Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	483,535	C→T	G290E (GGG→GAG)	acrB ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	483,535	0	C	T	100.0%	182.4 / NA	47	G290E (GGG→GAG)	acrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/28);  total (19/28)

GTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTC  >  NC_000913/483390‑483674                                                                                                                                                  |                                                                                                                                            gTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGa                                                                                                                                        <  1:226305/151‑1 (MQ=255)    aaTAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCg                                                                                                                                     >  1:685371/1‑151 (MQ=255)     aTAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCgg                                                                                                                                    >  1:980069/1‑151 (MQ=255)        gggaTTTTCCCGAACGGAGAGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAg                                                                                                                                 <  2:224129/149‑1 (MQ=255)        gagaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATctcc                                                                                                                                           >  2:246101/1‑141 (MQ=255)             tttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCgg                                                                                                                             <  2:1015115/150‑1 (MQ=255)                   gAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAATGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCg                                                                                                                      <  1:833626/151‑1 (MQ=255)                     aCGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGtt                                                                                                                    >  2:1121530/1‑151 (MQ=255)                       ggCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAATCCGGTTGGCCGTTa                                                                                                                   >  2:883962/1‑150 (MQ=255)                          gtggtgTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAAc                                                                                                                >  1:347963/1‑150 (MQ=255)                                tCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCg                                                                                                          >  1:885724/1‑150 (MQ=255)                                 cGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCgat                                                                                                        <  1:794923/151‑1 (MQ=255)                                  gTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCgatg                                                                                                       <  2:707194/151‑1 (MQ=255)                                     tGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGatgatg                                                                                                    >  2:545555/1‑151 (MQ=255)                                     tGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGatgatg                                                                                                    >  2:458177/1‑151 (MQ=255)                                       ggTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTc                                                                                                  <  1:617178/151‑1 (MQ=255)                                          aaaCAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTa                                                                                               <  2:542727/151‑1 (MQ=255)                                          aaaCAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTa                                                                                               <  2:1129769/151‑1 (MQ=255)                                          aaaCAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTa                                                                                               <  2:556661/151‑1 (MQ=255)                                           aaCAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAg                                                                                              <  2:553526/151‑1 (MQ=255)                                                   tCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTcacc                                                                                      >  1:1173620/1‑151 (MQ=255)                                                      gACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCaccacc                                                                                   <  2:177760/151‑1 (MQ=255)                                                        cccGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCAccc                                                                                  >  1:1113766/1‑150 (MQ=255)                                                           gACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGTCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCt                                                                              >  2:843262/1‑151 (MQ=255)                                                              gggAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCa                                                                            >  1:672717/1‑150 (MQ=255)                                                                      cGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTcgcg                                                                   <  1:1055078/151‑1 (MQ=255)                                                                       ggTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTcgcg                                                                   >  2:1016170/1‑150 (MQ=255)                                                                          tCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACg                                                                <  2:1062831/150‑1 (MQ=255)                                                                            cATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGt                                                               >  1:396433/1‑149 (MQ=255)                                                                            cATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCa                                                             <  1:961559/151‑1 (MQ=255)                                                                                ttCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACgc                                                          >  1:695271/1‑150 (MQ=255)                                                                                      aGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACgcagcagc                                                    >  1:1016795/1‑150 (MQ=255)                                                                                      aGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACgcagcagc                                                    >  2:1085678/1‑150 (MQ=255)                                                                                       gTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCAc                                                  <  1:238761/151‑1 (MQ=255)                                                                                       gTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCAc                                                  <  2:83013/151‑1 (MQ=255)                                                                                         tCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCAcgc                                                >  1:607700/1‑151 (MQ=255)                                                                                          cAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCAcgcg                                               <  1:394001/151‑1 (MQ=255)                                                                                           aGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCgg                                              <  1:1019058/151‑1 (MQ=255)                                                                                            gCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCggg                                             >  2:62675/1‑151 (MQ=255)                                                                                                 gATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCa                                        <  2:825873/151‑1 (MQ=255)                                                                                                  aTTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCAt                                       <  1:619571/151‑1 (MQ=255)                                                                                                          aGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTc                               <  2:662978/151‑1 (MQ=255)                                                                                                                aTCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACttt                          <  2:348045/150‑1 (MQ=255)                                                                                                                  ccAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTca                        <  2:685538/150‑1 (MQ=255)                                                                                                                     gcgcGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTcagcag                    <  1:1015934/151‑1 (MQ=255)                                                                                                                                ccGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGa         <  2:872773/151‑1 (MQ=255)                                                                                                                                       ccAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTc  <  1:256789/151‑1 (MQ=255)                                                                                                                                       ccAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTc  <  2:581386/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                            GTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTC  >  NC_000913/483390‑483674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑