Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,505,884	A→G	L46P (CTG→CCG)	yhfS ←	FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,505,884	0	A	G	100.0%	257.6 / NA	72	L46P (CTG→CCG)	yhfS	FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/36);  total (36/36)

CAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTT  >  NC_000913/3505739‑3506029                                                                                                                                                  |                                                                                                                                                   cAGAAGCCGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCt                                                                                                                                               <  2:314413/151‑1 (MQ=255) cAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTcca                                                                                                                                               <  2:711661/151‑2 (MQ=255) cAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCt                                                                                                                                               <  1:862555/151‑1 (MQ=255)    aagaCGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGc                                                                                                                                            >  2:861037/1‑151 (MQ=255)     agaCGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCg                                                                                                                                           <  2:339932/151‑1 (MQ=255)         gCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑aa                                                                                                                                      <  2:727865/151‑1 (MQ=255)         gCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑aa                                                                                                                                      <  1:145719/151‑1 (MQ=255)            gCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAg                                                                                                                                     <  2:1032554/149‑1 (MQ=255)            gCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGcc                                                                                                                                   <  2:862330/151‑1 (MQ=255)            gCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGc                                                                                                                                    <  2:347729/150‑1 (MQ=255)             ccccGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCt                                                                                                                                  >  1:1002291/1‑151 (MQ=255)                      gAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTTAATCACCg                                                                                                                         <  1:1237122/151‑1 (MQ=255)                       aGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGc                                                                                                                        <  1:948886/151‑1 (MQ=255)                         cAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGcc                                                                                                                       >  1:391162/1‑150 (MQ=255)                          aaaGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGcc                                                                                                                       >  1:99657/1‑149 (MQ=255)                          aaaGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGcc                                                                                                                       >  1:975442/1‑149 (MQ=255)                            aGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCa                                                                                                                      >  2:853160/1‑148 (MQ=255)                              ccgccAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTa                                                                                                                   >  2:367078/1‑149 (MQ=255)                              ccgccAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTa                                                                                                                   >  1:1166436/1‑149 (MQ=255)                                  cAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAAc                                                                                                             >  2:491268/1‑151 (MQ=255)                                     cccGGCGCGAATCGCGCCAGCCCCCGCGCCCTGCACCAGCGCCGCAGCTTGTGCGTGAAATGCATCAGCCAGCACTTTTCCCACCCGCTGGGTAACGCGCGGTTGATTCGTTCCTGGGGTT‑AAGCCAAAATCACCGCCAGTAAGAAACTc                                                                                                           <  2:983064/150‑1 (MQ=255)                                       cGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCt                                                                                                        >  1:1015209/1‑151 (MQ=255)                                          cgcgAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGcc                                                                                                     >  2:631424/1‑151 (MQ=255)                                             aatccgcgcaggcccccccccccTCCCCCGGCCCCGCATCCTGGGGTTGAAATACATCAGCTAGCCCCGGTCCCACCCGCGGGGAACGGCGCGGTTGATTCGGTCCTGCTGTT‑AAGCCTAAACAACCGCCAGTAAGAAACTCGCTg                                                                                                       <  2:812041/133‑1 (MQ=255)                                              aaTCGCGCCAGCCCCCGCGCCCTGCCCCAGCGCGGCAGCCTGTGCGTAAAATGCAGCAGCCAGCACCTGTTCCACACGTGGGGTAAGGCGCGGTGGAGTCGGTCCGGGGGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCgg                                                                                                   <  2:296455/149‑1 (MQ=255)                                                 cgctccaccccccGCGCCCCCCGCGCGCGCCCCAGCCTGTGCGTGAAATCCTTCAGCCGGCCCCTGTCCCACACGCTGGATACCGCGCGGTTGATCCGCCCCTGGGATA‑AACCATAAACCACCGCCAGTAAGAAACTCGCTGCCGGGGaa                                                                                               <  2:167832/141‑1 (MQ=255)                                                 cgccccgccccccccccccTGCACCAGCCCCGCAGCCTGTGCGTAAAATGCACCAGCCAGCACTTTTTCCACACGTTGTGTAACGCGGGGTCGA‑TCGGGTCGGGCTTTAAAGCCAAAGTCACCGCCAGTAAGAAACTCGCTGCCGGGGaaa                                                                                              <  2:182861/142‑1 (MQ=255)                                                  gcgcCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAAt                                                                                             >  1:1246918/1‑151 (MQ=255)                                                   cgcCGGCCCCCGCGCCCGGCGCCACCGCCGCGCCCTGTGCGGGAAAGGAATCAGCAAGCACTTGTTCCACACGTTGGTTACGGCGCGGTTGATCCGGTCCTGGGGTT‑AAGCCTAAAACACCGCCAGTAAGAAACTCGCTGCCGGGGa                                                                                                <  1:940089/147‑1 (MQ=255)                                                     ccAGCCCCCGCCCCCTGCACCCGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCCCCTGTTCCACACGCGGGGAAACGGGGGGTTGATTCGGTCCGGGCGTT‑AAGCCTAAATCCCCGCCAGTAAGAAACTCGCTGCCGGGGAAAt                                                                                             <  2:1226206/148‑1 (MQ=255)                                                          ccccGCGCCCGGCCCCAGCGCCGCGCCCTGTGCGTGAAATGCATCACCCGCCCCTTGTCCCACACGCGGGGTAACGCGCGGTTGTTTCGGTCCTGGCGTT‑AAGCCAAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACa                                                                                      <  1:620064/150‑1 (MQ=255)                                                               cgcCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCt                                                                                >  1:432220/1‑151 (MQ=255)                                                               cccccGGCACCAGCGCCGCAGCCTGGGCGTGAAATGCATCAGCCACCCCCTTTTCCACACGCTGGGTAACGCGCGGTTGATTGGGTCCGGGCGTA‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGc                                                                                 <  2:1108706/148‑1 (MQ=255)                                                                 cccgcccccggcgcCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTCCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCGGGAGTT‑AACCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTa                                                                               <  1:419573/140‑1 (MQ=255)                                                                 cccTGCCCCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCCCCTGTCCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGc                                                                                 <  1:40309/148‑1 (MQ=255)                                                                 cccTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTAt                                                                              >  2:24093/1‑151 (MQ=255)                                                                         cAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAgc                                                                      >  2:476568/1‑151 (MQ=255)                                                                              ccgcAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCaa                                                                  >  2:700797/1‑150 (MQ=255)                                                                              ccgcAGCCTGTGCGTAAAATGCATCAGCCAGCCCCTGTCCCACCCGCGGGGAAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAgcgc                                                                    <  1:788913/148‑1 (MQ=255)                                                                                gcAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACt                                                               >  1:1229539/1‑151 (MQ=255)                                                                                 cAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTg                                                              >  1:134699/1‑151 (MQ=255)                                                                                 cAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTg                                                              >  2:344121/1‑151 (MQ=255)                                                                                   gCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCt                                                            >  1:387153/1‑151 (MQ=255)                                                                                    ccTGTGCGTAAAATGCATCAGCCAGCCCCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCt                                                            <  1:603193/150‑1 (MQ=255)                                                                                       gtgCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCtttt                                                         <  1:894946/150‑1 (MQ=255)                                                                                        tgCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTtgc                                                       >  1:1221642/1‑151 (MQ=255)                                                                                          cGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTtgctg                                                     >  1:858056/1‑151 (MQ=255)                                                                                             gAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTgcgc                                                  >  1:516540/1‑151 (MQ=255)                                                                                                       agccagcACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATc                                        >  1:1192071/1‑151 (MQ=255)                                                                                                         ccagcCCCTTTCCCACACGCTGGGTACCGCGCGGTTGATTCGGTCCGGGCGTA‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCaat                                            <  2:396689/145‑1 (MQ=255)                                                                                                         ccagcACCTGTCCCACCCGCTGGGTAACGCGCGTTTGATTCGTTCCTGGCGTT‑AAGCTTAAATCCCCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTc                                               <  1:1193683/142‑1 (MQ=255)                                                                                                           agcACCTTTCCCACCCGCTGGGTAACGCGCGGTTGTTTCGTTCCTGGTTTT‑AAGCATAAATCACCGCCAGTAAGAAACTCGCTGCCTGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGt                                      <  2:537089/149‑1 (MQ=255)                                                                                                           acacccTGTTCCACCCGCGGGGTACCGGGCGGTGGATCCGTCCCTGGCTTA‑AGGCCAAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTc                                     <  2:370890/146‑1 (MQ=255)                                                                                                              aCCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTTAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGc                                 >  2:1071056/1‑151 (MQ=255)                                                                                                              aCCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAgg                                  >  2:1234226/1‑150 (MQ=255)                                                                                                              aCCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGc                                 >  2:539188/1‑151 (MQ=255)                                                                                                              aCCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGc                                 >  2:23847/1‑151 (MQ=255)                                                                                                               ccTGTTCCACACGCTGGTTACCGCGCGGTTGATTGGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGTGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCt                                <  1:214371/151‑1 (MQ=255)                                                                                                                        cacGCTGGTTACCGCGCGTTTGATTGGGTCCGGGCGTA‑AAGCATAAACCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCag                          <  1:761245/148‑1 (MQ=255)                                                                                                                        cacGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAAAGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCATTGCagacg                       >  2:671203/1‑149 (MQ=255)                                                                                                                              gggTAACGCGCGGTTGATTCGGTCCTGGGGTA‑AACCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACg                  <  1:1229214/150‑1 (MQ=255)                                                                                                                              gggTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAAc                   <  1:631445/149‑1 (MQ=255)                                                                                                                               ggTAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTTAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTc                >  1:1064373/1‑151 (MQ=255)                                                                                                                                 tAACGCGCGGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCtt              >  1:905547/1‑151 (MQ=255)                                                                                                                                  aaCGCGGGTTTGTTTCGGTCTGGGCGTA‑AAGCCAAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCtt              <  1:595272/150‑1 (MQ=255)                                                                                                                                    cgcgcgGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCAt           >  1:71287/1‑151 (MQ=255)                                                                                                                                        cgGTTGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGt       >  1:346238/1‑151 (MQ=255)                                                                                                                                         gtttGATTCGGTCCGGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGt       <  1:804010/148‑1 (MQ=255)                                                                                                                                           tggATTCGTTCCGGGCTTT‑AAGCATAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGt       <  2:1138132/146‑1 (MQ=255)                                                                                                                                            tGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGcct   >  1:576215/1‑151 (MQ=255)                                                                                                                                            tGATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGcct   >  2:919726/1‑151 (MQ=255)                                                                                                                                             gATTCGGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGcctt  >  2:437354/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                   CAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTT‑AAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTT  >  NC_000913/3505739‑3506029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑