Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,486,190 A→G Y24C (TAC→TGC)  crp → cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,486,1900AG98.7% 278.6 / ‑4.1 76Y24C (TAC→TGC) crpcAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (37/37);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

AAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCG  >  NC_000913/3486045‑3486336
                                                                                                                                                 |                                                                                                                                                  
aaGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCAt                                                                                                                                               >  1:6108/1‑151 (MQ=255)
 aGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATc                                                                                                                                              >  2:70495/1‑151 (MQ=255)
  gCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATcc                                                                                                                                             <  1:973339/151‑1 (MQ=255)
             cAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCt                                                                                                                                  >  2:324292/1‑151 (MQ=255)
             cAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCt                                                                                                                                  <  1:439630/151‑1 (MQ=255)
               aTCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCtt                                                                                                                                 >  2:375015/1‑150 (MQ=255)
                     agaCAGCGGCTTTACCTGGCTCTGGAGAACGCTTATAACAGGGGATAGCCGCCCATGGTGCTTGGAAAACCTCAAACAGCCCCGACTCTCGAATGGTTGTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTc                                                                                                                             <  2:944528/148‑1 (MQ=255)
                          gcggcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGt                                                                                                                     <  2:399908/151‑1 (MQ=255)
                            ggcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTg                                                                                                                    <  2:349661/150‑1 (MQ=255)
                             gcgTTATCTGGCTAGGGAGAAAGTTTATAACAGAGAATATCCGGGACTGTTGCTTGGAAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCAGTGCGACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCGCCAGGGTGa                                                                                                                   <  2:288611/150‑1 (MQ=255)
                              cgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGaaa                                                                                                                 <  2:1216540/151‑1 (MQ=255)
                               gTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaa                                                                                                                <  1:580040/151‑1 (MQ=255)
                                ttATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaa                                                                                                                >  2:730672/1‑150 (MQ=255)
                                 tATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaa                                                                                                                >  2:136231/1‑149 (MQ=255)
                                 tATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaa                                                                                                                >  1:577682/1‑149 (MQ=255)
                                  aTCTGGCTCTGGAGAAAGCTTATAACAGAGGATACCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAg                                                                                                              <  1:679725/150‑1 (MQ=255)
                                  aTCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAg                                                                                                              >  2:163658/1‑150 (MQ=255)
                                        ctctGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAAc                                                                                                       >  1:990502/1‑151 (MQ=255)
                                            ggAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCt                                                                                                    <  1:781475/150‑1 (MQ=255)
                                                aaaGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGtac                                                                                                <  1:1010799/150‑1 (MQ=255)
                                                      tataACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACAt                                                                                           <  1:968889/149‑1 (MQ=255)
                                                      tataACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTAATAAGCGCCCATCCACGAGCACGCTTATTCACCAGGGTGAGAAATCGGAAACGCTGTACTACATCg                                                                                         >  2:1040841/1‑151 (MQ=255)
                                                       ataACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGt                                                                                        >  1:333209/1‑151 (MQ=255)
                                                        taACAGAGGATAACCGCGCATGGTGCATGGCAAACCGCCAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAACAAGCGGAAACGCTGTACTACTTCGtt                                                                                       >  2:515189/1‑151 (MQ=255)
                                                          aCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCGACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTa                                                                                      >  1:427966/1‑150 (MQ=255)
                                                          aCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTa                                                                                      >  2:504082/1‑150 (MQ=255)
                                                          aCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTa                                                                                      >  2:81655/1‑150 (MQ=255)
                                                           cAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCACATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaa                                                                                     >  2:673566/1‑150 (MQ=255)
                                                                 aTAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctct                                                                              >  2:375981/1‑151 (MQ=255)
                                                                    aCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgtg                                                                           >  1:1218061/1‑151 (MQ=255)
                                                                     ccGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgtg                                                                           <  1:727436/150‑1 (MQ=255)
                                                                      cgcgCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGc                                                                         >  2:441675/1‑151 (MQ=255)
                                                                        cgcATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGc                                                                         >  2:898628/1‑149 (MQ=255)
                                                                        cgcATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGc                                                                         >  2:53603/1‑149 (MQ=255)
                                                                          cATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTg                                                                     >  1:792163/1‑151 (MQ=255)
                                                                           aTGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTg                                                                     >  2:248608/1‑150 (MQ=255)
                                                                           aTGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGc                                                                    >  1:892511/1‑151 (MQ=255)
                                                                             ggTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCt                                                                   <  2:902741/150‑1 (MQ=255)
                                                                                   tGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCa                                                              <  2:424241/149‑1 (MQ=255)
                                                                                    ggCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCaaa                                                            <  2:476803/150‑1 (MQ=255)
                                                                                    ggCAAACCGAAAACAGTCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAATTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCaaa                                                            <  2:403433/150‑1 (MQ=255)
                                                                                      cAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGAc                                                         <  1:233876/151‑1 (MQ=255)
                                                                                       aaaCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACg                                                        <  2:109672/151‑1 (MQ=255)
                                                                                            gCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAag                                                     >  2:497252/1‑149 (MQ=255)
                                                                                             cAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAagag                                                   <  1:728284/150‑1 (MQ=255)
                                                                                              aaaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAggg                                                 >  1:954451/1‑151 (MQ=255)
                                                                                               aaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGt                                                <  1:204940/151‑1 (MQ=255)
                                                                                                aCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTa                                               <  1:552362/151‑1 (MQ=255)
                                                                                                 cAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTaa                                              <  1:183898/151‑1 (MQ=255)
                                                                                                 cAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTa                                               >  2:955306/1‑150 (MQ=255)
                                                                                                 cAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTa                                               >  1:686947/1‑150 (MQ=255)
                                                                                                  aGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTaaa                                             <  1:1042057/151‑1 (MQ=255)
                                                                                                  aGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTaaa                                             <  1:309170/151‑1 (MQ=255)
                                                                                                     cccGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGaa                                          <  1:307984/151‑1 (MQ=255)
                                                                                                         aCTATCGAATGGTTCTTGTCACATTGCCCCATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCAGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAg                                            <  2:665478/145‑1 (MQ=255)
                                                                                                            ctcGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGAt                                     <  2:879273/149‑1 (MQ=255)
                                                                                                              cGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATcc                                   <  1:4892/149‑1 (MQ=255)
                                                                                                              cGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCctc                                 <  2:124660/151‑1 (MQ=255)
                                                                                                              cGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCct                                  <  1:783429/150‑1 (MQ=255)
                                                                                                               gAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCctct                                >  1:552400/1‑151 (MQ=255)
                                                                                                               gAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCctct                                <  2:404833/151‑1 (MQ=255)
                                                                                                                   ggTTCTTGTCTCATTGCCACATTCATAAGGTCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTTTCCTa                            <  2:1065147/151‑1 (MQ=255)
                                                                                                                    gTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTAt                           >  2:1066603/1‑151 (MQ=255)
                                                                                                                     ttCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATc                          >  1:1161822/1‑151 (MQ=255)
                                                                                                                      tCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATc                          <  2:1134603/150‑1 (MQ=255)
                                                                                                                      tCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTG‑AAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTg                        >  2:785597/1‑151 (MQ=255)
                                                                                                                        ttGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTg                        >  2:858380/1‑150 (MQ=255)
                                                                                                                         tGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCt                         <  1:1198348/148‑1 (MQ=255)
                                                                                                                                  gCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTg              <  1:373752/150‑1 (MQ=255)
                                                                                                                                   ccACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGAt            <  1:164683/151‑1 (MQ=255)
                                                                                                                                      caTTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGAtttt         <  2:577657/151‑1 (MQ=255)
                                                                                                                                       aTTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTa        <  2:141539/151‑1 (MQ=255)
                                                                                                                                        ttCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTAt       >  1:351088/1‑151 (MQ=255)
                                                                                                                                           aTAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTgg    >  1:1043094/1‑151 (MQ=255)
                                                                                                                                            tAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGc   >  2:495420/1‑151 (MQ=255)
                                                                                                                                             aaGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCg  >  2:672060/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
AAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCG  >  NC_000913/3486045‑3486336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑