Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,973,783	G→A	A27V (GCG→GTG)	lplT ←	lysophospholipid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,973,783	0	G	A	98.6%	256.6 / NA	69	A27V (GCG→GTG)	lplT	lysophospholipid transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (28/40);  minor base C (1/0);  total (29/40)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAGCTATCCGCCACCTGCCCGACAAACGGCGCAAAAAGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACGCAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAAAGTCAGGTTTGCCGCTGCCAAGTAAT  >  NC_000913/2973635‑2973930                                                                                                                                                     |                                                                                                                                                    gAAGCTATCCGCCACCTGCCCGACAAACGGCGCAAAAAGAATGTAAGCACCTACAAACACCATTGGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAAcaca                                                                                                                                                   <  2:21722/151‑1 (MQ=255)   aGCTATCCGCCACCTGCCCGACAAACGGCGCAAAAAGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACaga                                                                                                                                                 <  1:475136/151‑1 (MQ=255)       aTCCGCCACCTGCCCGACAAACGGCGCAAAAAGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACagagag                                                                                                                                              <  2:994665/150‑1 (MQ=255)       aTCCGCCACCTGCCCGACAAACGGCGCAAAAAGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAgagaga                                                                                                                                             <  1:419289/151‑1 (MQ=255)        tCCGCCACCTGCCCGACAAACGGCGCAAAAAGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACagaga                                                                                                                                               <  2:1110863/148‑1 (MQ=255)        tCCGCCACCTGCCCGACAAACGGCGCAAAAAGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAgagaga                                                                                                                                             >  1:781311/1‑150 (MQ=255)             cACCTGCCCGACAAACGGCGCAAAAAGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTgc                                                                                                                                       >  2:867935/1‑151 (MQ=255)              aCCTGCCCGACAAACGGCGCAAAAAGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTgcg                                                                                                                                      >  1:447709/1‑151 (MQ=255)                  gCCCGACAAACGGCGCAAAAAGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCAc                                                                                                                                  >  2:650478/1‑151 (MQ=255)                   cccGACAAACGGCGCAAAAAGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACg                                                                                                                                 <  2:544907/151‑1 (MQ=255)                    ccGACAAACGGCGCAAAAAGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACg                                                                                                                                 >  2:190670/1‑150 (MQ=255)                     cGACAAACGGCGCAAAAAGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGAt                                                                                                                               <  2:371540/151‑1 (MQ=255)                      gACCAACGGCGCAAAAAGGATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTAAGTAACGCCAGAGTGGCAAACAGTAGAGCATTAGCGCCAAACCCAGAGAGAAACTGCGCCACGAt                                                                                                                               >  2:648320/1‑150 (MQ=255)                      gACAAACGGCGCAAAAAGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATa                                                                                                                              <  1:577219/151‑1 (MQ=255)                          aaCGGCGCAAAAAGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCg                                                                                                                          <  1:457910/151‑1 (MQ=255)                           aCGGGGCAAAAAGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGc                                                                                                                         >  2:96940/1‑151 (MQ=255)                            cGGCGCAAAAAGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCt                                                                                                                        >  2:1008004/1‑151 (MQ=255)                                  aaaaaGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATc                                                                                                                  >  2:1123248/1‑151 (MQ=255)                                   aaaaGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATcc                                                                                                                 >  1:980446/1‑151 (MQ=255)                                    aaaGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCTCGATAACCGCTTTCATccc                                                                                                                <  2:657870/151‑1 (MQ=255)                                     aaGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATcccc                                                                                                               <  1:330426/151‑1 (MQ=255)                                        aaTGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTc                                                                                                            <  1:809919/151‑1 (MQ=255)                                           gTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGa                                                                                                          <  2:530021/150‑1 (MQ=255)                                           gTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGAc                                                                                                         <  2:263/151‑1 (MQ=255)                                            tAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGAcc                                                                                                        >  2:970004/1‑151 (MQ=255)                                               gCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACcaca                                                                                                     <  2:1188581/151‑1 (MQ=255)                                                 aCCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAAc                                                                                                   >  1:1150960/1‑151 (MQ=255)                                                   cTACCAACTCCATTTGCAGGATGGGCAGACTCCACTCCAGACAGCACTGCGCTTACAGTAGCGCAAGAGTGGCAAGCAATAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACaa                                                                                                    <  2:701474/148‑1 (MQ=255)                                                           acCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAg                                                                                         <  2:612567/151‑1 (MQ=255)                                                            ccATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAgt                                                                                        >  2:49885/1‑151 (MQ=255)                                                              aTTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAgtgt                                                                                      >  1:831103/1‑151 (MQ=255)                                                                ttGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGc                                                                                    >  2:1030977/1‑151 (MQ=255)                                                                    aGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTg                                                                                <  1:1010479/151‑1 (MQ=255)                                                                      gATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGac                                                                              <  2:1137870/151‑1 (MQ=255)                                                                      gATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGac                                                                              >  2:902885/1‑151 (MQ=255)                                                                       aTGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGact                                                                             >  1:137159/1‑151 (MQ=255)                                                                         gggCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGactca                                                                           <  2:156788/151‑1 (MQ=255)                                                                           gctggctCCTCTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCCAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCGCGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGactcaca                                                                         <  2:413087/151‑2 (MQ=255)                                                                           gctggctCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGactcact                                                                         >  2:881859/1‑151 (MQ=255)                                                                             tggctCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGactcactc                                                                        >  2:534414/1‑150 (MQ=255)                                                                               gctCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATc                                                                     <  2:544978/151‑1 (MQ=255)                                                                               gctCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATc                                                                     >  1:457790/1‑151 (MQ=255)                                                                                 tCCACTCCGTATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCgt                                                                   >  1:1053645/1‑151 (MQ=255)                                                                                    aCTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGtgtt                                                                <  2:980446/151‑1 (MQ=255)                                                                                      tCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGAACACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGtgttgt                                                              >  2:391049/1‑151 (MQ=255)                                                                                       ccGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTgttgtt                                                             >  1:900028/1‑151 (MQ=255)                                                                                        cGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTc                                                            <  2:607734/151‑1 (MQ=255)                                                                                          gATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCg                                                          <  2:506896/151‑1 (MQ=255)                                                                                            tAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCt                                                        >  1:15655/1‑151 (MQ=255)                                                                                              gAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTc                                                      <  1:650495/151‑1 (MQ=255)                                                                                                          cAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTTCCCAGCt                                          <  2:34267/151‑1 (MQ=255)                                                                                                             tAACGCAATAGTGGCAAACAGCAGGGCATTATCGCCATACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCtttt                                       >  2:1000496/1‑151 (MQ=255)                                                                                                              aaCGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTc                                      >  1:3906/1‑151 (MQ=255)                                                                                                                cGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCaa                                    <  2:1240482/151‑1 (MQ=255)                                                                                                                   cAGAGTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGt                                 <  2:299602/151‑1 (MQ=255)                                                                                                                       gTGGCAAACAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACa                             <  2:471900/151‑1 (MQ=255)                                                                                                                            aaaCAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAAAGTc                        >  2:587127/1‑151 (MQ=255)                                                                                                                             aaCAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAAAGTc                        >  2:1202760/1‑150 (MQ=255)                                                                                                                             aaCAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAAAGTCa                       <  2:368144/151‑1 (MQ=255)                                                                                                                             aaCAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAAAGTCa                       <  2:781303/151‑1 (MQ=255)                                                                                                                              aCAGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAAAGTc                        >  1:34365/1‑149 (MQ=255)                                                                                                                                aGTAGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAAAGTCAGGt                    <  1:781648/151‑1 (MQ=255)                                                                                                                                   aGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAAAGTCAGGTTTg                 <  1:819533/151‑1 (MQ=255)                                                                                                                                   aGGGCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAAAGTCAGGTTTg                 <  2:49774/151‑1 (MQ=255)                                                                                                                                      gCATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAAAGTCAGGTTTGCCg              <  2:24033/151‑1 (MQ=255)                                                                                                                                       cATTATCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAAAGTCAGGTTTGCCGc             <  1:847140/151‑1 (MQ=255)                                                                                                                                           aTCGCCAAACACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAAAGTCAGGTTTGCCGCTGc          <  2:361390/150‑1 (MQ=255)                                                                                                                                                  aaCACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAAAGTCAGGTTTGCCGCTGCCAAGTAAt  <  1:926283/151‑1 (MQ=255)                                                                                                                                                  aaCACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAAAGTCAGGTTTGCCGCTGCCAAGTAAt  <  2:900025/151‑1 (MQ=255)                                                                                                                                                  aaCACAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAAAGTCAGGTTTGCCGCTGCCAAGTAAt  <  1:502879/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GAAGCTATCCGCCACCTGCCCGACAAACGGCGCAAAAAGAATGTAAGCACCTACAAACACCATTTGCAGGATGGGCTGGCTCCACTCCGGATAGAACTGCGCTTTCAGTAACGCCAGAGTGGCAAACAGTAGGGCATTATCGCCAAACGCAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAAAGTCAGGTTTGCCGCTGCCAAGTAAT  >  NC_000913/2973635‑2973930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑