Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,081,148	T→C	S39G (AGC→GGC)	sbmC ←	DNA gyrase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,081,148	0	T	C	100.0%	198.3 / NA	52	S39G (AGC→GGC)	sbmC	DNA gyrase inhibitor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/34);  total (18/34)

CAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAG  >  NC_000913/2081005‑2081294                                                                                                                                                |                                                                                                                                                   cAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAATTACCCGTCACCGTCACGACTGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATATACAGCAACCCACTCCTTCGGCACATTATTTTTGCCATCTa                                                                                                                                               <  2:835139/149‑1 (MQ=255)  aGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTTCCATCTAccc                                                                                                                                            >  2:470189/1‑151 (MQ=255)   gAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCca                                                                                                                                           <  1:1040436/151‑1 (MQ=255)       gACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCAcat                                                                                                                                        <  2:48571/150‑1 (MQ=255)           cccTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACAtcatca                                                                                                                                   <  1:846020/151‑1 (MQ=255)               cACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTg                                                                                                                               <  2:1193475/151‑1 (MQ=255)               cACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTg                                                                                                                               <  2:1107244/151‑1 (MQ=255)               cACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTg                                                                                                                               <  2:218527/151‑1 (MQ=255)                  tattgtccggAAGCGTAAAGTAACCCGCCACCCTCACGACGTTGTCGCAGCGTAATTTCTCGCCGGGTGTTTCATCTGGATTGTCGTAATAGCCAGCAACCCACGCCTTCGGCTCAATATTTTTGCCATCTACCCCCATCATGAAATGc                                                                                                                              <  2:418331/141‑1 (MQ=255)                     tttCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGGGATTCATCGCGATTGTCGTAAAAGACAACAAACCACTCCTTTGGCATAATATTTTTGCCGTCTACCCACATCATCAACCTCTCaa                                                                                                                          >  2:79562/1‑150 (MQ=255)                             aGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCctttct                                                                                                                 <  1:871448/151‑1 (MQ=255)                                  aaaGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTAc                                                                                                             <  1:802728/150‑1 (MQ=255)                                        aCCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGt                                                                                                      <  1:982136/151‑1 (MQ=255)                                         cccGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGtt                                                                                                     <  1:339517/151‑1 (MQ=255)                                          ccGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAAACCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGtt                                                                                                     >  2:1193977/1‑150 (MQ=255)                                           cGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTcc                                                                                                   >  1:92727/1‑151 (MQ=255)                                                     acgacgGTGTCGCAGCGTAATTTTTCGGCGGGGGTTTCATCTGGGTTGTCGGAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAACCTTTCTTTACCGTCTGTTCCCACggg                                                                                             >  1:1107290/1‑147 (MQ=255)                                                       gacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACg                                                                                       <  1:303699/151‑1 (MQ=255)                                                       gacgGTGTCGCAGCGTAATTTTTCGGCGGGCGTTTCATTAGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTTCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGGCTGTTTCCACGGGCCaa                                                                                         >  2:534459/1‑149 (MQ=255)                                                         cgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACgag                                                                                     <  1:68077/151‑1 (MQ=255)                                                         cgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACgag                                                                                     <  2:754044/151‑1 (MQ=255)                                                           gtgtCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGAt                                                                                   <  2:1112366/151‑1 (MQ=255)                                                                       aaTTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCaa                                                                       <  1:694025/151‑1 (MQ=255)                                                                           tttCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACgg                                                                    <  2:928386/150‑1 (MQ=255)                                                                             tCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTa                                                                  <  2:1088155/150‑1 (MQ=255)                                                                             tCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTAc                                                                 >  1:1024221/1‑151 (MQ=255)                                                                                gcggGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGtt                                                              <  1:517155/151‑1 (MQ=255)                                                                                gcggGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGtt                                                              >  1:423799/1‑151 (MQ=255)                                                                                 cggGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGttt                                                             >  2:702338/1‑151 (MQ=255)                                                                                  gggTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGttt                                                             >  2:1137701/1‑150 (MQ=255)                                                                                               ggATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTaa                                               <  1:855427/151‑1 (MQ=255)                                                                                                gATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAAt                                              <  2:175742/151‑1 (MQ=255)                                                                                                     tCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGt                                         >  1:87260/1‑151 (MQ=255)                                                                                                      cGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGt                                         <  2:892757/150‑1 (MQ=255)                                                                                                      cGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGt                                         <  1:967761/150‑1 (MQ=255)                                                                                                            aGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCa                                   <  1:882031/150‑1 (MQ=255)                                                                                                               cAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGAc                               <  1:837112/151‑1 (MQ=255)                                                                                                                   aaCCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCt                           <  2:299784/151‑1 (MQ=255)                                                                                                                   aaCCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCt                           <  2:955417/151‑1 (MQ=255)                                                                                                                   aaCCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCt                           >  1:249087/1‑151 (MQ=255)                                                                                                                     cccACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTcc                         <  2:356996/151‑1 (MQ=255)                                                                                                                          tCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTg                     >  1:783456/1‑150 (MQ=255)                                                                                                                           ccTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTg                     <  1:1150189/149‑1 (MQ=255)                                                                                                                           ccTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGAt                   >  2:9968/1‑151 (MQ=255)                                                                                                                               cGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTg               <  1:252579/151‑1 (MQ=255)                                                                                                                                  cacaATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGttt            >  1:480920/1‑151 (MQ=255)                                                                                                                                    caATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGttttt          >  2:1127738/1‑151 (MQ=255)                                                                                                                                    caATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGtttt           >  1:953042/1‑150 (MQ=255)                                                                                                                                      atatTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTtat        <  1:442816/151‑1 (MQ=255)                                                                                                                                       tatTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTtat        >  1:132946/1‑150 (MQ=255)                                                                                                                                           tttGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATAc    <  1:800934/150‑1 (MQ=255)                                                                                                                                             tGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAg  <  2:809848/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                   CAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAG  >  NC_000913/2081005‑2081294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑