Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,338,117 (A)8→7 intergenic (‑188/+137) adiY ← / ← adiA adi system transcriptional activator/arginine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,338,1100A.98.7% 352.2 / ‑1.7 76intergenic (‑181/+144)adiY/adiAadi system transcriptional activator/arginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base . (38/37);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

GCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGT  >  NC_000913/4337967‑4338254
                                                                                                                                                |                                                                                                                                                
gCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCaaaaaaag                                                                                                                                           <  1:862631/151‑2 (MQ=255)
  tAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCaaaaaaagcg                                                                                                                                         >  2:987422/1‑148 (MQ=255)
   aaagcaaagcGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCaaaaaaagcg                                                                                                                                         >  2:767049/1‑147 (MQ=255)
   aaagcaaagcGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCaaaaaaagc                                                                                                                                          <  1:754641/149‑3 (MQ=255)
    aagcaaagcGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGcgcg                                                                                                                                      <  1:970716/151‑1 (MQ=255)
       caaagcGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCgggg                                                                                                                                   <  1:198313/151‑1 (MQ=255)
        aaagcGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCgggg                                                                                                                                   <  2:96156/150‑1 (MQ=255)
         aagcGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTg                                                                                                                                 <  2:1002059/151‑1 (MQ=255)
         aagcGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTg                                                                                                                                 >  1:240009/1‑151 (MQ=255)
          agcGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTgg                                                                                                                                >  2:1037792/1‑151 (MQ=255)
             gATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTgggg                                                                                                                              <  2:543082/150‑1 (MQ=255)
                      aaaTGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCaaaa                                                                                                                    <  1:159557/151‑1 (MQ=255)
                          ggTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGt                                                                                                                 >  1:230172/1‑150 (MQ=255)
                             tatataATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATg                                                                                                              <  2:553317/150‑1 (MQ=255)
                              atataATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGa                                                                                                             <  1:826913/150‑1 (MQ=255)
                              atataATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGAc                                                                                                            <  2:862606/151‑1 (MQ=255)
                               tataATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGAc                                                                                                            >  1:245018/1‑150 (MQ=255)
                               tataATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGAc                                                                                                            >  1:583530/1‑150 (MQ=255)
                                  aaTTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACAttt                                                                                                        >  1:127762/1‑151 (MQ=255)
                                  aaTTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACAtt                                                                                                         <  2:225799/150‑1 (MQ=255)
                                      gTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTtttgt                                                                                                    >  1:699740/1‑151 (MQ=255)
                                        atgatgAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTtttgttt                                                                                                  <  2:954497/151‑1 (MQ=255)
                                         tgatgaATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTtttgtttg                                                                                                 >  2:581427/1‑151 (MQ=255)
                                         tgatgaATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTtttgttt                                                                                                  <  1:429538/150‑1 (MQ=255)
                                          gatgaATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTtttgtttg                                                                                                 <  2:692202/150‑1 (MQ=255)
                                          gatgaAAATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTtttgt                                                                                                    <  2:527407/147‑1 (MQ=255)
                                           atgaATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTgc                                                                                                >  2:788232/1‑150 (MQ=255)
                                              aaTATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTgcagca                                                                                            <  1:122951/151‑1 (MQ=255)
                                                 ataAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAAtt                                                                                         >  1:933764/1‑151 (MQ=255)
                                                 ataAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAAt                                                                                          <  1:218244/150‑1 (MQ=255)
                                                 ataAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAAt                                                                                          <  1:160716/150‑1 (MQ=255)
                                                     aCATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTa                                                                                     <  1:778313/151‑1 (MQ=255)
                                                      cATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTAt                                                                                    >  2:588757/1‑151 (MQ=255)
                                                       aTGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATg                                                                                   <  2:604751/151‑1 (MQ=255)
                                                           aTAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGaaa                                                                                >  2:318404/1‑150 (MQ=255)
                                                             aaaaaTAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAAc                                                                              <  2:1094093/150‑1 (MQ=255)
                                                             aaaaaTAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACg                                                                             <  1:445297/151‑1 (MQ=255)
                                                               aaaTAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGtt                                                                           >  2:348619/1‑151 (MQ=255)
                                                                 aTAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGc                                                                         >  1:1074001/1‑151 (MQ=255)
                                                                   aaCGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGcc                                                                        >  2:52183/1‑150 (MQ=255)
                                                                     cGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATa                                                                     <  1:229529/151‑1 (MQ=255)
                                                                       aGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATAc                                                                    <  1:1099228/150‑1 (MQ=255)
                                                                             aCTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCaaaa                                                              <  1:330260/150‑1 (MQ=255)
                                                                              cTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAgg                                                            >  1:375853/1‑151 (MQ=255)
                                                                              cTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGGATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAgg                                                            >  1:295736/1‑151 (MQ=255)
                                                                                 aTAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCt                                                         >  1:424065/1‑151 (MQ=255)
                                                                                  tAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCt                                                         >  2:927424/1‑150 (MQ=255)
                                                                                   aGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTa                                                        >  2:1064707/1‑150 (MQ=255)
                                                                                     tACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAg                                                      <  2:466101/150‑1 (MQ=255)
                                                                                      aCAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAg                                                      <  1:957106/150‑1 (MQ=255)
                                                                                      aCAAATTTTAATAGTAAATTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGc                                                    <  1:247916/151‑1 (MQ=255)
                                                                                       cAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGcc                                                   >  2:715698/1‑151 (MQ=255)
                                                                                         aaTTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCa                                                  <  2:784485/151‑1 (MQ=255)
                                                                                          aTTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAgg                                                <  2:1129781/151‑1 (MQ=255)
                                                                                           tttttATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAggc                                               >  2:383234/1‑151 (MQ=255)
                                                                                             tttATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAggcgg                                             >  1:151083/1‑151 (MQ=255)
                                                                                              ttATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAggcggc                                            >  2:247489/1‑151 (MQ=255)
                                                                                              ttATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAggcggc                                            >  2:465962/1‑151 (MQ=255)
                                                                                                 taGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGt                                          >  2:574794/1‑150 (MQ=255)
                                                                                                  aGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTa                                         <  2:226835/150‑1 (MQ=255)
                                                                                                      aTTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGtt                                    <  2:185901/151‑1 (MQ=255)
                                                                                                       tttCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGtt                                    >  1:144630/1‑150 (MQ=255)
                                                                                                            cATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTAtt                                 <  2:132066/148‑1 (MQ=255)
                                                                                                                 aCATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAAt                         >  1:674523/1‑151 (MQ=255)
                                                                                                                  cATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAAtt                        >  2:573497/1‑151 (MQ=255)
                                                                                                                  cATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAAtt                        >  2:901931/1‑151 (MQ=255)
                                                                                                                     atTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTAc                      <  1:3998/150‑1 (MQ=255)
                                                                                                                     atTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTAc                      <  2:280069/150‑1 (MQ=255)
                                                                                                                       tttttttACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGc                    >  1:19526/1‑151 (MQ=255)
                                                                                                                        ttttttACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTCTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGcc                   >  2:416585/1‑151 (MQ=255)
                                                                                                                              aCAGAATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAg              <  2:442193/150‑1 (MQ=255)
                                                                                                                               cAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCg            >  2:779659/1‑151 (MQ=255)
                                                                                                                                aGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCgg           >  1:741318/1‑151 (MQ=255)
                                                                                                                                   ataaataaCAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAGGTATGACATTTTTGATTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAAt        >  1:370813/1‑151 (MQ=255)
                                                                                                                                      aataaCAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAg      >  1:202318/1‑150 (MQ=255)
                                                                                                                                      aataaCAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGt     <  1:959640/151‑1 (MQ=255)
                                                                                                                                       ataaCAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTg    <  2:371553/151‑1 (MQ=255)
                                                                                                                                       ataaCAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTg    <  2:415133/151‑1 (MQ=255)
                                                                                                                                         aaCAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGt  >  1:538598/1‑151 (MQ=255)
                                                                                                                                         aaCAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGt  >  2:1098484/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGT  >  NC_000913/4337967‑4338254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: