Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,858,929	A→C	T613P (ACC→CCC)	mutS →	methyl‑directed mismatch repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,858,929	0	A	C	100.0%	309.4 / NA	81	T613P (ACC→CCC)	mutS	methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (46/35);  total (46/35)

ACCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCACCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGC  >  NC_000913/2858782‑2859071                                                                                                                                                    |                                                                                                                                               aCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCg                                                                                                                                             <  1:327278/151‑1 (MQ=255)   ccTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGt                                                                                                                                           >  1:963713/1‑151 (MQ=255)    cTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTc                                                                                                                                          >  2:157197/1‑151 (MQ=255)     tGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTcc                                                                                                                                         >  2:562045/1‑151 (MQ=255)      gAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCg                                                                                                                                        <  1:739797/151‑1 (MQ=255)          tACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAAc                                                                                                                                     >  1:672301/1‑150 (MQ=255)            cacCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATg                                                                                                                                  <  2:767133/151‑1 (MQ=255)              ccTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTCCCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATg                                                                                                                                  <  2:147935/149‑1 (MQ=255)              ccTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATgg                                                                                                                                 <  2:640472/150‑1 (MQ=255)                 gCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGc                                                                                                                               <  1:1000628/149‑1 (MQ=255)                         ttCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAg                                                                                                                       >  2:1192633/1‑149 (MQ=255)                         ttCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGt                                                                                                                      >  1:898951/1‑150 (MQ=255)                          tCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGt                                                                                                                      <  2:268137/149‑1 (MQ=255)                          tCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGt                                                                                                                      <  2:332010/149‑1 (MQ=255)                          tCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGt                                                                                                                      <  1:799891/149‑1 (MQ=255)                             ttGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCt                                                                                                                  >  1:9516/1‑150 (MQ=255)                              tGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCta                                                                                                                 <  1:913155/150‑1 (MQ=255)                                aTAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTatat                                                                                                              >  2:325229/1‑151 (MQ=255)                                aTAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTatat                                                                                                              >  2:40513/1‑151 (MQ=255)                                 tAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATg                                                                                                             >  2:91675/1‑151 (MQ=255)                                   aaCCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATgcg                                                                                                           >  1:449643/1‑151 (MQ=255)                                        ggCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGcc                                                                                                         >  1:917606/1‑148 (MQ=255)                                        agCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGcc                                                                                                         >  1:1189963/2‑148 (MQ=255)                                         gCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGAc                                                                                                     >  1:666878/1‑151 (MQ=255)                                          cATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGAcc                                                                                                    >  2:851915/1‑151 (MQ=255)                                            ttCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGc                                                                                                  >  1:574851/1‑151 (MQ=255)                                                cATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTg                                                                                              <  2:717015/151‑1 (MQ=255)                                                cATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTg                                                                                              >  1:619217/1‑151 (MQ=255)                                                cATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTg                                                                                              >  2:622815/1‑151 (MQ=255)                                                cATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTg                                                                                              <  1:483299/151‑1 (MQ=255)                                                     ccGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTgc                                                                                         >  1:563249/1‑151 (MQ=255)                                                      cGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTgcg                                                                                        >  2:819274/1‑151 (MQ=255)                                                         aGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTg                                                                                     <  2:840193/151‑1 (MQ=255)                                                               ccATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGcc                                                                               >  1:1100668/1‑151 (MQ=255)                                                               ccATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGACAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACACGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGcc                                                                               >  2:133084/1‑151 (MQ=255)                                                                cATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCt                                                                              <  1:243854/151‑1 (MQ=255)                                                                   ccGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTAc                                                                            <  1:224098/150‑1 (MQ=255)                                                                     ggTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATc                                                                         <  2:1106952/151‑1 (MQ=255)                                                                         gTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCa                                                                     <  1:856334/151‑1 (MQ=255)                                                                            gAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGc                                                                   <  1:387988/150‑1 (MQ=255)                                                                            gAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGc                                                                   <  1:929877/150‑1 (MQ=255)                                                                            gAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCt                                                                  <  1:991671/151‑1 (MQ=255)                                                                             aaCAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGCAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATCGCCTACATCGGCAGCt                                                                  >  2:1113249/1‑150 (MQ=255)                                                                               cAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATg                                                               >  2:831680/1‑151 (MQ=255)                                                                                 aGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGt                                                              >  2:430098/1‑150 (MQ=255)                                                                                 aGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTa                                                             <  2:759469/151‑1 (MQ=255)                                                                                   tACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTAcc                                                           >  1:983475/1‑151 (MQ=255)                                                                                       gAATGAGCCATTTATCTCCACCCCGCTGAGTCTGTCTCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTTTCGGca                                                       <  2:856100/151‑1 (MQ=255)                                                                                        aaTGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGcac                                                      >  2:585360/1‑151 (MQ=255)                                                                                           gAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACaa                                                    <  1:707846/150‑1 (MQ=255)                                                                                           gAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACaa                                                    <  1:177738/150‑1 (MQ=255)                                                                                            aGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACaaaa                                                  <  1:1111531/151‑1 (MQ=255)                                                                                            aGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACaaa                                                   >  2:434422/1‑150 (MQ=255)                                                                                            aGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACaaa                                                   >  1:83948/1‑150 (MQ=255)                                                                                                aTTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTc                                              <  2:504377/151‑1 (MQ=255)                                                                                                aTTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTc                                              <  1:413460/151‑1 (MQ=255)                                                                                                 tttATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCg                                             >  2:453769/1‑151 (MQ=255)                                                                                                    aTCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCgag                                           <  2:398493/150‑1 (MQ=255)                                                                                                    aTCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCg                                             >  2:1136702/1‑148 (MQ=255)                                                                                                          aaCCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGAc                                    >  1:7478/1‑151 (MQ=255)                                                                                                           aCCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGAcc                                   >  1:1098640/1‑151 (MQ=255)                                                                                                           aCCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGAcc                                   >  1:826612/1‑151 (MQ=255)                                                                                                             ccGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCt                                  >  2:720683/1‑150 (MQ=255)                                                                                                             ccGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTa                                 <  1:856084/151‑1 (MQ=255)                                                                                                                 tGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTatcg                              >  1:388724/1‑150 (MQ=255)                                                                                                                 tGAATCTGTCGCAGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTAAATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTa                                 >  2:738011/1‑147 (MQ=255)                                                                                                                  gAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTatcga                             <  2:524489/150‑1 (MQ=255)                                                                                                                     tCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGc                         >  1:755797/1‑151 (MQ=255)                                                                                                                        gTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGc                         <  2:427132/148‑1 (MQ=255)                                                                                                                          cgccgcAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCtt                    <  1:418292/151‑1 (MQ=255)                                                                                                                             cgcAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTAc                 >  1:369574/1‑151 (MQ=255)                                                                                                                                   gccgcATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGt           >  2:912078/1‑151 (MQ=255)                                                                                                                                   gccgcATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGt           >  1:150482/1‑151 (MQ=255)                                                                                                                                    ccgcATGTTGATCATCCCCGGTCCGAATATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGt           >  1:68367/1‑150 (MQ=255)                                                                                                                                    ccgcATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTa          <  1:195218/151‑1 (MQ=255)                                                                                                                                     cgcATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCcgcg            >  2:253947/1‑148 (MQ=255)                                                                                                                                     cgcATGTTGATCATCCCCGGGCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAg         <  1:622101/151‑1 (MQ=255)                                                                                                                                      gcATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAgg        >  1:568307/1‑151 (MQ=255)                                                                                                                                      gcATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAgg        >  1:475964/1‑151 (MQ=255)                                                                                                                                         tGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGgcg      <  1:354601/150‑1 (MQ=255)                                                                                                                                             gATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGc  >  2:937231/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                               ACCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCACCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGC  >  NC_000913/2858782‑2859071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑