Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,346,796	T→C	H73H (CAT→CAC)	ptsN →	sugar‑specific enzyme IIA component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,346,796	0	T	C	100.0%	316.5 / NA	84	H73H (CAT→CAC)	ptsN	sugar‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (51/33);  total (51/33)

ACTGTCAGAGCAAAAAACGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCAT  >  NC_000913/3346648‑3346943                                                                                                                                                     |                                                                                                                                                    aCTGTCAGAGCAAAAAACGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCATGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACgg                                                                                                                                                   >  2:884806/1‑151 (MQ=255)   tGTCAGAGCAAAAAACGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGc                                                                                                                                                  >  2:802222/1‑150 (MQ=255)    gTCAGAGCAAAAAACGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGc                                                                                                                                                  <  2:862507/149‑1 (MQ=255)      cAGAGCAAAAAACGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCaaa                                                                                                                                               <  2:375138/150‑1 (MQ=255)       agagCAAAAAACGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACt                                                                                                                                             >  2:874376/1‑151 (MQ=255)             aaaaaCGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGga                                                                                                                                          >  2:773541/1‑148 (MQ=255)                aaCGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGgaaga                                                                                                                                       >  1:1082843/1‑148 (MQ=255)                  cgcgCCCTGGACATCATCAGCGAGCTTGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGATGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGTTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGaagaag                                                                                                                                     <  2:1065674/148‑1 (MQ=255)                   gcgcCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGC‑GGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATAct                                                                                                                                <  2:767146/151‑1 (MQ=255)                    cgcCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATAct                                                                                                                                <  1:556981/151‑1 (MQ=255)                      cccTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATActc                                                                                                                               >  2:800541/1‑150 (MQ=255)                       ccTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTg                                                                                                                             <  2:392288/151‑1 (MQ=255)                       ccTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTg                                                                                                                             <  1:140317/151‑1 (MQ=255)                          ggAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTgc                                                                                                                            <  2:517645/149‑1 (MQ=255)                          ggAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTgc                                                                                                                            <  2:63985/149‑1 (MQ=255)                           gAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTgcgcg                                                                                                                         <  1:28606/151‑1 (MQ=255)                           gAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTgc                                                                                                                            <  1:269583/148‑1 (MQ=255)                            aaaTCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTgcgcgc                                                                                                                        >  1:1029692/1‑151 (MQ=255)                             aaTCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGcc                                                                                                                       >  2:779122/1‑151 (MQ=255)                              atcatcAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCg                                                                                                                      <  1:861785/151‑1 (MQ=255)                               tcatcaGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGt                                                                                                                     >  1:696916/1‑151 (MQ=255)                                 atcaGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTg                                                                                                                   <  1:903459/151‑1 (MQ=255)                                   caGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTgg                                                                                                                  <  2:530734/150‑1 (MQ=255)                                   caGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTgg                                                                                                                  <  1:1186159/150‑1 (MQ=255)                                   caGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGc                                                                                                                 <  1:103150/151‑1 (MQ=255)                                    aGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCg                                                                                                                >  2:586504/1‑151 (MQ=255)                                     gCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGt                                                                                                               <  1:659351/151‑1 (MQ=255)                                       gAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGttt                                                                                                             >  2:512873/1‑151 (MQ=255)                                        aGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGtttt                                                                                                            <  1:941828/151‑1 (MQ=255)                                           tGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGCAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGt                                                                                                         >  2:858999/1‑151 (MQ=255)                                           tGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGt                                                                                                         >  2:856719/1‑151 (MQ=255)                                           tGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGt                                                                                                         >  1:583902/1‑151 (MQ=255)                                           tGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGt                                                                                                         >  2:1062904/1‑151 (MQ=255)                                            ggcggcGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGtt                                                                                                        >  1:821238/1‑151 (MQ=255)                                            ggcggcGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGCATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGtt                                                                                                        <  1:801491/151‑1 (MQ=255)                                              cggcgAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTc                                                                                                       >  2:638726/1‑150 (MQ=255)                                                 cgAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGc                                                                                                    >  1:729640/1‑150 (MQ=255)                                                  gAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTc                                                                                                  >  2:28369/1‑151 (MQ=255)                                                   aaaCAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCg                                                                                                 >  1:656924/1‑151 (MQ=255)                                                     acaacTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGaa                                                                                               <  1:955107/151‑1 (MQ=255)                                                      caacTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGaa                                                                                               >  2:619786/1‑150 (MQ=255)                                                       aacTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAAc                                                                                             >  2:66877/1‑151 (MQ=255)                                                           tAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGcc                                                                                          >  1:464814/1‑150 (MQ=255)                                                           tAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGcc                                                                                          <  1:857182/150‑1 (MQ=255)                                                           tAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGc                                                                                           <  2:1200607/149‑1 (MQ=255)                                                           tAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCt                                                                                         >  2:363079/1‑151 (MQ=255)                                                            aGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCt                                                                                         <  1:106890/150‑1 (MQ=255)                                                            aGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCt                                                                                         >  1:13915/1‑150 (MQ=255)                                                            aGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCACTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCt                                                                                         >  1:60235/1‑150 (MQ=255)                                                             gCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCtat                                                                                       >  1:389731/1‑151 (MQ=255)                                                              ccTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCtat                                                                                       >  1:327377/1‑150 (MQ=255)                                                                 gccgccTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCt                                                                                   >  2:1234672/1‑151 (MQ=255)                                                                 gccgccTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCt                                                                                   <  1:81363/151‑1 (MQ=255)                                                                   cgccTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCttt                                                                                 >  2:763955/1‑151 (MQ=255)                                                                   cgccTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCttt                                                                                 >  2:565457/1‑151 (MQ=255)                                                                           gtggtTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGcc                                                                          >  2:712276/1‑150 (MQ=255)                                                                           gtggtTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGcc                                                                          >  2:1023397/1‑150 (MQ=255)                                                                             ggtTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATc                                                                       >  2:1232669/1‑151 (MQ=255)                                                                                  ttGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGAca                                                                   >  2:2449/1‑150 (MQ=255)                                                                                   tGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGAcaac                                                                 >  2:1145442/1‑151 (MQ=255)                                                                                    gAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGAcaacc                                                                <  2:989077/151‑1 (MQ=255)                                                                                      aGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGAcaacca                                                               >  1:407620/1‑150 (MQ=255)                                                                                          aTCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACaaccaacc                                                            <  2:583880/149‑1 (MQ=255)                                                                                                  gcgGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGactta                                                  <  2:1004660/151‑1 (MQ=255)                                                                                                  gcgGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGactt                                                   >  2:543988/1‑150 (MQ=255)                                                                                                   cgGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGacttac                                                 >  1:642704/1‑151 (MQ=255)                                                                                                    gggAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGacttact                                                >  1:36903/1‑151 (MQ=255)                                                                                                       aaaaaaTGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTCTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACtttt                                             >  2:1129011/1‑151 (MQ=255)                                                                                                         aaaaTGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGGGGACTTACTTTTTg                                           >  2:663330/1‑151 (MQ=255)                                                                                                            aTGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGccc                                        <  1:806248/151‑1 (MQ=255)                                                                                                                gCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCctgct                                    <  1:512894/151‑1 (MQ=255)                                                                                                                     aCCGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGc                               >  1:639051/1‑151 (MQ=255)                                                                                                                       cGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCg                             >  2:12414/1‑151 (MQ=255)                                                                                                                       cGGTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCg                             <  2:117942/151‑1 (MQ=255)                                                                                                                        ggTATCGGCAATGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCgg                            >  1:902663/1‑151 (MQ=255)                                                                                                                                 aaTGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCaa                    >  1:233331/1‑150 (MQ=255)                                                                                                                                   tGGTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACt                 >  2:1084994/1‑151 (MQ=255)                                                                                                                                    ggTATTGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTa                <  2:481446/151‑1 (MQ=255)                                                                                                                                         tGCCATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAAc            >  1:895898/1‑150 (MQ=255)                                                                                                                                           ccATTCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCa         <  2:518058/151‑1 (MQ=255)                                                                                                                                               tCCGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTg     >  2:132804/1‑151 (MQ=255)                                                                                                                                                ccGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGc    <  2:119859/151‑1 (MQ=255)                                                                                                                                                 cGCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGc    >  2:224485/1‑150 (MQ=255)                                                                                                                                                  gCACGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCAt  >  2:618297/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    ACTGTCAGAGCAAAAAACGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCAT  >  NC_000913/3346648‑3346943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑