Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	42.9%	65.6 / 44.2	63	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (4/32);  new base T (27/0);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 6.43e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206287‑3206583                                                                                                                                                      |                                                                                                                                                       gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCa                                                                                                                                                      <  1:435662/151‑1 (MQ=255)   gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAg                                                                                                                                                     <  2:473475/150‑1 (MQ=255)   gtATTTCGCAAAACATTGCCACTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGa                                                                                                                                                    <  2:748284/151‑1 (MQ=255)       ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACaa                                                                                                                                                 <  2:1071895/150‑1 (MQ=255)                      cATTCCCTGCTCTGGCGCAATATTCGTTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGA‑ATTTTTTCCAAcaaaaacaaaaa                                                                                                                                        >  2:1106912/1‑144 (MQ=255)                      cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTCAGACAAATACAAAAACTGGAg                                                                                                                                  >  2:825266/1‑150 (MQ=255)                       aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGtt                                                                                                                                <  2:456758/151‑1 (MQ=255)                        ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTCCAACAAAAACAAAAAATgg                                                                                                                                    >  2:105024/1‑146 (MQ=255)                         tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTCAGACAaaaaaaaaaaaatgaggt                                                                                                                                >  1:721323/1‑141 (MQ=255)                         tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTCAAACAAAAACAAAAAAAGGAggt                                                                                                                                >  1:1019631/1‑147 (MQ=255)                              gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTCCGACAAAAAAAAAAAATGGAGTTggc                                                                                                                             >  2:519685/1‑145 (MQ=255)                                   ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑g                                                                                                                    <  2:842095/150‑1 (MQ=255)                                      gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGaa                                                                                                                <  2:58497/151‑1 (MQ=255)                                       cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGaaa                                                                                                               <  2:439417/151‑1 (MQ=255)                                       cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGaaa                                                                                                               <  2:583649/151‑1 (MQ=255)                                          tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTTAGAAAAAAACAAAAAATGGAGTTGGCatggggggagaaa                                                                                                                >  1:297654/1‑138 (MQ=255)                                          tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑AATTTTTCAAAAAAAAAAAAAAAAAGGAGGTGGCCTGATGA‑GGGAAAg                                                                                                              >  1:97141/1‑149 (MQ=255)                                           attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGtaa                                                                                                           <  2:178908/151‑1 (MQ=255)                                             tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGA‑GCGAAAGtaa                                                                                                           >  2:988710/1‑149 (MQ=255)                                                 tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGc                                                                                                      <  1:1123146/150‑1 (MQ=255)                                                  cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTTAGACAAAAAAAAAAAAATGAGTTTCCATGATGAGGGGAAAGtataaaaa                                                                                                      >  1:483585/1‑144 (MQ=255)                                                        ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGA‑GGGAAAGAAATAAGCAACa                                                                                                  >  2:35691/1‑147 (MQ=255)                                                          cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTCCAACAAAAAAAAAAACTGGAGTTGCCCTGGTGA‑GGGGAAGaaaaaaaaaaaaagcagg                                                                                            >  2:620584/1‑139 (MQ=255)                                                             gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTCAGACAAAAACAAAAAATTGAGTTGGCATGATGA‑GCGGAAGTAATAAACAACAAGCAggg                                                                                           >  2:1025469/1‑147 (MQ=255)                                                               tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAAt                                                                                        <  1:949285/150‑1 (MQ=255)                                                                   tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTCCGACAAAAAAAAAAAATGGAGGTGCCATGATGA‑GGGGAAATAATAAACAACAAGCAGGGAATaa                                                                                      >  2:500338/1‑148 (MQ=255)                                                                     aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGa                                                                                 <  2:840680/151‑1 (MQ=255)                                                                          cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTCCGACAAAAAAAAAAAAAGGAGTTGCCATGATGA‑GCGGAAGGAAAAAACAACAAGCAGTGAAAAAATTGACa                                                                               >  2:140835/1‑148 (MQ=255)                                                                           tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTTAAACAAAAAAAAAAAATTGAGGTGCCCTGGTGG‑GGGAAAGTAAAAAAAAAAAGGCAGTGAAAAAGGTGAAag                                                                              >  1:936370/1‑148 (MQ=255)                                                                            gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                           <  1:207175/150‑1 (MQ=255)                                                                              tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAtt                                                                         <  2:1143413/150‑1 (MQ=255)                                                                               aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTg                                                                        <  1:878324/150‑1 (MQ=255)                                                                               aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGt                                                                       <  2:397914/151‑1 (MQ=255)                                                                                   aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTCAAACAAAAACAAAAAATGGAGTTGCCATGGTGA‑GCGAAAGTAATAAACAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                   >  2:615385/1‑151 (MQ=255)                                                                                     atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTaa                                                                 <  1:767945/151‑1 (MQ=255)                                                                                      tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑AATTTTTCAAACAAAAAAAAAAAATGGAGTTTGCATGATGA‑GCGAAAGTAATAAAAAAAAAGCAGAGAATAAAGTGAAAGAGATTGTCGCTaa                                                                 >  1:947015/1‑150 (MQ=255)                                                                                            cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑AATTTTTCAGACAAAAAAAAAAAATGGAGTTGCCATGATGA‑GGGGAAATAATAAACAAAAAGCAGTGAAAAAATTTACAGAGATTTTTGCTAACTTTTcc                                                          >  1:863500/1‑151 (MQ=255)                                                                                             ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCg                                                         <  1:1138558/151‑1 (MQ=255)                                                                                                ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGcc                                                       <  2:356060/150‑1 (MQ=255)                                                                                                          ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑AATTTTTCAAACAAAAAAAAAAAATGGAGTTTGCATTGTGA‑GGGAAAAAAATAAAAAAAAAGCAGAGAATAAATTTTAAGAGAATGTCGCTAACTTTTCCCCCCCG‑ATTTTTa                                            >  1:874043/1‑150 (MQ=255)                                                                                                            aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTCAAACAAAAAAAAAAACTTTGGGTGGCATGATGA‑GAGGAAAAAATAAACAACAGGCAGGGAATTAATTGACAGAGAGTTTCGAGAAATTTTCCGCCATGAATTTTTAcc                                          >  1:389054/1‑151 (MQ=255)                                                                                                             aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTCAAACAAAAACAAAAAATGGAGTTGGCATGATGA‑GGGGAAGGAATAAAAAAAAAGCAGTGAAAAAATTGACAGAGAATGTCGCTAACTTTACCGCCATG‑Attttttacc                                         >  1:1119785/1‑144 (MQ=255)                                                                                                              aaCCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGa                                       <  2:1107892/151‑1 (MQ=255)                                                                                                                ccACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTCAGACCAAAAAAAAAAATGGAGGTGTCATGATGG‑GGGAAAGTAAAAAAAAAAAAGCCGTGAAAAAATTGAAAGAGAATTTTGCCAACATTTCCGCCATG‑ATTTTTAccc                                         >  1:480343/1‑147 (MQ=255)                                                                                                                 cacaTTTTTAGTGGTAGTTTGTGGCGGGGAGGTTTTTCCA‑ACAAAGAAAAAAAAAGGAGTTTTCGTGGTGA‑GGGAGAGTAAAAAAAAACAGGAAGAGAAAAAGTTGACAAAGATTGTCGCCACATTTTCCGcaccggttt                                                >  1:792968/1‑132 (MQ=255)                                                                                                                     tttttAGTGGTAGTTTGTGGCGGTGG‑ATTTTTTCCGACAAAAAAAAAAAATGGAGTTGCCATGATGA‑GCGGAAGAAATAAACAACAAGGAGGGAATAAATTTAAAGAGATTTTCGCTAAATTTATCCCCCTG‑ATTTTTACCCCAAAGcc                                  >  2:543258/1‑149 (MQ=255)                                                                                                                      ttttAGTGGTATTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACGGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCtg                                <  2:739215/150‑1 (MQ=255)                                                                                                                      ttttAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTCCGACAAAAACAAAAAAAGGAGTTGGCATGATGA‑GCGGAAGGAAAAAAGAAAAAGGAGTGAAAAAGATTAAAGAGGTTGTCGCCAACTTTTCCCCCCTG‑ATTTCTACCCCAATGcccg                                >  2:908305/1‑148 (MQ=255)                                                                                                                      ttttAGTGGTAGTTTGTGGCGGGGA‑ATTTTTTCAGACAAAAACAAAAAATTGAGTTGTCATGATGA‑GGGGAAGAAATAAACAAAAAGCAGTGAAAAAGGTGACAAAGATTGTCGCCAAATTTACCGCCCTG‑ATTTCTACCCCAATg                                    >  1:853634/1‑146 (MQ=255)                                                                                                                         tAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCtgatg                             <  2:721487/150‑1 (MQ=255)                                                                                                                             ggTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAACCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATG‑ATTTCTACCCGAATGCCtgatg                             <  1:205372/146‑1 (MQ=255)                                                                                                                              gTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACgtgg                       <  2:113155/151‑1 (MQ=255)                                                                                                                                aGTTTGTGGCGGTGG‑ATTTTTTTCGACAAATACAAAAAATGGGGTTGCCATGATGG‑GCGAAAGGAATAAAAAAAAGGCCGGGAATAAGTTGACAGAGATTGTCGCCTACTTTACCGCCCAG‑ATTTTTTCCCGAAAGCCCGATGAAgtgggg                     >  1:389586/1‑149 (MQ=255)                                                                                                                                  tttGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGA‑GCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACgtggt                      <  2:239496/148‑1 (MQ=255)                                                                                                                                       ggCGGTAA‑ATTTTTCAAGAAAAATACAAAAATTGGAGTTGCCTTGAGGA‑GCGAAATAAATAAGCAACGGGCATTCAATAAGTTGCCAGGGATTGTCGTTACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGAt                  <  1:596057/147‑1 (MQ=255)                                                                                                                                        gCGGTGA‑ATTTTTCCAGAAAAATACAAAAACGGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt             <  2:1019870/151‑1 (MQ=255)                                                                                                                                            tGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa          >  2:1151431/1‑150 (MQ=255)                                                                                                                                                aTTTTTCCAGACAAATACAAAAACTGGAGTGGCCATGAAGA‑GGGAAAGTAATAAGCAACAGGCAGTGAAGAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAg      <  2:562693/151‑1 (MQ=255)                                                                                                                                                 tttttCCAGACAAATACAAAAACTGGATTTGCCATGATGA‑GCAAAAGTAATAACCAACAGGCAGTAAATAAGTTAACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAg      <  1:590132/150‑1 (MQ=255)                                                                                                                                                  ttttCCATAAAAATAAAAAACCGGGAGTTGCCATGATGA‑GCGAAAGAAATAACCAACAGGCAGTGAATAATTTAACAGAGTTTTTCTATATCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc     <  1:1151171/150‑1 (MQ=255)                                                                                                                                                   tttCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt    >  2:906287/1‑150 (MQ=255)                                                                                                                                                   tttCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt    >  1:452863/1‑150 (MQ=255)                                                                                                                                                   tttCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt    <  2:1176937/150‑1 (MQ=255)                                                                                                                                                      ccAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  <  1:35683/149‑1 (MQ=255)                                                                                                                                                      ccAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAAAGAGATTGTCCCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  <  2:966425/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                       GTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206287‑3206583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑