Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	487,092	T→C	L186P (CTG→CCG)	mscK →	mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	487,092	0	T	C	100.0%	268.8 / NA	72	L186P (CTG→CCG)	mscK	mechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/36);  total (36/36)

GTTGCCCAGGCGCTGGACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCTGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGC  >  NC_000913/486944‑487238                                                                                                                                                     |                                                                                                                                                   gTTGCCCAGGCGCTGGACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCgg                                                                                                                                                  >  2:1183523/1‑151 (MQ=255)   tGCCCAGGCGCTGGACGATTTGCAAAACGCACAAAACAATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGa                                                                                                                                                 <  2:363448/150‑1 (MQ=255)        aGGCGCTGGACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGAc                                                                                                                                           >  1:392704/1‑151 (MQ=255)                  cGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGc                                                                                                                                 <  1:99746/151‑1 (MQ=255)                  cGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGc                                                                                                                                 <  2:384390/151‑1 (MQ=255)                   gATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCg                                                                                                                                <  2:22245/151‑1 (MQ=255)                   gATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCg                                                                                                                                >  1:214909/1‑151 (MQ=255)                       tGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCgaga                                                                                                                             <  2:546249/150‑1 (MQ=255)                            aaCGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCt                                                                                                                       >  1:949993/1‑151 (MQ=255)                               gCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTAc                                                                                                                    >  2:439700/1‑151 (MQ=255)                                cacaAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACg                                                                                                                   <  1:794216/151‑1 (MQ=255)                                 acaAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGt                                                                                                                  <  2:532876/151‑1 (MQ=255)                                   aaaaCGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTcc                                                                                                                >  2:471556/1‑151 (MQ=255)                                     aaCGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTcc                                                                                                                >  1:232681/1‑149 (MQ=255)                                        gATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCccagcc                                                                                                           >  1:803620/1‑151 (MQ=255)                                             ggCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTTATGTCGGCGAGACAGCCTTACGTCCCAGCCAGaa                                                                                                       <  1:202250/150‑1 (MQ=255)                                              gCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAg                                                                                                     <  2:32548/151‑1 (MQ=255)                                              gCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAg                                                                                                     <  2:171373/151‑1 (MQ=255)                                                      taACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATg                                                                                             >  2:193440/1‑151 (MQ=255)                                                        aCAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTCAATGc                                                                                            >  2:1106236/1‑150 (MQ=255)                                                         cagccagcTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGc                                                                                            >  1:391397/1‑149 (MQ=255)                                                         cagccagcTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGc                                                                                            >  1:153910/1‑149 (MQ=255)                                                             cagcTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGccc                                                                                      >  2:511767/1‑151 (MQ=255)                                                              agcTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCca                                                                                     <  2:1159167/151‑1 (MQ=255)                                                                cTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCcagc                                                                                   >  1:1127359/1‑151 (MQ=255)                                                                  ggTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCcagcag                                                                                 <  1:1174339/151‑1 (MQ=255)                                                                  ggTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCcagcag                                                                                 <  1:850214/151‑1 (MQ=255)                                                                   gTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAgg                                                                                >  1:91294/1‑151 (MQ=255)                                                                     ttCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGc                                                                               <  1:511638/150‑1 (MQ=255)                                                                     ttCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGc                                                                               <  2:1148858/150‑1 (MQ=255)                                                                     ttCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCg                                                                              >  2:976177/1‑151 (MQ=255)                                                                       cGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGtt                                                                            <  2:474937/151‑1 (MQ=255)                                                                          tACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGc                                                                          <  2:148779/150‑1 (MQ=255)                                                                           aCAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCt                                                                         <  1:497977/150‑1 (MQ=255)                                                                           aCAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTg                                                                        <  1:398141/151‑1 (MQ=255)                                                                              gACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAAt                                                                     <  1:721470/151‑1 (MQ=255)                                                                                cGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGc                                                                   <  2:22498/151‑1 (MQ=255)                                                                                 gCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCg                                                                  >  1:1052053/1‑151 (MQ=255)                                                                                   aGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCgg                                                                 >  2:219670/1‑150 (MQ=255)                                                                                   aGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCgg                                                                 >  1:552817/1‑150 (MQ=255)                                                                                   aGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCgg                                                                 >  1:914601/1‑150 (MQ=255)                                                                                        gAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTg                                                           >  2:753527/1‑151 (MQ=255)                                                                                        gAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTg                                                           >  2:1148725/1‑151 (MQ=255)                                                                                          aCGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATACAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGAc                                                         <  2:503890/151‑1 (MQ=255)                                                                                                 cAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCagcagc                                                  >  1:219706/1‑151 (MQ=255)                                                                                                  aaaaTGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCg                                                 >  2:280345/1‑151 (MQ=255)                                                                                                   aaaTGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGt                                                >  1:327444/1‑151 (MQ=255)                                                                                                       gCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTaaa                                             <  1:640368/150‑1 (MQ=255)                                                                                                         gATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGc                                          >  2:576800/1‑151 (MQ=255)                                                                                                            gTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCt                                        <  1:527246/150‑1 (MQ=255)                                                                                                            gTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTg                                       >  2:519261/1‑151 (MQ=255)                                                                                                              ataACGCTTCGCAGCAGCTGCAACAAATTCGCGGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTgg                                      >  1:547676/1‑150 (MQ=255)                                                                                                              ataACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTgg                                      <  2:1174522/150‑1 (MQ=255)                                                                                                                 aCGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAgg                                  <  2:530348/151‑1 (MQ=255)                                                                                                                  cGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAggg                                 <  1:652838/151‑1 (MQ=255)                                                                                                                   gCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGa                                <  1:996346/151‑1 (MQ=255)                                                                                                                      tCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAac                              >  1:537558/1‑150 (MQ=255)                                                                                                                         cagcagCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCg                          >  2:639469/1‑151 (MQ=255)                                                                                                                              gcTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTg                     <  2:601917/151‑1 (MQ=255)                                                                                                                                 gCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCa                   <  1:535565/150‑1 (MQ=255)                                                                                                                                 gCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCa                   <  2:1127199/150‑1 (MQ=255)                                                                                                                                       aaTTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATAcc            >  2:233874/1‑151 (MQ=255)                                                                                                                                       aaTTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATAcc            >  1:761776/1‑151 (MQ=255)                                                                                                                                           cGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTg         <  1:391583/150‑1 (MQ=255)                                                                                                                                            gCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGc        >  1:646172/1‑150 (MQ=255)                                                                                                                                              aGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCaaa     <  2:261076/151‑1 (MQ=255)                                                                                                                                               gtcgtcCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCaaaa    <  1:193397/151‑1 (MQ=255)                                                                                                                                                 cgtcCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAg   <  1:506824/150‑1 (MQ=255)                                                                                                                                                 cgtcCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGc  >  1:219857/1‑151 (MQ=255)                                                                                                                                                 cgtcCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGc  <  2:391490/151‑1 (MQ=255)                                                                                                                                                  gtcCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGc  >  1:351088/1‑150 (MQ=255)                                                                                                                                                  gtcCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGc  >  2:244227/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GTTGCCCAGGCGCTGGACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCTGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGC  >  NC_000913/486944‑487238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑