Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,497,615	T→C	F205L (TTC→CTC)	nirC →	nitrite transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,497,615	0	T	C	100.0%	198.9 / NA	56	F205L (TTC→CTC)	nirC	nitrite transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/23);  total (33/23)

CAAAGGTGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGTTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATGCTACGCCGAAAGCG  >  NC_000913/3497467‑3497758                                                                                                                                                     |                                                                                                                                                cAAAGGTGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTc                                                                                                                                               <  2:620293/151‑1 (MQ=255)       tGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGctc                                                                                                                                         >  2:1197092/1‑151 (MQ=255)       tGCATTGGGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCGGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGct                                                                                                                                          >  2:488677/1‑150 (MQ=255)         cATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCtctc                                                                                                                                       >  1:1048716/1‑151 (MQ=255)         cATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCtctc                                                                                                                                       >  1:1156697/1‑151 (MQ=255)          aTTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTcc                                                                                                                                      >  1:1028190/1‑151 (MQ=255)          aTTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTcc                                                                                                                                      >  2:538144/1‑151 (MQ=255)             gtgtAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTgg                                                                                                                                   <  2:133935/151‑1 (MQ=255)                tAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTc                                                                                                                                >  2:571108/1‑151 (MQ=255)                tAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTc                                                                                                                                >  1:95558/1‑151 (MQ=255)                 aaCTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCg                                                                                                                               >  2:427148/1‑151 (MQ=255)                         ggTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACcac                                                                                                                       <  2:393862/151‑1 (MQ=255)                                 tGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGc                                                                                                               >  2:1192303/1‑151 (MQ=255)                                   gCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCt                                                                                                             <  2:327611/151‑1 (MQ=255)                                        cTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACg                                                                                                        >  2:997214/1‑151 (MQ=255)                                         tGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGc                                                                                                       >  1:165006/1‑151 (MQ=255)                                          ggATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTacac                                                                                                         <  1:1197078/148‑1 (MQ=255)                                              ggCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCg                                                                                                  <  1:789830/151‑1 (MQ=255)                                               gcgcTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCgg                                                                                                 <  2:102154/151‑1 (MQ=255)                                                     cgcACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTagt                                                                                            >  2:901518/1‑148 (MQ=255)                                                     cgcACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGAtt                                                                                            >  1:680419/1‑150 (MQ=255)                                                     cgcACTGAAGGGGCGGCGAAAGTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGATAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTAtt                                                                                            <  2:640030/150‑1 (MQ=255)                                                       cACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCggg                                                                                                >  2:656726/1‑144 (MQ=255)                                                               gggCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATaa                                                                                    >  1:1243163/1‑148 (MQ=255)                                                                  cggcgAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACctgct                                                                              <  2:1077902/151‑1 (MQ=255)                                                                      gAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTgg                                                                          >  2:199414/1‑151 (MQ=255)                                                                          tttATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAACCTGCTGTggggg                                                                       >  1:1092353/1‑148 (MQ=255)                                                                               cGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCt                                                                  <  1:30285/150‑1 (MQ=255)                                                                                gCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTAATGGTGCTAACCCGCTGgggggggaggcgg                                                                >  2:242409/1‑142 (MQ=255)                                                                                gCTAGCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTgg                                                                <  2:824542/151‑1 (MQ=255)                                                                                 cTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAACCTGCTGTGGGTGACGCTggg                                                               >  2:453495/1‑151 (MQ=255)                                                                                   aTCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTTGTCATAACCTGCTGTGGGTGACGCTggg                                                               >  2:518106/1‑149 (MQ=255)                                                                                     cTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGGCATAACCTTCTGTGGGGGGCGCTGGGTaa                                                            >  2:513239/1‑150 (MQ=255)                                                                                         tggtgTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGGGACGCTGGGGAATACtt                                                       >  1:442529/1‑151 (MQ=255)                                                                                               ctgctgGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCAAAGCCTACACGCTGGCGGGTATTTGTCATAACCCGCTGTGGGGGGAGCTGGGTAATACctttt                                                    >  2:1237688/1‑146 (MQ=255)                                                                                                 gctgGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAg                                                 <  1:319768/149‑1 (MQ=255)                                                                                                   tgGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAACCTGCTGTGGGGGACGCTGGGTAATACTTTATCAgg                                                >  2:587810/1‑148 (MQ=255)                                                                                                       aTTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAACCTGCTGTGGGGGACGCTGGGTAATACTTTATCAGGGGCCGt                                          >  1:711670/1‑150 (MQ=255)                                                                                                           aTCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCa                                     <  1:101378/151‑1 (MQ=255)                                                                                                              gcgTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATgg                                  <  2:616658/151‑1 (MQ=255)                                                                                                                  ccGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTTGTCCTAACCTGCTGTGGGGGACGCTGGGTAATACTTTATTAGGTGCCGTATTTATggg                                 >  2:1165086/1‑148 (MQ=255)                                                                                                                   cGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTg                             <  1:998757/151‑1 (MQ=255)                                                                                                                      cTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGAATGGGTCTAACCTGCCGTGGGGGACGCTCGGGAATACTTTATTAAGGGCCGTATTCCttgggttggg                           >  2:1133089/1‑150 (MQ=255)                                                                                                                        aCGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGAATTGTCATAAACTGCCATTGGGGACGCCGGGAAATACTTTATCCTGAGCCCGATTTTTGGGATTggg                           >  2:150554/1‑148 (MQ=255)                                                                                                                              aCTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTa                  <  1:250354/151‑1 (MQ=255)                                                                                                                                 ctATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTAt                 <  1:427161/149‑1 (MQ=255)                                                                                                                                  tATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGGCGCTGGGGAATACTTTATCAGGTGCCGGATTCCTGGGGTTGGGGTATTgg                    >  2:439160/1‑145 (MQ=255)                                                                                                                                   aTCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGGTTGGGTTATTGGTATGCt              >  1:327420/1‑150 (MQ=255)                                                                                                                                   aTCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATg                <  1:814883/148‑1 (MQ=255)                                                                                                                                   aTCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATGCTa             <  1:945652/151‑1 (MQ=255)                                                                                                                                     cGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATGCTAc            <  1:89601/150‑1 (MQ=255)                                                                                                                                      gCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAACCTGCTGTGGGGGACGCTGGGGAATACTTTATCAGGTGCCGTATTCATGGGGTTGGGGTATTGGTATGCTACGc          >  1:970177/1‑151 (MQ=255)                                                                                                                                         aaCATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATGCTACGCCGa       <  2:412309/151‑1 (MQ=255)                                                                                                                                         aaCATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTTGTCATAACCTGCTGTGGGTGACGCTGGGGAATACTTTATCAGGTGCCGTATTCCTGGGGTTGGGGTATTGGTATGCTACGcc         >  2:243096/1‑149 (MQ=255)                                                                                                                                            aTGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATGCTACGCCGAAAg    <  2:356390/151‑1 (MQ=255)                                                                                                                                              gACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATGCTACGCCGAAAGCg  <  1:1213231/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                CAAAGGTGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGTTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATGCTACGCCGAAAGCG  >  NC_000913/3497467‑3497758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑