Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,508,6620TA45.8% 90.0 / 26.1 59E384D (GAA→GAT) yhfWphosphopentomutase‑related metalloenzyme superfamily protein
Reads supporting (aligned to +/- strand):  ref base T (6/26);  new base A (27/0);  total (33/26)
Fisher's exact test for biased strand distribution p-value = 2.32e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATC  >  NC_000913/3508513‑3508809
                                                                                                                                                      |                                                                                                                                                    
cACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAAtt                                                                                                                                                     <  2:212837/151‑1 (MQ=255)
      gttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTcacaca                                                                                                                                               <  1:254284/151‑1 (MQ=255)
             aaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGGGGGCGGGGAAAATCACACAAggggac                                                                                                                                        >  1:994299/1‑147 (MQ=255)
                  ggAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑CTGCGCGCGGGAAAAATCACACACAGGAGccccc                                                                                                                                    >  1:114902/1‑150 (MQ=255)
                      agaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGGGCGCGGGAAAAATCAAACACGGGAGCCCCcaca                                                                                                                                 >  2:180091/1‑149 (MQ=255)
                      agaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATc                                                                                                                               <  1:1140917/151‑1 (MQ=255)
                          cAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGGGCGCGGGAAAAATTACACACGGGAGCCCCCCCATCAGa                                                                                                                            >  1:1130842/1‑150 (MQ=255)
                          cAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATCACAAACGGTAGCCCCCccaccaca                                                                                                                            >  1:21729/1‑148 (MQ=255)
                          cAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATCACAAACCGGAGccccccc                                                                                                                                  >  1:550677/1‑142 (MQ=255)
                            atatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATCACACACCGGAGGCCCCCCATCAGaaa                                                                                                                          >  2:57823/1‑150 (MQ=255)
                            atatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAg                                                                                                                         <  1:1017913/151‑1 (MQ=255)
                              atGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATTACACACCGTAACCCCCCCATCAGaaa                                                                                                                          >  2:652809/1‑148 (MQ=255)
                              atGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCg                                                                                                                       <  1:84854/151‑1 (MQ=255)
                                     tGCTCATGGGCGAGGGAGTAATCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TTGATCGCGGGAAAAATCACAAACCGGATCCCCCCCATAAGGAAACatgg                                                                                                                    >  1:409456/1‑147 (MQ=255)
                                       cTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATCAAACAAAGGAGTCCCCACCTCAGAAAACgggggggg                                                                                                                >  1:368013/1‑145 (MQ=255)
                                        tCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATTACACACGCTAGCCCCCCCAACACAAAAAGTGGTgcg                                                                                                                >  1:975852/1‑148 (MQ=255)
                                         cATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGcaca                                                                                                            <  1:807298/151‑1 (MQ=255)
                                          aTGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATCAAACACCGTAGCCCCCACATCAGaaaaagggggggg                                                                                                                >  2:478329/1‑136 (MQ=255)
                                           tGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGGGCGCGGGAAAAATCACACACGGTAGCCCCCCCATCAGAAAACGGGGGGCGCACAcc                                                                                                          >  2:548199/1‑151 (MQ=255)
                                           tGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑GGGCGGGCGGGAAAAATTACAAACGGGAGCCCCCCCATCAGAAAGAGGGGGGGGCACAcc                                                                                                          >  1:59139/1‑151 (MQ=255)
                                                   ggTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCg                                                                                                  <  2:1036540/151‑1 (MQ=255)
                                                     tgtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAAATCACACACGGGAGCCCCCACCTCAGAAAGCGGGGGgcg                                                                                                                >  1:589960/1‑135 (MQ=255)
                                                        aaCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGc                                                                                              <  1:1139622/150‑1 (MQ=255)
                                                        aaCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCg                                                                                             <  1:732539/151‑1 (MQ=255)
                                                         aCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATTACACACGGTAGCCCCCCCATCAGAAAGAGGGGGGGGCACACCGAGCTGCGTAGCg                                                                                             >  2:935641/1‑150 (MQ=255)
                                                         aCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATTACACACGGTAGCCCCCACATCAGAAAGCGTGGGGCGCACACCGAGCTGCGGAGc                                                                                              >  2:450272/1‑149 (MQ=255)
                                                          cccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGat                                                                                           <  1:77129/151‑1 (MQ=255)
                                                              tCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATTACACACGGGAGCCCCCACATCAGAAAACGGGGGGCGCACACCGAGCTGCGGAGCGatc                                                                                          >  2:1093196/1‑148 (MQ=255)
                                                                 gATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCt                                                                                   <  2:995824/151‑1 (MQ=255)
                                                                  aTCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCtt                                                                                  <  1:720943/151‑1 (MQ=255)
                                                                    cAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGc                                                                                <  1:300169/151‑1 (MQ=255)
                                                                     aaaaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCt                                                                               <  2:83646/151‑1 (MQ=255)
                                                                           cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATCACACACGGGAGCCCCCCCATCAAAAAACGTGGGGGGCACACCCAGCTGCGGAGCGGAC‑AACCCCTTCTTATaa                                                                          >  1:177122/1‑150 (MQ=255)
                                                                           cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATaa                                                                          <  1:904940/150‑1 (MQ=255)
                                                                                   gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCAc                                                                  <  2:499336/150‑1 (MQ=255)
                                                                                    tctcCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGGGCGCGGGAAAAATCACACACGGGAGCCCCCCCCTCAGAAAAAGGGGGGGGGACACCCAGCTGCGTAGCGAAC‑ATACCTTTCTGATAAAACAacac                                                                  >  1:1108661/1‑149 (MQ=255)
                                                                                       ccTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTgg                                                               <  2:140443/149‑1 (MQ=255)
                                                                                       ccTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTgg                                                               <  2:1085165/149‑1 (MQ=255)
                                                                                       ccTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGc                                                              <  2:663365/150‑1 (MQ=255)
                                                                                          gCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGcacca                                                          <  2:845380/151‑1 (MQ=255)
                                                                                            aaaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATCACACACGGGAACCCCCACACCAGAAAACGCGGTGCGCACACCCAGCTGCGTAGCGGTC‑CTCCCTTGCTGATAAACCAGCACTAGc                                                              >  2:956089/1‑145 (MQ=255)
                                                                                              aCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TTGCGCAGGGAAAAAATTAAAAACGGTAGCCCCCACAACAGAAAACGTGGGGCGCCCACCCAGCTTCGGAGCGAAC‑AACCCTTTCTGGTAAAACAACAACGGGACCACtt                                                       >  2:933269/1‑150 (MQ=255)
                                                                                              aCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTc                                                      <  1:636478/151‑1 (MQ=255)
                                                                                              aCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑AGGCGCGCGGGAAAAATCACACACGGTAGCCCCCACATCAGAAAACGGGGGGCGCACACCCAGCTGCGTAGCGATC‑ATCCCTTTCTGATAAAACAACACTGGcaccac                                                         >  2:950391/1‑148 (MQ=255)
                                                                                                cGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTcgc                                                    <  2:3534/151‑1 (MQ=255)
                                                                                                 ggagggagGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATTACAAACGGGAGCCCCCCCATCAGAAAGCGGGGGGGGCACACCCAGCTGCGTAGCGATC‑ATCCCCTGCTGATAAACCAACACCGGCACCACCTcgc                                                    >  1:255306/1‑150 (MQ=255)
                                                                                                   agggaggAAAGAAAAGAGCGACCATTTTGTGGCGGATGGC‑CGCGGGAAAAAAGAAACACAGTAGCCCCCCCATCAGGAAACGGGGTGTGCACACCCAGCTGCGTGGCGGTCAACCCCTTCCTG‑TAAAACAGCACCGGCCCCCCCTCCCggg                                                 >  1:737054/1‑151 (MQ=255)
                                                                                                    gggaggAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGt                                                <  1:883447/151‑1 (MQ=255)
                                                                                                         gaaagaaaAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGGAAAAATCAAACACGGGAGCCCCCCCATCAGAAAACGGGGGGCGCACACCCAGCTGCGTAGGGGTC‑ATCCCCTTCTTGTAAAACAACACTGGCCCCACTTCCCGGGGAtgg                                            >  2:186774/1‑150 (MQ=255)
                                                                                                               aaagaGCGACCATTTTGTGGCGG‑TGGGGCGCGGGAAAAATCAAACACGGGAGCCCCCACATCAGAAAACGTGGGGCGCACACCGAGCTGCGTAGCGATC‑ATCCCCTGCTGATAAAACAGCACTGGCCCCACTTCGCGGGGATGGGGGCtg                                      >  2:218510/1‑150 (MQ=255)
                                                                                                                       aCCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATg                             <  2:856910/151‑1 (MQ=255)
                                                                                                                             ttGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCgg                        <  1:853206/150‑1 (MQ=255)
                                                                                                                               gtggcgg‑tggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGAt                      <  1:136112/150‑1 (MQ=255)
                                                                                                                                 ggcgg‑tggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGt                   <  2:869334/151‑1 (MQ=255)
                                                                                                                                  gcgg‑tggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGtt                  >  1:305738/1‑151 (MQ=255)
                                                                                                                                         gcgcgcGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTg            >  1:451856/1‑151 (MQ=255)
                                                                                                                                           gcgcgGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGAt          >  1:478191/1‑151 (MQ=255)
                                                                                                                                              cgGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCg       >  2:131188/1‑151 (MQ=255)
                                                                                                                                                  aaaaTTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCAt   >  1:792740/1‑151 (MQ=255)
                                                                                                                                                   aaaTTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATc  >  1:215714/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
CACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGG‑TGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC‑ATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATC  >  NC_000913/3508513‑3508809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑