Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,106,405	T→C	intergenic (+85/‑64)	cpxP → / → fieF	inhibitor of the cpx response; periplasmic adaptor protein/ferrous iron and zinc transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,106,405	0	T	C	100.0%	324.5 / NA	84	intergenic (+85/‑64)	cpxP/fieF	inhibitor of the cpx response; periplasmic adaptor protein/ferrous iron and zinc transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/37);  total (47/37)

GACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTGATTAAAATT  >  NC_000913/4106257‑4106552                                                                                                                                                     |                                                                                                                                                    gacgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCtgccgc                                                                                                                                                   >  1:409234/1‑151 (MQ=255)  acgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTgccgct                                                                                                                                                  >  1:277310/1‑151 (MQ=255)   cgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCtt                                                                                                                                                 >  2:1148844/1‑151 (MQ=255)   cgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCtt                                                                                                                                                 >  1:538217/1‑151 (MQ=255)   cgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCtt                                                                                                                                                 >  1:31836/1‑151 (MQ=255)     cAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTaa                                                                                                                                               <  2:168474/151‑1 (MQ=255)     cAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTa                                                                                                                                                >  2:393847/1‑150 (MQ=255)      aaTGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAAt                                                                                                                                              >  1:481675/1‑151 (MQ=255)      aaTGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAAt                                                                                                                                              <  2:124264/151‑1 (MQ=255)               aaaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGc                                                                                                                                       >  1:492775/1‑149 (MQ=255)               aaaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGc                                                                                                                                       >  1:451716/1‑149 (MQ=255)                aaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAga                                                                                                                                    <  2:6002/151‑1 (MQ=255)                     cATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAgatgat                                                                                                                                <  2:1164253/150‑1 (MQ=255)                      aTCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGAttt                                                                                                                              >  2:695947/1‑151 (MQ=255)                         gTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCt                                                                                                                           >  1:602915/1‑151 (MQ=255)                           tGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTc                                                                                                                         >  1:615639/1‑151 (MQ=255)                              aGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGt                                                                                                                      >  2:41014/1‑151 (MQ=255)                              aGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGt                                                                                                                      >  1:999706/1‑151 (MQ=255)                                 tATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTtat                                                                                                                   >  1:1145807/1‑151 (MQ=255)                                  aTTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTtat                                                                                                                   >  1:1139050/1‑150 (MQ=255)                                    tGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACt                                                                                                                >  2:1099961/1‑151 (MQ=255)                                    tGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACt                                                                                                                >  1:625/1‑151 (MQ=255)                                     gAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACt                                                                                                                >  1:53208/1‑150 (MQ=255)                                              cAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGtt                                                                                                      <  2:17230/151‑1 (MQ=255)                                              cAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGtt                                                                                                      >  2:93079/1‑151 (MQ=255)                                                        tCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCACTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCggg                                                                                             >  2:943810/1‑150 (MQ=255)                                                         cccAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGa                                                                                            <  1:738888/150‑1 (MQ=255)                                                          ccAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGt                                                                                          <  1:430278/151‑1 (MQ=255)                                                            agtagtAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTAt                                                                                        <  2:1166552/151‑1 (MQ=255)                                                            agtagtAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTAt                                                                                        <  2:379407/151‑1 (MQ=255)                                                              tagtaAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTAttt                                                                                      >  1:613547/1‑151 (MQ=255)                                                               agtaAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTAttt                                                                                      >  1:121779/1‑150 (MQ=255)                                                               agtaAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTAttt                                                                                      <  2:1110771/150‑1 (MQ=255)                                                               agtaAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTAttt                                                                                      <  2:390402/150‑1 (MQ=255)                                                                   aaCCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCa                                                                                                         >  1:563998/1‑127 (MQ=255)                                                                   aaCCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCa                                                                                                         <  2:564136/127‑1 (MQ=255)                                                                    aCCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGaat                                                                                >  1:311895/1‑151 (MQ=255)                                                                       cTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGaatca                                                                              >  2:527909/1‑150 (MQ=255)                                                                            ttCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTAt                                                                                    <  1:45417/139‑1 (MQ=255)                                                                            ttCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTAt                                                                                    >  2:45427/1‑139 (MQ=255)                                                                            ttCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCtt                                                                         >  1:249602/1‑150 (MQ=255)                                                                              ccTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTAt                                                                       <  2:535646/150‑1 (MQ=255)                                                                                 tGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATgg                                                                     <  2:544297/149‑1 (MQ=255)                                                                                   ccATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACgg                                                                 >  1:1099112/1‑151 (MQ=255)                                                                                    cATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGc                                                                <  2:879411/151‑1 (MQ=255)                                                                                       aGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTgg                                                             >  2:525953/1‑151 (MQ=255)                                                                                       aGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTgg                                                             <  1:385778/151‑1 (MQ=255)                                                                                       aGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTgg                                                             <  2:784631/151‑1 (MQ=255)                                                                                         acacCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTc                                                           >  2:717174/1‑151 (MQ=255)                                                                                          cacCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCa                                                          <  1:1019547/151‑1 (MQ=255)                                                                                             cATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTc                                                       >  2:235703/1‑151 (MQ=255)                                                                                             cATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTc                                                       >  2:45219/1‑151 (MQ=255)                                                                                              aTCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCg                                                      <  1:124849/151‑1 (MQ=255)                                                                                                cccTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCggg                                                    >  1:305181/1‑151 (MQ=255)                                                                                                 ccTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGggc                                                   >  2:508305/1‑151 (MQ=255)                                                                                                         cccccACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGAt                                             <  2:385430/149‑1 (MQ=255)                                                                                                         cccccACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATtg                                           <  2:31845/151‑1 (MQ=255)                                                                                                           cccACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATtgct                                         >  2:884430/1‑151 (MQ=255)                                                                                                           cccACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATtgct                                         <  1:393753/151‑1 (MQ=255)                                                                                                           cccACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATtgct                                         >  2:1007239/1‑151 (MQ=255)                                                                                                              acaTGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCg                                      <  2:778457/151‑1 (MQ=255)                                                                                                              acaTGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCg                                      >  2:8988/1‑151 (MQ=255)                                                                                                                aTGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGAc                                    >  1:137217/1‑151 (MQ=255)                                                                                                                aTGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGAc                                    >  2:445643/1‑151 (MQ=255)                                                                                                                  gCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACgg                                  <  2:249683/151‑1 (MQ=255)                                                                                                                     gtgGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGc                                 <  2:254867/149‑1 (MQ=255)                                                                                                                        ggggTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATgg                            >  2:960192/1‑151 (MQ=255)                                                                                                                        ggggTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATgg                            <  2:481793/151‑1 (MQ=255)                                                                                                                          ggTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCt                          <  2:862065/151‑1 (MQ=255)                                                                                                                           gTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCtt                         <  2:451826/151‑1 (MQ=255)                                                                                                                           gTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCtt                         <  2:538350/151‑1 (MQ=255)                                                                                                                           gTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCtt                         >  1:1178851/1‑151 (MQ=255)                                                                                                                             tttttttATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTc                        <  1:814893/150‑1 (MQ=255)                                                                                                                                 tttATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCgctgc                   >  1:667733/1‑151 (MQ=255)                                                                                                                                 tttATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCgctgc                   >  1:235565/1‑151 (MQ=255)                                                                                                                                    aTCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTAt                >  2:380017/1‑151 (MQ=255)                                                                                                                                      ccTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTAtt               <  1:865039/150‑1 (MQ=255)                                                                                                                                      ccTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTg              <  2:230789/151‑1 (MQ=255)                                                                                                                                      ccTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTg              <  2:149849/151‑1 (MQ=255)                                                                                                                                        tCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCt            >  1:846746/1‑151 (MQ=255)                                                                                                                                         cAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTg           <  1:1099710/151‑1 (MQ=255)                                                                                                                                          aaTTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTg           >  2:611496/1‑150 (MQ=255)                                                                                                                                             ttGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTGAtt        >  2:642760/1‑150 (MQ=255)                                                                                                                                               gCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTGATTaaa     <  1:406509/151‑1 (MQ=255)                                                                                                                                               gCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTGATTaa      >  2:583607/1‑150 (MQ=255)                                                                                                                                                ccTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTGATTaaaa    <  2:407959/151‑1 (MQ=255)                                                                                                                                                  tgccgcTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTGATTAAAAtt  >  2:130546/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTGATTAAAATT  >  NC_000913/4106257‑4106552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑