Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,480,290	T→C	G187G (GGT→GGC)	yjgN →	DUF898 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,480,290	0	T	C	100.0%	364.4 / NA	91	G187G (GGT→GGC)	yjgN	DUF898 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/47);  total (44/47)

TATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATAAAATATGCCATTCTCGCATTTTTAGC  >  NC_000913/4480143‑4480436                                                                                                                                                    |                                                                                                                                                   tATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCAtt                                                                                                                                                 >  1:656234/1‑151 (MQ=255)    gAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGtt                                                                                                                                              <  1:303569/151‑1 (MQ=255)     aaaGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGttt                                                                                                                                             <  2:357112/151‑1 (MQ=255)      aaGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTc                                                                                                                                            <  1:1096239/151‑1 (MQ=255)      aaGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTc                                                                                                                                            <  2:914592/151‑1 (MQ=255)      aaGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTc                                                                                                                                            >  1:1155102/1‑151 (MQ=255)         ggTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCAt                                                                                                                                         >  1:889419/1‑151 (MQ=255)          gTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCAtt                                                                                                                                        >  1:228833/1‑151 (MQ=255)             cTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTgg                                                                                                                                      >  2:854726/1‑150 (MQ=255)              tggtggGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTGCCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTg                                                                                                                                       <  2:265871/148‑1 (MQ=255)              tggtggGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGt                                                                                                                                     >  1:960083/1‑150 (MQ=255)               ggtgggtgACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTgg                                                                                                                                      <  1:472863/148‑1 (MQ=255)               ggtgggtgACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGt                                                                                                                                     <  2:212884/149‑1 (MQ=255)                gtgggtgACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTAtt                                                                                                                                  <  2:1126204/151‑1 (MQ=255)                 tgggtgACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTAttt                                                                                                                                 >  1:409511/1‑151 (MQ=255)                  gggtgACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTAtttt                                                                                                                                <  1:350422/151‑1 (MQ=255)                  gggtgACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTAtttt                                                                                                                                <  1:689960/151‑1 (MQ=255)                    gtgACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTa                                                                                                                              <  2:16871/151‑1 (MQ=255)                      gACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAAt                                                                                                                            >  2:224924/1‑151 (MQ=255)                      gACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAAt                                                                                                                            <  1:1130099/151‑1 (MQ=255)                        ccTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATgg                                                                                                                          >  2:670331/1‑151 (MQ=255)                         cTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGt                                                                                                                         >  1:550135/1‑151 (MQ=255)                           ttttCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTa                                                                                                                        <  2:702230/150‑1 (MQ=255)                            tttCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACt                                                                                                                      >  2:408395/1‑151 (MQ=255)                                ttGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTtat                                                                                                                  >  2:607823/1‑151 (MQ=255)                                   ccGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTtatata                                                                                                               <  1:947239/151‑1 (MQ=255)                                   ccGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTtatat                                                                                                                >  2:139074/1‑150 (MQ=255)                                    cGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTtatat                                                                                                                >  1:581032/1‑149 (MQ=255)                                    cGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTtatat                                                                                                                >  2:915207/1‑149 (MQ=255)                                    cGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAg                                                                                                              <  1:928490/151‑1 (MQ=255)                                     gATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGt                                                                                                             <  2:1092383/151‑1 (MQ=255)                                      aTTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTc                                                                                                            >  2:443619/1‑151 (MQ=255)                                         ttAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTgg                                                                                                         >  2:540567/1‑151 (MQ=255)                                          tAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTgg                                                                                                         <  1:1138825/150‑1 (MQ=255)                                            aTGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTa                                                                                                       >  1:125241/1‑150 (MQ=255)                                                 cATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGt                                                                                                 >  1:981412/1‑151 (MQ=255)                                                   ttGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGttt                                                                                               >  2:605603/1‑151 (MQ=255)                                                    tGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGtttt                                                                                              >  1:244269/1‑151 (MQ=255)                                                      ggATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTtct                                                                                            >  2:105448/1‑151 (MQ=255)                                                       gATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTtctc                                                                                           <  1:432280/151‑1 (MQ=255)                                                            tgACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTgg                                                                                        >  1:535643/2‑149 (MQ=255)                                                            ggACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAg                                                                                      <  2:748276/151‑1 (MQ=255)                                                              aCTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTa                                                                                    <  2:1154538/151‑1 (MQ=255)                                                               cTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCGGGTAATGAGTTTTCTCTGGAGTa                                                                                    <  1:275239/150‑1 (MQ=255)                                                                tGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAAt                                                                                  >  2:913048/1‑151 (MQ=255)                                                                tGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAAt                                                                                  >  2:776002/1‑151 (MQ=255)                                                                    ttCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATAcc                                                                               <  2:291894/150‑1 (MQ=255)                                                                              cGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGt                                                                      <  2:322487/149‑1 (MQ=255)                                                                               gACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGtata                                                                   <  2:18524/151‑1 (MQ=255)                                                                               gACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGtat                                                                    >  2:702194/1‑150 (MQ=255)                                                                                aCAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATAc                                                                  <  2:960308/151‑1 (MQ=255)                                                                                   aaGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATc                                                               <  2:1155363/151‑1 (MQ=255)                                                                                    aGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCg                                                              >  1:890568/1‑151 (MQ=255)                                                                                         cTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTc                                                          >  2:766466/1‑150 (MQ=255)                                                                                            ccTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAgg                                                      <  1:702023/151‑1 (MQ=255)                                                                                            ccTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAg                                                       <  2:463696/150‑1 (MQ=255)                                                                                            ccTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAg                                                       <  2:919230/150‑1 (MQ=255)                                                                                             cTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCaa                                                        <  1:768171/148‑1 (MQ=255)                                                                                              tGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTg                                                    >  1:158886/1‑151 (MQ=255)                                                                                               gCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTg                                                    <  1:405137/150‑1 (MQ=255)                                                                                                 cAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGaa                                                  >  1:318917/1‑150 (MQ=255)                                                                                                     aGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTa                                              <  2:777425/150‑1 (MQ=255)                                                                                                          aaGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGAtact                                        >  2:870770/1‑151 (MQ=255)                                                                                                             tagtGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACg                                     >  2:24017/1‑151 (MQ=255)                                                                                                              agtGTTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACg                                     <  1:663022/150‑1 (MQ=255)                                                                                                               gtgtTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTa                                   <  2:1000682/151‑1 (MQ=255)                                                                                                                tgtTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTAt                                  >  2:213098/1‑151 (MQ=255)                                                                                                                 gtTATTATATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTAtt                                 >  1:871805/1‑151 (MQ=255)                                                                                                                   tattatATCCATTGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGt                               >  2:125292/1‑151 (MQ=255)                                                                                                                   tattatATCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGt                               >  1:1140619/1‑151 (MQ=255)                                                                                                                       atatCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGtata                            >  2:477512/1‑150 (MQ=255)                                                                                                                       atatCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATaa                           <  2:244322/151‑1 (MQ=255)                                                                                                                         atCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATaaaa                         <  1:301044/151‑1 (MQ=255)                                                                                                                         atCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATaaa                          <  1:1106085/150‑1 (MQ=255)                                                                                                                         atCCATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATaaa                          <  1:745627/150‑1 (MQ=255)                                                                                                                           ccATGGTTCTGATGGCAGTAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATAAAata                       <  1:139039/151‑1 (MQ=255)                                                                                                                           ccATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGtata                            >  1:251126/1‑146 (MQ=255)                                                                                                                           ccATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATAAAat                        >  1:652129/1‑150 (MQ=255)                                                                                                                            cATGGTTCTGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATAAAatat                      <  1:568365/151‑1 (MQ=255)                                                                                                                                    tGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATAAAATATGCCATtct              >  1:657189/1‑151 (MQ=255)                                                                                                                                    tGATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATAAAATATGCCATtct              >  2:448211/1‑151 (MQ=255)                                                                                                                                     gATGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATAAAATATGCCATtctc             <  2:413909/151‑1 (MQ=255)                                                                                                                                       tGGCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATAAAATATGCCATTCTCGc           >  2:475579/1‑151 (MQ=255)                                                                                                                                        ggCAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATAAAATATGCCATTCTCGCa          <  2:1091383/151‑1 (MQ=255)                                                                                                                                          cAATAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATAAAATATGCCATTCTCGCAtt        >  1:1026597/1‑151 (MQ=255)                                                                                                                                            aTAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATAAAATATGCCATTCTCGCAtttt      <  1:408298/151‑1 (MQ=255)                                                                                                                                            aTAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATAAAATATGCCATTCTCGCAtttt      >  2:478680/1‑151 (MQ=255)                                                                                                                                            aTAGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATAAAATATGCCATTCTCGCAtttt      <  1:670175/151‑1 (MQ=255)                                                                                                                                              aGTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATAAAATATGCCATTCTCGCATTTTTa    <  2:889623/151‑1 (MQ=255)                                                                                                                                               gTTGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATAAAATATGCCATTCTCGCATTTTTAg   <  2:550272/151‑1 (MQ=255)                                                                                                                                                 tGGCATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATAAAATATGCCATTCTCGCATTTTTAGc  <  2:172188/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTCTCTGGAGTAATACCAGTTTCGGTATACATCGTTTCAAGGTGAAATTAGATACTACGTATTGTATAAAATATGCCATTCTCGCATTTTTAGC  >  NC_000913/4480143‑4480436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑