Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,356,384	T→C	S554P (TCG→CCG)	gltB →	glutamate synthase, large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,356,384	0	T	C	100.0%	249.6 / NA	66	S554P (TCG→CCG)	gltB	glutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/37);  total (29/37)

TCCGCCAGCAGTTTGCCCAGGTG‑ACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCGAAA  >  NC_000913/3356241‑3356528                                                                                                                                                 |                                                                                                                                                 ccccccAGCATTTTGCCCGGGTT‑CCAACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGcc                                                                                                                                              <  2:391696/144‑1 (MQ=255)  cccccggcatTTTCCCCGGTTG‑ACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAAccgccg                                                                                                                                             <  2:106533/142‑1 (MQ=255)  ccGCCAGCAGTTTGCCCAGGTG‑ACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGAtt                                                                                                                                          >  1:1207940/1‑151 (MQ=255)    gCCAGCAGTTTGCCCAGGTG‑ACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCt                                                                                                                                        >  1:920332/1‑151 (MQ=255)       cgcagTTTTCCCGGGTGCACAACCCCGCCAATCGACCCGCTGCGTGAAGCGCTTTTTATGTCGCTCGCCCCCGGTATCGGTCGTGAAATGACCGTCTTTTGCGAAGCAGAGGGCCAGGCGCCCCGTTTAAGCTTTAAACCGCCGATTCTGc                                                                                                                                      <  2:152198/150‑1 (MQ=255)         cagTTTGCCCAGGTG‑ACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGctc                                                                                                                                    >  1:976633/1‑150 (MQ=255)           gTTTGCCCAGGTG‑ACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTAc                                                                                                                                 >  2:333566/1‑151 (MQ=255)            tttGCCCAGGTG‑ACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACt                                                                                                                                >  2:258873/1‑151 (MQ=255)            ttcccccGGGTG‑CCAACCCCGCCAATCGCCCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGct                                                                                                                                     <  1:1105188/142‑1 (MQ=255)                 cctgggtacacaccccGCCAATCGCCCCGTTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGACCGTCTTTTTCGAAGCAGAGGCCCAGGCGCCCCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCg                                                                                                                             <  2:751890/137‑1 (MQ=255)                   aGGTG‑ACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGAttt                                                                                                                         >  1:1108575/1‑151 (MQ=255)                      tG‑ACTATCCACCCAATTGCCCCGTTGCGTGAAGCGAATGTTCGGCCGCTGGCCACCAGAATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCCGGCGCTCCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCTATTTCa                                                                                                                       <  2:1058969/150‑1 (MQ=255)                       g‑ACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCaa                                                                                                                      >  1:549051/1‑150 (MQ=255)                           tAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAAc                                                                                                                    >  1:497605/1‑149 (MQ=255)                           tAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACAGTCGATTCTGCTATACTCCGCTTTCaaa                                                                                                                     >  2:697486/1‑148 (MQ=255)                            aaCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGc                                                                                                                 >  2:1060887/1‑151 (MQ=255)                              cacGCCAATCGACCCGCTGCGTGAAGAGCATGTTATGTCGCTAGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCAGCTCTACTCCGATTTCAAACAGCTc                                                                                                               >  2:753657/3‑151 (MQ=255)                                   cAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCacgac                                                                                                          >  1:329088/1‑151 (MQ=255)                                     aTCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCacgacg                                                                                                         >  1:723067/1‑150 (MQ=255)                                       cGACCCGCTGGGTGAAGCGCATTTTATGTCGCTCGCCTCCAGTCACGTTCGTGAAATGACCGTCTTTTGCGAAGCAGAGGGCCAGCCCCGCCGTTTAACCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCacgacg                                                                                                         <  1:162607/148‑1 (MQ=255)                                        gACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCCCCAGTATCGGTCGTGAAATGAACGTTTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATg                                                                                                      <  2:352332/150‑1 (MQ=255)                                          cccGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGAt                                                                                                       <  2:588404/147‑1 (MQ=255)                                          cccGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGaaa                                                                                                   <  1:65448/151‑1 (MQ=255)                                           ccGCTGCGTAAAGCGCATGTTATGTCGCTCGCCCCCAGTATCGGTCGTAAAATGAACTTTTTTTGCAAAGCAGAGGGCCAGGCGCACAGTTAAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAag                                                                                                  <  2:1207955/151‑1 (MQ=255)                                            cGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAaga                                                                                                 <  1:1137889/151‑1 (MQ=255)                                                gCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAgagg                                                                                               <  1:341332/149‑1 (MQ=255)                                                 cGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTTTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAgaggag                                                                                             <  2:401081/150‑1 (MQ=255)                                                 cGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGc                                                                                            >  2:838339/1‑151 (MQ=255)                                                  gtaaaGCGCATGTTATGTCGCTCGCCCCCAGTATCGGTGGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCCCCTTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAgagga                                                                                              <  2:1077654/145‑1 (MQ=255)                                                        cgcATTTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTAcc                                                                                      <  1:710636/150‑1 (MQ=255)                                                         acttGTTATGTCGCTCGCCACAAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCGGGCGCACCGTTTAAGCTTTAACCCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACcg                                                                                     <  1:81081/147‑1 (MQ=255)                                                               tATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACcgcg                                                                                   <  2:827239/146‑1 (MQ=255)                                                                 tGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATAc                                                                            >  1:1078020/1‑151 (MQ=255)                                                                   tcgctcgcCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGc                                                                          >  1:18995/1‑151 (MQ=255)                                                                     gctcgcCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCt                                                                         <  1:855818/150‑1 (MQ=255)                                                                      ctcgcCCCCATAATCGGTCGTCAAATGAACCTCTTTTGCGAATCGTGGCGCCCGCCCCACCTTTTAACCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCt                                                                         <  1:931267/149‑1 (MQ=255)                                                                        cgcCACCAGTATCGGTCGTAAAATGAGCGGTTTTGGCGAAGCAGAGGGCCAGGCACACCGTTTAATCTTAAATCCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGat                                                                     <  2:437083/151‑1 (MQ=255)                                                                           caccaGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATc                                                                  <  1:145439/151‑1 (MQ=255)                                                                                 tATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCttt                                                            >  1:542600/1‑151 (MQ=255)                                                                                  aTCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCCCCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCa                                                                 <  1:330431/145‑1 (MQ=255)                                                                                        cGTGAAATGAACGTCTTTTGCGAAGAAGGGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTc                                                      <  2:749222/150‑1 (MQ=255)                                                                                         gTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCAc                                                    >  2:360580/1‑151 (MQ=255)                                                                                          tGAAATGAACGTCTTTGGCGAAGCAGAGGGCCAGGCGCCCCCTTTAAGCTTTAACCCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGt                                                       <  1:1126358/147‑1 (MQ=255)                                                                                            aaaTGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTaa                                                 <  2:1134118/151‑1 (MQ=255)                                                                                             aaTGAACTTCTTTTGCGAAGCAGAGGGCCAGGCGCGCCGTTAAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTaaa                                                <  2:407907/151‑1 (MQ=255)                                                                                                 atggTCTTTTGCGAACCAGAGGGCCAGGAGCACCGTTAAACCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAAcc                                             <  2:897329/147‑1 (MQ=255)                                                                                                 accGTCTTTTGCGAAGCAGAGGGCCAGGCGCCCCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCa                                            <  1:524027/149‑1 (MQ=255)                                                                                                    gTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGc                                         >  1:325392/1‑151 (MQ=255)                                                                                                     tCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGc                                         >  1:562652/1‑150 (MQ=255)                                                                                                      cTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTc                                       >  2:512718/1‑151 (MQ=255)                                                                                                             gAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCg                                 >  1:497695/1‑150 (MQ=255)                                                                                                             gAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCg                                 >  2:1006345/1‑150 (MQ=255)                                                                                                              aaGCAGAGGGCAAGGCGCACCGTTTAGGTTTAAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGAAATAACGCGAAGATACGCTGGATATAAAATTTAACGTCACTAAAACCACGCTc                                       <  1:1246532/143‑1 (MQ=255)                                                                                                                  agagGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTc                           >  1:966842/1‑151 (MQ=255)                                                                                                                   gagGGCCGGGCGCCCCTTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTc                           <  2:723062/150‑1 (MQ=255)                                                                                                                    agGGCCGGGCGCCCCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCa                          <  2:668894/150‑1 (MQ=255)                                                                                                                      ggCCAGGCGCCCCTTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAag                       <  1:643399/151‑1 (MQ=255)                                                                                                                         cAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGc                    >  1:602826/1‑151 (MQ=255)                                                                                                                           ggCGCACCTTTTAAGTTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCt                   <  2:4330/150‑1 (MQ=255)                                                                                                                             cgcACCATTAGAGCTTTAAACCGCCGATTCTTCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCtgt                 <  2:319197/150‑1 (MQ=255)                                                                                                                               cACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCg              >  1:182613/1‑151 (MQ=255)                                                                                                                                   gTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACa           >  2:780369/1‑150 (MQ=255)                                                                                                                                   gTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACa           >  1:1116500/1‑150 (MQ=255)                                                                                                                                     ttAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACaa          >  2:449086/1‑149 (MQ=255)                                                                                                                                       aaGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGcc      <  2:1206176/151‑1 (MQ=255)                                                                                                                                         gCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCg     <  2:346183/150‑1 (MQ=255)                                                                                                                                            ttAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCGaaa  <  1:629737/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                 TCCGCCAGCAGTTTGCCCAGGTG‑ACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCGAAA  >  NC_000913/3356241‑3356528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑