Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,114,622	A→G	S320P (TCC→CCC)	glpX ←	fructose 1,6‑bisphosphatase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,114,622	0	A	G	100.0%	217.0 / NA	55	S320P (TCC→CCC)	glpX	fructose 1,6‑bisphosphatase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/23);  total (32/23)

TTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATC  >  NC_000913/4114475‑4114754                                                                                                                                                    |                                                                                                                                     ttttCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCt                                                                                                                                    <  2:42241/150‑1 (MQ=255) ttttCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTg                                                                                                                                   >  2:1157032/1‑151 (MQ=255)   ttCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGa                                                                                                                                  >  2:181354/1‑150 (MQ=255)          gTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATgcggcg                                                                                                                          >  2:560324/1‑151 (MQ=255)            tttGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAGGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGa                                                                                                                         <  1:872942/150‑1 (MQ=255)            tttGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGa                                                                                                                         >  2:80100/1‑150 (MQ=255)             ttgattgaCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAAt                                                                                                                       >  1:658676/1‑151 (MQ=255)             ttgattgaCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAAt                                                                                                                       >  1:64417/1‑151 (MQ=255)              tgattgaCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGACCGCTCAATCGACCAAATCAATCAGAGGATGTGCACCTGCCTTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATgcgg                                                                                                                            >  1:810970/1‑145 (MQ=255)                 ttgaCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGCATGGTg                                                                                                                   >  2:972753/1‑151 (MQ=255)                   gaCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGc                                                                                                                  <  1:743404/150‑1 (MQ=255)                      ttGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTg                                                                                                               >  2:1028642/1‑150 (MQ=255)                          cTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTg                                                                                                          >  1:1041688/1‑151 (MQ=255)                          cTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTg                                                                                                          >  2:969111/1‑151 (MQ=255)                           ttATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGc                                                                                                         >  1:528993/1‑151 (MQ=255)                           ttATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGc                                                                                                         >  2:496028/1‑151 (MQ=255)                             aTCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCcg                                                                                                       >  1:1093046/1‑151 (MQ=255)                                      ctcCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcagc                                                                                              >  2:860001/1‑151 (MQ=255)                                        ccGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcagcag                                                                                            <  1:452317/151‑1 (MQ=255)                                        ccGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcagc                                                                                              >  2:759773/1‑149 (MQ=255)                                        ccGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcagc                                                                                              <  1:375419/149‑1 (MQ=255)                                              aaaaaaaTTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTc                                                                                      <  1:884113/151‑1 (MQ=255)                                                  aaaTTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTa                                                                                  <  1:441236/151‑1 (MQ=255)                                                   aaTTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGt                                                                                   >  1:964699/1‑149 (MQ=255)                                                   aaTTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAg                                                                                 <  2:862142/151‑1 (MQ=255)                                                        cAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTcgcg                                                                            >  2:9067/1‑151 (MQ=255)                                                           tCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGat                                                                          >  2:585332/1‑150 (MQ=255)                                                            cccGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGatat                                                                        >  1:517490/1‑151 (MQ=255)                                                            cccGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGata                                                                         <  1:66075/150‑1 (MQ=255)                                                            cccGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGata                                                                         <  2:601903/150‑1 (MQ=255)                                                             ccGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGGTTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATAtt                                                                       <  2:1084507/151‑1 (MQ=255)                                                               gAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGc                                                                     >  2:82910/1‑151 (MQ=255)                                                                   gACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCttt                                                                 <  1:80076/151‑1 (MQ=255)                                                                      tGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCg                                                              >  2:458972/1‑151 (MQ=255)                                                                        gAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCgg                                                             >  2:628742/1‑150 (MQ=255)                                                                         aaGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCt                                                           <  2:1123569/151‑1 (MQ=255)                                                                         aaGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCt                                                           <  2:366101/151‑1 (MQ=255)                                                                          aGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTa                                                          <  2:1187692/151‑1 (MQ=255)                                                                              tCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGc                                                      >  2:1057261/1‑151 (MQ=255)                                                                               cAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGcc                                                     >  1:441626/1‑151 (MQ=255)                                                                                       caaatcaaTCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCcagca                                             <  2:499831/151‑1 (MQ=255)                                                                                          atcaatcaGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCcagc                                              >  1:764576/1‑147 (MQ=255)                                                                                                        gtgCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAAt                             >  1:146984/1‑150 (MQ=255)                                                                                                        gtgCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATa                            <  1:438236/151‑1 (MQ=255)                                                                                                          gCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATAc                           <  1:1024514/150‑1 (MQ=255)                                                                                                              cTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTg                      >  2:341583/1‑151 (MQ=255)                                                                                                                 cATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCa                   <  1:915356/151‑1 (MQ=255)                                                                                                                  aTTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGc                    >  1:447461/1‑149 (MQ=255)                                                                                                                   tttCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGc                    >  2:1009554/1‑148 (MQ=255)                                                                                                                     tCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCagag                <  2:3650/150‑1 (MQ=255)                                                                                                                      ccGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAgaga               <  2:939246/150‑1 (MQ=255)                                                                                                                       cGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGagaag             <  2:529126/151‑1 (MQ=255)                                                                                                                              ttGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGAc        <  1:851554/149‑1 (MQ=255)                                                                                                                               tGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGtt     >  1:148714/1‑151 (MQ=255)                                                                                                                                  gATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATc  >  2:578129/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                     TTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATC  >  NC_000913/4114475‑4114754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑