Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	514,850	A→G	L4L (TTA→CTA)	ybbJ ←	inner membrane protein; stimulator of the QmcA suppressor of ftsH‑htpX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	514,850	0	A	G	100.0%	224.2 / NA	57	L4L (TTA→CTA)	ybbJ	inner membrane protein; stimulator of the QmcA suppressor of ftsH‑htpX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/36);  total (21/36)

AACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTG  >  NC_000913/514703‑514997                                                                                                                                                    |                                                                                                                                                    aaCCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCCCCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCt                                                                                                                                                   <  2:21360/150‑1 (MQ=255)           cACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCt                                                                                                                                        >  1:995912/1‑151 (MQ=255)                 ccagACCACCAGGCGGGTAATCCCCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTcc                                                                                                                                  <  2:809981/151‑1 (MQ=255)                 ccagACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTcc                                                                                                                                  >  1:134430/1‑151 (MQ=255)                      cccccaGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGtt                                                                                                                               <  2:65361/146‑1 (MQ=255)                              ccGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCt                                                                                                                     >  1:261620/1‑151 (MQ=255)                               cGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTCGATGAACGACCATTCGCCCCATCATGGCTGAGTCCGTTTGTTGGCACTg                                                                                                                    >  1:69553/1‑151 (MQ=255)                                ggTAATCCCCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCt                                                                                                                     <  1:918456/149‑1 (MQ=255)                                ggTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTg                                                                                                                    >  1:594209/1‑150 (MQ=255)                                ggTAACCCCCGCTCCCACGCCGCTCCACAACAAATAACCATTCCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGt                                                                                                                   <  1:525182/151‑1 (MQ=255)                                    aTCCCCGCTACCACGCCGCTCCACAACAAAAAACCATTTCCGCCCGGCATCTCGGCTGCCAGCAGAAAGCCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTg                                                                                                                    <  1:1038255/146‑1 (MQ=255)                                       aCCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCAc                                                                                                            >  2:589223/1‑151 (MQ=255)                                        ccGCTGCCACGCCGCTCCACAAAAAAAACCCATTCCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCAcc                                                                                                           <  1:545957/151‑1 (MQ=255)                                            tGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCt                                                                                                       >  2:802016/1‑151 (MQ=255)                                                aCGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGc                                                                                                   >  2:111278/1‑151 (MQ=255)                                                aCGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGc                                                                                                   >  1:846878/1‑151 (MQ=255)                                                   ccgcTCCACAACAAATACCCATTCCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAAt                                                                                                <  1:1069049/151‑1 (MQ=255)                                                                aaaaaCCATTCCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGAc                                                                                   <  1:1095482/148‑1 (MQ=255)                                                                   aaCCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGAccc                                                                                 <  2:1041669/150‑1 (MQ=255)                                                                     ccaatcccGCCCAGAATTTCGGCTCCCCGCAACAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCAt                                                                              <  2:205353/145‑1 (MQ=255)                                                                     ccATTCCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCAt                                                                              <  1:110613/151‑1 (MQ=255)                                                                              cccAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGgctgct                                                                     >  2:811938/1‑151 (MQ=255)                                                                              cccAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGgctgct                                                                     <  2:1076926/151‑1 (MQ=255)                                                                                      ctcGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCct                                                                <  1:111244/148‑1 (MQ=255)                                                                                        cGGCTGCCAGCCGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCct                                                                <  1:146229/146‑1 (MQ=255)                                                                                        cGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCctct                                                              <  2:1037682/148‑1 (MQ=255)                                                                                        cGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTaa                                                            <  1:941905/150‑1 (MQ=255)                                                                                        cGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTa                                                             <  2:1106632/149‑1 (MQ=255)                                                                                          gCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATgg                                                         <  1:136225/151‑1 (MQ=255)                                                                                              ccAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGcatc                                                     <  2:1191390/151‑1 (MQ=255)                                                                                              ccAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGcat                                                      <  2:684871/150‑1 (MQ=255)                                                                                                 gcagcaAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGcatcat                                                   <  2:556294/150‑1 (MQ=255)                                                                                                  cagcaAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGcatca                                                    <  1:1030362/148‑1 (MQ=255)                                                                                                            ccgAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCt                                       <  1:496230/151‑1 (MQ=255)                                                                                                            ccgAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCt                                       >  1:216900/1‑151 (MQ=255)                                                                                                            ccgAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCt                                       <  2:231212/151‑1 (MQ=255)                                                                                                             cgAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTg                                      <  2:980825/151‑1 (MQ=255)                                                                                                             cgAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTg                                      >  1:728013/1‑151 (MQ=255)                                                                                                               agaCTGAGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGtt                                    <  1:77699/151‑1 (MQ=255)                                                                                                                     aGCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTgga                              <  2:289610/151‑1 (MQ=255)                                                                                                                      gCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTgg                               >  2:293859/1‑149 (MQ=255)                                                                                                                      gCCAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTgg                               >  1:169115/1‑149 (MQ=255)                                                                                                                        cAGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTggagg                            >  1:92516/1‑150 (MQ=255)                                                                                                                         aGAAAATATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGaa                          <  2:1049312/151‑1 (MQ=255)                                                                                                                           aaaaTATGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAAcc                        <  1:667494/151‑1 (MQ=255)                                                                                                                              atatGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGAt                     <  2:885100/151‑1 (MQ=255)                                                                                                                              atatGTGGATGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGAt                     <  2:1106727/151‑1 (MQ=255)                                                                                                                                      aTGAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGt              >  2:438348/1‑150 (MQ=255)                                                                                                                                        gAACGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAAc           >  1:25058/1‑151 (MQ=255)                                                                                                                                           cGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCt        <  2:44565/151‑1 (MQ=255)                                                                                                                                           cGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCt        >  2:716690/1‑151 (MQ=255)                                                                                                                                           cGACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCt        >  1:706263/1‑151 (MQ=255)                                                                                                                                            gACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCtt       >  2:24313/1‑151 (MQ=255)                                                                                                                                            gACCATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCtt       <  1:801837/151‑1 (MQ=255)                                                                                                                                              ccATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCg     <  1:1140535/151‑1 (MQ=255)                                                                                                                                               cATTAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCgg    <  2:949615/151‑1 (MQ=255)                                                                                                                                                 ttAGCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGgtg  >  2:758550/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    AACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTG  >  NC_000913/514703‑514997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑