Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 346,248 (A)7→8 coding (90/672 nt) yahN ← amino acid exporter for proline, lysine, glutamate, homoserine

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913346,2411.A97.6% 320.8 / 0.2 82coding (97/672 nt)yahNamino acid exporter for proline, lysine, glutamate, homoserine
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base A (38/42);  total (40/42)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.03e-01

CACACTGCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTA  >  NC_000913/346091‑346385
                                                                                                                                                       |                                                                                                                                                 
cacaCTGCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGAtt                                                                                                                                                    <  1:785935/151‑1 (MQ=255)
cacaCTGCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGAtt                                                                                                                                                    >  2:848902/1‑151 (MQ=255)
  cacTGCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTa                                                                                                                                                  >  1:698421/1‑150 (MQ=255)
   acTGCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTaaa                                                                                                                                                <  1:198913/151‑1 (MQ=255)
   acTGCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTaa                                                                                                                                                 >  2:622062/1‑150 (MQ=255)
     tGCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTaaaa                                                                                                                                              >  1:338466/1‑150 (MQ=255)
      gCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTaaaa                                                                                                                                              <  2:1172494/149‑1 (MQ=255)
          aaTTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTaaaaaaaag                                                                                                                                         <  2:753865/150‑3 (MQ=255)
          aaTTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTaaaaaaaag                                                                                                                                         >  2:983198/1‑148 (MQ=255)
           aTTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTaaaaaaaagta                                                                                                                                       <  1:704855/151‑5 (MQ=255)
             tAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAAt                                                                                                                                      >  1:975404/1‑151 (MQ=255)
             tAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTaaaaaaaagtaa                                                                                                                                      <  2:622663/150‑6 (MQ=255)
              aGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATc                                                                                                                                     <  2:927648/151‑1 (MQ=255)
              aGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATc                                                                                                                                     <  1:893894/151‑1 (MQ=255)
                    gCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGaa                                                                                                                                <  2:668333/150‑1 (MQ=255)
                     cAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACa                                                                                                                              <  1:915324/151‑1 (MQ=255)
                      aaGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAg                                                                                                                             <  2:636111/151‑1 (MQ=255)
                        gACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTc                                                                                                                           <  2:997661/151‑1 (MQ=255)
                           caaacaaaCCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCt                                                                                                                         >  2:369324/1‑150 (MQ=255)
                            aaacaaacCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTa                                                                                                                        >  1:791421/1‑150 (MQ=255)
                               caaacCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGt                                                                                                                    >  1:1148533/1‑151 (MQ=255)
                                 aacccgacccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTc                                                                                                                   >  1:983907/1‑150 (MQ=255)
                                  acccaacccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAg                                                                                                                 <  1:549632/151‑1 (MQ=255)
                                  acccaacccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGGTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTc                                                                                                                   >  1:1046123/1‑149 (MQ=255)
                                     caacccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTa                                                                                                              <  1:820345/151‑1 (MQ=255)
                                      aacccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTa                                                                                                              >  2:305780/1‑150 (MQ=255)
                                      aacccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTa                                                                                                              >  1:870756/1‑150 (MQ=255)
                                       acccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTATGGTCAGGTaaa                                                                                                            <  1:755968/151‑1 (MQ=255)
                                       acccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTaa                                                                                                             >  2:897724/1‑150 (MQ=255)
                                        cccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTaa                                                                                                             >  2:795404/1‑149 (MQ=255)
                                        cccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAAc                                                                                                           <  2:876231/151‑1 (MQ=255)
                                        cccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACg                                                                                                          >  1:920715/1‑151 (MQ=255)
                                          cGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACgg                                                                                                         >  2:570538/1‑151 (MQ=255)
                                            gAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGc                                                                                                        >  2:305697/1‑150 (MQ=255)
                                             aaTAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCAt                                                                                                      >  2:448794/1‑151 (MQ=255)
                                              aTAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATg                                                                                                     >  1:666377/1‑151 (MQ=255)
                                                aaaaTGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGc                                                                                                    >  1:1066040/1‑150 (MQ=255)
                                                   aTGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAg                                                                                                <  2:616994/151‑1 (MQ=255)
                                                         cGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCa                                                                                          <  2:448929/151‑1 (MQ=255)
                                                            ccAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCAt                                                                                         >  1:916947/1‑149 (MQ=255)
                                                              aGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTg                                                                                     >  2:694745/1‑151 (MQ=255)
                                                                 gcCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGAtt                                                                                  >  2:520887/1‑151 (MQ=255)
                                                                  ccACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGAttt                                                                                 <  1:848707/151‑1 (MQ=255)
                                                                     cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCAt                                                                              <  1:906329/151‑1 (MQ=255)
                                                                     cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCAt                                                                              <  2:73567/151‑1 (MQ=255)
                                                                         cAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCat                                                                          >  1:285769/1‑151 (MQ=255)
                                                                         cAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCat                                                                          <  1:1120743/151‑1 (MQ=255)
                                                                          aGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCat                                                                          >  1:121003/1‑150 (MQ=255)
                                                                            cccGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCataaa                                                                       <  1:345579/151‑1 (MQ=255)
                                                                             ccGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCataaat                                                                      <  1:515384/151‑1 (MQ=255)
                                                                                  cAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTg                                                                 <  1:130044/151‑1 (MQ=255)
                                                                                    gCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGa                                                                <  1:535775/150‑1 (MQ=255)
                                                                                     cACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaa                                                               <  2:561505/150‑1 (MQ=255)
                                                                                      aCCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaact                                                             >  2:468351/1‑151 (MQ=255)
                                                                                      aCCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACAACACAGAAATTGGCTCCCGGATTAAAAAAAAGAAATCACGAACAGTCTTACGGTCAGGTAAACGGCATGCAAAGGAGCCACAGTGATTTCATCCATAAATAAGTGaact                                                             >  1:621394/1‑151 (MQ=255)
                                                                                        cccTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaactaa                                                           <  1:97747/151‑1 (MQ=255)
                                                                                            gcgcGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTg                                                        <  1:420389/150‑1 (MQ=255)
                                                                                              gcgTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGcat                                                     >  1:652024/1‑151 (MQ=255)
                                                                                               cgTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGcatc                                                    <  1:707161/151‑1 (MQ=255)
                                                                                                 tCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGcatcat                                                  >  1:1066873/1‑151 (MQ=255)
                                                                                                  cGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCatat                                                >  2:104432/1‑151 (MQ=255)
                                                                                                    aCCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATAtt                                               >  2:356491/1‑151 (MQ=255)
                                                                                                     ccGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATAttt                                              <  2:1013508/151‑1 (MQ=255)
                                                                                                         aaGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTAc                                          >  2:50552/1‑151 (MQ=255)
                                                                                                             cAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCaaa                                      <  1:840157/151‑1 (MQ=255)
                                                                                                             cAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCaaa                                      <  2:698589/151‑1 (MQ=255)
                                                                                                             cAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCaa                                       >  1:1047690/1‑150 (MQ=255)
                                                                                                              aGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTCCATCATATTTCTACCa                                        <  1:688488/148‑1 (MQ=255)
                                                                                                                  tGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAaata                                  >  2:1064966/1‑150 (MQ=255)
                                                                                                                   ggTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAaataat                                <  2:598299/151‑1 (MQ=255)
                                                                                                                    gTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                               <  1:871447/151‑1 (MQ=255)
                                                                                                                    gTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                               <  2:917159/151‑1 (MQ=255)
                                                                                                                     tttGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCg                              >  2:936805/1‑151 (MQ=255)
                                                                                                                       tGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCgg                             <  2:927030/150‑1 (MQ=255)
                                                                                                                       tGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCgg                             <  2:783194/150‑1 (MQ=255)
                                                                                                                         tactacCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGt                           <  1:526568/150‑1 (MQ=255)
                                                                                                                          actacCACAAAGAGATTGGCTCCCAGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTg                          <  1:248018/150‑1 (MQ=255)
                                                                                                                             acCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCg                        >  2:1140601/1‑149 (MQ=255)
                                                                                                                             acCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCg                        >  1:619091/1‑149 (MQ=255)
                                                                                                                                acaAAGAGATTGGCTCCCGGATTAAATAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgag                      >  2:783062/1‑148 (MQ=255)
                                                                                                                                 caAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagagag                  <  2:98453/151‑1 (MQ=255)
                                                                                                                                 caAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagag                    >  1:1044148/1‑149 (MQ=255)
                                                                                                                                   aaGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagag                    >  2:906342/1‑147 (MQ=255)
                                                                                                                                    agagATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagag                    >  2:66198/1‑146 (MQ=255)
                                                                                                                                      agaTTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATcac             <  1:512249/151‑1 (MQ=255)
                                                                                                                                       gaTTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATcac             >  2:269675/1‑150 (MQ=255)
                                                                                                                                          tGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACaaa          <  2:670971/150‑1 (MQ=255)
                                                                                                                                            gCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAgtg       <  2:371063/151‑1 (MQ=255)
                                                                                                                                            gCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAgtg       >  2:717859/1‑151 (MQ=255)
                                                                                                                                               cccGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAgtgt      <  1:621917/149‑1 (MQ=255)
                                                                                                                                               cccGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCt    >  1:465486/1‑151 (MQ=255)
                                                                                                                                                 cGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTa  <  2:1070852/151‑1 (MQ=255)
                                                                                                                                                 cGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTa  <  1:128672/151‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                 
CACACTGCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTA  >  NC_000913/346091‑346385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: