Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,097,0301.G78.3% 85.1 / 24.7 46intergenic (+201/+535)ycdU/serXputative inner membrane protein/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base . (9/1);  new base G (11/25);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.12e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGC  >  NC_000913/1096884‑1097171
                                                                                                                                                       |                                                                                                                                                   
aTTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTgg‑‑gggg                                                                                                                                                >  2:650776/1‑151 (MQ=255)
aTTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑gggg                                                                                                                                                <  2:1160061/151‑1 (MQ=255)
 ttttGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑ggggg                                                                                                                                               >  1:380418/1‑151 (MQ=255)
 ttttGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑ggggg                                                                                                                                               >  2:573875/1‑151 (MQ=255)
    tGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑ggggggg                                                                                                                                             <  1:95514/150‑1 (MQ=255)
     gATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑ggggggggg                                                                                                                                           >  1:643566/1‑149 (MQ=255)
     gATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑gggggggg                                                                                                                                            >  2:429381/1‑149 (MQ=255)
     gATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑ggggggggat                                                                                                                                          <  2:179476/151‑4 (MQ=255)
      aTTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGAGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGC‑‑gggggggggg                                                                                                                                          >  1:1149/1‑148 (MQ=255)
                 aGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgcttc                                                                                                                               <  1:814476/151‑1 (MQ=255)
                            aGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGGG‑‑GGGGGGGGTTGACTCGCTTCGCTCGccc                                                                                                                       >  1:191529/1‑148 (MQ=255)
                             gTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGACTCCCTTCGCTCcccc                                                                                                                       >  2:244973/1‑147 (MQ=255)
                             gTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCg                                                                                                                   <  2:736886/151‑1 (MQ=255)
                             gTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCg                                                                                                                   <  1:964650/151‑1 (MQ=255)
                                 aTGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGGGTGACTCGCCTCCCTCGCCCCGCggg                                                                                                                 >  1:507525/1‑149 (MQ=18)
                                   gtgCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAg                                                                                                              <  2:961413/150‑1 (MQ=255)
                                    tgCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGAGTGAATCGCTTCGCCCGCCCCGCGGGGAGcc                                                                                                            >  2:514328/1‑151 (MQ=18)
                                    tgCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGGATTGACTCGCTTCTCCCGCCCTCCGGGGAGcc                                                                                                            >  2:964619/1‑151 (MQ=18)
                                       aCGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGGA‑‑GGGGGGGGTTTAGCCGCTTCGCCCCCCCTGCGGGGCGccc                                                                                                           >  1:314094/1‑150 (MQ=18)
                                        cGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGAATCGCCTCGCACGCCCTGCgggaccccc                                                                                                           >  2:605508/1‑142 (MQ=18)
                                          aTTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCa                                                                                                      <  2:661561/151‑1 (MQ=255)
                                            ttCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGGGTGACTCGCCTCCCTCCCCCCGCGGGGGGccccccc                                                                                                       >  1:1006525/1‑144 (MQ=17)
                                              ccccTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGACTCGCTTCGCTCGCCCTGCGGGCAGccc                                                                                                           >  2:1156962/1‑142 (MQ=255)
                                                  tcccccTAACAGACGATGGCGGGGTCACCCGTTGTGTTCTATATACGAAAAAAAAGCCCGTACTTTCGTTCTATCTCTTCTGTAACGACCACCGGGA‑‑GGGCCGATTGACGCGATTCCCTCGCCCTCCCGGCACCCCCCTTACttgatt                                                                                               <  1:860393/145‑6 (MQ=9)
                                                    gCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTTACTCCCCTCGCTCGCCCCGCGGGCAGCCCCCTCCCCGCGTTCAc                                                                                            >  2:609736/1‑151 (MQ=17)
                                                     cccTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCAc                                                                                            <  2:296741/150‑1 (MQ=255)
                                                          aCAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTg                                                                                      <  2:616162/151‑1 (MQ=255)
                                                               cGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCaa                                                                                 <  1:37421/151‑1 (MQ=255)
                                                                    gCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTgg                                                                            <  2:486502/151‑1 (MQ=255)
                                                                     cGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTg                                                                         <  1:559331/151‑1 (MQ=255)
                                                                      gggggCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTg                                                                         <  2:180427/151‑1 (MQ=21)
                                                                                      tgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑GGGGGGGGTTGACTCGCTTCTCTCCCCCTTCGGGCAGCCCGCCCCCCGTGTTCACGGTCTGTCCCACCGGCTGTCGCCCGgtgttggacc                                                      >  1:385474/1‑145 (MQ=1)
                                                                                         aaTATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAcccc                                                    <  1:868774/150‑1 (MQ=14)
                                                                                             taCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAA‑‑GGGGGGGATTGAATCGCCTTCCCCCCCCCGCGGGGAGCCCGCCCCCTGCGTTTACGGGCTTTCCAACCTGCTGTCGCCCCTTTTCGAcaccc                                                   >  2:752579/1‑147 (MQ=2)
                                                                                                gAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCggg                                            <  1:503017/151‑1 (MQ=14)
                                                                                                  aaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCgggg                                           <  1:852492/151‑1 (MQ=14)
                                                                                                   aaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGc                                          <  1:781183/151‑1 (MQ=14)
                                                                                                       aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTtct                                      <  1:1026320/151‑1 (MQ=14)
                                                                                                        aaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTTACCCGCTTCCCTCGCCCTCCGGGCCGCCCCCTCACTGCGTTTCCGGTCTGTCCCACCGGCTGTCGCCAGTTGTCGACCCCCCGTCGGGGGtt                                        >  2:195174/1‑148 (MQ=11)
                                                                                                         aGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑GGGGGGGGTTTACTCGCCTCTCTCCCCCCCCGGGCAGCCCGCTCACTGCGGTCACGGTCTCTCCCACCCGCCGTCGCCCGTTGTcccaccccccgcggg                                             >  1:316744/1‑128 (MQ=0)
                                                                                                               tACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGA‑‑GGGGGGGATTTAGTCTGCTCTCCCGCTCTTCCGGTCGCCCGCTCCCTTCGGTTACGGTCTGTTCCACCGGCGGGCCCCCCCTTTTGACCCCCCGTCGGGGCTTCTCCTcccccc                             >  1:69891/1‑151 (MQ=2)
                                                                                                                 cTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGG‑‑GGGGGGGGTTGACTCCCCTCCGCCCCCCCTGGGGGAGCCCCCCCCCCCCGTTTTCCGGCTGTCCAACATGGCGTCCCCACGTGTTCGACCCCCGGCGGGGGCTTTCATccccccc                            >  2:1105735/1‑150 (MQ=2)
                                                                                                                     cGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGgtg                         <  2:175014/150‑1 (MQ=14)
                                                                                                                     cGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGAGTGACTCGCTTCCCTCGCCCCGCGGGGCCCCCGCCCACCGCGTTCTCCGGCTGTTCAACTGGCTGTCCCCCGGTGTCGACCCCCCGCCGGGGCTTTCCCTccccccc                            >  1:213752/1‑146 (MQ=2)
                                                                                                                      gTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑GGGGGGGGTTTACTCCCCCCCCTCGCCCTCCTGGCAACCCGCTCCCTGGGTTTACGGGCCGTCCCACTGGGCGTTGGCAGCTTTCGCACCCCCGGCGGGGGCTCTCCTcccccccggg                         >  1:147692/1‑144 (MQ=0)
                                                                                                                           aGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCaa                  <  1:244920/151‑1 (MQ=14)
                                                                                                                           aGCTCTTCTTTAAATATGGCGGTGA‑‑GGGGGGGGTTGACTCGCTTCGCTCCCCCCGCGGGGCGCCCGCCCCCCGCGGTTACGGGCTGTTCCACCGGCTGGCGCCCGGTGTTGGACCCCCGGCGGGGGGTCTCCTcccccccggg                         >  1:1177162/1‑139 (MQ=0)
                                                                                                                            gCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAAt                 <  2:16445/151‑1 (MQ=14)
                                                                                                                                 tcttTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATacac             <  1:118941/150‑1 (MQ=18)
                                                                                                                                  cttTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATac               <  2:126532/147‑1 (MQ=17)
                                                                                                                                   tttAAATATGGCGGTGAGGGGGGGGGTTGACTCGCTTCGCCCGCCCTGCGGGCAGCCCGCCCACTGCGTTCACGGGCTGTCCAACTGGCTGTCGCCCGTTGTCGACCCCCCGGCGGGGCTTCTCCTCCCCCCCGGGGGTGGAAAaca              >  2:987002/1‑147 (MQ=11)
                                                                                                                                    ttAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACg            <  2:160346/148‑1 (MQ=18)
                                                                                                                                      aaaTATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGaaaaa       <  2:832558/151‑1 (MQ=18)
                                                                                                                                          atGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGaaaaaaaa  <  1:479084/151‑2 (MQ=18)
                                                                                                                                            ggCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGc  <  2:937232/150‑1 (MQ=18)
                                                                                                                                                       |                                                                                                                                                   
ATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGC  >  NC_000913/1096884‑1097171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: