Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,261,0550AC46.8% 92.5 / 13.3 62V455G (GTG→GGG) tdcEpyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base A (29/4);  new base C (0/29);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.02e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATT  >  NC_000913/3260909‑3261203
                                                                                                                                                   |                                                                                                                                                    
aGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCAcccc                                                                                                                                                  <  2:610569/151‑1 (MQ=255)
   gAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCccgcc                                                                                                                                               <  1:964057/151‑1 (MQ=255)
      taatatTCAGCGCTCTGTTTTACTGCCCCGCCAGCCAGTCCATGAAGTGATCGAGGCTTCCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTAAATCTTCAGCTTCCCTCCCCCCCCGCCGt                                                                                                                                             <  1:774879/148‑1 (MQ=255)
       gATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGtt                                                                                                                                            >  2:1074154/1‑150 (MQ=255)
          atTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATAAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAAtt                                                                                                                                        >  1:571619/1‑151 (MQ=255)
             cAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCg                                                                                                                                     >  1:1004728/1‑151 (MQ=255)
                 gcgcTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGt                                                                                                                                    >  2:878644/1‑148 (MQ=255)
                      gATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGagcagc                                                                                                                            >  1:332535/1‑151 (MQ=255)
                         ttcctccccccccagccagTCCATGAAGTGATCGGGGCTGTCCATCTCTTTGTCGTAACCCAGCCCGTCGCCCATCGGCGGTGCTTTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGt                                                                                                                          <  1:1076418/139‑1 (MQ=255)
                           aCTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGtttt                                                                                                                       >  2:702926/1‑151 (MQ=255)
                              gCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGc                                                                                                                    >  2:184962/1‑151 (MQ=255)
                                ccccccagccagCCCATGAAGTGATCGAGGCTGTCCATCTCTTTGTCGTAATCCAGCACGTCGCCCACCAGCGGTGTTTTTTTCGCCCCGACCTGATTCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCa                                                                                                                  <  1:645525/147‑1 (MQ=255)
                                  cGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAgg                                                                                                                >  1:263592/1‑151 (MQ=255)
                                   gccagccaGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTAGCCAGGt                                                                                                               >  2:981875/1‑151 (MQ=255)
                                    ccagccagTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAACCCAGCCCGTCGTCCATCAGCGGTGCTGTTTTCGCCCCGCCCTAAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGt                                                                                                               <  2:924093/150‑1 (MQ=39)
                                      agccagTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGtt                                                                                                              >  1:121229/1‑149 (MQ=255)
                                        ccagTCCATGAAGTGATCGGGGCTGTCCATCCCTTTGTCGTAATCCAGCACGTCGTCCACCAGCGGTGCTTTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgcg                                                                                                          <  2:987909/151‑1 (MQ=255)
                                        ccagTCCATGAAGTGATCGGGGCTGTCCATCACTTTGTCGTACCCCAGCCCGTCGTCCATCAGCGGTGCTTTTTCCGGCCCGCCCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTa                                                                                                             <  1:1083018/148‑1 (MQ=255)
                                        ccagTCCATGAAGTGATCGAGGCTTTCAATCACTTTGTCGTAACCCAGCACGTCGTCCACCAGCGGTGTTTTTTCCGGCCCGACCTGAATCTTCACCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAg                                                                                                            <  1:257523/149‑1 (MQ=255)
                                         cagTCCATGAAGTGATCGAGGCTGTCCATCTTTTTGTCATAATCCAGCCCGTCTCCCATCAGCGGTGCTGTTTTCGCCCCAACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgcgc                                                                                                         <  2:1042978/151‑1 (MQ=255)
                                         cagTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgcgc                                                                                                         >  1:803957/1‑151 (MQ=255)
                                          agTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgcgc                                                                                                         >  2:408648/1‑150 (MQ=255)
                                             ccATGAAGTGATCGAGGCTGTCCATCCCTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTg                                                                                                      <  2:252309/150‑1 (MQ=255)
                                                    gTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaa                                                                                                >  2:440182/1‑149 (MQ=255)
                                                     tGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaa                                                                                                >  1:179445/1‑148 (MQ=255)
                                                      gATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGa                                                                                             >  1:589836/1‑150 (MQ=255)
                                                       aTCGAGGCTGTCCATCTCTTTGTCGTAACCCAGCCCGTCGTCCATCAGCGGTGCTGTTTTCGCCCCGCCCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGaa                                                                                            <  2:392285/150‑1 (MQ=255)
                                                        tCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACt                                                                                          >  2:384567/1‑151 (MQ=255)
                                                        tCGAGGCTGTCCATCACTTAGGCGAAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGACCACCCCGCCGTTAACTGCGTTGTGCAGCGTTTTCGACAGGATAGCGCTTGCACCAAAGAACt                                                                                          >  1:850754/1‑151 (MQ=255)
                                                          gAGGCTGTCCACCCTTTTGTCGTAATCCAGCACGTCGTCCATCGGCGGTGCTGTTTTCGGCCCGCCCTAAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGaa                                                                                            <  2:314315/147‑1 (MQ=255)
                                                               tGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTg                                                                                   >  1:1011496/1‑151 (MQ=255)
                                                                atccacaccTTTGTCGCAACCCAGCCCCTCGCCCATCGGGGGTGTTTTTTGCGCCCCGACGGGATTCTTCGCCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAttt                                                                                    <  1:663948/141‑1 (MQ=255)
                                                                  ccATCTTTTTGTCGAAACCCAGCACGTCGTCCACCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGc                                                                                  <  1:232960/149‑1 (MQ=255)
                                                                  ccATCACTTTGTCGTAATCCAGCCCGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTg                                                                                   <  2:723209/148‑1 (MQ=255)
                                                                  ccATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCtt                                                                                >  2:513653/1‑151 (MQ=255)
                                                                   cATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCa                                                                                                 >  1:175996/1‑133 (MQ=255)
                                                                       aCTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCa                                                                            >  2:175601/1‑150 (MQ=255)
                                                                            gTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCAcc                                                                      >  1:190334/1‑151 (MQ=255)
                                                                             tCGTAATCCAGCCCGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCa                                                                        <  2:599055/148‑1 (MQ=255)
                                                                               gaaacccAGCCCGTCGTCCACCGGCGGTGCTGTTTTCGGCCCGACCTAAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCAtt                                                                   <  2:1052451/146‑1 (MQ=255)
                                                                               gTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCAtt                                                                   >  1:400737/1‑151 (MQ=255)
                                                                                     cAGCACGTCGTCCATAAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTg                                                             >  1:452131/1‑151 (MQ=255)
                                                                                      aGCACGTCGTCCATCAGCGGTGCTGCTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGCTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTg                                                             >  2:438737/1‑150 (MQ=255)
                                                                                          cgtcgtCCCTCAGCGGTGCTTTTTCCGCCCCGCCCTGAATCTTCACCTCCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGAc                                                           <  1:1045288/148‑1 (MQ=255)
                                                                                           gtcgccCGTCAGGGGTGGTTTTTTCGGCCCGACCGGAATCTTCGGCTTCTCCCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcag                                                       <  2:278940/151‑1 (MQ=255)
                                                                                             cgtcCATCAGCGGGCTTTTTGCCGGCCCGTCCTAAATCCTCAGCTTCCCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagca                                                     <  1:1009088/151‑1 (MQ=255)
                                                                                                     ggtgtgccttttttCGGCCCGTCAAAAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCGCCATTGGGCTGACGCAGCAGGCAATcg                                             <  2:750045/141‑1 (MQ=255)
                                                                                                        ggTGCGGTTTTCGGCCCGACCTGAATCTTCGGCTTCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATcgcg                                           <  1:532890/150‑1 (MQ=255)
                                                                                                           gCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAt                                       >  2:527344/1‑151 (MQ=255)
                                                                                                              gTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcg                                     <  2:125357/150‑1 (MQ=255)
                                                                                                               ttttCGGCCCGCCCTAATTCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtc                                      <  2:1077425/148‑1 (MQ=255)
                                                                                                               ttttCGGCCCGACCTGATTCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcg                                     <  1:1028798/149‑1 (MQ=255)
                                                                                                                tttCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcgtc                                   <  1:548196/150‑1 (MQ=255)
                                                                                                                   tCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCt                                >  2:256265/1‑151 (MQ=255)
                                                                                                                       ccccACTTGAATTTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTg                            <  2:631141/151‑1 (MQ=255)
                                                                                                                                aaTCTCCACCTTCCCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAg                     <  1:702755/149‑1 (MQ=255)
                                                                                                                                    ttcctctcctcctcCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTAc                  <  2:332614/140‑1 (MQ=255)
                                                                                                                                      cggCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATc             <  1:1168796/149‑1 (MQ=255)
                                                                                                                                      cAGCTTCCCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACg                 <  2:582897/147‑1 (MQ=255)
                                                                                                                                            ctcccccACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAg           <  1:440073/142‑1 (MQ=255)
                                                                                                                                                tCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCAt   >  2:793765/1‑151 (MQ=255)
                                                                                                                                                 ccACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATAACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCAtt  >  1:817350/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATT  >  NC_000913/3260909‑3261203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: