Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,719,070	A→G	I178T (ATT→ACT)	anmK ←	anhydro‑N‑acetylmuramic acid kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,719,070	0	A	G	100.0%	206.1 / NA	55	I178T (ATT→ACT)	anmK	anhydro‑N‑acetylmuramic acid kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/28);  total (27/28)

TTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACA  >  NC_000913/1718926‑1719216                                                                                                                                                 |                                                                                                                                                   tttcGCAGCAGTGGGAGAATACCTTTAACCGTACGTGCCCCCTCGGCATCTGTATCGTAAGGTTTACCGGCCTGACGCCAGGTCCAGGCCTCCAGCAGCATGTTACCAGGACCGGTACCGTAGCCCCCAACCGGCGGCCCAGGAGTGAGc                                                                                                                                               <  2:290592/150‑1 (MQ=255)       agcagTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGAc                                                                                                                                         >  1:713572/1‑150 (MQ=255)       agcagTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGAc                                                                                                                                         >  1:433222/1‑150 (MQ=255)          agTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGAc                                                                                                                                         >  2:672080/1‑147 (MQ=255)               gagaATAACTTTCCCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCg                                                                                                                                <  2:5637/151‑1 (MQ=255)               gagaATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCg                                                                                                                                <  1:1117392/151‑1 (MQ=255)                agaATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGc                                                                                                                                 >  2:631946/1‑149 (MQ=255)                          tACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAat                                                                                                                     >  1:907464/1‑151 (MQ=255)                             ccgcccgTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGGCGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATAtt                                                                                                                  <  1:790421/151‑1 (MQ=255)                               gcccgTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTg                                                                                                                 >  1:932909/1‑150 (MQ=255)                                cccgTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTAGCGATGCCGCCAATATTg                                                                                                                 >  2:130894/1‑149 (MQ=255)                                 ccgTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAgt                                                                                                              <  1:157299/151‑2 (MQ=255)                                      cccACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCAt                                                                                                         <  2:1181764/151‑1 (MQ=255)                                         aCTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATtcg                                                                                                      <  1:532084/151‑1 (MQ=255)                                            cGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATtcgt                                                                                                     <  2:203004/149‑1 (MQ=255)                                              gCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCt                                                                                                 >  1:173529/1‑151 (MQ=255)                                               cATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTc                                                                                                >  2:1140297/1‑151 (MQ=255)                                                         gTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTg                                                                                       >  2:90827/1‑150 (MQ=255)                                                         gTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGa                                                                                      <  1:809385/151‑1 (MQ=255)                                                             ggTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCca                                                                                  <  2:492444/151‑1 (MQ=255)                                                             ggTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGAAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCca                                                                                  <  2:1148508/151‑1 (MQ=255)                                                             ggTTTACCGGCCTGACGCCAGATCCACGCTGCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTTAGCAGTGACAGATGGGCGATGCTGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCca                                                                                  <  2:452620/151‑1 (MQ=255)                                                                 tACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCcagca                                                                               <  1:613939/150‑1 (MQ=255)                                                                   ccGGCCTGCCGCCAGATCCAGTCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCACAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATGTTGAGCACCATTCGTCGCTCGGTTGGGTGGGCCAGCAgcg                                                                            >  2:530383/1‑151 (MQ=255)                                                                   ccGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCcagcag                                                                              >  1:131476/1‑149 (MQ=255)                                                                     ggCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCa                                                                          <  2:344366/151‑1 (MQ=255)                                                                      gCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCat                                                                         <  1:1067995/151‑1 (MQ=255)                                                                        cTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCatg                                                                        >  2:590016/1‑150 (MQ=255)                                                                                aGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAAc                                                                 >  1:1187817/1‑149 (MQ=255)                                                                                 gATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGc                                                               >  1:839030/1‑150 (MQ=255)                                                                                   tCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAgg                                                            >  1:441912/1‑151 (MQ=255)                                                                                           tccatcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCg                                                    >  2:1203329/1‑151 (MQ=255)                                                                                             catcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGt                                                  <  1:711959/151‑1 (MQ=255)                                                                                              atcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTg                                                 <  2:346730/151‑1 (MQ=255)                                                                                                cAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTgc                                                <  1:944802/150‑1 (MQ=255)                                                                                                cAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTgc                                                <  1:110835/150‑1 (MQ=255)                                                                                                   cATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCt                                            >  1:1135828/1‑151 (MQ=255)                                                                                                   cATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCt                                            >  2:235266/1‑151 (MQ=255)                                                                                                    aTGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCtt                                           >  1:889726/1‑151 (MQ=255)                                                                                                         aCCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGtcc                                       <  1:510190/150‑1 (MQ=255)                                                                                                            aGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTccc                                   >  1:681157/1‑151 (MQ=255)                                                                                                                     aTCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAat                           >  2:846139/1‑150 (MQ=255)                                                                                                                          aGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATcgc                      <  1:727831/150‑1 (MQ=255)                                                                                                                           gCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATcgcg                     >  1:641499/1‑150 (MQ=255)                                                                                                                           gCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATcgcg                     >  1:256540/1‑150 (MQ=255)                                                                                                                             ccccAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACg                  <  1:872035/151‑1 (MQ=255)                                                                                                                              cccAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACgg                 <  1:1148038/151‑1 (MQ=255)                                                                                                                                cAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCg               >  2:388606/1‑151 (MQ=255)                                                                                                                                    cGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGaaaa           <  2:63355/151‑1 (MQ=255)                                                                                                                                     ggCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAAt          <  2:663405/151‑1 (MQ=255)                                                                                                                                     ggCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAAt          <  2:989872/151‑1 (MQ=255)                                                                                                                                      gCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATc         >  1:1032459/1‑151 (MQ=255)                                                                                                                                             aGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGAc   >  1:302389/1‑150 (MQ=255)                                                                                                                                             aGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACa  <  2:433210/151‑1 (MQ=255)                                                                                                                                             aGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACa  <  1:353970/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   TTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACA  >  NC_000913/1718926‑1719216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑