Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,508,657	0	A	G	43.1%	92.1 / 11.4	58	F386S (TTC→TCC)	yhfW	phosphopentomutase‑related metalloenzyme superfamily protein
Reads supporting (aligned to +/- strand):  ref base A (6/27);  new base G (25/0);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 4.74e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCG  >  NC_000913/3508512‑3508804                                                                                                                                                   |                                                                                                                                                     ccACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGaaaaa                                                                                                                                                  <  1:1080926/150‑1 (MQ=255)  cACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGgaaa                                                                                                                                                   >  1:515051/1‑148 (MQ=255)  cACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAAtt                                                                                                                                                <  2:212837/151‑1 (MQ=255)    cGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGgaaaa                                                                                                                                                  >  1:856712/1‑147 (MQ=255)        gttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTcacaca                                                                                                                                          <  1:254284/151‑1 (MQ=255)               aaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGGGGGCGGGGAAAATCACACAAggggac                                                                                                                                   >  1:994299/1‑147 (MQ=255)                    ggAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑CTGCGCGCGGGAAAAATCACACACAGGAGccccc                                                                                                                               >  1:114902/1‑150 (MQ=255)                        agaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGGGCGCGGGAAAAATCAAACACGGGAGCCCCcaca                                                                                                                            >  2:180091/1‑149 (MQ=255)                        agaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATc                                                                                                                          <  1:1140917/151‑1 (MQ=255)                            cAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGGGCGCGGGAAAAATTACACACGGGAGCCCCCCCATCAGa                                                                                                                       >  1:1130842/1‑150 (MQ=255)                            cAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATCACAAACGGTAGCCCCCccaccaca                                                                                                                       >  1:21729/1‑148 (MQ=255)                            cAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATCACAAACCGGAGccccccc                                                                                                                             >  1:550677/1‑142 (MQ=255)                              atatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATCACACACCGGAGGCCCCCCATCAGaaa                                                                                                                     >  2:57823/1‑150 (MQ=255)                              atatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAg                                                                                                                    <  1:1017913/151‑1 (MQ=255)                                atGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATTACACACCGTAACCCCCCCATCAGaaa                                                                                                                     >  2:652809/1‑148 (MQ=255)                                atGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCg                                                                                                                  <  1:84854/151‑1 (MQ=255)                                       tGCTCATGGGCGAGGGAGTAATCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TTGATCGCGGGAAAAATCACAAACCGGATCCCCCCCATAAGGAAACatgg                                                                                                               >  1:409456/1‑147 (MQ=255)                                         cTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATCAAACAAAGGAGTCCCCACCTCAGAAAACgggggggg                                                                                                           >  1:368013/1‑145 (MQ=255)                                          tCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATTACACACGCTAGCCCCCCCAACACAAAAAGTGGTgcg                                                                                                           >  1:975852/1‑148 (MQ=255)                                           cATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGcaca                                                                                                       <  1:807298/151‑1 (MQ=255)                                            aTGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATCAAACACCGTAGCCCCCACATCAGaaaaagggggggg                                                                                                           >  2:478329/1‑136 (MQ=255)                                             tGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGGGCGCGGGAAAAATCACACACGGTAGCCCCCCCATCAGAAAACGGGGGGCGCACAcc                                                                                                     >  2:548199/1‑151 (MQ=255)                                             tGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑GGGCGGGCGGGAAAAATTACAAACGGGAGCCCCCCCATCAGAAAGAGGGGGGGGCACAcc                                                                                                     >  1:59139/1‑151 (MQ=255)                                                     ggTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCg                                                                                             <  2:1036540/151‑1 (MQ=255)                                                       tgtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAAATCACACACGGGAGCCCCCACCTCAGAAAGCGGGGGgcg                                                                                                           >  1:589960/1‑135 (MQ=255)                                                          aaCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGc                                                                                         <  1:1139622/150‑1 (MQ=255)                                                          aaCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCg                                                                                        <  1:732539/151‑1 (MQ=255)                                                           aCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATTACACACGGTAGCCCCCCCATCAGAAAGAGGGGGGGGCACACCGAGCTGCGTAGCg                                                                                        >  2:935641/1‑150 (MQ=255)                                                           aCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATTACACACGGTAGCCCCCACATCAGAAAGCGTGGGGCGCACACCGAGCTGCGGAGc                                                                                         >  2:450272/1‑149 (MQ=255)                                                            cccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGat                                                                                      <  1:77129/151‑1 (MQ=255)                                                                tCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATTACACACGGGAGCCCCCACATCAGAAAACGGGGGGCGCACACCGAGCTGCGGAGCGatc                                                                                     >  2:1093196/1‑148 (MQ=255)                                                                   gATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCt                                                                              <  2:995824/151‑1 (MQ=255)                                                                    aTCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCtt                                                                             <  1:720943/151‑1 (MQ=255)                                                                      cAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGc                                                                           <  1:300169/151‑1 (MQ=255)                                                                       aaaaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCt                                                                          <  2:83646/151‑1 (MQ=255)                                                                             cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATCACACACGGGAGCCCCCCCATCAAAAAACGTGGGGGGCACACCCAGCTGCGGAGCGGAC‑AACCCCTTCTTATaa                                                                     >  1:177122/1‑150 (MQ=255)                                                                             cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATaa                                                                     <  1:904940/150‑1 (MQ=255)                                                                                     gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCAc                                                             <  2:499336/150‑1 (MQ=255)                                                                                      tctcCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGGGCGCGGGAAAAATCACACACGGGAGCCCCCCCCTCAGAAAAAGGGGGGGGGACACCCAGCTGCGTAGCGAAC‑ATACCTTTCTGATAAAACAacac                                                             >  1:1108661/1‑149 (MQ=255)                                                                                         ccTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTgg                                                          <  2:1085165/149‑1 (MQ=255)                                                                                         ccTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTgg                                                          <  2:140443/149‑1 (MQ=255)                                                                                         ccTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGc                                                         <  2:663365/150‑1 (MQ=255)                                                                                            gCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGcacca                                                     <  2:845380/151‑1 (MQ=255)                                                                                              aaaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATCACACACGGGAACCCCCACACCAGAAAACGCGGTGCGCACACCCAGCTGCGTAGCGGTC‑CTCCCTTGCTGATAAACCAGCACTAGc                                                         >  2:956089/1‑145 (MQ=255)                                                                                                aCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TTGCGCAGGGAAAAAATTAAAAACGGTAGCCCCCACAACAGAAAACGTGGGGCGCCCACCCAGCTTCGGAGCGAAC‑AACCCTTTCTGGTAAAACAACAACGGGACCACtt                                                  >  2:933269/1‑150 (MQ=255)                                                                                                aCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTc                                                 <  1:636478/151‑1 (MQ=255)                                                                                                aCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑AGGCGCGCGGGAAAAATCACACACGGTAGCCCCCACATCAGAAAACGGGGGGCGCACACCCAGCTGCGTAGCGATC‑ATCCCTTTCTGATAAAACAACACTGGcaccac                                                    >  2:950391/1‑148 (MQ=255)                                                                                                  cGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTcgc                                               <  2:3534/151‑1 (MQ=255)                                                                                                   ggagggagGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATTACAAACGGGAGCCCCCCCATCAGAAAGCGGGGGGGGCACACCCAGCTGCGTAGCGATC‑ATCCCCTGCTGATAAACCAACACCGGCACCACCTcgc                                               >  1:255306/1‑150 (MQ=255)                                                                                                     agggaggAAAGAAAAGAGCGACCATTTTGTGGCGGATGGC‑CGCGGGAAAAAAGAAACACAGTAGCCCCCCCATCAGGAAACGGGGTGTGCACACCCAGCTGCGTGGCGGTCAACCCCTTCCTG‑TAAAACAGCACCGGCCCCCCCTCCCggg                                            >  1:737054/1‑151 (MQ=255)                                                                                                      gggaggAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGt                                           <  1:883447/151‑1 (MQ=255)                                                                                                           gaaagaaaAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATCAAACACGGGAGCCCCCCCATCAGAAAACGGGGGGCGCACACCCAGCTGCGTAGGGGTC‑ATCCCCTTCTTGTAAAACAACACTGGCCCCACTTCCCGGGGAtgg                                       >  2:186774/1‑150 (MQ=255)                                                                                                                 aaagaGCGACCATTTTGTGGCGG‑TGGGGCGCGGGAAAAATCAAACACGGGAGCCCCCACATCAGAAAACGTGGGGCGCACACCGAGCTGCGTAGCGATC‑ATCCCCTGCTGATAAAACAGCACTGGCCCCACTTCGCGGGGATGGGGGCtg                                 >  2:218510/1‑150 (MQ=255)                                                                                                                         aCCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATg                        <  2:856910/151‑1 (MQ=255)                                                                                                                               ttGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCgg                   <  1:853206/150‑1 (MQ=255)                                                                                                                                 gtggcgg‑tggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGAt                 <  1:136112/150‑1 (MQ=255)                                                                                                                                   ggcgg‑tggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGt              <  2:869334/151‑1 (MQ=255)                                                                                                                                    gcgg‑tggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGtt             >  1:305738/1‑151 (MQ=255)                                                                                                                                           gcgcgcGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTg       >  1:451856/1‑151 (MQ=255)                                                                                                                                             gcgcgGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGAt     >  1:478191/1‑151 (MQ=255)                                                                                                                                                cgGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCg  >  2:131188/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                     CCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCG  >  NC_000913/3508512‑3508804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑