Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,400,928	C→T	A54T (GCA→ACA)	mreB ←	cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,400,928	0	C	T	100.0%	276.9 / NA	71	A54T (GCA→ACA)	mreB	cell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/30);  total (41/30)

GCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACG  >  NC_000913/3400782‑3401072                                                                                                                                                   |                                                                                                                                                 gCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCt                                                                                                                                              <  2:945213/151‑1 (MQ=255)   tGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTAc                                                                                                                                            >  2:185893/1‑151 (MQ=255)             cACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGt                                                                                                                                  >  1:1246676/1‑151 (MQ=255)              aCTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTg                                                                                                                                 >  1:620511/1‑151 (MQ=255)                  gTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAAcc                                                                                                                             <  1:529606/151‑1 (MQ=255)                   tttGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCg                                                                                                                            >  1:1108118/1‑151 (MQ=255)                    ttGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCgg                                                                                                                           >  2:1190358/1‑151 (MQ=255)                       atgaAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCAc                                                                                                                        >  1:676256/1‑151 (MQ=255)                         gaAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACg                                                                                                                       >  2:407858/1‑150 (MQ=255)                          aaGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACg                                                                                                                       >  2:472860/1‑149 (MQ=255)                             tGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCt                                                                                                                  >  2:316616/1‑151 (MQ=255)                                tGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGAc                                                                                                               >  2:290182/1‑151 (MQ=255)                                tGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGAc                                                                                                               >  2:809995/1‑151 (MQ=255)                                  gAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACg                                                                                                              >  2:482970/1‑150 (MQ=255)                                       ttttttCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGc                                                                                                        >  2:335112/1‑151 (MQ=255)                                       ttttttCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGc                                                                                                        >  2:569215/1‑151 (MQ=255)                                         ttttCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGcc                                                                                                       >  2:460745/1‑150 (MQ=255)                                          tttCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGccac                                                                                                     >  2:1230664/1‑151 (MQ=255)                                              aGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACg                                                                                                 >  2:1051075/1‑151 (MQ=255)                                              aGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACg                                                                                                 <  1:647319/151‑1 (MQ=255)                                                tCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACgg                                                                                                >  2:678632/1‑150 (MQ=255)                                                 cACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGaa                                                                                              <  2:1171358/151‑1 (MQ=255)                                                   cGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAgg                                                                                            >  2:547367/1‑151 (MQ=255)                                                    gaagaaGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGc                                                                                           >  2:112154/1‑151 (MQ=255)                                                            cGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCaa                                                                                   <  2:395235/151‑1 (MQ=255)                                                              gCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATa                                                                                 <  2:611318/151‑1 (MQ=255)                                                                gATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACg                                                                               >  2:461975/1‑151 (MQ=255)                                                                 aTAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGa                                                                              <  2:798604/151‑1 (MQ=255)                                                                   aaCGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGAt                                                                             <  1:151661/150‑1 (MQ=255)                                                                     cGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGcc                                                                          >  2:869366/1‑151 (MQ=255)                                                                      gCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCt                                                                         <  2:903327/151‑1 (MQ=255)                                                                        cGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTg                                                                       <  2:197103/151‑1 (MQ=255)                                                                          tCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTc                                                                     >  2:651145/1‑151 (MQ=255)                                                                               cATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCtttt                                                                >  2:509176/1‑151 (MQ=255)                                                                                aTTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCtttt                                                                >  1:413254/1‑150 (MQ=255)                                                                                 ttGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTa                                                               >  1:899914/1‑150 (MQ=255)                                                                                         aTGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATg                                                       >  1:340108/1‑150 (MQ=255)                                                                                                caATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTAtt                                               <  1:1068610/151‑1 (MQ=255)                                                                                                caATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTAtt                                               <  1:409154/151‑1 (MQ=255)                                                                                                 aaTATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTc                                              >  2:836530/1‑151 (MQ=255)                                                                                                 aaTATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTc                                              <  2:342628/151‑1 (MQ=255)                                                                                                       tcctggCGTACGGCCCAGCATCTGCTTCGCGGCATGACCTACTGTAGCTATGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCa                                           >  2:457407/5‑148 (MQ=255)                                                                                                       gCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTa                                        <  2:995918/151‑1 (MQ=255)                                                                                                           ggCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCa                                    <  2:1110988/151‑1 (MQ=255)                                                                                                           ggCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCa                                    <  1:273696/151‑1 (MQ=255)                                                                                                                aCGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTc                                <  2:686276/150‑1 (MQ=255)                                                                                                                aCGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTc                                <  1:399847/150‑1 (MQ=255)                                                                                                                aCGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCa                               <  1:487076/151‑1 (MQ=255)                                                                                                                  ggCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCaa                              >  1:874810/1‑150 (MQ=255)                                                                                                                  ggCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAAt                             >  1:202694/1‑151 (MQ=255)                                                                                                                    cccAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATgg                           >  2:667019/1‑151 (MQ=255)                                                                                                                         cATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAg                      <  1:109937/151‑1 (MQ=255)                                                                                                                            cTGCTTAGCGTCATGACCTACTGTAGCTACGCTTTTAGGCGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTTCTTTTACATAAATGAGGGTATTCGCAGTACCAAGGTCAATGGACa                        >  2:1233556/1‑146 (MQ=255)                                                                                                                              gCTTCGCGTCATGACCTACTGTAGCTTCGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTTAATGGACAAGTCAt                  <  2:1032413/150‑1 (MQ=255)                                                                                                                              gCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCa                   <  2:972602/149‑1 (MQ=255)                                                                                                                                ttCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCa                   <  2:896369/147‑1 (MQ=255)                                                                                                                                ttCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTgg               >  2:1147766/1‑151 (MQ=255)                                                                                                                                 tCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTgg               <  2:1226563/150‑1 (MQ=255)                                                                                                                                 tCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTgg               >  1:1244002/1‑150 (MQ=255)                                                                                                                                 tCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTgg               <  2:829735/150‑1 (MQ=255)                                                                                                                                  cgcgTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGa              <  1:1214205/150‑1 (MQ=255)                                                                                                                                   gcgTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGaaa            <  1:1190344/151‑1 (MQ=255)                                                                                                                                       cATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACAt        >  2:1241309/1‑151 (MQ=255)                                                                                                                                        aTGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATg       >  2:150103/1‑151 (MQ=255)                                                                                                                                           aCCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGcc     >  2:753938/1‑150 (MQ=255)                                                                                                                                           aCCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGc      <  2:1032899/149‑1 (MQ=255)                                                                                                                                             cTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACg  >  1:19562/1‑151 (MQ=255)                                                                                                                                             cTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACg  >  1:205549/1‑151 (MQ=255)                                                                                                                                              tACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACg  >  1:1041773/1‑150 (MQ=255)                                                                                                                                               aCTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACg  <  1:185880/149‑1 (MQ=255)                                                                                                                                               aCTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACg  <  2:532433/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 GCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACG  >  NC_000913/3400782‑3401072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑