Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,462,301 A→G T110A (ACG→GCG)  gspI → general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,462,3010AG100.0% 350.4 / NA 96T110A (ACG→GCG) gspIgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/61);  total (35/61)

CGGCGAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTC  >  NC_000913/3462153‑3462447
                                                                                                                                                    |                                                                                                                                                  
cGGCGAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGcg                                                                                                                                                  <  1:788796/151‑1 (MQ=255)
cGGCGAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGcg                                                                                                                                                  <  2:135148/151‑1 (MQ=255)
cGGCGAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGcg                                                                                                                                                  >  2:327413/1‑151 (MQ=255)
 ggCGAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTgcgc                                                                                                                                                 <  2:754577/151‑1 (MQ=255)
   cGAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGt                                                                                                                                               >  1:821022/1‑151 (MQ=255)
    gAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGtt                                                                                                                                              >  1:90748/1‑151 (MQ=255)
    gAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGtt                                                                                                                                              <  2:315135/151‑1 (MQ=255)
        aaaaCACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCa                                                                                                                                          <  2:45678/151‑1 (MQ=255)
          aaCACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCaa                                                                                                                                         >  2:736482/1‑150 (MQ=255)
             acGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCa                                                                                                                                     <  1:413006/151‑1 (MQ=255)
               gTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATc                                                                                                                                   >  2:420869/1‑151 (MQ=255)
                 ccAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCg                                                                                                                                  <  1:590836/150‑1 (MQ=255)
                  cAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGat                                                                                                                                >  1:173514/1‑151 (MQ=255)
                   aGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGata                                                                                                                               <  1:793100/151‑1 (MQ=255)
                   aGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGat                                                                                                                                >  2:1128655/1‑150 (MQ=255)
                    gTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGata                                                                                                                               >  1:1162062/1‑150 (MQ=255)
                      tCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGataat                                                                                                                             <  2:1124817/150‑1 (MQ=255)
                      tCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGataat                                                                                                                             <  2:338748/150‑1 (MQ=255)
                         ggCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGataata                                                                                                                            <  1:1116614/148‑1 (MQ=255)
                         ggCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGataata                                                                                                                            <  2:283340/148‑1 (MQ=255)
                         ggCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGAtaataa                                                                                                                           <  2:479019/149‑1 (MQ=255)
                         ggCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAAt                                                                                                                         <  2:794998/151‑1 (MQ=255)
                           cAAAGAGTTAATAAATGGCGGAGAGTGGATCTGGCTCAGCGATATCCACTCAAGCAAAGGCGGCAAGCTTCTGGAACGCAACATAACGGTGACATTGACCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGataat                                                                                                                             <  2:787041/145‑1 (MQ=255)
                           cAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATcc                                                                                                                       <  1:754258/151‑1 (MQ=255)
                            aaaGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCAt                                                                                                                                    <  2:568195/137‑1 (MQ=255)
                            aaaGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCAt                                                                                                                                    >  1:568221/1‑137 (MQ=255)
                            aaaGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATAAAAGCATCGATAATAAATCCg                                                                                                                      >  2:1014824/1‑151 (MQ=255)
                             aaGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCgg                                                                                                                     <  2:776148/151‑1 (MQ=255)
                                agTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGggc                                                                                                                   >  2:586939/1‑150 (MQ=255)
                                 gTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGggcag                                                                                                                 <  2:974439/151‑1 (MQ=255)
                                   taataaATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGggcagg                                                                                                                <  2:770193/150‑1 (MQ=255)
                                   taataaATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGggcag                                                                                                                 <  2:477102/149‑1 (MQ=255)
                                    aataaATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGggcaggca                                                                                                              <  1:127839/151‑1 (MQ=255)
                                    aataaATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGATATCAAAGCATCGATAATAAATCCGggcaggc                                                                                                               >  1:899351/1‑150 (MQ=255)
                                       aaaTGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGcaca                                                                                                            >  1:184486/1‑150 (MQ=255)
                                          tGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGcaca                                                                                                            >  2:498261/1‑147 (MQ=255)
                                           ggCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAgatg                                                                                                       <  1:157524/151‑1 (MQ=255)
                                             cGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAgatgat                                                                                                     >  2:243400/1‑151 (MQ=255)
                                             cGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAgatgat                                                                                                     >  2:980061/1‑151 (MQ=255)
                                              gAAGAGTGGAACTGGCGCAGCTATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGAtt                                                                                                    <  2:1062285/151‑1 (MQ=255)
                                                agagTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTaa                                                                                                  <  1:703094/151‑1 (MQ=255)
                                                  agTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAAt                                                                                                 <  1:928343/150‑1 (MQ=255)
                                                  agTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATc                                                                                                <  1:314699/151‑1 (MQ=255)
                                                  agTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATc                                                                                                <  1:1208175/151‑1 (MQ=255)
                                                        aCTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCcag                                                                                           >  1:749802/1‑150 (MQ=255)
                                                          tGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCcagc                                                                                          >  1:1067522/1‑149 (MQ=255)
                                                             cgcAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGt                                                                                     >  1:1156323/1‑151 (MQ=255)
                                                                aGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTc                                                                                  <  1:771701/151‑1 (MQ=255)
                                                                 gCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTca                                                                                 <  2:479318/151‑1 (MQ=255)
                                                                 gCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTc                                                                                  >  2:189089/1‑150 (MQ=255)
                                                                 gCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTc                                                                                  >  1:1131270/1‑150 (MQ=255)
                                                                         cACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTg                                                                         <  2:484947/151‑1 (MQ=255)
                                                                           cTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTgg                                                                        >  2:635851/1‑150 (MQ=255)
                                                                             cAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGa                                                                       <  2:1167633/149‑1 (MQ=255)
                                                                             cAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAgg                                                                     <  1:377327/151‑1 (MQ=255)
                                                                                       cGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATggcggc                                                           <  1:694593/151‑1 (MQ=255)
                                                                                         gCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGgcggcg                                                          >  2:232221/1‑150 (MQ=255)
                                                                                         gCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGgcgc                                                         >  1:483075/1‑151 (MQ=255)
                                                                                           aCGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCt                                                        <  1:1186020/150‑1 (MQ=255)
                                                                                             gCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGc                                                     <  1:815507/151‑1 (MQ=255)
                                                                                              cTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCg                                                    >  2:586493/1‑151 (MQ=255)
                                                                                                 cTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGc                                                     <  2:122669/147‑1 (MQ=255)
                                                                                                   ggAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGAt                                                  <  1:1139086/148‑1 (MQ=255)
                                                                                                   ggAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGAt                                                  <  1:969166/148‑1 (MQ=255)
                                                                                                    caaCGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTtc                                              >  1:24056/2‑151 (MQ=255)
                                                                                                     aaCGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTtct                                             >  1:692110/1‑151 (MQ=255)
                                                                                                            cATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCt                                      <  1:82435/151‑1 (MQ=255)
                                                                                                               aaCGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGGGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGTCGGCGCTGGCGATTTTCTCAATg                                        >  1:196395/1‑146 (MQ=255)
                                                                                                                aCGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAg                                   >  2:59234/1‑150 (MQ=255)
                                                                                                                  ggTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGt                                <  1:563918/151‑1 (MQ=255)
                                                                                                                  ggTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGt                                <  2:184498/151‑1 (MQ=255)
                                                                                                                        aTTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTgg                           <  2:453988/150‑1 (MQ=255)
                                                                                                                        aTTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGc                          <  2:865767/151‑1 (MQ=255)
                                                                                                                           aCCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGtt                       <  2:666039/151‑1 (MQ=255)
                                                                                                                           aCCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGt                        <  2:96209/150‑1 (MQ=255)
                                                                                                                            cccAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCg                         <  1:3776/148‑1 (MQ=255)
                                                                                                                             ccAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATACATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGtt                       >  2:295402/1‑149 (MQ=255)
                                                                                                                              cAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTAt                    <  1:327425/151‑1 (MQ=255)
                                                                                                                              cAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTAt                    <  2:1162080/151‑1 (MQ=255)
                                                                                                                              cAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTAt                    <  2:6376/151‑1 (MQ=255)
                                                                                                                               aGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTCTATg                   <  1:589337/151‑1 (MQ=255)
                                                                                                                                gCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATg                   >  1:801507/1‑150 (MQ=255)
                                                                                                                                     cAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGAttttt             >  1:159415/1‑151 (MQ=255)
                                                                                                                                     cAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGAttttt             <  1:885388/151‑1 (MQ=255)
                                                                                                                                     cAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGAtttt              <  2:419968/150‑1 (MQ=255)
                                                                                                                                     cAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGAtttt              <  2:100618/150‑1 (MQ=255)
                                                                                                                                      aGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGAtttttt            <  2:1213397/151‑1 (MQ=255)
                                                                                                                                        aCAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTc           <  1:1073172/150‑1 (MQ=255)
                                                                                                                                          aaCCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGc         >  2:430865/1‑150 (MQ=255)
                                                                                                                                           aCCTCGCTTGCGCGTTATCAAAGCATCGAAAATAAATCCGGGCAGGCACAAGATGATTAATAGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGCTTTTCTCAAAGCTTAGCGGGCACGCGTTTATGATTTTTTTGc         >  2:1109678/1‑149 (MQ=255)
                                                                                                                                            ccTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAg      <  1:19215/151‑1 (MQ=255)
                                                                                                                                             cTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCaa       <  2:821016/149‑1 (MQ=255)
                                                                                                                                             cTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAg      <  1:394973/150‑1 (MQ=255)
                                                                                                                                               cGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCtt   <  1:158901/151‑1 (MQ=255)
                                                                                                                                               cGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCtt   >  2:523678/1‑151 (MQ=255)
                                                                                                                                                gCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTc  <  1:145981/151‑1 (MQ=255)
                                                                                                                                                gCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTc  <  2:1140700/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGGCGAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTC  >  NC_000913/3462153‑3462447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑