Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,404,872	T→C	pseudogene (141/300 nt)	yrdE →	pseudogene, yedZ paralog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,404,872	0	T	C	100.0%	307.7 / NA	77	pseudogene (141/300 nt)	yrdE	pseudogene, yedZ paralog
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/30);  total (47/30)

TTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAA  >  NC_000913/3404729‑3405015                                                                                                                                                |                                                                                                                                                ttCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATcccc                                                                                                                                          >  2:909564/1‑151 (MQ=255)     ggTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCa                                                                                                                                      <  1:73410/151‑1 (MQ=255)       tACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAAc                                                                                                                                    >  2:377272/1‑151 (MQ=255)         ccATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCg                                                                                                                                  >  2:186814/1‑151 (MQ=255)          cATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGc                                                                                                                                 >  2:278247/1‑151 (MQ=255)           aTGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCt                                                                                                                                <  1:173546/151‑1 (MQ=255)             ggggTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGc                                                                                                                              >  2:451992/1‑151 (MQ=255)                gTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTa                                                                                                                           <  1:501282/151‑1 (MQ=255)                  gAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTaa                                                                                                                          <  1:948687/150‑1 (MQ=255)                  gAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTATGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAAt                                                                                                                         >  2:456066/1‑151 (MQ=255)                   aataatCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATg                                                                                                                        >  2:724008/1‑151 (MQ=255)                    ataatCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATgt                                                                                                                       <  1:138659/151‑1 (MQ=255)                    ataatCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATgt                                                                                                                       <  2:632184/151‑1 (MQ=255)                       atCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATgtcgt                                                                                                                    >  1:1237549/1‑151 (MQ=255)                       atCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATgtcgt                                                                                                                    >  1:22070/1‑151 (MQ=255)                       atCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATgtcgt                                                                                                                    >  2:271684/1‑151 (MQ=255)                           gATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTAt                                                                                                                <  1:1211580/151‑1 (MQ=255)                            aTTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATc                                                                                                               >  2:1115308/1‑151 (MQ=255)                              tttgtttgACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCtt                                                                                                             >  1:335049/1‑151 (MQ=255)                              tttgtttgACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCt                                                                                                              <  1:820545/150‑1 (MQ=255)                               ttgtttgACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTg                                                                                                            >  1:128457/1‑151 (MQ=255)                                 gtttgACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGt                                                                                                           <  2:831868/150‑1 (MQ=255)                                 gtttgACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTc                                                                                                          <  1:48465/151‑1 (MQ=255)                                 gtttgACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTc                                                                                                          <  1:159905/151‑1 (MQ=255)                                  tttgACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTcg                                                                                                         >  1:518409/1‑151 (MQ=255)                                     gACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTcgcg                                                                                                       >  2:700556/1‑150 (MQ=255)                                         aCAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATcc                                                                                                   <  1:432430/150‑1 (MQ=255)                                           catttAATCAACCGAACCTATTTAATGCTGCGCATTGTCTATCGGTTAATTTTGCGTGCTTTACCCTTCCCATCTATTCGTACGATGTAGCGTAATTTGGCCAATCTCCAGCAACCTCTACGTAATGACGTCTATCTTGCCGCGGTCCt                                                                                                  >  2:461724/4‑149 (MQ=255)                                           aaaTTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTg                                                                                                 >  2:629171/1‑150 (MQ=255)                                           aaaTTAATCACTCGAACCTATTTAATCCTGAGCATTGACAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGCCGAAGCAGTGCAATTTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTgg                                                                                                >  2:545270/1‑151 (MQ=255)                                            aaTTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGc                                                                                               >  1:315346/1‑151 (MQ=255)                                              ttAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCAt                                                                                             >  1:1033937/1‑151 (MQ=255)                                                aaTCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATc                                                                                            <  1:957738/150‑1 (MQ=255)                                                 aTCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                                                                                          >  1:1029861/1‑151 (MQ=255)                                                   cACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCt                                                                                         >  2:400721/1‑150 (MQ=255)                                                   cACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTa                                                                                        <  1:303763/151‑1 (MQ=255)                                                    aCTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTAc                                                                                       >  2:5314/1‑151 (MQ=255)                                                    aCTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTAc                                                                                       >  1:99253/1‑151 (MQ=255)                                                        gAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACatt                                                                                    >  2:1030353/1‑150 (MQ=255)                                                        gAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACatt                                                                                    >  2:798503/1‑150 (MQ=255)                                                             tATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTtg                                                                               >  1:186633/1‑150 (MQ=255)                                                              aTTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTtgtg                                                                             >  2:1068595/1‑151 (MQ=255)                                                                   aTGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtg                                                                        <  1:1118670/151‑1 (MQ=255)                                                                    tGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgt                                                                       >  1:526507/1‑151 (MQ=255)                                                                          gCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGa                                                                     >  2:411537/1‑147 (MQ=255)                                                                          gCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAt                                                                 <  1:786142/151‑1 (MQ=255)                                                                           cATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAtt                                                                >  1:80752/1‑151 (MQ=255)                                                                            aTTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAtt                                                                >  2:650734/1‑150 (MQ=255)                                                                            aTTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAtt                                                                >  2:784588/1‑150 (MQ=255)                                                                              tGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtc                                                             >  1:797966/1‑151 (MQ=255)                                                                               gTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtc                                                             <  2:954148/150‑1 (MQ=255)                                                                                  aaTCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCAt                                                         >  2:383627/1‑151 (MQ=255)                                                                                  aaTCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCAt                                                         >  2:1178740/1‑151 (MQ=255)                                                                                   aTCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCAtt                                                        <  2:610798/151‑1 (MQ=255)                                                                                      ggTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCa                                                     <  1:871958/151‑1 (MQ=255)                                                                                           aTTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGcccc                                                >  1:325824/1‑151 (MQ=255)                                                                                                  gTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCtt                                         <  2:391865/151‑1 (MQ=255)                                                                                                  gTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCtt                                         <  2:335036/151‑1 (MQ=255)                                                                                                  gTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCtt                                         <  1:916185/151‑1 (MQ=255)                                                                                                    gCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTc                                        <  1:1181266/150‑1 (MQ=255)                                                                                                    gCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCg                                       <  2:62798/151‑1 (MQ=255)                                                                                                       ttAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGc                                      >  2:49672/1‑149 (MQ=255)                                                                                                       ttAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGc                                      <  1:271677/149‑1 (MQ=255)                                                                                                           cATTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggct                                >  2:806929/1‑151 (MQ=255)                                                                                                            aTTCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggctg                               >  2:313920/1‑151 (MQ=255)                                                                                                             ttCACATCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggctgg                              >  1:229802/1‑151 (MQ=255)                                                                                                               cacaTCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggctggct                            >  2:803175/1‑151 (MQ=255)                                                                                                                   tCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttc                        >  1:474468/1‑151 (MQ=255)                                                                                                                   tCTATCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttc                        >  1:1213229/1‑151 (MQ=255)                                                                                                                      aTCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagc                     >  2:815437/1‑151 (MQ=255)                                                                                                                      aTCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttca                       <  2:1160313/149‑1 (MQ=255)                                                                                                                      aTCCAGACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttc                        <  1:724013/148‑1 (MQ=255)                                                                                                                           gACGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagcttttc                <  2:1033939/151‑1 (MQ=255)                                                                                                                             cGATGCAGTGAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCAc              >  1:487975/1‑151 (MQ=255)                                                                                                                                      gAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGtt      >  2:738772/1‑150 (MQ=255)                                                                                                                                      gAAAATTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGtt      <  1:909563/150‑1 (MQ=255)                                                                                                                                         aaTTGGGCAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaa  <  2:1172369/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                TTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAA  >  NC_000913/3404729‑3405015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑