Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,490,800	A→G	intergenic (‑87/‑102)	gapC ← / → cybB	pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)/cytochrome b561

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,490,800	0	A	G	100.0%	290.0 / NA	76	intergenic (‑87/‑102)	gapC/cybB	pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)/cytochrome b561
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/40);  total (36/40)

GTAATCGACCCAACACCAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCACAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCT  >  NC_000913/1490653‑1490945                                                                                                                                                    |                                                                                                                                                  gTAATCGACCCAACACCAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAg                                                                                                                                                <  1:186203/151‑1 (MQ=255)   aaTCGACCCAACACCAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGt                                                                                                                                               <  2:1168987/150‑1 (MQ=255)   aaTCGACCCAACACCAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTg                                                                                                                                              >  1:577530/1‑151 (MQ=255)    aTCGACCCATCACCAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGa                                                                                                                                             <  2:733773/151‑1 (MQ=255)       gACCCAACACCAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAaac                                                                                                                                          <  1:715580/151‑1 (MQ=255)          ccAACACCAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAaacgta                                                                                                                                       <  2:51335/151‑1 (MQ=255)                ccAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAacgtaacgta                                                                                                                                  <  2:1124036/150‑1 (MQ=255)                   gTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTg                                                                                                                               >  2:1140301/1‑150 (MQ=255)                     cGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGaaa                                                                                                                            <  1:1226467/151‑1 (MQ=255)                        ccGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACg                                                                                                                         <  2:270043/151‑1 (MQ=255)                               gACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAAc                                                                                                                  <  1:599545/151‑1 (MQ=255)                                 ccAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACaa                                                                                                                <  2:1030378/151‑1 (MQ=255)                                  cAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAat                                                                                                               >  2:594400/1‑151 (MQ=255)                                    aaaCCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAatat                                                                                                             >  2:276058/1‑151 (MQ=255)                                    aaaCCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAatat                                                                                                             <  1:564849/151‑1 (MQ=255)                                            aaTACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGaa                                                                                                     <  1:642070/151‑1 (MQ=255)                                            aaTACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGa                                                                                                      >  2:1245242/1‑150 (MQ=255)                                            aaTACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGa                                                                                                      <  1:822185/150‑1 (MQ=255)                                                     tttACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTg                                                                                               >  1:205834/1‑148 (MQ=255)                                                          tCATGGTTTTCTCCTGCCAGGAACGTTCGGATGAAAATAGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAATCCTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGGGaaaa                                                                                         <  2:137909/149‑1 (MQ=255)                                                           cATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTGCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGcc                                                                                      <  2:416348/151‑1 (MQ=255)                                                            aTGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGccc                                                                                     >  2:408422/1‑151 (MQ=255)                                                                 tttCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTa                                                                                 <  2:863156/150‑1 (MQ=255)                                                                 tttCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTAt                                                                                >  2:295510/1‑151 (MQ=255)                                                                   tctcCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATg                                                                               <  1:866637/150‑1 (MQ=255)                                                                     tcCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGtt                                                                             >  1:629031/1‑150 (MQ=255)                                                                      ccTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGt                                                                              <  2:72193/148‑1 (MQ=255)                                                                      ccTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTa                                                                            <  2:9261/150‑1 (MQ=255)                                                                         gTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACg                                                                         <  1:611833/150‑1 (MQ=255)                                                                         gTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACg                                                                         <  1:140205/150‑1 (MQ=255)                                                                           cAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGaa                                                                      <  1:1154862/151‑1 (MQ=255)                                                                           cAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGa                                                                       >  1:204376/1‑150 (MQ=255)                                                                           cAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGa                                                                       >  2:164040/1‑150 (MQ=255)                                                                            aGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGaa                                                                      >  1:199390/1‑150 (MQ=255)                                                                                 cGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTAt                                                                <  1:556351/151‑1 (MQ=255)                                                                                   ttCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGt                                                              >  2:1204918/1‑151 (MQ=255)                                                                                           aaaaTTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGc                                                        >  1:822769/1‑149 (MQ=255)                                                                                           aaaaTTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGc                                                        >  1:854248/1‑149 (MQ=255)                                                                                                 gATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAAt                                                 <  1:205673/150‑1 (MQ=255)                                                                                                 gATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATg                                                <  2:913516/151‑1 (MQ=255)                                                                                                  aTCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGt                                               <  1:1103053/151‑1 (MQ=255)                                                                                                  aTCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGt                                               <  2:473201/151‑1 (MQ=255)                                                                                                   tCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGtt                                              >  1:412637/1‑151 (MQ=255)                                                                                                   tCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGtt                                              >  1:1185392/1‑151 (MQ=255)                                                                                                   tCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGt                                               <  2:785122/150‑1 (MQ=255)                                                                                                    ccTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGtt                                              >  1:539624/1‑150 (MQ=255)                                                                                                     cTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGAAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTa                                             >  2:1038164/1‑150 (MQ=255)                                                                                                       ttCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATgg                                          >  2:1104336/1‑151 (MQ=255)                                                                                                       ttCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATg                                           <  1:1140277/150‑1 (MQ=255)                                                                                                          cAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGaaa                                       <  2:118357/151‑1 (MQ=255)                                                                                                          cAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGaa                                        >  1:770786/1‑150 (MQ=255)                                                                                                            aGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAaaat                                     <  1:99399/151‑1 (MQ=255)                                                                                                             gCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAaaat                                     >  1:144486/1‑150 (MQ=255)                                                                                                               ttAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAaaataa                                   >  2:328330/1‑150 (MQ=255)                                                                                                                tAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAaaataaat                                 >  1:25398/1‑151 (MQ=255)                                                                                                                tAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAaaataa                                   <  2:1181981/149‑1 (MQ=255)                                                                                                                 aGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAaaataaat                                 <  2:714980/150‑1 (MQ=255)                                                                                                                 aGACCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAaaataaat                                 >  1:1161691/1‑150 (MQ=255)                                                                                                                   aCCAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATAtt                              <  1:312125/151‑1 (MQ=255)                                                                                                                    ccAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTc                             >  2:143646/1‑151 (MQ=255)                                                                                                                     cAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATAtt                              >  1:61982/1‑149 (MQ=255)                                                                                                                     cAGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCa                            <  2:597101/151‑1 (MQ=255)                                                                                                                      aGGATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCaa                           <  1:985517/151‑1 (MQ=255)                                                                                                                        gATGGCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAgg                         <  2:50456/151‑1 (MQ=255)                                                                                                                           ggCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGtt                       <  1:520427/150‑1 (MQ=255)                                                                                                                           ggCGGGATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTa                      <  2:1045508/151‑1 (MQ=255)                                                                                                                                gATGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAAt                 >  2:1142980/1‑151 (MQ=255)                                                                                                                                 aTGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATc                >  2:460542/1‑151 (MQ=255)                                                                                                                                  tGGGCAATCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATc                >  2:456100/1‑150 (MQ=255)                                                                                                                                       aaTCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCAtt          >  1:863901/1‑151 (MQ=255)                                                                                                                                       aaTCTCCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCAtt          >  1:641625/1‑151 (MQ=255)                                                                                                                                          ctcCATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCAc       >  2:1224088/1‑151 (MQ=255)                                                                                                                                            ccATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCActg     <  2:28435/151‑1 (MQ=255)                                                                                                                                            ccATTCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCActg     >  2:987158/1‑151 (MQ=255)                                                                                                                                               ttCTCGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCActggct  >  2:162694/1‑151 (MQ=255)                                                                                                                                                tctcGCAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCActgtct  >  2:45906/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GTAATCGACCCAACACCAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCACAGTGAAACGTAACGTACTGAAAACGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCT  >  NC_000913/1490653‑1490945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑