Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 134,458 T→C V24A (GTC→GCC)  yacL → UPF0231 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913134,4580TC100.0% 263.9 / NA 74V24A (GTC→GCC) yacLUPF0231 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/35);  total (39/35)

CTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGAT  >  NC_000913/134312‑134605
                                                                                                                                                  |                                                                                                                                                   
ctcCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGGGCGTATGTCCATGGGGCATGAAGTGGCCggg                                                                                                                                                 >  1:210269/1‑151 (MQ=255)
 tcCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCg                                                                                                                                                   <  2:1132370/148‑1 (MQ=255)
        aGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGtt                                                                                                                                         <  2:375822/151‑1 (MQ=255)
              cGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATg                                                                                                                                    >  1:95101/1‑150 (MQ=255)
               gATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGaa                                                                                                                                  <  1:237182/151‑1 (MQ=255)
                  aaTTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAaga                                                                                                                                <  2:448196/150‑1 (MQ=255)
                  aaTTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAag                                                                                                                                 >  1:206014/1‑149 (MQ=255)
                         tCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTgaa                                                                                                                          >  1:513062/1‑149 (MQ=255)
                            aTGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTgaaagaa                                                                                                                      >  2:530377/1‑150 (MQ=255)
                            aTGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTgaaagaa                                                                                                                      >  2:103272/1‑150 (MQ=255)
                               gcgcAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAAcc                                                                                                                  >  1:583937/1‑151 (MQ=255)
                                 gcAAGGCGATTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTg                                                                                                                <  1:169674/150‑1 (MQ=255)
                                   aaTGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGc                                                                                                              >  1:86696/1‑151 (MQ=255)
                                    aTGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGcc                                                                                                             >  1:488435/1‑151 (MQ=255)
                                      gCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCctt                                                                                                           <  2:644359/151‑1 (MQ=255)
                                      gCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCctt                                                                                                           <  2:851369/151‑1 (MQ=255)
                                      gCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCct                                                                                                            <  1:644814/150‑1 (MQ=255)
                                      gCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCct                                                                                                            <  1:1033820/150‑1 (MQ=255)
                                          tttCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCcttgctt                                                                                                       >  1:1072233/1‑151 (MQ=255)
                                           ttCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCcttgcttg                                                                                                      >  1:340658/1‑151 (MQ=255)
                                           ttCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCcttgctt                                                                                                       <  2:496878/150‑1 (MQ=255)
                                             cGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTtga                                                                                                     <  1:377990/150‑1 (MQ=255)
                                                  cATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGt                                                                                               <  2:581526/151‑1 (MQ=255)
                                                   aTTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTg                                                                                              >  2:651950/1‑151 (MQ=255)
                                                      gCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGa                                                                                            >  1:184794/1‑150 (MQ=255)
                                                      gCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGa                                                                                            >  2:677451/1‑150 (MQ=255)
                                                       ccGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAAc                                                                                          >  1:151571/1‑151 (MQ=255)
                                                          ggAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAag                                                                                       <  1:804225/151‑1 (MQ=255)
                                                              cAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAagcagc                                                                                   >  1:852627/1‑151 (MQ=255)
                                                                gagGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAgcgc                                                                                 <  2:414629/151‑1 (MQ=255)
                                                                       atacTATTGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACt                                                                          <  1:964580/149‑1 (MQ=255)
                                                                         acTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGa                                                                        <  2:453634/151‑1 (MQ=255)
                                                                          cTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGa                                                                        >  1:297344/1‑150 (MQ=255)
                                                                            aTGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGaa                                                                       >  2:980613/1‑149 (MQ=255)
                                                                            aTGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGaa                                                                       <  1:677291/149‑1 (MQ=255)
                                                                             tGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAgg                                                                    >  2:6791/1‑151 (MQ=255)
                                                                             tGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAgg                                                                    >  1:400075/1‑151 (MQ=255)
                                                                              ggATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGt                                                                   >  1:363847/1‑151 (MQ=255)
                                                                               gATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTa                                                                  <  1:876038/151‑1 (MQ=255)
                                                                                aTTACGAATTTCTGCGCGATATTACCGGAGGGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGt                                                                   >  2:723368/1‑149 (MQ=255)
                                                                                  tACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCg                                                               >  1:326179/1‑151 (MQ=255)
                                                                                    cGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGaa                                                             <  2:124842/151‑1 (MQ=255)
                                                                                     gAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAAc                                                            >  1:1176354/1‑151 (MQ=255)
                                                                                      aaTTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACg                                                           >  2:922626/1‑151 (MQ=255)
                                                                                       aTTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACgg                                                          >  1:836376/1‑151 (MQ=255)
                                                                                       aTTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACgg                                                          <  1:918577/151‑1 (MQ=255)
                                                                                             gcgcGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCt                                                      <  1:414173/149‑1 (MQ=255)
                                                                                             gcgcGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTgg                                                    <  2:297733/151‑1 (MQ=255)
                                                                                             gcgcGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTg                                                     <  1:318382/150‑1 (MQ=255)
                                                                                               gcgATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCa                                                  <  1:992945/151‑1 (MQ=255)
                                                                                                   tatTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgg                                              >  2:927755/1‑151 (MQ=255)
                                                                                                   tatTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgg                                              >  2:1066013/1‑151 (MQ=255)
                                                                                                     ttACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggc                                            >  2:517247/1‑151 (MQ=255)
                                                                                                       aCCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggca                                           <  2:534647/150‑1 (MQ=255)
                                                                                                       aCCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggc                                            >  1:679763/1‑149 (MQ=255)
                                                                                                        ccGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcagg                                         >  1:1085756/1‑151 (MQ=255)
                                                                                                        ccGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcagg                                         <  2:717330/151‑1 (MQ=255)
                                                                                                         cGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGGTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcaggg                                        <  1:523649/151‑1 (MQ=255)
                                                                                                          ggAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcagggc                                       >  2:1150/1‑151 (MQ=255)
                                                                                                               ggTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGaa                                  <  1:28901/151‑1 (MQ=255)
                                                                                                                  aaaGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATac                               <  1:751589/151‑1 (MQ=255)
                                                                                                                    aGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAATAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATacac                             >  2:217951/1‑151 (MQ=255)
                                                                                                                          gTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCtgtg                       <  2:451100/151‑1 (MQ=255)
                                                                                                                            aTGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtgga                     <  2:654572/151‑1 (MQ=255)
                                                                                                                                ccATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtggatgg                  >  1:788852/1‑150 (MQ=255)
                                                                                                                                 cATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtggatgg                  <  1:668684/149‑1 (MQ=255)
                                                                                                                                  aTGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACg               <  2:1048330/151‑1 (MQ=255)
                                                                                                                                  aTGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACg               <  1:409948/151‑1 (MQ=255)
                                                                                                                                    ggggCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACgg              <  1:855653/150‑1 (MQ=255)
                                                                                                                                    ggggCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACgg              >  2:821692/1‑150 (MQ=255)
                                                                                                                                             aGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTg    >  1:599705/1‑151 (MQ=255)
                                                                                                                                               tGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGa   >  1:635231/1‑150 (MQ=255)
                                                                                                                                               tGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGAt  >  1:984582/1‑151 (MQ=255)
                                                                                                                                               tGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGAt  >  1:215110/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGAT  >  NC_000913/134312‑134605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: