Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	89,556	A→G	intergenic (+524/‑78)	cra → / → mraZ	transcriptional repressor‑activator for carbon metabolism/RsmH methytransferase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	89,556	0	A	G	100.0%	294.4 / NA	82	intergenic (+524/‑78)	cra/mraZ	transcriptional repressor‑activator for carbon metabolism/RsmH methytransferase inhibitor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/41);  total (41/41)

TTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTATCG  >  NC_000913/89409‑89701                                                                                                                                                    |                                                                                                                                                  ttttAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGATAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCa                                                                                                                                                 >  1:288885/1‑150 (MQ=255)  tttAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCaa                                                                                                                                                <  1:981343/150‑1 (MQ=255)       aaCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCtttt                                                                                                                                          <  2:345877/151‑1 (MQ=255)          ggTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCt                                                                                                                                       <  1:834230/151‑1 (MQ=255)          ggTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCt                                                                                                                                       >  2:364576/1‑151 (MQ=255)           gTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTc                                                                                                                                      <  1:1037195/151‑1 (MQ=255)            tgatgaCGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTc                                                                                                                                      >  1:742242/1‑150 (MQ=255)             gatgaCGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTTATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCa                                                                                                                                     <  1:187784/150‑1 (MQ=255)                   gATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTcc                                                                                                                                >  1:53142/1‑149 (MQ=255)                    aTGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTa                                                                                                                             <  2:266382/151‑1 (MQ=255)                    aTGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTa                                                                                                                             <  1:177044/151‑1 (MQ=255)                      gAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTaa                                                                                                                            <  1:504401/150‑1 (MQ=255)                        gggATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGCCTTGGCACGCTTTTCCTTACGTCCGTaaa                                                                                                                           >  1:513947/1‑149 (MQ=255)                          gATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTTCTCAGCTCCGTAAACTcc                                                                                                                       >  1:57095/1‑151 (MQ=255)                          gATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTcc                                                                                                                       >  2:653269/1‑151 (MQ=255)                            ttttttCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCtt                                                                                                                     >  2:692654/1‑151 (MQ=255)                             tttttCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCtt                                                                                                                     <  1:104687/150‑1 (MQ=255)                              ttttCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCttt                                                                                                                    <  2:906429/150‑1 (MQ=255)                                 tCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAg                                                                                                                 >  1:1179408/1‑150 (MQ=255)                                    tACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTggg                                                                                                             >  2:734801/1‑151 (MQ=255)                                       aGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGaaa                                                                                                          <  2:1143277/151‑1 (MQ=255)                                       aGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGaaa                                                                                                          <  1:822034/151‑1 (MQ=255)                                       aGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGaaa                                                                                                          <  1:134434/151‑1 (MQ=255)                                        gCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGaaa                                                                                                          >  2:176792/1‑150 (MQ=255)                                             tCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCTTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATtgtg                                                                                                     <  1:1188905/150‑1 (MQ=255)                                             tCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTgg                                                                                                    >  1:187172/1‑151 (MQ=255)                                              cATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTggg                                                                                                   <  1:814631/151‑1 (MQ=255)                                                 aaCGTTAATTTGATTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTTCTCAGCTCCGAAAACTCCTTTCAGAGGGGAATTGTgggg                                                                                                  >  1:932012/1‑149 (MQ=255)                                                   cGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCaaa                                                                                              <  2:645232/151‑1 (MQ=255)                                                   cGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCaaa                                                                                              <  2:1182057/151‑1 (MQ=255)                                                    gTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCaa                                                                                               >  2:240061/1‑149 (MQ=255)                                                     ttAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCTGTGGGAAATTGAGGGGCAAAGt                                                                                            >  2:1120655/1‑151 (MQ=255)                                                     ttAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAACTTTTTCTCAGCTCCGTAAACTCCTTTCAGTGGGGAATTGTGGGGCaaa                                                                                              >  1:511184/1‑149 (MQ=255)                                                        aTTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTggg                                                                                         <  1:770198/151‑1 (MQ=255)                                                                 gCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAgggg                                                                                >  2:539291/1‑151 (MQ=255)                                                                     gTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAg                                                                            <  2:79113/151‑1 (MQ=255)                                                                      ttGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTTCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGGGAgg                                                                           >  1:909595/1‑151 (MQ=255)                                                                      ttGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTg                                                                              <  1:286973/148‑1 (MQ=255)                                                                      ttGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAgg                                                                           >  2:306380/1‑151 (MQ=255)                                                                      ttGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGGGAgg                                                                           >  2:351545/1‑151 (MQ=255)                                                                       tGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAgg                                                                           <  1:159060/150‑1 (MQ=255)                                                                         gACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTg                                                                        <  1:530337/151‑1 (MQ=255)                                                                         gACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTg                                                                        <  1:797007/151‑1 (MQ=255)                                                                          aCGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTTCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTgg                                                                       >  1:524039/1‑151 (MQ=255)                                                                           cGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGc                                                                      >  2:137414/1‑151 (MQ=255)                                                                               aaaTAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGt                                                                  <  2:714075/151‑1 (MQ=255)                                                                                aaTAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGtt                                                                 <  1:421088/151‑1 (MQ=255)                                                                                 aTAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTc                                                                <  1:1101753/151‑1 (MQ=255)                                                                                 aTAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTc                                                                <  1:1174311/151‑1 (MQ=255)                                                                                  tAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCCGTGGGAAATTGTGGGGCCAAGGGGGAATAAGGGGTGAGGCTTGCAAGtt                                                                 >  2:183155/1‑149 (MQ=255)                                                                                      caacGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGGAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCgggg                                                           >  1:157908/1‑151 (MQ=255)                                                                                      caacGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCggg                                                            <  2:122806/150‑1 (MQ=255)                                                                                          gCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAAATCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAg                                                         >  2:84412/1‑149 (MQ=255)                                                                                           cTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCa                                                       >  2:1042765/1‑150 (MQ=255)                                                                                             gATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGGGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACg                                                    >  2:49150/1‑151 (MQ=255)                                                                                               tatTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGGGGGGATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACggt                                                  >  1:858709/1‑149 (MQ=255)                                                                                                atTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTa                                                 <  2:288956/151‑1 (MQ=255)                                                                                                   aGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAAATCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGGGAGGCTGGCATGTTCCGGGGAGCAACGTTAg                                                >  2:1067469/1‑149 (MQ=255)                                                                                                      cGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTTCTCCGCACCGGAAACTCCTTTCAGTGGGGAATTGTGGGGCACAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGGGCAACGGTAGTGaa                                            >  1:779290/1‑150 (MQ=255)                                                                                                      cGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAAt                                           <  1:653120/151‑1 (MQ=255)                                                                                                             aTCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACa                                    <  2:336822/151‑1 (MQ=255)                                                                                                               cGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAg                                   <  1:1077789/150‑1 (MQ=255)                                                                                                               cGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGc                                  <  1:1158855/151‑1 (MQ=255)                                                                                                                gggTTTTTTACCTCGGTATTCCTTGTGACTGGCTTGGCAAACTTTTCCTTAGCTCCGTTAACTCCTTTCCGTGGGAAATTGTGGGGGAAAGAGGGGATAAGGGGGAAGGCTGGCATGTTCCTGGGAGCAACGTTAGTCAATCTCGACa                                    >  1:1162676/1‑148 (MQ=255)                                                                                                                  gTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGAGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCaaa                               <  1:1120402/151‑1 (MQ=255)                                                                                                                     ttttACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAgg                             <  2:856173/150‑1 (MQ=255)                                                                                                                      tttACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAggg                            >  2:1029909/1‑150 (MQ=255)                                                                                                                         aCCTCGGTATGCCTTGTGACTGGCTTGGCAAACTTTTCCTCAGTTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCt                        >  2:205429/1‑151 (MQ=255)                                                                                                                           cTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGGAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCCACGTTAGTCAATCTCGACAGCAAAGGGCGCtt                       >  1:618920/1‑150 (MQ=255)                                                                                                                           cTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCtt                       >  2:964779/1‑150 (MQ=255)                                                                                                                           cTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCtt                       >  2:734592/1‑150 (MQ=255)                                                                                                                             cGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTa                      <  1:461143/149‑1 (MQ=255)                                                                                                                             cGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTa                      <  1:664548/149‑1 (MQ=255)                                                                                                                              ggTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCa                   <  1:1162027/151‑1 (MQ=255)                                                                                                                                 aTGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGGAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAAGGCGCTTATCa                   >  1:51697/1‑148 (MQ=255)                                                                                                                                 aTGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTg                >  1:734408/1‑151 (MQ=255)                                                                                                                                       tgtgACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTa            <  1:608657/149‑1 (MQ=255)                                                                                                                                       tgtgACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTAcc          >  2:271197/1‑151 (MQ=255)                                                                                                                                         tgACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTAccc         >  1:18505/1‑150 (MQ=255)                                                                                                                                           aCTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGtt      <  2:975623/151‑1 (MQ=255)                                                                                                                                              ggCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTATc   <  2:616721/151‑1 (MQ=255)                                                                                                                                               gCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTATCg  >  2:1062798/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                  TTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTATCG  >  NC_000913/89409‑89701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑