Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,494,068	A→G	E533E (GAA→GAG)	trg →	methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,494,068	0	A	G	100.0%	235.7 / NA	70	E533E (GAA→GAG)	trg	methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/38);  total (32/38)

GATTTCTGAAATGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCT  >  NC_000913/1493921‑1494214                                                                                                                                                    |                                                                                                                                                   gATTTCTGAAATGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGcc                                                                                                                                                  >  2:1226447/1‑150 (MQ=255) gATTTCTGAAATGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCt                                                                                                                                                 <  1:620999/151‑1 (MQ=255) gATTTCTGAAATGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGGATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGcc                                                                                                                                                  >  2:1165931/1‑150 (MQ=255)  aTTTCTGAAATGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTc                                                                                                                                                <  1:803765/151‑1 (MQ=255)    ttCTGAAATGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTcg                                                                                                                                               >  1:381838/1‑150 (MQ=255)     tCTGAAATGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGGTGTATTCCGGCTGCACAAACATTCTGTGTCGGCAGAGcc                                                                                                                                                  >  2:1051460/1‑146 (MQ=255)        gAAATGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAg                                                                                                                                          <  1:891414/151‑1 (MQ=255)           aTGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGACTCGCGGAGCgg                                                                                                                                       >  1:672947/1‑151 (MQ=255)            tGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGGTGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCggg                                                                                                                                      >  1:3431/1‑151 (MQ=255)            tGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCggg                                                                                                                                      >  1:11575/1‑151 (MQ=255)                  aGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGTCGGCACGATTAACTGAGGCGTGGGATGTTTTCCGTCTGCGCAAACAGTCTGTGTCTGCAGGGGCCCGCGGCGCGGGGGGAcc                                                                                                                                >  2:755291/1‑151 (MQ=255)                       aCGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCagtta                                                                                                                           <  1:87163/151‑1 (MQ=255)                        cGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGGGAACCagtt                                                                                                                            >  2:640102/1‑149 (MQ=255)                            aCAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTc                                                                                                                      <  1:391666/151‑1 (MQ=255)                              aGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGAGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGGACCAGTTAGttt                                                                                                                       >  2:1003489/1‑148 (MQ=255)                                aaTGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGCGGATGTATTCGGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGGTAGTTTCGCt                                                                                                                   >  2:551128/1‑150 (MQ=255)                                    cTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCCCCAACATTCTGTGTCGGCAGAGCCCCGCGGAGCGGGGGAACCAGTTTGTTTCGCTACgg                                                                                                               >  2:867912/1‑150 (MQ=255)                                     ttCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACgg                                                                                                               >  2:902908/1‑149 (MQ=255)                                      tctcTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGgt                                                                                                              <  2:719425/149‑1 (MQ=255)                                        tctGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTTGCTACGGtgtg                                                                                                           >  1:834517/1‑150 (MQ=255)                                         ctGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGgtgt                                                                                                            <  1:925824/148‑1 (MQ=255)                                           ggTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGaaa                                                                                                        <  2:9931/150‑1 (MQ=255)                                              agaaggGTCCTCAGCGGCGGAGGTGTCCCTTGAAGAACAGGCGGCTCGATTAACTGAGGCGGTGGATGTATTCCGTCGGCACAAACATCCTGTGTCAGCAGAGACTCGCGGAGCGTGTGATCCAGCTAGTTTCGCTAAGGTCTGaaaac                                                                                                      <  2:1110252/149‑2 (MQ=255)                                                aagaGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACTTTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAGCCAGTTAGTTTCGCAACGGTGTGAAAATGtt                                                                                                   <  2:784313/150‑1 (MQ=255)                                                    ggCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAag                                                                                               <  1:768355/150‑1 (MQ=255)                                                       cTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAaggagg                                                                                           >  1:1122/1‑151 (MQ=255)                                                        tCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAggg                                                                                          >  1:345526/1‑151 (MQ=255)                                                          aGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGAt                                                                                        <  2:170863/151‑1 (MQ=255)                                                           gcggcggcgGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATc                                                                                       <  2:381825/151‑1 (MQ=255)                                                           gcggcggcgGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATc                                                                                       <  1:1019472/151‑1 (MQ=255)                                                            cggcggcggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCg                                                                                      <  2:778666/151‑1 (MQ=255)                                                             ggctgcggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATc                                                                                       >  2:1076324/1‑149 (MQ=255)                                                               cggcggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACa                                                                                   <  1:1034525/151‑1 (MQ=255)                                                                 gcggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGa                                                                                 <  2:11573/151‑1 (MQ=255)                                                                        cccTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCtt                                                                           <  2:834511/150‑1 (MQ=255)                                                                        cccTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCtt                                                                           <  2:746982/150‑1 (MQ=255)                                                                        cccTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTc                                                                          <  1:938849/151‑1 (MQ=255)                                                                        cccTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTc                                                                          >  1:245059/1‑151 (MQ=255)                                                                                gaaCAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTc                                                                   >  2:484586/1‑150 (MQ=255)                                                                                 aaCAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGGCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTc                                                                   >  2:530864/1‑149 (MQ=255)                                                                                   cAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGaa                                                               <  1:1051454/151‑1 (MQ=255)                                                                                   cAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGaa                                                               <  2:816008/151‑1 (MQ=255)                                                                                   cAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGa                                                                >  2:106315/1‑150 (MQ=255)                                                                                    aGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGGTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGaa                                                               >  1:580280/1‑150 (MQ=255)                                                                                     ggcggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACg                                                             <  2:1110539/151‑1 (MQ=255)                                                                                      gcggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACgg                                                            <  2:650635/151‑1 (MQ=255)                                                                                       cggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGc                                                           <  1:1199671/151‑1 (MQ=255)                                                                                        ggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCa                                                          <  2:1169852/151‑1 (MQ=255)                                                                                           aCGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCAtt                                                        >  2:835542/1‑150 (MQ=255)                                                                                            cGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGa                                                      <  1:807225/151‑1 (MQ=255)                                                                                              aTTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGAtt                                                    <  1:416387/151‑1 (MQ=255)                                                                                                  aCTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCg                                                <  2:438179/151‑1 (MQ=255)                                                                                                    tGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGGGTGAAAATGTTCAAGGAGGGAACGAAAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGa                                               >  2:671431/1‑150 (MQ=255)                                                                                                     gAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGaa                                              >  1:1200222/1‑150 (MQ=255)                                                                                                        gCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAg                                           >  2:454861/1‑150 (MQ=255)                                                                                                             ggATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTaa                                     <  1:954840/151‑1 (MQ=255)                                                                                                                 gTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAatca                                  <  1:1165914/150‑1 (MQ=255)                                                                                                                       cGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTg                           <  1:907441/151‑1 (MQ=255)                                                                                                                       cGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTg                           >  2:801537/1‑151 (MQ=255)                                                                                                                       cGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTg                           >  2:223981/1‑151 (MQ=255)                                                                                                                       cGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTg                           >  2:1170168/1‑151 (MQ=255)                                                                                                                            gCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCaa                       <  2:588271/150‑1 (MQ=255)                                                                                                                                aaaCATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCaga                  <  2:520971/151‑1 (MQ=255)                                                                                                                                     ttCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCagaag                >  1:302098/1‑148 (MQ=255)                                                                                                                                         gtgtCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGaa            <  1:1073278/148‑1 (MQ=255)                                                                                                                                         gtgtCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAAc           <  1:1219421/149‑1 (MQ=255)                                                                                                                                          tgtCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTc        >  2:170945/1‑151 (MQ=255)                                                                                                                                           gtCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTc        <  2:1031115/150‑1 (MQ=255)                                                                                                                                             cGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGc      <  1:766546/150‑1 (MQ=255)                                                                                                                                                cAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCt  >  2:703235/1‑151 (MQ=255)                                                                                                                                                 agagCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCt  >  2:698076/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GATTTCTGAAATGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCT  >  NC_000913/1493921‑1494214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑