Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	218,835	(A)8→7	intergenic (‑60/+52)	proS ← / ← tsaA	prolyl‑tRNA synthetase/tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	218,828	0	A	.	100.0%	362.7 / NA	76	intergenic (‑53/+59)	proS/tsaA	prolyl‑tRNA synthetase/tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (30/46);  total (30/46)

GATCATCCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGT  >  NC_000913/218680‑218973                                                                                                                                                     |                                                                                                                                                  gATCATCCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACaaa                                                                                                                                                 <  2:1054075/151‑1 (MQ=255)    catcCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACaaaaaa                                                                                                                                              <  2:931090/151‑1 (MQ=255)       cccGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACaaaaaaagt                                                                                                                                           >  2:873850/1‑149 (MQ=255)            cgcgcAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATTTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGAttt                                                                                                                                     <  1:717770/151‑1 (MQ=255)            cgcgcAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGTTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGAttt                                                                                                                                     <  2:766697/151‑1 (MQ=255)             gcgcAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGAttt                                                                                                                                     >  2:656076/1‑150 (MQ=255)              cgcAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAg                                                                                                                                   <  2:455470/151‑1 (MQ=255)                cagcaTCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGtt                                                                                                                                 <  1:981174/151‑1 (MQ=255)                cagcaTCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGtt                                                                                                                                 <  1:285659/151‑1 (MQ=255)                 agcaTCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGttt                                                                                                                                >  2:1025633/1‑151 (MQ=255)                 agcaTCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGttt                                                                                                                                <  2:1187944/151‑1 (MQ=255)                      cAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTAcc                                                                                                                             >  2:396396/1‑149 (MQ=255)                      cAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCa                                                                                                                            <  2:167428/150‑1 (MQ=255)                      cAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAg                                                                                                                           <  2:528033/151‑1 (MQ=255)                         cTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTAc                                                                                                                              >  1:311973/1‑145 (MQ=255)                           gATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCa                                                                                                                       >  2:417656/1‑150 (MQ=255)                              ggCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAg                                                                                                                   <  1:1123073/151‑1 (MQ=255)                               gCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGa                                                                                                                  <  1:250701/151‑1 (MQ=255)                                 tGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGaa                                                                                                                 >  1:56859/1‑150 (MQ=255)                                    tCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGAGATTTAGTTTACCAGTGAAAAAGAAATg                                                                                                              >  1:395962/1‑150 (MQ=255)                                           ggCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCaaa                                                                                                         >  2:685180/1‑148 (MQ=255)                                            gCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAagag                                                                                                     <  1:894863/151‑1 (MQ=255)                                             cGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAag                                                                                                       >  1:723363/1‑148 (MQ=255)                                              gTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCCGTTGGAAGGGAGAACAGGCCCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAgaga                                                                                                    >  1:948872/1‑150 (MQ=255)                                              gTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAgaga                                                                                                    >  1:677298/1‑150 (MQ=255)                                              gTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAgaga                                                                                                    <  1:1099301/150‑1 (MQ=255)                                              gTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGaa                                                                                                   <  2:697064/151‑1 (MQ=255)                                                cGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAgg                                                                                                 <  1:1105363/151‑1 (MQ=255)                                                cGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAg                                                                                                  <  1:908259/150‑1 (MQ=255)                                                 ggCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAggg                                                                                                >  2:268466/1‑151 (MQ=255)                                                  gCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGc                                                                                               <  1:253037/151‑1 (MQ=255)                                                    aGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCg                                                                                              >  2:454271/1‑150 (MQ=255)                                                    aGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGt                                                                                             <  1:500532/151‑1 (MQ=255)                                                      gtgtCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGa                                                                                           <  1:744236/151‑1 (MQ=255)                                                        gtCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGaa                                                                                          <  2:1180732/150‑1 (MQ=255)                                                         tctcCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAAtt                                                                                        >  2:360776/1‑151 (MQ=255)                                                            ccTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAAtt                                                                                        <  2:560710/148‑1 (MQ=255)                                                                 agagTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCgg                                                                                <  1:402469/151‑1 (MQ=255)                                                                  gagTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGt                                                                               <  2:671213/151‑1 (MQ=255)                                                                   agTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGtt                                                                              <  2:712076/151‑1 (MQ=255)                                                                        agagCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAgc                                                                         >  2:530425/1‑151 (MQ=255)                                                                         gagCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAgcg                                                                        <  1:709162/151‑1 (MQ=255)                                                                         gagCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAgcg                                                                        >  1:690626/1‑151 (MQ=255)                                                                             aGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCaaa                                                                    <  2:523312/151‑1 (MQ=255)                                                                              ggTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAg                                                                   <  2:374372/151‑1 (MQ=255)                                                                                 aTTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGAc                                                                 <  2:409280/150‑1 (MQ=255)                                                                                      cTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCaa                                                            >  1:1164970/1‑150 (MQ=255)                                                                                          tACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCt                                                       >  2:225251/1‑151 (MQ=255)                                                                                            cGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGc                                                     <  2:361845/151‑1 (MQ=255)                                                                                            cGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGc                                                     <  1:268405/151‑1 (MQ=255)                                                                                             gCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCg                                                    >  2:345430/1‑151 (MQ=255)                                                                                              cATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGt                                                   <  1:1138743/151‑1 (MQ=255)                                                                                               aTGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGt                                                   <  2:436393/150‑1 (MQ=255)                                                                                               aTGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTc                                                  <  2:1140323/151‑1 (MQ=255)                                                                                                   tgttACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTg                                              >  2:725767/1‑151 (MQ=255)                                                                                                    gttACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTg                                              <  1:186388/150‑1 (MQ=255)                                                                                                      tACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGAc                                            <  2:14335/150‑1 (MQ=255)                                                                                                        cGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGAcgcg                                         <  2:26074/151‑1 (MQ=255)                                                                                                            tCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCa                                      <  1:1025392/150‑1 (MQ=255)                                                                                                             ccAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGc                                    <  2:373660/151‑1 (MQ=255)                                                                                                               aGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCg                                   >  2:1008291/1‑150 (MQ=255)                                                                                                                  tGGTAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACg                               >  2:80979/1‑151 (MQ=255)                                                                                                                  tGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACg                               >  2:908924/1‑151 (MQ=255)                                                                                                                        gTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTaaaa                         <  1:1166275/151‑1 (MQ=255)                                                                                                                         tAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAAt                        >  1:406016/1‑151 (MQ=255)                                                                                                                            aaCAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCAt                     <  1:591648/151‑1 (MQ=255)                                                                                                                             aCAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATg                    >  2:171243/1‑151 (MQ=255)                                                                                                                               aGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGt                   >  1:193025/1‑150 (MQ=255)                                                                                                                               aGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTa                  <  2:298137/151‑1 (MQ=255)                                                                                                                                ggCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAg                 <  1:1008058/151‑1 (MQ=255)                                                                                                                                     aaGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGa            <  1:915774/151‑1 (MQ=255)                                                                                                                                      aGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGAc           <  1:345348/151‑1 (MQ=255)                                                                                                                                      aGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGAc           >  1:917848/1‑151 (MQ=255)                                                                                                                                      aGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGAc           <  1:1004075/151‑1 (MQ=255)                                                                                                                                      aGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGAc           <  2:183589/151‑1 (MQ=255)                                                                                                                                        gCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACgg         >  2:711505/1‑151 (MQ=255)                                                                                                                                            gCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCAt      >  2:713514/1‑150 (MQ=255)                                                                                                                                             ccTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAg    <  2:717937/151‑1 (MQ=255)                                                                                                                                               tGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGt  >  1:255084/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GATCATCCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGT  >  NC_000913/218680‑218973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑