Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,130,464	T→C	T139A (ACC→GCC)	wcaD ←	putative colanic acid polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,130,464	0	T	C	100.0%	299.5 / NA	80	T139A (ACC→GCC)	wcaD	putative colanic acid polymerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/39);  total (41/39)

ATTAATGCCAGAGCGAAAAATGCCGGTTCGAAATAAAGTGCTGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATAT  >  NC_000913/2130325‑2130610                                                                                                                                            |                                                                                                                                                   aTTAATGCCAGAGCGAAAAATGCCGGTTCGAAATAAAGTGCTGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAAttt                                                                                                                                         <  1:113598/151‑1 (MQ=255)  ttAATGCCAGAGCGAAAAATGCCGGTTCGAAATAAAGTGCTGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTtg                                                                                                                                        >  1:633250/1‑151 (MQ=255)       gCCAGAGCGAAAAATGCCGGTTCGAAATAAAGTGCTGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCa                                                                                                                                   <  1:726626/151‑1 (MQ=255)        ccAGAGCGAAAAATGCCGGTTCGAAATAAAGTGCTGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCAt                                                                                                                                  <  1:1231960/151‑1 (MQ=255)          agagCGAAAAATGCCGGTTCGAAATAAAGTGCTGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCAtct                                                                                                                                <  1:287741/151‑1 (MQ=255)            agCGAAAAATGCCGGTTCGAAATAAAGTGCTGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTcc                                                                                                                              >  2:860892/1‑151 (MQ=255)                aaaaaTGCCGGTTCGAAATAAAGTGCTGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCAcc                                                                                                                           >  1:566960/1‑150 (MQ=255)                  aaaTGCCGGTTCGAAATAAAGTGCTGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCAccgcc                                                                                                                        >  1:1068093/1‑151 (MQ=255)                      gCCGGTTCGAAATAAAGTGCTGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCa                                                                                                                    <  1:476459/151‑1 (MQ=255)                       ccGGTTCGAAATAAAGTGCTGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCgccgcc                                                                                                                     >  2:511487/1‑149 (MQ=255)                        cGGTTCGAAATAAAGTGCTGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAgc                                                                                                                  >  1:445443/1‑151 (MQ=255)                        cGGTTCGAAATAAAGTGCTGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAgc                                                                                                                  >  1:162684/1‑151 (MQ=255)                             cGAAATAAAGTGCTGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGccac                                                                                                             >  1:253318/1‑151 (MQ=255)                                   aaaGTGCTGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGccaccacca                                                                                                        <  1:939902/150‑1 (MQ=255)                                       tGCTGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGccc                                                                                                    >  2:475829/1‑150 (MQ=255)                                        gCTGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCg                                                                                                   >  1:198679/1‑150 (MQ=255)                                         atGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGac                                                                                                 >  1:397344/2‑151 (MQ=255)                                           gTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGacca                                                                                               <  1:1143976/151‑1 (MQ=255)                                             cGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGaccacc                                                                                             >  1:959339/1‑151 (MQ=255)                                              gTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGaccacc                                                                                             >  1:605204/1‑150 (MQ=255)                                              gTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGAccacca                                                                                            <  1:1174113/151‑1 (MQ=255)                                                  gcTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGAt                                                                                         >  1:638721/1‑150 (MQ=255)                                                     tGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATaaa                                                                                      <  2:692300/150‑1 (MQ=255)                                                     tGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATaaa                                                                                      >  1:546073/1‑150 (MQ=255)                                                     tGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATaa                                                                                       <  2:137266/149‑1 (MQ=255)                                                       ccgccgAATTTAATGAAATTCAGCACATAGCTTTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCCCCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCTCCAGATAAAAGa                                                                                   <  2:287487/151‑1 (MQ=255)                                                        cgccgAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGaa                                                                                  <  2:633229/151‑1 (MQ=255)                                                         gccgAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGaa                                                                                  >  1:147930/1‑150 (MQ=255)                                                           cgAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACg                                                                               >  1:341063/1‑151 (MQ=255)                                                           cgAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACg                                                                               >  1:1198367/1‑151 (MQ=255)                                                            gAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGt                                                                              <  1:996967/151‑1 (MQ=255)                                                            gAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGt                                                                              <  2:895753/151‑1 (MQ=255)                                                            gAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGt                                                                              >  2:838758/1‑151 (MQ=255)                                                            gAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGt                                                                              >  2:433223/1‑151 (MQ=255)                                                                 tAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAatat                                                                         >  1:728378/1‑151 (MQ=255)                                                                  aaTGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAatat                                                                         <  2:292184/150‑1 (MQ=255)                                                                  aaTGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATc                                                                        >  1:155966/1‑151 (MQ=255)                                                                   aTGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCt                                                                       >  2:378469/1‑151 (MQ=255)                                                                     gAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTg                                                                     <  1:639496/151‑1 (MQ=255)                                                                       aaTTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCCCCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCg                                                                   <  1:974453/151‑1 (MQ=255)                                                                       aaTTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCg                                                                   <  1:302354/151‑1 (MQ=255)                                                                          tCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATg                                                                >  2:1124143/1‑151 (MQ=255)                                                                           cAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATgg                                                               >  2:949568/1‑151 (MQ=255)                                                                           cAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATgg                                                               >  1:758455/1‑151 (MQ=255)                                                                           cAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATg                                                                <  2:747335/150‑1 (MQ=255)                                                                               acaTAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTtgt                                                           >  2:855773/1‑151 (MQ=255)                                                                                 aTAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTtgtgt                                                         <  1:99238/151‑1 (MQ=255)                                                                                        ttGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGaa                                                   <  1:381745/150‑1 (MQ=255)                                                                                         tGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGaaa                                                  <  1:431195/150‑1 (MQ=255)                                                                                             gTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTg                                               <  2:605181/149‑1 (MQ=255)                                                                                              tAAAACAGATATTTCGAAATCGACTCCATAAAACTTCCGCCAACGGCGAGGATAATTTGTGCCATCCCCACCGCCGCCAGCGCCACCCCCAGCCCGACCACCAGATAAAAGAAACGTAATATCGTGCTAAGGTTGTGTGGCGAAATTGtt                                             <  2:864419/150‑1 (MQ=255)                                                                                               aaaTCAGATATTTCGAAATTGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGtttt                                           >  2:845813/1‑151 (MQ=255)                                                                                               aaaTCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGtttt                                           <  1:853100/151‑1 (MQ=255)                                                                                                 aTCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTa                                          >  1:477472/1‑150 (MQ=255)                                                                                                  tCAGATATTTCGAAATCGGCTCCATAATACTGCGGGCTCCGGCGAGGATAATTTGTGCCATCTCCTCCGCCGCCAGCGCCACCGCCAGCGCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTAGCTAAATTGTTATaa                                         <  2:274177/150‑1 (MQ=255)                                                                                                   cAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTaaaa                                       <  1:385263/151‑1 (MQ=255)                                                                                                    aGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTaaaa                                       >  2:704615/1‑150 (MQ=255)                                                                                                     gATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACg                                     <  2:1222479/151‑1 (MQ=255)                                                                                                     gATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACg                                     <  2:583602/151‑1 (MQ=255)                                                                                                     gATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACg                                     <  1:898160/151‑1 (MQ=255)                                                                                                        atTTCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGaa                                   <  1:949571/150‑1 (MQ=255)                                                                                                         tttCGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGAAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAAt                                  >  1:323891/1‑150 (MQ=255)                                                                                                            cGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACt                               <  2:1196722/150‑1 (MQ=255)                                                                                                            cGAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTc                              >  1:1188756/1‑151 (MQ=255)                                                                                                             gAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTcc                             >  2:5415/1‑151 (MQ=255)                                                                                                             gAAATCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTcc                             >  1:827835/1‑151 (MQ=255)                                                                                                               aaTCGACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCcac                           <  2:138293/151‑1 (MQ=255)                                                                                                                   gACTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACAcc                        >  1:788920/1‑150 (MQ=255)                                                                                                                    tcTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCCCCACCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGGTGGTTGTGTGACGAAATTGATTTAAAACGAATACTCcacaac                        >  2:187323/2‑147 (MQ=255)                                                                                                                    aCTCCATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCAt                      <  1:1232980/151‑1 (MQ=255)                                                                                                                       ccATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGcc                   >  1:1062781/1‑151 (MQ=255)                                                                                                                        cATAATACTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGcc                   >  1:451754/1‑150 (MQ=255)                                                                                                                         ataataCTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCaa                 <  1:475846/151‑1 (MQ=255)                                                                                                                           aataCTGCTGCCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCaat                >  2:216510/1‑150 (MQ=255)                                                                                                                                 gctgcCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATc         <  2:253326/151‑1 (MQ=255)                                                                                                                                  ctgcCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATc         >  2:596743/1‑150 (MQ=255)                                                                                                                                    gcCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATcaca      <  1:289690/151‑1 (MQ=255)                                                                                                                                    gcCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATca        <  2:626052/149‑1 (MQ=255)                                                                                                                                    gcCACCGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATca        <  1:855778/149‑1 (MQ=255)                                                                                                                                        ccGGCGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAatat  >  1:142592/1‑151 (MQ=255)                                                                                                                                            |                                                                                                                                                   ATTAATGCCAGAGCGAAAAATGCCGGTTCGAAATAAAGTGCTGTCGTGCGCTTGCCGCCGAATTTAATGAAATTCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATAT  >  NC_000913/2130325‑2130610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑