ=255)
                                                  aTTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTg                                                                                              <  1:885777/151‑1 (MQ=255)
                                                   ttGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTgg                                                                                             >  2:613914/1‑151 (MQ=255)
                                                       cGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAAc                                                                                          <  2:1240257/150‑1 (MQ=255)
                                                          gAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAagc                                                                                      >  1:1248543/1‑151 (MQ=255)
                                                          gAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAagc                                                                                      <  1:951122/151‑1 (MQ=255)
                                                              agagGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAgcg                                                                                  >  1:314862/1‑151 (MQ=255)
                                                                agGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGcgca                                                                                <  1:94242/151‑1 (MQ=255)
                                                                    acacacTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGcgcacgca                                                                            <  2:803093/151‑1 (MQ=255)
                                                                    acacacTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGcgcacgca                                                                            <  1:891349/151‑1 (MQ=255)
                                                                      acacTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACt                                                                          <  1:683253/151‑1 (MQ=255)
                                                                          tATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGaa                                                                       >  2:1142445/1‑150 (MQ=255)
                                                                           aTGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGaa                                                                       >  2:1197766/1‑149 (MQ=255)
                                                                              gATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGt                                                                   >  1:246382/1‑150 (MQ=255)
                                                                                    gAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAAc                                                            <  1:10596/151‑1 (MQ=255)
                                                                                     aaTTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACg                                                           >  2:348724/1‑151 (MQ=255)
                                                                                         tCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTcc                                                       >  2:300257/1‑151 (MQ=255)
                                                                                            gcgcGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTgg                                                    >  1:718717/1‑151 (MQ=255)
                                                                                                 atatTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACg                                               >  1:866125/1‑151 (MQ=255)
                                                                                                   atTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACggg                                             <  1:541273/151‑1 (MQ=255)
                                                                                                      aCCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcag                                          <  1:1194621/151‑1 (MQ=255)
                                                                                                       ccGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcagg                                         >  2:331783/1‑151 (MQ=255)
                                                                                                         ggAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcagggc                                       <  1:807039/151‑1 (MQ=255)
                                                                                                         ggAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGGAGCGAACGGTCCTGGCAACgggcagggc                                       >  2:207714/1‑151 (MQ=255)
                                                                                                           aGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCAt                                     >  1:273339/1‑151 (MQ=255)
                                                                                                           aGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCAt                                     <  1:104894/151‑1 (MQ=255)
                                                                                                            gTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATg                                    <  2:552109/151‑1 (MQ=255)
                                                                                                              ggTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGa                                   >  2:193698/1‑150 (MQ=255)
                                                                                                                tAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGa                                   >  2:684960/1‑148 (MQ=255)
                                                                                                                tAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAAt                                 >  1:589095/1‑150 (MQ=255)
                                                                                                                 aaaGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATa                                <  2:190521/150‑1 (MQ=255)
                                                                                                                 aaaGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATa                                <  1:349197/150‑1 (MQ=255)
                                                                                                                   aGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATacac                             >  2:1019266/1‑151 (MQ=255)
                                                                                                                   aGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATacac                             >  1:301978/1‑151 (MQ=255)
                                                                                                                       gCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCt                          <  2:166171/150‑1 (MQ=255)
                                                                                                                          tATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtgg                      >  2:810621/1‑151 (MQ=255)
                                                                                                                                       cATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGa           >  2:104432/1‑149 (MQ=255)
                                                                                                                                        aTGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAaga        <  1:352063/151‑1 (MQ=255)
                                                                                                                                        aTGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAaga        <  1:105337/151‑1 (MQ=255)
                                                                                                                                         tGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAag         >  1:477735/1‑149 (MQ=255)
                                                                                                                                         tGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAag         >  1:991915/1‑149 (MQ=255)
                                                                                                                                          gAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTTAAAGGTAGCGAACGGTCCTGGCAGCGGGCAGGGCATGAATACACCCTGTGGATGGGCGGTGAAGAgg      <  2:887667/151‑1 (MQ=255)
                                                                                                                                              tGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGAt  >  1:306669/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGAT  >  NC_000913/134313‑134605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: