Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,223,301	T→C	F373L (TTC→CTC)	dld →	D‑lactate dehydrogenase, FAD‑binding, NADH independent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,223,301	0	T	C	100.0%	271.1 / NA	74	F373L (TTC→CTC)	dld	D‑lactate dehydrogenase, FAD‑binding, NADH independent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/38);  total (36/38)

CTTCCTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGTTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTT  >  NC_000913/2223159‑2223444                                                                                                                                               |                                                                                                                                                cTTCCTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGc                                                                                                                                         >  1:422451/1‑151 (MQ=255)    ccTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCa                                                                                                                                       <  2:192345/150‑1 (MQ=255)    ccTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCAt                                                                                                                                      >  1:1115717/1‑151 (MQ=255)    ccTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCAt                                                                                                                                      >  2:179892/1‑151 (MQ=255)       gatgatTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTa                                                                                                                                   <  2:169449/151‑1 (MQ=255)        atgatTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTAc                                                                                                                                  <  1:503355/151‑1 (MQ=255)         tgattgatAAGTTAGGCACCGTCAAGACGCCGTTCTTCTTTAATCTCCAGGGACGCACCGATGAGATGTTGGTGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGCCACCTGCTCCCAAGCCATTTa                                                                                                                                   >  2:71756/1‑149 (MQ=255)         tgattgatAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTAcc                                                                                                                                 >  2:894385/1‑151 (MQ=255)                   gTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAACTTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATg                                                                                                                       >  1:243605/1‑151 (MQ=255)                   gTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATg                                                                                                                       <  1:221187/151‑1 (MQ=255)                    ttAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATg                                                                                                                       >  1:43423/1‑150 (MQ=255)                      aGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGaaa                                                                                                                    <  2:1193352/151‑1 (MQ=255)                      aGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGaaa                                                                                                                    <  1:840898/151‑1 (MQ=255)                      aGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGaa                                                                                                                     >  1:582910/1‑150 (MQ=255)                       ggCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGaaaa                                                                                                                   <  1:1106864/151‑1 (MQ=255)                                aaGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGcgcg                                                                                                          <  1:257103/151‑1 (MQ=255)                                aaGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGcgcg                                                                                                          <  2:158688/151‑1 (MQ=255)                                   aTGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATa                                                                                                       <  1:1194514/151‑1 (MQ=255)                                   aTGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATa                                                                                                       <  1:676163/151‑1 (MQ=255)                                     gCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATaaa                                                                                                     <  2:931504/151‑1 (MQ=255)                                     gCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATaa                                                                                                      <  2:1152536/150‑1 (MQ=255)                                        gTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATAc                                                                                                  >  1:1073654/1‑151 (MQ=255)                                        gTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACAGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATAAAAAACTGGCGCGATAAAt                                                                                                    >  2:593614/1‑149 (MQ=255)                                          tcttctTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATAct                                                                                                 <  1:343738/150‑2 (MQ=255)                                          tcttctTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACg                                                                                                 >  2:1189327/1‑150 (MQ=255)                                          tcttctTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACg                                                                                                 >  1:596115/1‑150 (MQ=255)                                            ttcttTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGc                                                                                              >  2:652775/1‑151 (MQ=255)                                               tttAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGcatc                                                                                           >  2:794724/1‑151 (MQ=255)                                                 tAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGcatcat                                                                                         >  1:427605/1‑151 (MQ=255)                                                 tAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGcatca                                                                                          <  2:8294/150‑1 (MQ=255)                                                       cAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATctgctg                                                                                   <  1:12359/151‑1 (MQ=255)                                                       cAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATct                                                                                       <  2:696099/147‑1 (MQ=255)                                                        aaGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATctgct                                                                                    <  2:687902/149‑1 (MQ=255)                                                        aaGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGt                                                                                  >  2:567500/1‑151 (MQ=255)                                                         aGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGtt                                                                                 >  1:279911/1‑151 (MQ=255)                                                            gACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTaa                                                                               <  1:981698/150‑1 (MQ=255)                                                             aCGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTaaa                                                                              >  1:79349/1‑150 (MQ=255)                                                                  ccGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATggc                                                                        <  2:1057984/151‑1 (MQ=255)                                                                    gatgcgatgcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATggcgg                                                                      <  2:1178430/151‑1 (MQ=255)                                                                     atgcgatgcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATggcggg                                                                     >  1:1066578/1‑151 (MQ=255)                                                                     atgcgatgcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATggcggg                                                                     <  1:206242/151‑1 (MQ=255)                                                                          atgcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATg                                                                <  2:520055/151‑1 (MQ=255)                                                                            gcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATgg                                                               >  1:141535/1‑150 (MQ=255)                                                                               ggAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCg                                                             <  2:994164/149‑1 (MQ=255)                                                                                gagaAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTgg                                                          <  2:422438/151‑1 (MQ=255)                                                                                 agaAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTggg                                                         >  1:519653/1‑151 (MQ=255)                                                                                         aaaTTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGcc                                                  <  2:1199373/150‑1 (MQ=255)                                                                                         aaaTTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGcc                                                  >  1:809816/1‑150 (MQ=255)                                                                                             tcttcCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCACGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAAt                                              <  2:923851/150‑1 (MQ=255)                                                                                                tccgtccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGGTGGCGGGGGTGCAGCCaaa                                               >  2:634226/1‑146 (MQ=255)                                                                                                tccgtccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATc                                             <  2:805493/148‑1 (MQ=255)                                                                                                tccgtccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTg                                          >  1:676298/1‑151 (MQ=255)                                                                                                   gtccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCt                                       >  1:1135414/1‑151 (MQ=255)                                                                                                    tccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtg                                      >  1:280823/1‑151 (MQ=255)                                                                                                    tccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCt                                       <  1:429856/150‑1 (MQ=255)                                                                                                      cgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtgg                                     >  2:796526/1‑150 (MQ=255)                                                                                                       gCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtggtg                                   <  2:741482/151‑1 (MQ=255)                                                                                                        cATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtggtg                                   <  2:868716/150‑1 (MQ=255)                                                                                                         aTTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGGCTGGGCGAAGCCaaaatgggggcggggg                                  >  2:694771/1‑137 (MQ=255)                                                                                                                 gACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTAttt                           <  1:894387/149‑1 (MQ=255)                                                                                                                   ccGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCaa                        >  1:509263/1‑150 (MQ=255)                                                                                                                   ccGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGATTATTTTaa                        >  2:117085/1‑150 (MQ=255)                                                                                                                    cGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGAGAAAGCGGGGGGGAAGCCAAATCGTGGCTGGTGGGGTAtttttaa                       >  2:46077/1‑146 (MQ=255)                                                                                                                       gCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAg                    <  2:1237267/150‑1 (MQ=255)                                                                                                                       gCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGGGGGCGAAGCCAAATCGTGGCTGGGGGGTTTTTTCAAACAgg                   >  2:1217384/1‑151 (MQ=255)                                                                                                                               aaaaTTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGc           <  1:221120/151‑1 (MQ=255)                                                                                                                                  aTTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGa         >  2:443640/1‑150 (MQ=255)                                                                                                                                    tCGGTCCCCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGAtt       <  1:634247/150‑1 (MQ=255)                                                                                                                                    tCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGAtt       <  2:411969/150‑1 (MQ=255)                                                                                                                                     cGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGAttt      >  2:67053/1‑150 (MQ=255)                                                                                                                                     cGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGAttt      >  1:906125/1‑150 (MQ=255)                                                                                                                                       gTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCtt   <  1:659752/151‑1 (MQ=255)                                                                                                                                        tCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGCAGGGGctttcttt  >  2:967692/1‑151 (MQ=255)                                                                                                                                         cACCTGCTCCCCATCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCttt  <  1:312451/150‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                CTTCCTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGTTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTT  >  NC_000913/2223159‑2223444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑