Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,021,274	T→C	V350A (GTG→GCG)	ubiB →	regulator of octaprenylphenol hydroxylation, ubiquinone synthesis; regulator of 2'‑N‑acetyltransferase; putative ABC1 family protein kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,021,274	0	T	C	100.0%	257.5 / NA	69	V350A (GTG→GCG)	ubiB	regulator of octaprenylphenol hydroxylation, ubiquinone synthesis; regulator of 2'‑N‑acetyltransferase; putative ABC1 family protein kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/29);  total (40/29)

GGAAAACCCGAAATATATCGGCATTGATTGCGGGATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGTGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGT  >  NC_000913/4021128‑4021418                                                                                                                                                   |                                                                                                                                                 ggAAAACCCGAAATATATCGGCATTGATTGCGGGATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGcc                                                                                                                                               >  2:19688/1‑150 (MQ=255)    aaaCCCGAAATATATCGGCATTGATTGCGGGATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGccacca                                                                                                                                           <  1:739771/151‑1 (MQ=255)    aaaCCCGAAATATATCGGCATTGATTGCGGGATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGccacc                                                                                                                                            >  2:1064278/1‑150 (MQ=255)         cGAAATATATCGGCATTGATTGCGGGATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATAc                                                                                                                                      >  1:265070/1‑151 (MQ=255)            aaTATATCGGCATTGATTGCGGGATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCa                                                                                                                                    >  1:458150/1‑150 (MQ=255)              tatatCGGCATTGATTGCGGGATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACg                                                                                                                                 >  2:1075769/1‑151 (MQ=255)                 atCGGCATTGATTGCGGGATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTg                                                                                                                              >  2:3599/1‑151 (MQ=255)                   cGGCATTGATTGCGGGATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGaa                                                                                                                            <  1:1158584/151‑1 (MQ=255)                   cGGCATTGATTGCGGGATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGa                                                                                                                             <  2:951724/150‑1 (MQ=255)                   cGGCATTGATTGCGGGATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGa                                                                                                                             >  2:863075/1‑150 (MQ=255)                      cATTGATTGCGGGATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAag                                                                                                                           >  1:453489/1‑149 (MQ=255)                       attgattgCGGGATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGt                                                                                                                        >  2:1006965/1‑151 (MQ=255)                           attgCGGGATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCg                                                                                                                     >  2:188966/1‑150 (MQ=255)                              gCGGGATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAAtt                                                                                                                 >  2:990217/1‑151 (MQ=255)                               cGGGATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAAttt                                                                                                                <  1:986905/151‑1 (MQ=255)                                gggATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTg                                                                                                               >  2:1059532/1‑151 (MQ=255)                                 ggATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGc                                                                                                              >  1:866387/1‑151 (MQ=255)                                  gATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGcc                                                                                                             >  2:974976/1‑151 (MQ=255)                                          gCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTAc                                                                                                     <  2:807748/151‑1 (MQ=255)                                            tCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACgg                                                                                                   >  2:170366/1‑151 (MQ=255)                                             cGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACg                                                                                                    <  1:949723/149‑1 (MQ=255)                                                 aaaCAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCtgt                                                                                              >  2:694098/1‑151 (MQ=255)                                                 aaaCAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCtgt                                                                                              >  2:706360/1‑151 (MQ=255)                                                 aaaCAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCt                                                                                                <  1:823717/149‑1 (MQ=255)                                                  aaCAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCAAATTTGCCATTCGTACGGTCtgtg                                                                                             <  2:383314/151‑1 (MQ=255)                                                       agaagaTAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCt                                                                                        <  1:511691/151‑1 (MQ=255)                                                         aagaTAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTAt                                                                                      >  2:1155413/1‑151 (MQ=255)                                                               aaCGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTg                                                                                 >  1:1126326/1‑150 (MQ=255)                                                                 cGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTg                                                                                 >  1:736374/1‑148 (MQ=255)                                                                   cTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTgaga                                                                              >  2:99631/1‑149 (MQ=255)                                                                     aTCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGaaa                                                                            <  1:992512/149‑1 (MQ=255)                                                                     aTCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGaaa                                                                            <  2:762863/149‑1 (MQ=255)                                                                     aTCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAAcc                                                                          >  2:116857/1‑151 (MQ=255)                                                                       cTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGc                                                                        >  2:544183/1‑151 (MQ=255)                                                                         ggCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCg                                                                         <  1:38714/148‑1 (MQ=255)                                                                         ggCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCt                                                                       <  2:915988/150‑1 (MQ=255)                                                                          gCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGTATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTgg                                                                     >  2:954503/1‑151 (MQ=255)                                                                              aaaaCTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCg                                                                  >  1:541047/1‑150 (MQ=255)                                                                              aaaaCTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGa                                                                 <  2:648842/151‑1 (MQ=255)                                                                               aaaCTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGaa                                                                <  2:945912/151‑1 (MQ=255)                                                                                aaCTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGaaa                                                               <  1:707383/151‑1 (MQ=255)                                                                                     tATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTc                                                           <  2:348053/150‑1 (MQ=255)                                                                                      aTCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGt                                                         <  2:164559/151‑1 (MQ=255)                                                                                         gCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTg                                                      >  2:631306/1‑151 (MQ=255)                                                                                          ccTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTgg                                                     >  1:830521/1‑151 (MQ=255)                                                                                             tcttTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGAc                                                   >  1:375437/1‑150 (MQ=255)                                                                                             tcttTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGAc                                                   >  2:1166261/1‑150 (MQ=255)                                                                                               tttAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATg                                                >  1:558109/1‑151 (MQ=255)                                                                                                ttAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGt                                               >  2:174664/1‑151 (MQ=255)                                                                                                  aaTCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTAc                                             >  1:183215/1‑151 (MQ=255)                                                                                                   aTCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACt                                            <  1:665014/151‑1 (MQ=255)                                                                                                   aTCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACt                                            <  1:98495/151‑1 (MQ=255)                                                                                                    tCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTg                                           >  1:96125/1‑151 (MQ=255)                                                                                                           tATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATc                                    >  2:1020374/1‑151 (MQ=255)                                                                                                            aTCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATc                                    <  1:50620/150‑1 (MQ=255)                                                                                                            aTCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATc                                    <  1:869102/150‑1 (MQ=255)                                                                                                                 aaaGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGttt                               >  1:529952/1‑150 (MQ=255)                                                                                                                 aaaGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGttt                               >  2:108339/1‑150 (MQ=255)                                                                                                                 aaaGTGGCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTa                              <  1:1064030/151‑1 (MQ=255)                                                                                                                      ggCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATAc                          <  2:422701/150‑1 (MQ=255)                                                                                                                      ggCAGAGCTACACGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACg                         <  1:28884/151‑1 (MQ=255)                                                                                                                               acacGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTc                  <  2:866588/149‑1 (MQ=255)                                                                                                                                cacGTCGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCt               >  2:158829/1‑151 (MQ=255)                                                                                                                                    tCGATTCTGGCTGGTCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCa            <  1:399544/150‑1 (MQ=255)                                                                                                                                     cGATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAat          >  2:976356/1‑151 (MQ=255)                                                                                                                                      gATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAat          >  2:70189/1‑150 (MQ=255)                                                                                                                                      gATTCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAat          >  1:382584/1‑150 (MQ=255)                                                                                                                                         tCTGGCTGGGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATg       <  1:298150/150‑1 (MQ=255)                                                                                                                                             gctggGCGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGt  <  2:1126578/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 GGAAAACCCGAAATATATCGGCATTGATTGCGGGATTGTTGGCTCGCTAAACAAAGAAGATAAACGCTATCTGGCAGAAAACTTTATCGCCTTCTTTAATCGCGACTATCGCAAAGTGGCAGAGCTACACGTCGATTCTGGCTGGGTGCCACCAGATACCAACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGT  >  NC_000913/4021128‑4021418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑